Katatere bağlı inatçı sol ana koroner arter spazmı

Fifty one year old female patient was taken into the catheter laboratory for coronary angiography with the diagnosis of typical angina pectoris (CCS Class 2). A 6F JL4 Judkins catheter was placed into the left coronary artery.Immediately after sitting into the left main artery, damping in the pressure tracing was seen and catheter was removed from left main coronary artery (Figure 1). There was a 90% stenosis in the left coronary artery ostium which was seen in the non selectively taken image. LAD and Cx were seemed to be normal. 200 micrograms of nitroglycerin was non selectively administered for the possibility of left coronay artery spasm. 5F JL4 catheter was placed into the left coronary system very delicately. Because damping in the pressure recording recurred, catheter was removed. After administrating 200 micrograms of nitroglycerin a 5F JL4 catheter with two side holes was placed delicately. The procedure was terminated because of the pressure damping and chest pain. RCA was seen to be normal. After taken into the coronary unit, beta blocker therapy was switched to calcium channel blocker therapy and 24 hours of intravenous nitroglycerine infusion was administered. A multislice CT was planned to evaluate the suspected lesion in the left main coronary ostium. In the MSCT was reported that left main coronary ostium was normal and patient was treated medically (Figure 2). The patients with left main coronary lesions are the most risky patients in terms of complication. Therefore, pressure damping should be evaluated immediately after placing into the left coronary system. Damping is entity which is often seen in patients with severe left coronary lesions. Another finding suggestive of severe left main lesion is that no reflux of radiocontrast agent into the aorta is seen. In patients with no other obstructive lesions in the other coronary vessels before preceding to the diagnosis of "isolated coronary ostial lesion", the possibility of catheter induced coronary spasm should be evaluated.This spasm can persist, as occurred in our case, even after recurrent nitroglycerin administration and attempts for placing into the left system with delicate maneuvers and smaller catheters. Multislice CT should be kept in mind to evaluate the left coronary ostium in these group of patients.MSCT is an assisting imaging modality for diagnosis in these group of patients (1,2).Fifty one year old female patient was taken into the catheter laboratory for coronary angiography with the diagnosis of typical angina pectoris (CCS Class 2). A 6F JL4 Judkins catheter was placed into the left coronary artery.Immediately after sitting into the left main artery, damping in the pressure tracing was seen and catheter was removed from left main coronary artery (Figure 1). There was a 90% stenosis in the left coronary artery ostium which was seen in the non selectively taken image. LAD and Cx were seemed to be normal. 200 micrograms of nitroglycerin was non selectively administered for the possibility of left coronay artery spasm. 5F JL4 catheter was placed into the left coronary system very delicately. Because damping in the pressure recording recurred, catheter was removed. After administrating 200 micrograms of nitroglycerin a 5F JL4 catheter with two side holes was placed delicately. The procedure was terminated because of the pressure damping and chest pain. RCA was seen to be normal. After taken into the coronary unit, beta blocker therapy was switched to calcium channel blocker therapy and 24 hours of intravenous nitroglycerine infusion was administered. A multislice CT was planned to evaluate the suspected lesion in the left main coronary ostium. In the MSCT was reported that left main coronary ostium was normal and patient was treated medically (Figure 2). The patients with left main coronary lesions are the most risky patients in terms of complication. Therefore, pressure damping should be evaluated immediately after placing into the left coronary system. Damping is entity which is often seen in patients with severe left coronary lesions. Another finding suggestive of severe left main lesion is that no reflux of radiocontrast agent into the aorta is seen. In patients with no other obstructive lesions in the other coronary vessels before preceding to the diagnosis of "isolated coronary ostial lesion", the possibility of catheter induced coronary spasm should be evaluated.This spasm can persist, as occurred in our case, even after recurrent nitroglycerin administration and attempts for placing into the left system with delicate maneuvers and smaller catheters. Multislice CT should be kept in mind to evaluate the left coronary ostium in these group of patients.MSCT is an assisting imaging modality for diagnosis in these group of patients (1,2)

Borax regulates iron chaperone‐ and autophagy‐mediated ferroptosis pathway in glioblastoma cells

Glioblastoma (GBM) is classified as a stage-IV glioma. Unfortunately, there are currently no curative treatments for GBM. Poly(rC)-binding protein 1 (PCBP1) is a cytosolic iron chaperone with diverse functions. PCBP1 is also known to regulate autophagy, but the role of PCBP1 in ferroptosis, iron-dependent cell death pathway, remains unrevealed in GBM cells. Here, we investigated the effects of borax, a boron compound, on the ferroptosis signaling pathway mediated by PCBP1 and autophagy. The study analyzed cell viability, proliferation, and cell cycle on U87-MG and HMC3 cells to investigate the effects of borax. After determining the cytotoxic concentrations of borax, morphological analyzes and measurement of PCBP1, Beclin1, malondialdehyde (MDA), glutathione (GSH), glutathione peroxidase 4 (GPx4) and acyl-CoA synthetase long chain family member 4 (ACSL4) levels were performed. Finally, expression levels of PCBP1, Beclin1, GPx4 and ACSL4, and caspase-3/7 activity were determined. We found that borax reduced U87-MG cell viability in a concentration- and time-dependent manner. Additionally, borax altered cell proliferation and remarkably reduced S phase in the U87-MG cells and exhibited selectivity by having an opposite effect on normal cells (HMC3). According to DAPI staining, borax caused nuclear deficits in U87-MG cells. The result showed that borax in U87-MG cells induced reduction of the PCBP1, GSH, and GPx4 and enhancement of Beclin1, MDA, and ACSL4. Furthermore, borax triggered apoptosis by activating caspase 3/7 in U87-MG cells. Our study indicated that the borax has potential as an anticancer treatment for GBM via regulating PCBP1/Beclin1/GPx4/ACSL4 signaling pathways.</p

The evaluation of both the expression and serum protein levels of Caspase-3 gene in patients with different degrees of SARS-CoV2 infection

To evaluate the effects of Caspase-3 (CASP3) gene expression and serum levels on preventing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. A total of 41 individuals (male: 21; female: 20) with SARS-CoV-2 infection were included in the current study. Hemograms were examined from patient blood samples, and CASP3 gene expression levels were detected. Also, human CASP3 levels were determined from the serum samples of patients. The mean age of patients was 56.220 +/- 18.937 years. Significant differences were detected among all groups for CASP3 2-Delta Delta Ct (p = 0.014) and CASP3 concentration (p = 0.024). The relationship between CASP3 2-Delta Delta Ct levels and hemoglobin (p = 0.023), between CASP3 2-Delta Delta Ct levels and C-reactive protein (CRP) (p = 0.001), between CASP3 2-Delta Delta Ct levels and ferritin (p = 0.003), between CASP3 2-Delta Delta Ctlevels and lactate dehydrogenase (p = 0.001), and between CASP3 2-Delta Delta Ct levels and SpO(2) (p = 0.006) were statistically significant. Also, the relationship between CASP3 concentration levels and SpO(2) was statistically significant (p < 0.046). The CASP3 gene and/or its products have an important function to prevent injury caused by SARS-CoV-2 infection. They play crucial roles in maintaining cellular homeostasis and viability. Perhaps CASP3 levels may provide information about the severity of the disease.Duzce UniversityDuzce UniversityDuzce University coordinators of scientific research projectsWOS:0007082951000012-s2.0-85117183718PubMed: 3458574

Hematologic Parameters and Angiographic Progression of Coronary Atherosclerosis

Hematologic parameters have prognostic importance in cardiovascular disease. However, the relation between atherosclerosis progression and hematologic parameters is not well defined. A total of 394 patients requiring repeat coronary angiography were included in the study. According to angiography, patients were divided into 2 groups, progressive (n = 196) and nonprogressive (n = 198) diseases. Hematologic parameters including mean platelet volume (MPV) and neutrophil/lymphocyte (N/L) ratio were measured. Glucose, creatinine, and cholesterol were significantly higher in the progressive group. Mean platelet volume count was similar in both groups. The N/L ratio was significantly higher in the progressive group (5.0 +/- 5.1 vs 3.2 +/- 3; P = .001). In multivariate analysis, the N/L ratio was significantly related with progression (relative risk [RR]: 2.267, 95% CI: 1.068-4.815, P = .03). Progression rate was significantly high in patients with high N/L ratio (39% vs 56%). Our results suggest that the N/L ratio is a predictor of progression of atherosclerosis

The assessment of atherosclerosis on vascular structures in patients with acute coronary syndrome

Introduction: Endothelial dysfunction plays a crucial role in the process of atherosclerotic diseases and has been accepted as an early stage of atherosclerosis. Carotid intima-media-thickness (CIMT) and flow-mediated-dilatation (FMD) of the brachial artery have been recommended as noninvasive methods to assess endothelial structure and function. Angiographic properties of patients with acute coronary syndrome (ACS) are closely associated with cardiovascular events. In this study, we investigated the relation of atherosclerotic properties of coronary, brachial and carotid arteries with CIMT, FMD and coronary angiography in patients with ACS. Methods: We enrolled 133 patients who were diagnosed with ACS into this study. Exclusion criteria were known coronary artery disease, diabetes mellitus and hypertension. Coronary angiography, CIMT and FMD were measured in all patients. The numbers of major stenotic coronary vessels with ?50% or ?70% were defined as diseased vessel. Gensini score was used to evaluate the severity of atherosclerosis. Morphologic properties of stenotic lesion were defined. Cutoff levels were 7% for FMD and 0.9 mm for CIMT. Results: Mean age was 59.7±11.8 years. FMD, CIMT and Gensini score were 8.3±5.9%, 0.80±0.19 mm and 7.8±3.5, respectively. Only 44% of patients with ACS had impaired FMD. Gensini score, number of diseased vessels and number of critical lesions were higher in patients with impaired FMD. (Gensini: 8.7±3.6 vs. 7.0±3.1, p=0.009, diseased vessels: 2.7±0.4 vs. 2.3±0.7, p < 0.0001, critical lesions: 3.0±2.1 vs. 2.2±1.4, p=0.02). Increased CIMT was found in only 33% of patients. Gensini score and number of diseased vessels were significantly higher in patients with increased CIMT. Significant but weak correlations were found between CIMT, FMD and angiographic severity of coronary atherosclerosis. Angiographic properties and lesion morphology were similar between CIMT and FMD groups. Conclusion: There appears to be a relationship between CIMT, FMD and severity of coronary atherosclerosis in patients with ACS. However, in patients with ACS, morphologic properties of stenotic lesions are not associated with CIMT and FMD in brachial artery

The assessment of atherosclerosis on vascular structures in patients with acute coronary syndrome

Introduction: Endothelial dysfunction plays a crucial role in the process of atherosclerotic diseases and has been accepted as an early stage of atherosclerosis. Carotid intimamedia- thickness (CIMT) and flow-mediated-dilatation (FMD) of the brachial artery have been recommended as noninvasive methods to assess endothelial structure and function. Angiographic properties of patients with acute coronary syndrome (ACS) are closely associated with cardiovascular events. In this study, we investigated the relation of atherosclerotic properties of coronary, brachial and carotid arteries with CIMT, FMD and coronary angiography in patients with ACS