HUMAN RESOURCE POLICIES FOR NONMETROPOLITAN AMERICA

Labor and Human Capital,

The Use of Multi-beam Sonars to Image Bubbly Ship Wakes

During the past five years, researchers at Penn State University (PSU) have used upward-looking multi-beam (MB) sonar to image the bubbly wakes of surface ships. In 2000, a 19-beam, 5° beam width, 120° sector, 250 kHz MB sonar integrated into an autonomous vehicle was used to obtain a first-of-a-kind look at the three-dimensional variability of bubbles in a large ship wake. In 2001 we acquired a Reson 8101 MB sonar, which operates at 240 kHz and features 101-1.5º beams spanning a 150º sector. In July 2002, the Reson sonar was deployed looking upward from a 1.4 m diameter buoy moored at 29.5 m depth in 550 m of water using three anchor lines. A fiber optic cable connected the sonar to a support ship 500 m away. Images of the wake of a small research vessel provided new information about the persistence of bubble clouds in the ocean. An important goal is to use the MB sonar to estimate wake bubble distributions, as has been done with single beam sonar. Here we show that multipath interference and strong, specular reflections from the sea surface adversely affect the use of MB sonars to unambiguously estimate wake bubble distributio

Innovative activity in rural areas: the importance of local and regional characteristics

Innovation, supported by a developed and active entrepreneurial system, long has been recognized as critical to regional economic competitiveness. Innovation also plays an essential role for rural economic development as these regions respond to the challenges of competing in the global economy. Barkley and Henry identify assets that contribute to nonmetro innovation “hot spots.”Community development ; Research and development ; Patents

Innovative Activity in Rural Areas: The Role of Local and Regional Characteristics

Community/Rural/Urban Development,

Stem cell mechanobiology

Stem cells are undifferentiated cells that are capable of proliferation, self-maintenance and differentiation towards specific cell phenotypes. These processes are controlled by a variety of cues including physicochemical factors associated with the specific mechanical environment in which the cells reside. The control of stem cell biology through mechanical factors remains poorly understood and is the focus of the developing field of mechanobiology. This review provides an insight into the current knowledge of the role of mechanical forces in the induction of differentiation of stem cells. While the details associated with individual studies are complex and typically associated with the stem cell type studied and model system adopted, certain key themes emerge. First, the differentiation process affects the mechanical properties of the cells and of specific subcellular components. Secondly, that stem cells are able to detect and respond to alterations in the stiffness of their surrounding microenvironment via induction of lineage-specific differentiation. Finally, the application of external mechanical forces to stem cells, transduced through a variety of mechanisms, can initiate and drive differentiation processes. The coalescence of these three key concepts permit the introduction of a new theory for the maintenance of stem cells and alternatively their differentiation via the concept of a stem cell 'mechano-niche', defined as a specific combination of cell mechanical properties, extracellular matrix stiffness and external mechanical cues conducive to the maintenance of the stem cell population.<br/

SOUTHERNMOST OCCURRENCE OF THE SUWANNEE COOTER, PSEUDEMYS CONCINNA SUWANNIENSIS (TESTUDINES: EMYDIDAE)

The Suwannee Cooter, Pseudemys concinna suwanniensis, the largest member of the speciose turtle family Emydidae, inhabits a small number of rivers that drain into the northeastern Gulf of Mexico along the northwest coast of Florida from just west of Tallahassee to just south of Tampa. The status of this state-protected subspecies in the southernmost of these rivers, the Alafia, is unknown and hence of conservation concern. We provide recent evidence confirming that a reproducing population still exists in this river, and review available specimens and both published and unpublished records documenting the southern limit of distribution. At least within the eastern United States, our observations also extend confirmed knowledge of the geographic occurrence of hatchling turtles overwintering in the nest southward by 285 km

Radiolabeling human peripheral blood stem cells for positron emission tomography (PET) imaging in young rhesus monkeys.

These studies focused on a new radiolabeling technique with copper ((64)Cu) and zirconium ((89)Zr) for positron emission tomography (PET) imaging using a CD45 antibody. Synthesis of (64)Cu-CD45 and (89)Zr-CD45 immunoconjugates was performed and the evaluation of the potential toxicity of radiolabeling human peripheral blood stem cells (hPBSC) was assessed in vitro (viability, population doubling times, colony forming units). hPBSC viability was maintained as the dose of (64)Cu-TETA-CD45 increased from 0 (92%) to 160 µCi/mL (76%, p&gt;0.05). Radiolabeling efficiency was not significantly increased with concentrations of (64)Cu-TETA-CD45 &gt;20 µCi/mL (p&gt;0.50). Toxicity affecting both growth and colony formation was observed with hPBSC radiolabeled with ≥40 µCi/mL (p&lt;0.05). For (89)Zr, there were no significant differences in viability (p&gt;0.05), and a trend towards increased radiolabeling efficiency was noted as the dose of (89)Zr-Df-CD45 increased, with a greater level of radiolabeling with 160 µCi/mL compared to 0-40 µCi/mL (p&lt;0.05). A greater than 2,000 fold-increase in the level of (89)Zr-Df-CD45 labeling efficiency was observed when compared to (64)Cu-TETA-CD45. Similar to (64)Cu-TETA-CD45, toxicity was noted when hPBSC were radiolabeled with ≥40 µCi/mL (p&lt;0.05) (growth, colony formation). Taken together, 20 µCi/mL resulted in the highest level of radiolabeling efficiency without altering cell function. Young rhesus monkeys that had been transplanted prenatally with 25×10(6) hPBSC expressing firefly luciferase were assessed with bioluminescence imaging (BLI), then 0.3 mCi of (89)Zr-Df-CD45, which showed the best radiolabeling efficiency, was injected intravenously for PET imaging. Results suggest that (89)Zr-Df-CD45 was able to identify engrafted hPBSC in the same locations identified by BLI, although the background was high