Bioavailability and mixture effects of metals in different European mussel populations

More than 100 million tons of chemicals that have the potential to pose a risk to the environment are produced in Europe each year. A subset of these chemicals may, intentionally or not, enter and affect the environment. To protect the environment and the diverse services it provides, it is important to know what the impact and risk of a chemical release may be. Underestimating the risk can have harmful effects on the environment and on human health. Overestimating the risks may, unnecessarily, increase the costs of preventing or ameliorating pollution. Hence, accurate knowledge of the effects and the associated risks is essential. Predicting the effect of a chemical is, currently, primarily based on the results of singlespecies experiments with freshwater organisms that are exposed to a single stressor in a standardized (laboratory) environment. However, in reality organisms are not exposed to these standardized conditions, but live in and are exposed to a variable environment. Furthermore, inter-population differences in sensitivity may exist due to differences in local adaptation and even a single organism’s sensitivity may change during its lifetime. Finally, organisms may be exposed to multiple stressors, natural or anthropogenic, simultaneously. Hence, it is suggested that it might not be possible to accurately predict the adverse effects using the currently prescribed methods. The main objective of this research was to examine the effect of these potential sources of variation on the toxicity of chemicals on marine organisms in order to increase the realism of current environmental risk assessment procedures. This was accomplished by assessing the influence of environmental variation, mixture toxicity, population variability and life-stage variation on the accumulation and toxicity of Cu on a Cu sensitive marine test species, the mussel

Non-contact optical tweezers-based single cell analysis through in vivo X-ray elemental imaging

We report on a radically new elemental imaging approach for the analysis of biological model organisms and single cells in their natural, in vivo state. The methodology combines optical tweezers (OT) technology for non-contact, laser-based sample manipulation with synchrotron radiation confocal X-ray fluorescence (XRF) microimaging for the first time. The main objective of this work is to establish a new method for in situ elemental imaging of free-standing living biological microorganisms or single cells in their aqueous environment. Using the model organism Scrippsiella trochoidea, several successful test experiments focussing on applications in environmental toxicology have been performed at ESRF-ID13, demonstrating the feasibility, repeatability and high throughput potential of the OT XRF methodology. We expect that the OT XRF methodology will significantly contribute to the new trend of investigating microorganisms at the cellular level with added in vivo capability

In vivo X-ray elemental imaging of single cell model organisms manipulated by laser-based optical tweezers

We report on a radically new elemental imaging approach for the analysis of biological model organisms and single cells in their natural, in vivo state. The methodology combines optical tweezers (OT) technology for non-contact, laser based sample manipulation with synchrotron radiation confocal X-ray fluorescence (XRF) microimaging for the first time. The main objective of this work is to establish a new method for in vivo elemental imaging in a two-dimensional (2D) projection mode in free-standing biological microorganisms or single cells, present in their aqueous environment. Using the model organism Scrippsiella trochoidea, a first proof of principle experiment at beamline ID13 of the European Synchrotron Radiation Facility (ESRF) demonstrates the feasibility of the OT XRF methodology, which is applied to study mixture toxicity of Cu-Ni and Cu-Zn as a result of elevated exposure. We expect that the new OT XRF methodology will significantly contribute to the new trend of investigating microorganisms at the cellular level with added in vivo capability

The Effects of Density on the Growth and Temperature Production of <i>Tenebrio molitor</i> Larvae

Tenebrio molitor larvae live, at least partially, inside their feed. Hence, they do not live on a 2D plane but in a 3D environment. However, previous studies mainly focused on the optimal number of larvae for a given surface area, not the available volume. The goal of this study was to assess the growth and survival of mealworms in a standardized semi-industrial setting with a varying density (cm3) and substrate height. A full factorial experimental design was used with five larval densities (0.5–8 larvae/cm3) and four feed heights (1–8 cm) in 60 × 40 cm crates. Furthermore, the in-crate temperature was monitored and linked to the density. The results of this study clearly indicate that mealworm larvae prefer a low density (cm3). At low larvae densities, the substrate height was less important, with a slight preference for a thicker layer. In contrast, at high(er) larval densities, a lower layer thickness resulted in better growth. The in-crate week temperature varied up to 14 °C (25–39 °C) between treatments and could be predicted well based on the number and size of the larvae. These results may help the industry to improve their production efficiency in terms of larvae density, substrate height and room temperature

The Effects of Density on the Growth and Temperature Production of Tenebrio molitor Larvae

Tenebrio molitor larvae live, at least partially, inside their feed. Hence, they do not live on a 2D plane but in a 3D environment. However, previous studies mainly focused on the optimal number of larvae for a given surface area, not the available volume. The goal of this study was to assess the growth and survival of mealworms in a standardized semi-industrial setting with a varying density (cm3) and substrate height. A full factorial experimental design was used with five larval densities (0.5&ndash;8 larvae/cm3) and four feed heights (1&ndash;8 cm) in 60 &times; 40 cm crates. Furthermore, the in-crate temperature was monitored and linked to the density. The results of this study clearly indicate that mealworm larvae prefer a low density (cm3). At low larvae densities, the substrate height was less important, with a slight preference for a thicker layer. In contrast, at high(er) larval densities, a lower layer thickness resulted in better growth. The in-crate week temperature varied up to 14 &deg;C (25&ndash;39 &deg;C) between treatments and could be predicted well based on the number and size of the larvae. These results may help the industry to improve their production efficiency in terms of larvae density, substrate height and room temperature

Habitat use, but not dispersal limitation, as the mechanism behind the aggregated population structure of the mygalomorph species Atypus affinis

International audienceDispersal and habitat selection are the main factors that affect the distribution of species in spatially structured habitat. Species typically occurring in an aggregated way are supposed to experience dispersal limitation or to be highly selective for specific habitat attributes in their environment. In order to understand the distribution pattern of a mygalomorph spider species, Atypus affinis, we conducted an intensive survey to detect correlations of spider densities with specific habitat variables and empirically tested the dispersal propensity of spiderlings. In the field, the spiders exhibited an aggregated distribution correlated with patches of heathlands (dominated by Calluna vulgaris). Contrary to our expectations, laboratory experiments revealed a very high dispersal propensity in juveniles (more than 80% of individuals dispersed at least once during two experiments). This dispersal was strongly context dependent with a pronounced negative effect of starvation and a positive effect of clutch size. Kin competition is hypothezised to be the driving force behind these high dispersal abilities. The aggregation of A. affinis is a likely result of habitat use rather than dispersal limitation

Mixture toxicity in the marine environment : model development and evidence for synergism at environmental concentrations

Little is known about the effect of metal mixtures on marine organisms, especially after exposure to environmentally realistic concentrations. This information is, however, required to evaluate the need to include mixtures in future environmental risk assessment procedures. We assessed the effect of copper (Cu)-Nickel (Ni) binary mixtures on Mytilus edulis larval development using a full factorial design that included environmentally relevant metal concentrations and ratios. The reproducibility of the results was assessed by repeating this experiment 5 times. The observed mixture effects were compared with the effects predicted with the concentration addition model. Deviations from the concentration addition model were estimated using a Markov chain Monte-Carlo algorithm. This enabled the accurate estimation of the deviations and their uncertainty. The results demonstrated reproducibly that the type of interactionsynergism or antagonismmainly depended on the Ni concentration. Antagonism was observed at high Ni concentrations, whereas synergism occurred at Ni concentrations as low as 4.9g Ni/L. This low (and realistic) Ni concentration was 1% of the median effective concentration (EC50) of Ni or 57% of the Ni predicted-no-effect concentration (PNEC) in the European Union environmental risk assessment. It is concluded that results from mixture studies should not be extrapolated to concentrations or ratios other than those investigated and that significant mixture interactions can occur at environmentally realistic concentrations. This should be accounted for in (marine) environmental risk assessment of metals