1,672 research outputs found
Emission lines of Fe X in active region spectra obtained with the Solar Extreme-ultraviolet Research Telescope and Spectrograph
Fully relativistic calculations of radiative rates and electron impact
excitation cross sections for Fe X are used to derive theoretical emission-line
ratios involving transitions in the 174-366 A wavelength range. A comparison of
these with solar active region observations obtained during the 1989 and 1995
flights of the Solar Extreme-ultraviolet Research Telescope and Spectrograph
(SERTS) reveals generally very good agreement between theory and experiment.
Several Fe X emission features are detected for the first time in SERTS
spectra, while the transition at 195.32 A is identified for the first time (to
our knowledge) in an astronomical source. The most useful Fe X electron density
diagnostic line ratios are assessed to be 175.27/174.53 and 175.27/177.24,
which both involve lines close in wavelength and free from blends, vary by
factors of 13 between Ne = 1E8 and 1E13 cm-3, and yet show little temperature
sensitivity. Should these lines not be available, then the 257.25/345.74 ratio
may be employed to determine Ne, although this requires an accurate evaluation
of the instrument intensity calibration over a relatively large wavelength
range. However, if the weak 324.73 A line of Fe X is reliably detected, the use
of 324.73/345.74 or 257.25/324.73 is recommended over 257.25/345.74.Comment: 11 pages, 10 figures, MNRAS in pres
Fe XI emission lines in a high resolution extreme ultraviolet spectrum obtained by SERTS
New calculations of radiative rates and electron impact excitation cross
sections for Fe XI are used to derive emission line intensity ratios involving
3s^23p^4 - 3s^23p^33d transitions in the 180-223 A wavelength range. These
ratios are subsequently compared with observations of a solar active region,
obtained during the 1995 flight Solar EUV Research Telescope and Spectrograph
(SERTS). The version of SERTS flown in 1995 incorporated a multilayer grating
that enhanced the instrumental sensitivity for features in the 170 - 225 A
wavelength range, observed in second-order between 340 and 450 A. This
enhancement led to the detection of many emission lines not seen on previous
SERTS flights, which were measured with the highest spectral resolution (0.03
A) ever achieved for spatially resolved active region spectra in this
wavelength range. However, even at this high spectral resolution, several of
the Fe XI lines are found to be blended, although the sources of the blends are
identified in the majority of cases. The most useful Fe XI electron density
diagnostic line intensity ratio is I(184.80 A)/I(188.21 A). This ratio involves
lines close in wavelength and free from blends, and which varies by a factor of
11.7 between N_e = 10^9 and 10^11 cm^-3, yet shows little temperature
sensitivity. An unknown line in the SERTS spectrum at 189.00 A is found to be
due to Fe XI, the first time (to our knowledge) this feature has been
identified in the solar spectrum. Similarly, there are new identifications of
the Fe XI 192.88, 198.56 and 202.42 A features, although the latter two are
blended with S VIII/Fe XII and Fe XIII, respectively.Comment: 21 pages, 9 gigures, accepted for publication in the Astrophysical
Journa
The critical dimension for a 4th order problem with singular nonlinearity
We study the regularity of the extremal solution of the semilinear biharmonic
equation \bi u=\f{\lambda}{(1-u)^2}, which models a simple
Micro-Electromechanical System (MEMS) device on a ball B\subset\IR^N, under
Dirichlet boundary conditions on . We complete
here the results of F.H. Lin and Y.S. Yang \cite{LY} regarding the
identification of a "pull-in voltage" \la^*>0 such that a stable classical
solution u_\la with 0 exists for \la\in (0,\la^*), while there is
none of any kind when \la>\la^*. Our main result asserts that the extremal
solution is regular provided while is singular () for , in which case
on the unit ball, where
and .Comment: 19 pages. This paper completes and replaces a paper (with a similar
title) which appeared in arXiv:0810.5380. Updated versions --if any-- of this
author's papers can be downloaded at this http://www.birs.ca/~nassif
Magnetic Anisotropy of a Single Cobalt Nanoparticle
Using a new microSQUID set-up, we investigate magnetic anisotropy in a single
1000-atoms cobalt cluster. This system opens new fields in the characterization
and the understanding of the origin of magnetic anisotropy in such
nanoparticles. For this purpose, we report three-dimensional switching field
measurements performed on a 3 nm cobalt cluster embedded in a niobium matrix.
We are able to separate the different magnetic anisotropy contributions and
evidence the dominating role of the cluster surface.Comment: 4 pages, 8 figure
KAUFMANN PURCELL, Susan & Françoise SIMON(dir.). Europe and Latin America in the World Economy. Boulder, Lynne Rienner Publishers, 1995, 215 p.
INTRODUCTION: Extracellular vesicles (EVs) are shed from cells and carry markers of the parent cells. Vesicles derived from cancer cells reach the bloodstream and locally influence important physiological processes. It has been previously shown that procoagulant vesicles are circulating in patients’ fluids. These EVs are therefore considered as promising biomarkers for the thrombotic risk. Because of their small size, classical methods such as flow cytometry suffer from limitation for their characterisation. Atomic force microscopy (AFM) has been proposed as a promising complementary method for the characterisation of EVs. OBJECTIVES: The objectives of this study are: (a) to develop and validate AFM with specific antibodies (anti-TF) and (b) to compare air and liquid modes for EVs’ size and number determination as potential biomarkers of the prothrombotic risk. METHODS: AFM multimode nanoscope III was used for air tapping mode (TM). AFM catalyst was used for liquid Peak Force Tapping (PFT) mode. Vesicles are generated according to Davila et al.'s protocol. Substrates are coated with various concentrations of antibodies, thanks to ethanolamine and glutaraldehyde. RESULTS: Vesicles were immobilised on antibody-coated surfaces to select tissue factor (TF)-positive vesicles. The size range of vesicles observed in liquid PFT mode is 6–10 times higher than in air mode. This corresponds to the data found in the literature. CONCLUSION: We recommend liquid PFT mode to analyse vesicles on 5 µg/ml antibody-coated substrates
Emission lines of Fe XI in the 257--407 A wavelength region observed in solar spectra from EIS/Hinode and SERTS
Theoretical emission-line ratios involving Fe XI transitions in the 257-407 A
wavelength range are derived using fully relativistic calculations of radiative
rates and electron impact excitation cross sections. These are subsequently
compared with both long wavelength channel Extreme-Ultraviolet Imaging
Spectrometer (EIS) spectra from the Hinode satellite (covering 245-291 A), and
first-order observations (235-449 A) obtained by the Solar Extreme-ultraviolet
Research Telescope and Spectrograph (SERTS). The 266.39, 266.60 and 276.36 A
lines of Fe XI are detected in two EIS spectra, confirming earlier
identifications of these features, and 276.36 A is found to provide an electron
density diagnostic when ratioed against the 257.55 A transition. Agreement
between theory and observation is found to be generally good for the SERTS data
sets, with discrepancies normally being due to known line blends, while the
257.55 A feature is detected for the first time in SERTS spectra. The most
useful Fe XI electron density diagnostic is found to be the 308.54/352.67
intensity ratio, which varies by a factor of 8.4 between N_e = 10^8 and 10^11
cm^-3, while showing little temperature sensitivity. However, the 349.04/352.67
ratio potentially provides a superior diagnostic, as it involves lines which
are closer in wavelength, and varies by a factor of 14.7 between N_e = 10^8 and
10^11 cm^-3. Unfortunately, the 349.04 A line is relatively weak, and also
blended with the second-order Fe X 174.52 A feature, unless the first-order
instrument response is enhanced.Comment: 9 pages, 5 figures, 13 tables; MNRAS in pres
Bioaffinity sensor based on nanoarchitectonic films: control of the specific adsorption of proteins through the dual role of an ethylene oxide spacer.
The identification and quantification of biomarkers or proteins is a real challenge in allowing the early detection of diseases. The functionalization of the biosensor surface has to be properly designed to prevent nonspecific interactions and to detect the biomolecule of interest specifically. A multilayered nanoarchitecture, based on polyelectrolyte multilayers (PEM) and the sequential immobilization of streptavidin and a biotinylated antibody, was elaborated as a promising platform for the label-free sensing of targeted proteins. We choose ovalbumin as an example. Thanks to the versatility of PEM films, the platform was built on two types of sensor surface and was evaluated using both optical- and viscoelastic-based techniques, namely, optical waveguide lightmode spectroscopy and the quartz crystal microbalance, respectively. A library of biotinylated poly(acrylic acids) (PAAs) was synthesized by grafting biotin moieties at different grafting ratios (GR). The biotin moieties were linked to the PAA chains through ethylene oxide (EO) spacers of different lengths. The adsorption of the PAA-EOn-biotin (GR) layer on a PEM precursor film allows tuning the surface density in biotin and thus the streptavidin adsorption mainly through the grafting ratio. The nonspecific adsorption of serum was reduced and even suppressed depending on the length of the EO arms. We showed that to obtain an antifouling polyelectrolyte the grafting of EO9 or EO19 chains at 25% in GR is sufficient. Thus, the spacer has a dual role: ensuring the antifouling property and allowing the accessibility of biotin moieties. Finally, an optimized platform based on the PAA-EO9-biotin (25%)/streptavidin/biotinylated-antibody architecture was built and demonstrated promising performance as interface architecture for bioaffinity sensing of a targeted protein, in our case, ovalbumin.journal articleresearch support, non-u.s. gov't2013 Jun 182013 02 11importe
Safety, tumor trafficking and immunogenicity of chimeric antigen receptor (CAR)-T cells specific for TAG-72 in colorectal cancer.
BackgroundT cells engineered to express chimeric antigen receptors (CARs) have established efficacy in the treatment of B-cell malignancies, but their relevance in solid tumors remains undefined. Here we report results of the first human trials of CAR-T cells in the treatment of solid tumors performed in the 1990s.MethodsPatients with metastatic colorectal cancer (CRC) were treated in two phase 1 trials with first-generation retroviral transduced CAR-T cells targeting tumor-associated glycoprotein (TAG)-72 and including a CD3-zeta intracellular signaling domain (CART72 cells). In trial C-9701 and C-9702, CART72 cells were administered in escalating doses up to 1010 total cells; in trial C-9701 CART72 cells were administered by intravenous infusion. In trial C-9702, CART72 cells were administered via direct hepatic artery infusion in patients with colorectal liver metastases. In both trials, a brief course of interferon-alpha (IFN-α) was given with each CART72 infusion to upregulate expression of TAG-72.ResultsFourteen patients were enrolled in C-9701 and nine in C-9702. CART72 manufacturing success rate was 100% with an average transduction efficiency of 38%. Ten patients were treated in CC-9701 and 6 in CC-9702. Symptoms consistent with low-grade, cytokine release syndrome were observed in both trials without clear evidence of on target/off tumor toxicity. Detectable, but mostly short-term (≤14 weeks), persistence of CART72 cells was observed in blood; one patient had CART72 cells detectable at 48 weeks. Trafficking to tumor tissues was confirmed in a tumor biopsy from one of three patients. A subset of patients had 111Indium-labeled CART72 cells injected, and trafficking could be detected to liver, but T cells appeared largely excluded from large metastatic deposits. Tumor biomarkers carcinoembryonic antigen (CEA) and TAG-72 were measured in serum; there was a precipitous decline of TAG-72, but not CEA, in some patients due to induction of an interfering antibody to the TAG-72 binding domain of humanized CC49, reflecting an anti-CAR immune response. No radiologic tumor responses were observed.ConclusionThese findings demonstrate the relative safety of CART72 cells. The limited persistence supports the incorporation of co-stimulatory domains in the CAR design and the use of fully human CAR constructs to mitigate immunogenicity
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