Interactive effects on AMP bioactivity in terms of Bd growth rate with standard error (Acid x Population p^range = 0.033–0.084, Acidification x Shade x Population p = 0.773).

<p>A. Sun referent. B. Shade referent. Contrast indicates significant simple effect of Acidification within full sun (NoShade) treatments of the Northern Population (p = 0.018). Full ANCOVA results can be found in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0130383#pone.0130383.s005" target="_blank">S5 Table</a>.</p

Average time to metamorphosis with standard error.

<p>Both Shade and Population were significant predictors of mean larval duration under all treatment combinations (shade p^range = 0.003 to 0.04; population p = 9.5 x10^-5–0.017). Figure displays results of Population effects within Acidified environments. Full ANOVA outputs can be found in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0130383#pone.0130383.s001" target="_blank">S1 Table</a>.</p

Interaction effects on AMP production (µg/ml standardized by gram body weight) with standard error (Acidification x Shade p = 0.0272; Population x Shade p^range = 0.0501–0.7868).

<p>A. Northern referent. B. Southern referent. C. Acidified referent. Referent variables refer to a specific treatment environment, indicating what two-way interaction is being displayed. Contrasts indicate significant simple effects within each two-way interaction (p<0.05) (eg. A. indicates a significant Acidification effect within the NoShade treatments and a significant Shade effect within the Acidified treatments in the northern Population) [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0130383#pone.0130383.ref062" target="_blank">62</a>,<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0130383#pone.0130383.ref063" target="_blank">63</a>]. Full ANCOVA outputs can be found in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0130383#pone.0130383.s003" target="_blank">S3 Table</a>.</p

Clone library comparison between larval and post-metamorphic (juvenile) <i>R</i>. <i>catesbeiana</i> skin-associated bacteria.

<p>The percent of the clone library represented by each taxonomic group is shown. (Larvae library: N = 78, Juvenile library: N = 83)</p

NMDS ordination plot of <i>R</i>. <i>catesbeiana</i> larval and juvenile frog microbial community similarity by acidification treatment.

<p>N = 152 after outlier analysis (McCune and Grace 2002). Ordination stress = 20. Axes display percentage variance explained. Circles designate juvenile frog microbial communities, triangles designate larval microbial communities. Open symbols designate acidified pH treatments while closed symbols designate un-manipulated pH treatments.</p

Population effect on mean juvenile mass (g) at sample collection with standard error.

<p>Population and Days in lab were significant predictors of juvenile mass in many but not all treatment environments (Population p^range = 0.007 to 0.0866; Days in lab p = 0.0042). Figure displays results of Population effects within Shaded and Acidified treatments. Full ANCOVA outputs can be found in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0130383#pone.0130383.s002" target="_blank">S2 Table</a>.</p

Fun_AVE_Live.data_matrix

Fungal community data matrix (see meta-data)

NewickTree

Plant phylogeny (see meta-data)

gps_collection_locations

The spatial locations of soil samples

Bact_AVE_Live.data_matrix

Bacterial community data matrix (see meta-data)