Stimulatory effects of ghrelin on spontaneous contractions in the rat myometrium

WOS: 000276227500005Ghrelin, a recently isolated hormone, has been reported to have modulatory effects on smooth muscle contractility. In this study, we investigated the effects of ghrelin on spontaneous contractions in the rat uterus in vitro. Myometrium strips were removed from Wistar rats following decapitation and placed in a jacketed tissue bath. After initiation of spontaneous contractions, control contractions were recorded for 10 min and various concentrations of ghrelin were added to the tissue bath cumulatively. The Wilcoxon signed ranks test was used for statistical analysis. Application of ghrelin augmented the spontaneous contractions in myometrial strips. The mean peak amplitudes of contractions were 2.69 +/- 0.32 g (n = 6), 2.71 +/- 0.31 g (n = 6), 2.92 +/- 0.36 g (n = 6), and 3.46 +/- 0.24 g (n = 6) under control conditions and after application of 0.01 mu M, 0.1 mu M, and 1 mu M ghrelin, respectively. The mean frequencies of contractions were 5.17 +/- 0.75 (n = 6), 5.17 +/- 0.75 (n = 6), 5.33 +/- 0.48 (n = 6), and 8.0 +/- 0.77 (n = 6) under control conditions and after application of 0.01 mu M, 0.1 mu M, and 1 mu M ghrelin, respectively. The increase in amplitude and frequency of contractions was significant only after application of 1 mu M ghrelin (P < 0.05). Data from this study demonstrated that ghrelin induces spontaneous contractions in a dose-dependent manner in the rat myometrium.Firat University Scientific ResearchFirat University [FUBAP-922]This study was financially supported by Firat University Scientific Research Projects Unit (FUBAP-922)

Effects of Different Light Curing Units/Modes on the Microleakage of Flowable Composite Resins

Objectives The aim of this in vitro study was to evaluate the influence of different light curing units and modes on microleakage of flowable composite resins. Methods Eighty Class V cavities were prepared in buccal and lingual surfaces of 40 extracted human premolars with cervical wall located in dentin and the occlusal wall in enamel. These teeth were randomly assigned into two groups (n=20) and restored with different flowable composites; Group I: Esthet-X Flow, Group II: Grandio Flow. Each group was randomly divided into four subgroups; while the samples of the first subgroup were polymerized with conventional Halogen light, the rest of them were polymerized with different curing modes of Light Emitting Diode (LED). The second subgroup was polymerized with fast-curing; the third subgroup with pulse-curing and those of the fourth subgroup with step-curing modes of LED. After the samples were thermocycled and immersed in dye, they were longitudinally sectioned. Dye penetration was assessed under a stereomicroscope. Data were analyzed by Kruskal-Wallis and Mann-Whitney U tests. Results None of the restorations showed leakage on enamel margins. On dentin margins no significant differences were observed between flowable composite resins polymerized with halogen light (P>.05). While step curing mode of LED presented significant differences between the resins, the difference was insignificant when fast-curing and pulse-curing mode of LED were used. No statistically significant differences were observed between curing units for Esthet-X Flow samples. For Grandio Flow samples, only step-curing mode of LED caused statistically higher leakage scores than halogen and other curing modes of LED (P<.05). Conclusions The effect of curing units’ type and curing mode on flowable composite resin leakage might be material-dependent.PubMe

Efektivitas Implementasi Permainan Aung-carbon-card Pada Materi Pelajaran Senyawa Karbon

Tujuan dalam penelitian adalah mengetahui keefektifan, tingkat kemudahan dan tingkat ketertarikan siswa penggunaan media permainan Aung-Carbon-Card (ACC) dalam pembelajaran struktur senyawa karbon. Penelitian ini menggunakan jenis penelitian eksperimen. Sampel yang digunakan yakni siswa kelas X IPA-1dianggap sebagai kelompok konrol (KC), kelas X IPA-2 (E1), kelas X IPA-3 (E2) dan kelas IPA-4 (E3) dijadikan kelompok eksperimen. Sumber data penelitian ini diperoleh dari tes, angket dan observasi. Instrumen yang digunakan sebelumnya dilakukan uji instrumen untuk mengetahui validitas dan reliabilitas. Data yangdiperoleh dilakukan uji normalitas data. Uji hipotesis dilakukan dengan perhitungan uji statistik uji T two sampel independent menggunakan SPSS 17. Hasil penelitian diperoleh: 1). Pembelajaran senyawa hidrokarbon menggunakan media permainan kartu ACC lebih efektif dibuktikan pada hasil tes dengan uji T, nilai Thitung &gt; Ttabel. 2) Kartu ACC menarik perhatian siswa dalam mempelajari pokok bahasan senyawa karbon dibuktikan dengan hasil angket persentase siswa yang menyatakan permainan kartu ACC hal yang menarik. Hasil persentase E1 88%, E2 85% dan E3 74%. Hasil perhitungan terhadap respon siswa masing-masing kelas eksperimen terhadap KC Thitung &gt; Ttabel. 3). Media permainan kartu ACC dapat membantu mempermudah siswa dalam mempelajari pokok bahasan senyawa dengan hasil angket persentase siswa yang menyatakan bahwa lebih mudah memahami materi dengan menggunakan ACC E1= 76%, E2 = 85% dan E3 = 81%. 4). Media permainan kartu ACC sesuai dan tepat jika digunakan dibuktikan dengan observasi dengan nilai Thitung &gt; Ttabel

Alternative Splicing at a NAGNAG Acceptor Site as a Novel Phenotype Modifier

Approximately 30% of alleles causing genetic disorders generate premature termination codons (PTCs), which are usually associated with severe phenotypes. However, bypassing the deleterious stop codon can lead to a mild disease outcome. Splicing at NAGNAG tandem splice sites has been reported to result in insertion or deletion (indel) of three nucleotides. We identified such a mechanism as the origin of the mild to asymptomatic phenotype observed in cystic fibrosis patients homozygous for the E831X mutation (2623G>T) in the CFTR gene. Analyses performed on nasal epithelial cell mRNA detected three distinct isoforms, a considerably more complex situation than expected for a single nucleotide substitution. Structure-function studies and in silico analyses provided the first experimental evidence of an indel of a stop codon by alternative splicing at a NAGNAG acceptor site. In addition to contributing to proteome plasticity, alternative splicing at a NAGNAG tandem site can thus remove a disease-causing UAG stop codon. This molecular study reveals a naturally occurring mechanism where the effect of either modifier genes or epigenetic factors could be suspected. This finding is of importance for genetic counseling as well as for deciding appropriate therapeutic strategies

Recurrence of primary sclerosing cholangitis after liver transplantation – analysing the European Liver Transplant Registry and beyond

Liver transplantation for primary sclerosing cholangitis (PSC) can be complicated by recurrence of PSC (rPSC). This may compromise graft survival but the effect on patient survival is less clear. We investigated the effect of post-transplant rPSC on graft and patient survival in a large European cohort. Registry data from the European Liver Transplant Registry regarding all first transplants for PSC between 1980 and 2015 were supplemented with detailed data on rPSC from 48 out of 138 contributing transplant centres, involving 1,549 patients. Bayesian proportional hazards models were used to investigate the impact of rPSC and other covariates on patient and graft survival. Recurrence of PSC was diagnosed in 259 patients (16.7%) after a median follow-up of 5.0 years (quantile 2.5%-97.5%: 0.4–18.5), with a significant negative impact on both graft (HR 6.7; 95% CI 4.9–9.1) and patient survival (HR 2.3; 95% CI 1.5–3.3). Patients with rPSC underwent significantly more re-transplants than those without rPSC (OR 3.6, 95% CI 2.7–4.8). PSC recurrence has a negative impact on both graft and patient survival, independent of transplant-related covariates. Recurrence of PSC leads to higher number of re-transplantations and a 33% decrease in 10-year graft survival

CONVERSION FROM CALCINEURIN INHIBITOR-BASED TO EVEROLIMUS-BASED IMMUNOSUPPRESSION IN LIVER TRANSPLANT RECIPIENTS: LONG-TERM OUTCOME

WOS: 000411688503325