SerpinB3 promotes pro-fibrogenic responses in activated hepatic stellate cells

SerpinB3 is a hypoxia- and hypoxia-inducible factor-2\u3b1-dependent cystein protease inhibitor that is up-regulated in hepatocellular carcinoma and in parenchymal cells during chronic liver diseases (CLD). SerpinB3 up-regulation in CLD patients has been reported to correlate with the extent of liver fibrosis and the production of transforming growth factor-\u3b21, but the actual role of SerpinB3 in hepatic fibrogenesis is still poorly characterized. In the present study we analyzed the pro-fibrogenic action of SerpinB3 in cell cultures and in two different murine models of liver fibrosis. "In vitro" experiments revealed that SerpinB3 addition to either primary cultures of human activated myofibroblast-like hepatic stellate cells (HSC/MFs) or human stellate cell line (LX2 cells) strongly up-regulated the expression of genes involved in fibrogenesis and promoted oriented migration, but not cell proliferation. Chronic liver injury by CCl4 administration or by feeding a methionine/choline deficient diet to transgenic mice over-expressing human SerpinB3 in hepatocytes confirmed that SerpinB3 over-expression significantly increased the mRNA levels of pro-fibrogenic genes, collagen deposition and \u3b1SMA-positive HSC/MFs as compared to wild-type mice, without affecting parenchymal damage. The present study provides for the first time evidence that hepatocyte release of SerpinB3 during CLD can contribute to liver fibrogenesis by acting on HSC/MFs

Prevalence of new and known species of haemoparasites in feral pigeons in northwest Italy

Background: Haemoparasites in feral pigeons have been studied in several countries but no data are available from Italy. The aim of this work was to evaluate the prevalence and diversity of Haemoproteus spp./Plasmodium spp. and Leucocytozoon spp. in feral pigeons from northwest Italy, as well as the association between infection and host age or sex. Methods: Feral pigeons were collected during a regional culling programme from the Piedmont region (northwest Italy) and subjected to necropsy. Infections were detected from DNA extracted from the spleen following a nested PCR protocol. The association between sex or age and infection status was evaluated using the chi-squared test for independence or Fisher’s exact test. Results: Out of 51 animals, 15 were positive for Haemoproteus/Plasmodium spp. and eight for Leucocytozoon spp., with a significant difference between haemoparasites prevalence. There was no significant association between age or sex and infection status. The coinfection with different haemoparasites was very significant (p < 0.01), showing a greater relative risk to be infected by a second haemoparasite in birds already infected, in particular in male and in adult pigeons. DNA sequencing of Leucocytozoon spp. showed six different lineages in pigeons, and one of Haemoproteus and Plasmodium, respectively. Conclusions: Blood parasites are continuously circulating around the world, and the results presented in the paper suggest that cross infection of feral pigeons with haemoparasites typical of other migratory or nonmigratory bird species is possible. Moreover, the geographical location of Italy along the main migratory routes is a crucial factor to be considered for migratory birds, because they can be affected by blood parasites detected in feral pigeons, and vice versa

ACEWATER2 Regional database: hydro-climatology data-analysis

The report presents the architecture of a regional hydro-climatology information system, developed in the framework of the ACEWATER2 project, in order to support effective organization of information. Information includes both freely available large and regional scale data sources, as well as databases compiled by the CoEs (Centers of Excellence) and submitted as part of their scientific undertakings. The information system builds upon and specializes the JRC knowledge sharing platform Aquaknow (https://aquaknow.jrc.ec.europa.eu/), including: • at the system core, a relational database; its schema has been designed to store both detailed metadata and, where relevant (avoiding duplication of information otherwise accessible), data themselves. Metadata include, among others, datasets extended description, spatial extent, temporal frequency, reference Institutions/authors, credits and limitations, web links to access original data and/or any further documentation. Data can be stored as public or private, depending upon confidentialy and sharing policies; • user friendly facilities, supporting the end user in efficiently browsing, querying, uploading and downloading information (metadata and data). System access is limited to accredited audience, via password authentication. Dedicated groups for the three ACEWATER CoE networks (Western, Southern and Central-Eastern Africa) have been setup and scientists invited to register. Currently the system is operational and we submitted databases documented and, depending upon confidentiality and authorization issues, also stored. A general review and classification of freely available information at continenal, regional and local scale of interest to ACEWATER2 project, and particularly to selected study areas (Senegal, Gambia and Niger; Zambezi; Blue Nile and Lake Victoria), have been completed. Metadata and, where relevant, data themselves have been stored to the information system database. Information submitted by the CoE (a continuous ongoing process) is migrated to the database as well, depending upon sharing authorization and/or limitations. The report also documents the ongoing scientific research at JRC on climate variability analysis based on L-Moments statistics. In particular maps of estimated precipitation deficit for different return periods at the river basins of interest are presented and included in the database.JRC.D.2-Water and Marine Resource

On Expressing and Monitoring Oscillatory Dynamics

To express temporal properties of dense-time real-valued signals, the Signal Temporal Logic (STL) has been defined by Maler et al. The work presented a monitoring algorithm deciding the satisfiability of STL formulae on finite discrete samples of continuous signals. The logic has been used to express and analyse biological systems, but it is not expressive enough to sufficiently distinguish oscillatory properties important in biology. In this paper we define the extended logic STL* in which STL is augmented with a signal-value freezing operator allowing us to express (and distinguish) detailed properties of biological oscillations. The logic is supported by a monitoring algorithm prototyped in Matlab. The monitoring procedure of STL* is evaluated on a biologically-relevant case study.Comment: In Proceedings HSB 2012, arXiv:1208.315

Absence of Viable Toxoplasma gondii in Artisanal Raw-Milk Ewe Cheese Derived from Naturally Infected Animals

The presence of viable Toxoplasma gondii was investigated in artisanal cheeses made from milk of naturally infected ewes. Ewe milk was analyzed beforehand for the presence and vitality of T. gondii by loop-mediated isothermal amplification (LAMP) and reverse-transcriptase PCR (RT-PCR), respectively. Cheeses were prepared from raw milk following a traditional cheesemaking process. The cheese obtained from T. gondii-positive milk was analyzed by LAMP to detect Toxoplasma DNA-positive samples. RT-PCR was then carried out to assess the viability of the parasites in T. gondii-positive milk samples and fresh cheese, after 5 and 15 days of ripening. Physical-chemical parameters of cheeses were also investigated. All cheese samples derived from T. gondii-positive milk were positive according to LAMP, at both 5 and 15 days of ripening, while none of the samples were positive according to RT-PCR. Thus, while the presence of the parasite was demonstrated by the detection of specific DNA, the absence of detectable T. gondii RNA supports the hypothesis that changes in the chemical and physical characteristics occurring during the cheesemaking process and ripening period, could be sufficient to inactivate viable T. gondii in milk, minimizing the risk of human infection through consumption of raw sheep milk cheese