Sodium affects the sperm motility in the European eel

[EN] The role of seminal plasma sodium and activation media sodium on sperm motility was examined by selectively removing the element from these two media, in European eel sperm. Sperm size (sperm head area) was also measured using an ASMA (Automated Sperm Morphometry Analyses) system, in the different conditions. Intracellular sodium [Na+](i) was quantitatively analyzed by first time in the spermatozoa from a marine fish species. Measurement of [Na+](i) was done before and after motility activation, by Flow Cytometry, using CoroNa Green AM as a dye. Sperm motility activation induced an increase in [Na+](i) from 96.72 mM in quiescent stage to 152.21 mM post-activation in seawater. A significant decrease in sperm head area was observed post activation in seawater. There was a notable reduction in sperm motility when sodium was removed from the seminal plasma, but not when it was removed from the activation media. Sodium removal was also linked to a significant reduction in sperm head area in comparison to the controls. Our results indicate that the presence of the ion Na+ in the seminal plasma (or in the extender medium) is necessary for the preservation of sperm motility in European eel, probably because it plays a role in maintaining an appropriate sperm cell volume in the quiescent stage of the spermatozoa. (C) 2016 Elsevier Inc All rights reserved.Funded from the SPERMOT project (Spanish Ministry of Science and Innovation, MICINN; AGL2010-16009). M.C. Vilchez has a predoctoral grant from UPV PAID Program (2011-S2-02-6521), Marina Morini has a predoctoral grant from Generalitat Valenciana (Programa Grisolia, GRISOLIA/2012/006), Victor Gallego has a postdoctoral contract from UPV (PAID-10-14), and David S. Penaranda was supported by MICINN (PTA2011-4948-I) and UPV (PTA2011-4948-I). Grants to attend meetings were received from COST Office (Food and Agriculture COST Action FA1205: AQUAGAMETE).Vilchez Olivencia, MC.; Morini, M.; Peñaranda, D.; Gallego Albiach, V.; Asturiano Nemesio, JF.; Pérez Igualada, LM. (2016). Sodium affects the sperm motility in the European eel. Comparative Biochemistry and Physiology - B: Comparative Biochemistry. 198:51-58. https://doi.org/10.1016/j.cbpa.2016.04.008S515819

Role of potassium and pH on the initiation of sperm motility in the European eel

[EN] The role of potassium from the seminal plasma and/or the activation media was examined by selectively removing from this media, and by testing the use of channel inhibitors and a K-ionophore. Sperm motility was measured using a CASA system, intracellular K+ and pH were measured by flow cytometry, and sperm head area was measured by ASMA: Automated Sperm Morphometry Analyses. Sperm motility was notably inhibited by the removal of K+ from the seminal plasma and by treatment with the K+ ionophore valinomycin. This therefore indicates that a reduction of K+ levels in the quiescent stage inhibits further motility. The normal decrease in sperm head area induced by seawater activation was altered by the removal of K+ from the seminal plasma, and an increase in the pH; in the quiescent stage was also induced. Intracellular pH (pH;) was quantitatively measured for the first time in European eel spermatozoa, being 7.2 in the quiescent stage and 7.1 post-activation. Intracellular and external pH levels influenced sperm motility both in the quiescent stage and at activation. The alkalinization of the pH; (by NH4Cl) inhibited sperm motility activation, while acidification (by Na-acetate) did not have any effect. Our results indicate that a pH gradient between the sperm cell and the seminal plasma is necessary for sperm motility activation. The presence of the ion K+ in the seminal plasma (or in the extender medium) is necessary in order to maintain sperm volume, intracellular pH and sperm motility.Funded from the SPERMOT project (Spanish Ministry of Science and Innovation, MINECO AGL2010-16009). M.C. Vilchez has a predoctoral grant from UPV PAID Subprogramme 2 (2011-S2-02-6521), Marina Morini has a predoctoral grant from Generalitat Valenciana (Programa Grisolia, GRISOLIA/2012/006), Victor Gallego has a postdoctoral contract from UPV (PAID-10-14), and David S. Penaranda was supported by MICINN (PTA2011-4948-1) and UPV (PTA2011-4948-I). Grants to attend meetings were received from COST Office (AQUAGAMETE COST Action: FA1205).Vilchez Olivencia, MC.; Morini, M.; Peñaranda, D.; Gallego Albiach, V.; Asturiano Nemesio, JF.; Pérez Igualada, LM. (2017). Role of potassium and pH on the initiation of sperm motility in the European eel. Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology. 203:210-219. https://doi.org/10.1016/j.cbpa.2016.09.024S21021920

The expression of nuclear and membrane estrogen receptors in the European eel throughout spermatogenesis

[EN] Estradiol (E-2) can bind to nuclear estrogen receptors (ESR) or membrane estrogen receptors (GPER). While mammals possess two nuclear ESRs and one membrane GPER, the European eel, like most other teleosts, has three nuclear ESRs and two membrane GPERs, as the result of a teleost specific genome duplication. In the current study, the expression of the three nuclear ESRs (ESR1, ESR2a and ESR2b) and the two membrane GPERs (GPERa and GPERb) in the brain-pituitary-gonad (BPG) axis of the European eel was measured, throughout spermatogenesis. The eels were first transferred from freshwater (FW) to seawater (SW), inducing parallel increases in E2 plasma levels and the expression of ESRs. This indicates that salinity has a stimulatory effect on the E-2 signalling pathway along the BPG axis. Stimulation of sexual maturation by weekly injections of human chorionic gonadotropin (hCG) induced a progressive decrease in E-2 plasma levels, and different patterns of expression of ESRs and GPERs in the BPG axis. The expression of nuclear ESRs increased in some parts of the brain, suggesting a possible upregulation due to a local production of E-2. In the testis, the highest expression levels of the nuclear ESRs were observed at the beginning of spermatogenesis, possibly mediating the role of E2 as spermatogonia renewal factor, followed by a sharply decrease in the expression of ESRs. Conversely, there was a marked increase observed in the expression of both membrane GPERs throughout spermatogenesis, suggesting they play a major role in the final stages of spermatogenesis.Funded by the Spanish Ministry of Science and Innovation (REPRO-TEMP project; AGL2013-41646-R) and IMPRESS (Marie Sklodowska-Curie Actions; Grant agreement no: 642893). M.C. Vilchez has a predoctoral grant from UPV PAID Programme (2011-S2-02-6521), M. Morini has a predoctoral grant from Generalitat Valenciana (Programa Grisolia). D.S. Penaranda was supported by MICINN and UPV (PTA2011-4948-1).Morini, M.; Peñaranda, D.; Vilchez Olivencia, MC.; Tveiten, H.; Lafont, A.; Dufour, S.; Pérez Igualada, LM.... (2017). The expression of nuclear and membrane estrogen receptors in the European eel throughout spermatogenesis. Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology. 203:91-99. doi:10.1016/j.cbpa.2016.08.020S919920

Mejora de las competencias gracias a la implantación de aprendizaje basado en proyectos en acuicultura

En el presente trabajo se muestra la experiencia de la aplicación del Aprendizaje Basada en proyectos (ABP) en la asignatura “Diseño y Gestión de instalaciones Piscícolas” de la titulación del Máster interuniversitario de Acuicultura de la Universitat Politécnica de València. El ABP consistió en el desarrollo de un proyecto de una piscifactoría, en el cual los alumnos debían integrar los conocimientos adquiridos en las asignaturas previas, permitiendo una visión más multidisciplinar de la asignatura y mejor adquisición de las competencias. Tanto desde el punto de vista del alumnado como del profesor, la implantación del ABP permitió un mayor desarrollo de las competencias, siendo la metodología docente valorada positivamente. Además, se mejoró la participación del alumnado en las actividades propuestas, dando como resultado una mejor calificando académica final. No obstante, se deben de mejorar algunos aspectos como es la planificación del tiempo y consolidar relaciones de integración o la interacción entre diferentes disciplinas/asignaturas de manera que tengan una mejor integración en el proyecto

Aprendizaje basado en proyectos: una propuesta eficaz para el desarrollo de las competencias en el master de Acuicultura

[ES] En el presente trabajo se muestra una experiencia de la aplicación del Aprendizaje Basada en proyectos en la titulación del Master interuniversitario de Acuicultura de la Universitat Politécnica de València. El ABPr se centró en el desarrollo de un proyecto de una piscifactoría, en el cual los alumnos pudieran adquirir una visión en conjunto de las asignaturas impartidas en el máster, fomentando la conexión entre ellas, reforzando, por tanto, el carácter interdisciplinar del proyecto. Para evaluar la metodología se realizó cuestionario cuantitativo y otro cualitativo con el objetivo de conocer las opiniones de los alumnos. Los alumnos han valorado positivamente la metodología, destacando la importancia del proyecto desarrollado para su formación profesional, así como también se valoró sustancialmente que el trabajo desarrollado en grupo cumplió sus expectativas en cuanto a la labor de los docentes. Aunque no se observaron valoraciones negativas de la metodología desarrollada, se deben de mejorar algunos aspectos como la planificación del tiempo y consolidar relaciones de integración o la interacción entre diferentes disciplinas/asignaturas.[EN] This work shows an of Project Based Learning experience on Interuniversity Master of Aquaculture degree at the Universitat Politécnica de València. The ABPr focused on the development of a fish farm project, in which the students could acquire a combined vision of the subjects taught during the master's degree, promoting the connection between subjects, thus reinforcing the interdisciplinary character of the project. To evaluate the methodology, a quantitative and a qualitative survey was conducted with the aim to know the students opinions. The students have positively valued the methodology, highlighting the importance of the project developed for their professional training, as well as the fact that the project carried out as teamwork met their expectations regarding the teachers´work. Although no negative evaluations about the developed methodology were observed, some aspects such as time scheduling, and the integration of additional subjects/ disciplines should be improved.Martínez-Llorens, S.; Jauralde García, I.; S. Peñaranda, D.; Tomás-Vidal, A.; Jover Cerdá, M. (2021). Aprendizaje basado en proyectos: una propuesta eficaz para el desarrollo de las competencias en el master de Acuicultura. En IN-RED 2020: VI Congreso de Innovación Educativa y Docencia en Red. Editorial Universitat Politècnica de València. 208-2017. https://doi.org/10.4995/INRED2020.2020.11988OCS208201

Blending learning: Una nueva forma de enfocar las sesiones prácticas

Las competencias son elementos muy apreciados por los empleadores, en especial las relacionadas con el pensamiento práctico. Esta realidad es más evidente en las sesiones prácticas de laboratorio, donde los alumnos deben aplicar conocimientos teóricos a casos prácticos. En el actual trabajo se pretende implantar la metodología docente “Blended learning” que combina docencia presencial con on line, con el fin de favorecer la adquisición de la competencia transversal “Aplicación y pensamiento práctico”, así como una mayor interiorización de los conceptos aprendidos. La metodología docente se implantó en alumnos de grado, en concreto en las sesiones de laboratorio de la asignatura: Fisiología Animal y Humana (11109) del segundo curso del Grado en Biotecnología de la Universitat Politècnica de València. Los resultados obtenidos mostraron que la implantación de la metodología docente permitió desarrollar el pensamiento práctico, ya que la nota media categórica fue de “A: Excelente”. Además, se observó una mejora en la calificación de la nota del examen teórico-práctico, lo que indica una mejor interiorización de los conceptos impartidos. Por tanto, se puede concluir que la metodología “Blended learning” ha sido una metodología eficaz para no sólo mejorar la adquisición de la competencia transversal, sino también para la calificación académica

SUSY-QCD decoupling properties in H+ -> t \bar b decay

The SUSY-QCD radiative corrections to the \Gamma (H+ -> t \bar b) partial decay width are analyzed within the Minimal Supersymmetric Standard Model at the one-loop level, {\mathcal O}(\alpha_s), and in the decoupling limit. We present the analytical expressions of these corrections in the large SUSY masses limit and study the decoupling behaviour of these corrections in various limiting cases. We find that if the SUSY mass parameters are large and of the same order, the one loop SUSY-QCD corrections {\it do not decouple}. The non-decoupling contribution is enhanced by \tan \beta and therefore large corrections are expected in the large \tan \beta limit. In contrast, we also find that the SUSY-QCD corrections decouple if the masses of either the squarks or the gluinos are separately taken large.Comment: LaTeX, 33 pages, 7 figure included. Uses cite.st

Effect of the probiotic Lactobacillus rhamnosus on the expression of genes involved in European eel spermatogenesis

[EN] Positive effects of probiotics on fish reproduction have been reported in several species. In the present study, 40 male European eels were weekly treated with recombinant hCG for 9 weeks and with three different concentrations (10(3), 10(5), and 10(6) CFU/mL) of probiotic Lactobacillus rhamnosus IMC 501 (Sinbyotec, Italy). The probiotics were daily added to the water from the sixth week of the hCG treatment. Males from the treated and control groups were sacrificed after 1, 2, and 3 weeks of probiotic treatment (seventh ninth weeks of hCG treatment); at this point, sperm and testis samples were also collected. Sperm volume was estimated, and motility was analyzed by computer-assisted sperm analysis software. Alternations in transcription of specific genes involved in reproductive process such as activin, androgen receptors alpha and beta (ar alpha and ar beta), progesterone receptor 1 (pr1), bone morphogenetic protein 15 (bmp15), and FSH receptor (fshr) were analyzed in the testis. After 2 weeks of probiotic treatment, sperm production and sperm motility parameters (percentage of motile cells and percentage of straight-swimming spermatozoa) were increased in the European eel treated with 105 CFU/mL compared to controls or to the other probiotic doses. These changes were associated with increases in messenger RNA expression of activin, ar alpha, ar beta, pr1, and fshr. Conversely, after 3 weeks, activin and pr1 expression decreased. No significant changes were observed on bmp15 expression throughout the duration of the treatment with 10(5) CFU/mL dose. The lowest and highest probiotic dose (10(3) and 10(6) CFU/mL, respectively) inhibited the transcription of all genes along all the experiment, except for ar alpha and ar beta after 1 week of probiotic treatment when compared to controls. The changes observed by transcriptomic analysis and the sperm parameters suggest that a treatment with L rhamnosus at 10(5) CFU/mL for 2 weeks could improve spermatogenesis process in Anguilla anguilla. (C) 2015 Elsevier Inc. All rights reserved.This study was funded by the European Community’s 7th FP (grant agreement no. 245257, PRO-EEL) and COST Office (Food and Agriculture COST Action FA1205: AQUAGAMETE) Victor Gallego and M. Carmen Vilchez have predoctoral grants from MINISTERIO DE ECONOMIA Y COMPETITIVIDAD (BES-2009-020310) and UNIVERSIDAD POLITECNICA DE VALENCIA PAID Program (2011-S2-02-6521), respectively. Fondo Ateneo 2012 to Oliana Carnevali.Vilchez Olivencia, MC.; Santangeli, S.; Maradonna, F.; Gioacchini, G.; Verdenelli, C.; Gallego Albiach, V.; Peñaranda, D.... (2015). Effect of the probiotic Lactobacillus rhamnosus on the expression of genes involved in European eel spermatogenesis. Theriogenology. 84(8):1321-1331. https://doi.org/10.1016/j.theriogenology.2015.07.011S1321133184

Transcriptome profiling of rabbit parthenogenetic blastocysts developed under in vivo conditions

Parthenogenetic embryos are one attractive alternative as a source of embryonic stem cells, although many aspects related to the biology of parthenogenetic embryos and parthenogenetically derived cell lines still need to be elucidated. The present work was conducted to investigate the gene expression profile of rabbit parthenote embryos cultured under in vivo conditions using microarray analysis. Transcriptomic profiles indicate 2541 differentially expressed genes between parthenotes and normal in vivo fertilised blastocysts, of which 76 genes were upregulated and 16 genes downregulated in in vivo cultured parthenote blastocyst, using 3 fold-changes as a cut-off. While differentially upregulated expressed genes are related to transport and protein metabolic process, downregulated expressed genes are related to DNA and RNA binding. Using microarray data, 6 imprinted genes were identified as conserved among rabbits, humans and mice: GRB10, ATP10A, ZNF215, NDN, IMPACT and SFMBT2. We also found that 26 putative genes have at least one member of that gene family imprinted in other species. These data strengthen the view that a large fraction of genes is differentially expressed between parthenogenetic and normal embryos cultured under the same conditions and offer a new approach to the identification of imprinted genes in rabbit. © 2012 Naturil-Alfonso et al.This work was supported by Generalitat Valenciana research programme (Prometeo 2009/125). Carmen Naturil was supported by Generalitat Valenciana research programme (Prometeo 2009/125). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Naturil Alfonso, C.; Saenz De Juano Ribes, MDLD.; Peñaranda, D.; Vicente Antón, JS.; Marco Jiménez, F. (2012). Transcriptome profiling of rabbit parthenogenetic blastocysts developed under in vivo conditions. PLoS ONE. 7(12):1-11. https://doi.org/10.1371/journal.pone.0051271S111712Harness, J. V., Turovets, N. A., Seiler, M. J., Nistor, G., Altun, G., Agapova, L. S., … Keirstead, H. S. (2011). Equivalence of Conventionally-Derived and Parthenote-Derived Human Embryonic Stem Cells. PLoS ONE, 6(1), e14499. doi:10.1371/journal.pone.0014499Lu, Z., Zhu, W., Yu, Y., Jin, D., Guan, Y., Yao, R., … Zhou, Q. (2010). Derivation and long-term culture of human parthenogenetic embryonic stem cells using human foreskin feeders. Journal of Assisted Reproduction and Genetics, 27(6), 285-291. doi:10.1007/s10815-010-9408-5Koh, C. J., Delo, D. M., Lee, J. W., Siddiqui, M. M., Lanza, R. P., Soker, S., … Atala, A. (2009). Parthenogenesis-derived multipotent stem cells adapted for tissue engineering applications. Methods, 47(2), 90-97. doi:10.1016/j.ymeth.2008.08.002Vrana, K. E., Hipp, J. D., Goss, A. M., McCool, B. A., Riddle, D. R., Walker, S. J., … Cibelli, J. B. (2003). Nonhuman primate parthenogenetic stem cells. Proceedings of the National Academy of Sciences, 100(Supplement 1), 11911-11916. doi:10.1073/pnas.2034195100Chen, Z., Liu, Z., Huang, J., Amano, T., Li, C., Cao, S., … Liu, L. (2009). Birth of Parthenote Mice Directly from Parthenogenetic Embryonic Stem Cells. Stem Cells, 27(9), 2136-2145. doi:10.1002/stem.158Sritanaudomchai, H., Ma, H., Clepper, L., Gokhale, S., Bogan, R., Hennebold, J., … Mitalipov, S. (2010). Discovery of a novel imprinted gene by transcriptional analysis of parthenogenetic embryonic stem cells. Human Reproduction, 25(8), 1927-1941. doi:10.1093/humrep/deq144Fang, Z. F., Gai, H., Huang, Y. Z., Li, S. G., Chen, X. J., Shi, J. J., … Sheng, H. Z. (2006). Rabbit embryonic stem cell lines derived from fertilized, parthenogenetic or somatic cell nuclear transfer embryos. Experimental Cell Research, 312(18), 3669-3682. doi:10.1016/j.yexcr.2006.08.013Wang, S., Tang, X., Niu, Y., Chen, H., Li, B., Li, T., … Ji, W. (2007). Generation and Characterization of Rabbit Embryonic Stem Cells. Stem Cells, 25(2), 481-489. doi:10.1634/stemcells.2006-0226Piedrahita, J. A., Anderson, G. B., & BonDurant, R. H. (1990). On the isolation of embryonic stem cells: Comparative behavior of murine, porcine and ovine embryos. Theriogenology, 34(5), 879-901. doi:10.1016/0093-691x(90)90559-cNaturil-Alfonso, C., Saenz-de-Juano, M. D., Peñaranda, D. S., Vicente, J. S., & Marco-Jiménez, F. (2011). Parthenogenic blastocysts cultured under in vivo conditions exhibit proliferation and differentiation expression genes similar to those of normal embryos. Animal Reproduction Science, 127(3-4), 222-228. doi:10.1016/j.anireprosci.2011.08.005Besenfelder, U., Strouhal, C., & Brem, G. (1998). A Method for Endoscopic Embryo Collection and Transfer in the Rabbit. Journal of Veterinary Medicine Series A, 45(1-10), 577-579. doi:10.1111/j.1439-0442.1998.tb00861.xMehaisen, G. M. K., Viudes-de-Castro, M. P., Vicente, J. S., & Lavara, R. (2006). In vitro and in vivo viability of vitrified and non-vitrified embryos derived from eCG and FSH treatment in rabbit does. Theriogenology, 65(7), 1279-1291. doi:10.1016/j.theriogenology.2005.08.007Bilodeau-Goeseels, S., & Schultz, G. A. (1997). Changes in Ribosomal Ribonucleic Acid Content Within in Vitro-produced Bovine Embryos1. Biology of Reproduction, 56(5), 1323-1329. doi:10.1095/biolreprod56.5.1323Conesa, A., Gotz, S., Garcia-Gomez, J. M., Terol, J., Talon, M., & Robles, M. (2005). Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research. Bioinformatics, 21(18), 3674-3676. doi:10.1093/bioinformatics/bti610Edgar, R. (2002). Gene Expression Omnibus: NCBI gene expression and hybridization array data repository. Nucleic Acids Research, 30(1), 207-210. doi:10.1093/nar/30.1.207Weltzien, F.-A., Pasqualini, C., Vernier, P., & Dufour, S. (2005). A quantitative real-time RT-PCR assay for European eel tyrosine hydroxylase. General and Comparative Endocrinology, 142(1-2), 134-142. doi:10.1016/j.ygcen.2004.12.019Llobat, L., Marco-Jiménez, F., Peñaranda, D., Saenz-de-Juano, M., & Vicente, J. (2011). Effect of Embryonic Genotype on Reference Gene Selection for RT-qPCR Normalization. Reproduction in Domestic Animals, 47(4), 629-634. doi:10.1111/j.1439-0531.2011.01934.xLiu, N., Enkemann, S. A., Liang, P., Hersmus, R., Zanazzi, C., Huang, J., … Liu, L. (2010). Genome-wide Gene Expression Profiling Reveals Aberrant MAPK and Wnt Signaling Pathways Associated with Early Parthenogenesis. Journal of Molecular Cell Biology, 2(6), 333-344. doi:10.1093/jmcb/mjq029Abdoon, A. S., Ghanem, N., Kandil, O. M., Gad, A., Schellander, K., & Tesfaye, D. (2012). cDNA microarray analysis of gene expression in parthenotes and in vitro produced buffalo embryos. Theriogenology, 77(6), 1240-1251. doi:10.1016/j.theriogenology.2011.11.004Labrecque, R., & Sirard, M.-A. (2011). Gene expression analysis of bovine blastocysts produced by parthenogenic activation or fertilisation. Reproduction, Fertility and Development, 23(4), 591. doi:10.1071/rd10243Rizos, D., Clemente, M., Bermejo-Alvarez, P., de La Fuente, J., Lonergan, P., & Gutiérrez-Adán, A. (2008). Consequences ofIn VitroCulture Conditions on Embryo Development and Quality. Reproduction in Domestic Animals, 43, 44-50. doi:10.1111/j.1439-0531.2008.01230.xLonergan, P., Rizos, D., Kanka, J., Nemcova, L., Mbaye, A., Kingston, M., … Boland, M. (2003). Temporal sensitivity of bovine embryos to culture environment after fertilization and the implications for blastocyst quality. Reproduction, 337-346. doi:10.1530/rep.0.1260337Memili, E., & First, N. L. (2000). Zygotic and embryonic gene expression in cow: a review of timing and mechanisms of early gene expression as compared with other species. Zygote, 8(1), 87-96. doi:10.1017/s0967199400000861Latham, K. E. (2001). Embryonic genome activation. Frontiers in Bioscience, 6(3), d748-759. doi:10.2741/a639Niemann, H., & Wrenzycki, C. (2000). Alterations of expression of developmentally important genes in preimplantation bovine embryos by in vitro culture conditions: Implications for subsequent development. Theriogenology, 53(1), 21-34. doi:10.1016/s0093-691x(99)00237-xCorcoran, D., Fair, T., Park, S., Rizos, D., Patel, O. V., Smith, G. W., … Lonergan, P. (2006). Suppressed expression of genes involved in transcription and translation in in vitro compared with in vivo cultured bovine embryos. Reproduction, 131(4), 651-660. doi:10.1530/rep.1.01015Morison, I. M., Ramsay, J. P., & Spencer, H. G. (2005). A census of mammalian imprinting. Trends in Genetics, 21(8), 457-465. doi:10.1016/j.tig.2005.06.008Bischoff, S. R., Tsai, S., Hardison, N., Motsinger-Reif, A. A., Freking, B. A., Nonneman, D., … Piedrahita, J. A. (2009). Characterization of Conserved and Nonconserved Imprinted Genes in Swine1. 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SUSY-QCD corrections to the MSSM h0bbˉh^0 b \bar b vertex in the decoupling limit

We analyze the supersymmetric (SUSY) QCD contribution to the $h^0 b \bar{b}$ coupling at one loop in the Minimal Supersymmetric Model (MSSM) in the decoupling limit. Analytic expressions in the large SUSY mass region are derived and the decoupling behavior of the corrections is examined in various limiting cases, where some or all of the SUSY mass parameters become large. We show that in the decoupling limit of large SUSY mass parameters and large CP-odd Higgs mass, the $h^0 b \bar b$ coupling approaches its Standard Model value at one loop. However, the onset of decoupling is delayed when $\tan\beta$ is large. In addition, the one-loop SUSY-QCD corrections decouple if the masses of either the bottom squarks or the gluino are separately taken large; although the approach to decoupling is significantly slower in the latter case.Comment: 27 pages, 7 figures and 1 table in LaTeX2e format; with a few minor changes and updated reference