Controlled cortical impact traumatic brain injury in 3xTg-AD mice causes acute intra-axonal amyloid-β accumulation and independently accelerates the development of tau abnormalities

Alzheimer\u27s disease (AD) is a neurodegenerative disorder characterized pathologically by progressive neuronal loss, extracellular plaques containing the amyloid-β (Aβ) peptides, and neurofibrillary tangles composed of hyperphosphorylated tau proteins. Aβ is thought to act upstream of tau, affecting its phosphorylation and therefore aggregation state. One of the major risk factors for AD is traumatic brain injury (TBI). Acute intra-axonal Aβ and diffuse extracellular plaques occur in ∼30% of human subjects after severe TBI. Intra-axonal accumulations of tau but not tangle-like pathologies have also been found in these patients. Whether and how these acute accumulations contribute to subsequent AD development is not known, and the interaction between Aβ and tau in the setting of TBI has not been investigated. Here, we report that controlled cortical impact TBI in 3xTg-AD mice resulted in intra-axonal Aβ accumulations and increased phospho-tau immunoreactivity at 24 h and up to 7 d after TBI. Given these findings, we investigated the relationship between Aβ and tau pathologies after trauma in this model by systemic treatment of Compound E to inhibit γ-secretase activity, a proteolytic process required for Aβ production. Compound E treatment successfully blocked posttraumatic Aβ accumulation in these injured mice at both time points. However, tau pathology was not affected. Our data support a causal role for TBI in acceleration of AD-related pathologies and suggest that TBI may independently affect Aβ and tau abnormalities. Future studies will be required to assess the behavioral and long-term neurodegenerative consequences of these pathologies

Effect of genotype on duodenal expression of nutrient transporter genes in dairy cows

peer-reviewedBackground Studies have shown clear differences between dairy breeds in their feed intake and production efficiencies. The duodenum is critical in the coordination of digestion and absorption of nutrients. This study examined gene transcript abundance of important classes of nutrient transporters in the duodenum of non lactating dairy cows of different feed efficiency potential, namely Holstein-Friesian (HF), Jersey (JE) and their F1 hybrid. Duodenal epithelial tissue was collected at slaughter and stored at -80°C. Total RNA was extracted from tissue and reverse transcribed to generate cDNA. Gene expression of the following transporters, namely nucleoside; amino acid; sugar; mineral; and lipid transporters was measured using quantitative real-time RT-PCR. Data were statistically analysed using mixed models ANOVA in SAS. Orthogonal contrasts were used to test for potential heterotic effects and spearman correlation coefficients calculated to determine potential associations amongst gene expression values and production efficiency variables. Results While there were no direct effects of genotype on expression values for any of the genes examined, there was evidence for a heterotic effect (P < 0.05) on ABCG8, in the form of increased expression in the F1 genotype compared to either of the two parent breeds. Additionally, a tendency for increased expression of the amino acid transporters, SLC3A1 (P = 0.072), SLC3A2 (P = 0.081) and SLC6A14 (P = 0.072) was also evident in the F1 genotype. A negative (P < 0.05) association was identified between the expression of the glucose transporter gene SLC5A1 and total lactational milk solids yield, corrected for body weight. Positive correlations (P < 0.05) were also observed between the expression values of genes involved in common transporter roles. Conclusion This study suggests that differences in the expression of sterol and amino acid transporters in the duodenum could contribute towards the documented differences in feed efficiency between HF, JE and their F1 hybrid. Furthermore, positive associations between the expression of genes involved in common transporter roles suggest that these may be co-regulated. The study identifies potential candidates for investigation of genetic variants regulating nutrient transport and absorption in the duodenum in dairy cows, which may be incorporated into future breeding programmes

THE TAXONOMIC STATUS OF THE WYOMING TOAD, BUFO BAXTERI PORTER

The population of toads in southeastern Wyoming named Bufo hemiophrys baxteri by Porter in 1968 is presumed to be extinct in nature, except perhaps for released, captive-bred specimens. It is sufficiently distinct in several respects, and sufficiently isolated geographically from its nearest rela- tive, B. h. hemiophrys, that it should be regarded as a distinct species, forming a superspecies group with B. hemiophrys

African gateways : measuring airline connectivity change for Africa's global urban networks in the 2003-2009 period

This paper studies the globalization of major African cities through their changing insertion in global airline networks. As such, the paper adds to a growing body of literature that analyzes the role of infrastructure in the formation of world-city networks. We draw on a rich data source that provides longitudinal airline booking data, which can be directly linked up to the evolution of inter-urban networks at the African and global scale. Our results indicate that Johannesburg remains the major gateway to Africa, but other regional centers and in particular Cairo, Lagos, Casablanca and Nairobi are rapidly complementing Johannesburg in this role. The globalization of African cities is related to rapid network growth on the African continent, but is outrun by fast growth in terms of non-African connections. Among the latter, connections to Asia and the Middle East are rapidly gaining importance. In the end, the paper argues that SouthSouth connections are crucial for an understanding of the contemporary globalization of African cities

FIELD TRIALS AS AN EXTENSION TECHNIQUE: THE CASE OF SWAZILAND

One potentially serious problem in evaluating the effectiveness of extension programs is that participants are not picked at random. Self-selection can be a problem, and it can be compounded if extension officials concentrate on the most progressive farms. This study explores the relationships between adoption of maize high-yielding varieties (HYVs) and participation in field trials intended to foster HYV usage, drawing on data from Swaziland. Results indicate that it is impossible to say if field trials had any effect on adoption. Participating farms used more HYVs, but this could have been due to self-selection or the government's selection process.Teaching/Communication/Extension/Profession,

PEPS as unique ground states of local Hamiltonians

In this paper we consider projected entangled pair states (PEPS) on arbitrary lattices. We construct local parent Hamiltonians for each PEPS and isolate a condition under which the state is the unique ground state of the Hamiltonian. This condition, verified by generic PEPS and examples like the AKLT model, is an injective relation between the boundary and the bulk of any local region. While it implies the existence of an energy gap in the 1D case we will show that in certain cases (e.g., on a 2D hexagonal lattice) the parent Hamiltonian can be gapless with a critical ground state. To show this we invoke a mapping between classical and quantum models and prove that in these cases the injectivity relation between boundary and bulk solely depends on the lattice geometry.Comment: 8 page

THE LECTOTYPE AND TYPE LOCALITY OF CANDOIA CARINATA (REPTILIA, SERPENTES)

The lectotype of Boa carinata Schneider, 1801, has been rediscovered and is now ZMFK35503, having been transferred in 1977 from the Göttingen Museum, its original location. Its typelocality has not been reported previously, but is indicated as “Amboina” on a label with the lectotype.The specimen is redescribed and fi gured, and conforms with expectations for material from the vicinityof the type locality. A brief history of Schneider and of the syntypes of Boa carinata, now Candoiacarinata, is reviewed

A comparative study of the public relations programs and policies in schools and businesses

Thesis (Ed.M.)--Boston Universit

A serological investigation of caseous lymphadenitis in four flocks of sheep

A double antibody sandwich ELISA developed by ID-DLO, Lelystad to detect Corynebocterium pseudotuberculosis infection was used on 329 sheep from four pedigree Suffolk flocks in which clinical cases of caseous lymphadenitis (CLA) had occurred. At subsequent necropsy, typical CLA lesions were seen in 133 sheep, and the diagnosis was confirmed on culture. Lesions were most commonly seen in lungs (n = 46), parotid lymph nodes (n = 44), prescapular lymph nodes (n = 38) and mediastinal lymph nodes (n = 31). The sensitivity of the ELISA test for detecting culture-positive sheep was 0.88, while the specificity of the test was 0.55. The antibody ELISA detected 87.5 per cent of sheep that had CLA lesions restricted to internal organs only. It was concluded that the ELISA test has a valuable role in detecting sheep with both clinical and subclinical CLA

The Composite Spectrum of Strong Lyman-alpha Forest Absorbers

We present a new method for probing the physical conditions and metal enrichment of the Intergalactic Medium: the composite spectrum of Ly-alpha forest absorbers. We apply this technique to a sample of 9480 Ly-alpha absorbers with redshift 2 < z < 3.5 identified in the spectra of 13,279 high-redshift quasars from the Sloan Digital Sky Survey (SDSS) Fifth Data Release (DR5). Absorbers are selected as local minima in the spectra with 2.4 < tau_Ly-alpha < 4.0; at SDSS resolution (~ 150km/s FWHM), these absorbers are blends of systems that are individually weaker. In the stacked spectra we detect seven Lyman-series lines and metal lines of O VI, N V, C IV, C III, Si IV, C II, Al II, Si II, Fe II, Mg II, and O I. Many of these lines have peak optical depths of < 0.02, but they are nonetheless detected at high statistical significance. Modeling the Lyman-series measurements implies that our selected systems have total H I column densities N_HI ~ 10^15.4cm-2. Assuming typical physical conditions rho / = 10, T = 10^4 - 10^4.5 K, and [Fe/H]= -2 yields reasonable agreement with the line strengths of high-ionization species, but it underpredicts the low-ionization species by two orders of magnitude or more. This discrepancy suggests that the low ionization lines arise in dense, cool, metal-rich clumps, present in some absorption systems.Comment: 7 pages, 4 figures, 1 table, accepted by ApJL, revisions mad