Analysis of Gene expression in soybean (Glycine max) roots in response to the root knot nematode Meloidogyne incognita using microarrays and KEGG pathways

<p>Abstract</p> <p>Background</p> <p>Root-knot nematodes are sedentary endoparasites that can infect more than 3000 plant species. Root-knot nematodes cause an estimated $100 billion annual loss worldwide. For successful establishment of the root-knot nematode in its host plant, it causes dramatic morphological and physiological changes in plant cells. The expression of some plant genes is altered by the nematode as it establishes its feeding site.</p> <p>Results</p> <p>We examined the expression of soybean (<it>Glycine max</it>) genes in galls formed in roots by the root-knot nematode, <it>Meloidogyne incognita</it>, 12 days and 10 weeks after infection to understand the effects of infection of roots by <it>M. incognita</it>. Gene expression was monitored using the Affymetrix Soybean GeneChip containing 37,500 <it>G. max </it>probe sets. Gene expression patterns were integrated with biochemical pathways from the Kyoto Encyclopedia of Genes and Genomes using PAICE software. Genes encoding enzymes involved in carbohydrate and cell wall metabolism, cell cycle control and plant defense were altered.</p> <p>Conclusions</p> <p>A number of different soybean genes were identified that were differentially expressed which provided insights into the interaction between <it>M. incognita </it>and soybean and into the formation and maintenance of giant cells. Some of these genes may be candidates for broadening plants resistance to root-knot nematode through over-expression or silencing and require further examination.</p

Analysis of large versus small dogs reveals three genes on the canine X chromosome associated with body weight, muscling and back fat thickness

International audienceDomestic dog breeds display significant diversity in both body mass and skeletal size, resulting from intensive selective pressure during the formation and maintenance of modern breeds. While previous studies focused on the identification of alleles that contribute to small skeletal size, little is known about the underlying genetics controlling large size. We first performed a genome-wide association study (GWAS) using the Illumina Canine HD 170,000 single nucleotide polymorphism (SNP) array which compared 165 large-breed dogs from 19 breeds (defined as having a Standard Breed Weight (SBW) >41 kg [90 lb]) to 690 dogs from 69 small breeds (SBW ≤41 kg). We identified two loci on the canine X chromosome that were strongly associated with large body size at 82–84 megabases (Mb) and 101–104 Mb. Analyses of whole genome sequencing (WGS) data from 163 dogs revealed two indels in the Insulin Receptor Substrate 4 (IRS4) gene at 82.2 Mb and two additional mutations, one SNP and one deletion of a single codon, in Immunoglobulin Superfamily member 1 gene (IGSF1) at 102.3 Mb. IRS4 and IGSF1 are members of the GH/IGF1 and thyroid pathways whose roles include determination of body size. We also found one highly associated SNP in the 5’UTR of Acyl-CoA Synthetase Long-chain family member 4 (ACSL4) at 82.9 Mb, a gene which controls the traits of muscling and back fat thickness. We show by analysis of sequencing data from 26 wolves and 959 dogs representing 102 domestic dog breeds that skeletal size and body mass in large dog breeds are strongly associated with variants within IRS4, ACSL4 and IGSF1

power, envelope, OFDM,

code, Golay, complementary, sequence, pair, set Practical communications engineering is continuously producing problems in interest to the coding theory community. A recent example is the power-control problem in Orthogonal Frequency Division Multiplexing (OFDM). We report recent work which gives a mathematical framework for generating solutions to this notorious problem that are suited to low-cost wireless applications. The key result is a connection between Golay complementary sequences and Reed-Muller codes. The former are almost ideal for OFDM transmissions because they have a very low peak-to-mean envelope power ratio (PMEPR), while the latter have efficient encoding and decoding algorithms and good error correction capability. This result is then generalised in two ways. Firstly we study polyphase Golay sequences, motivating the introduction of non-binary generalisations of the Reed-Muller codes. Secondly we consider Golay complementary sets, where the results can be presented most naturally in the language of graph theory. The practical impact is a flexible family of OFDM codes which combine low PMEPR with good error correction capability. However, the interaction between theory and practice is a two-way process: the application motivates further study of a fertile interplay between coding theory, graph theory and sequence design. We include a list of open problems which we hope will stimulate further research in this area

Intelligent OFDM Telecommunication Systems Based on Many-Parameter Complex or Quaternion Fourier Transforms

In this paper, we propose novel Intelligent quaternion OFDM-telecommunication systems based on many-parameter complex and quaternion transform (MPFT). The new systems use inverse MPFT (IMPFT) for modulation at the transmitter and direct MPFT (DMPFT) for demodulation at the receiver. The purpose of employing the MPFT is to improve: (1) the PHY-LS of wireless transmissions against to the wide-band anti-jamming and anti-eavesdropping communication; (2) the bit error rate (BER) performance with respect to the conventional OFDM-TCS; (3) the peak to average power ratio (PAPR). Each MPFT depends on finite set of independent parameters (angles). When parameters are changed, many-parametric transform is also changed taking form of different quaternion orthogonal transforms. For this reason, the concrete values of parameters are specific “key” for entry into OFDM-TCS. Vector of parameters belong to multi-dimension torus space. Scanning of this space for find out the “key” (the concrete values of parameters) is hard problem. © 2020, The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG.Russian Foundation for Basic Researc