Probing Fistulae in the G.I. Tract

Fistulae are commonly encountered in general surgery. A fistula is an abnormal connection between two epithelial surfaces (figure 1). It can connect two hollow viscera together, or can connect a hollow viscus to the skin. In this way they can either be visceral and internal, or cutaneous and external. The connection between the surfaces is usually lined with granulation tissue. A sinus is a granulating track from a source of infection to the surface. Sinuses can develop into fistulae if inadequately treated.

Drug Trafficking Routes and Hepatitis B in Injection Drug Users, Manipur, India

Prevalence of hepatitis B genotype C in injection drug users in the northeastern Indian state of Manipur, neighboring the "Golden Triangle," correlates well with overland drug-trafficking routes, the injection drug use epidemic, and the spread of HIV. Further spread to other regions of India through mobile populations is possible

Systematics of Anti magnetic rotation in even-even Cd isotopes

The lifetimes for the high spin levels of the yrast band of $^{110}$Cd has been measured. The estimated B(E2) values decrease with increase in angular momentum. This is the characteristic of Anti magnetic rotation as reported in $^{106,108}$Cd. However, alignment behavior of $^{110}$Cd is completely different from its even-even neighbors. A model based on classical particle plus rotor has been used to explore the underlying systematics and develop a self consistent picture for the observed behavior of these isotopes.Comment: 18 pages including 6 figures. Accepted to be published in PLB, with some modification in the tex

Large Mass Diphotons From Relativistic Heavy Ion Collisions

We evaluate the production of large mass diphotons from quark annihilation at BNL RHIC and CERN LHC energies from central collisions of gold nuclei. The collision is assumed to lead to either a thermally and chemically equilibrated quark gluon plasma, or a free-streaming quark gluon gas having an identical initial entropy, or a chemically equilibrating quark gluon system, with the same entropy at $T=T_c$. We also obtain an estimate of hard photon pairs from initial state quark annihilation and find that the thermal production dominates the yield up to $M \approx$ 4 GeV at RHIC, and up to 6 GeV at LHC. A simulation study of decay versus thermal diphotons is presented.Comment: Latex file; to appear in Physics Letters

Anti-hepatitis B core antigen testing with detection and characterization of occult hepatitis B virus by an in-house nucleic acid testing among blood donors in Behrampur, Ganjam, Orissa in southeastern India: implications for transfusion

<p>Abstract</p> <p>Background</p> <p>Occult hepatitis B virus (HBV) infection might transmit viremic units into the public blood supply if only hepatitis B surface antigen (HBsAg) testing is used for donor screening. Our aim was to evaluate the prevalence of occult HBV infection among the HBsAg negative/antiHBc positive donations from a highly HIV prevalent region of India.</p> <p>Methods</p> <p>A total of 729 HBsAg negative donor units were included in this study. Surface gene and precore region were amplified by in house nucleic acid test (NAT) for detection of occult HBV infection and surface gene was analyzed after direct sequencing.</p> <p>Results</p> <p>A total of 220 (30.1%) HBsAg negative donors were antiHBc positive, of them 66 (30%) were HBV DNA positive by NAT. HBV DNA positivity among 164 antiHBc only group, was 27.1% and among 40 antiHBs positive group was 30.0%. HBV/D (93.3%) was predominant and prevalence of both HBV/C and HBV/A was 3.3%. Single or multiple amino acids substitutions were found in 95% samples.</p> <p>Conclusion</p> <p>Thus, a considerable number of HBV infected donors remain undiagnosed, if only HBsAg is used for screening. Addition of antiHBc testing for donor screening, although will lead to rejection of a large number of donor units, will definitely eliminate HBV infected donations and help in reducing HBV transmission with its potential consequences, especially among the immunocompromised population. The HBV genetic diversity found in this donor population are in accordance with other parts of India.</p

Quark Gluon Plasma Diagnostics in a Successive Equilibrium Scenario

The relativistic Fokker Planck equation has been used to study the evolution of the quark distribution in the quark gluon phase expected to be formed in ultra-relativistic heavy ion collisions. The effect of thermal masses for quarks and gluons is incorporated to take account of the in-medium properties. We find that the kinetic equilibrium is achieved before the system reaches the critical temperature of quark hadron phase transition. We find that chemical equilibrium is not achieved during this time. We have evaluated the electromagnetic probes of quark gluon plasma from the non-equilibrated quark gluon phase and compared them with those in completely equilibrated scenario. The hard QCD production rates for the electromagnetic ejectiles as well as the heavy quark production rates are also calculated.Comment: Submitted to Nucl. Phys.

Quark Hadron Phase Transition and Hybrid Stars

We investigate the properties of hybrid stars consisting of quark matter in the core and hadron matter in outer region. The hadronic and quark matter equations of state are calculated by using nonlinear Walecka model and chiral colour dielectric (CCD) model respectively. We find that the phase transition from hadron to quark matter is possible in a narrow range of the parameters of nonlinear Walecka and CCD models. The transition is strong or weak first order depending on the parameters used. The EOS thus obtained, is used to study the properties of hybrid stars. We find that the calculated hybrid star properties are similar to those of pure neutron stars.Comment: 25 pages in LaTex and 9 figures available on request, IP/BBSR/94-3

A Rare HBV Subgenotype D4 with Unique Genomic Signatures Identified in North-Eastern India –An Emerging Clinical Challenge?

BACKGROUND/AIMS: HBV has been classified into ten genotypes (A-J) and multiple subgenotypes, some of which strongly influence disease outcome and their distribution also correlate with human migration. HBV infection is highly prevalent in India and its diverse population provides an excellent opportunity to study the distinctiveness of HBV, its evolution and disease biology in variegated ethnic groups. The North-East India, having international frontiers on three sides, is one of the most ethnically and linguistically diverse region of the country. Given the paucity of information on molecular epidemiology of HBV in this region, the study aimed to carry out an in-depth genetic characterization of HBV prevailing in North-East state of Tripura. METHODS: From sera of chronically HBV infected patients biochemical/serological tests, HBV DNA quantification, PCR-amplification, sequencing of PreS/S or full-length HBV genomes were done. HBV genotype/subgenotype determination and sequence variability were assessed by MEGA5-software. The evolutionary divergence times of different HBV subgenotypes were estimated by DNAMLK/PHYLIP program while jpHMM method was used to detect any recombination event in HBV genomes. RESULTS: HBV genotypes D (89.5%), C (6.6%) and A (3.9%) were detected among chronic carriers. While all HBV/A and HBV/C isolates belonged to subgenotype-A1 and C1 respectively, five subgenotypes of HBV/D (D1-D5) were identified including the first detection of rare D4. These non-recombinant Indian D4 (IndD4) formed a distinct phylogenetic clade, had 2.7% nucleotide divergence and recent evolutionary radiation than other global D4. Ten unique amino acids and 9 novel nucleotide substitutions were identified as IndD4 signatures. All IndD4 carried T120 and R129 in ORF-S that may cause immune/vaccine/diagnostic escape and N128 in ORF-P, implicated as compensatory Lamivudine resistance mutation. CONCLUSIONS: IndD4 has potential to undermine vaccination programs or anti-viral therapy and its introduction to North-East India is believed to be linked with the settlement of ancient Tibeto-Burman migrants from East-Asia

First Case of COVID-19 in the Far Western Province of Nepal

Recently emerged worldwide public health problem coronavirus disease (COVID-19) is an infectious disease caused by a severe acute respiratory syndrome coronavirus 2(SARS Cov-2); end of 2019  it was identified as the cause of a cluster of pneumonia cases in Wuhan, a city in the Hubei Province of China. It rapidly spread, resulting in an epidemic throughout China, followed by pandemic throughout the world as well as in Nepal. Finally far western province became the fourth case of Nepal and index case of this province. A 34 year old male presented Seti Provincial hospital fever clinic with the complaints of fever, cough, sore throat and history of travel from Dubai to Nepal. He was kept in isolation and throat swab result was positive for SARS CoV-2. Other systemic examination and routine investigation were within normal. Course of illness was uneventful and managed conservatively. First, second and third RT-PCR became positive and fourth and fifth turned into negative and discharged at the 29th day of admission

Re-establishing Responsiveness in a Case of Refractory Metastatic Rectal Cancer with a Personalized de novo Combination Regimen

Introduction: Encyclopedic Tumor Analysis (ETA) is multi-analyte, molecular and functional interrogation to identify latent vulnerabilities in solid tumors which can then be targeted in organ- and label-agnostic combination treatment regimens.Case Presentation: We describe here a case of metastatic rectal cancer in a 61-year-old male who was progressed on all prior Standard of Care (SoC) treatment modalities including surgery, chemotherapy and radiotherapy. We addressed disease recurrence via personalized therapy guided by ETA which revealed characteristic molecular heterogeneity in primary and metastatic lesions in terms of single nucleotide variations (SNVs) and gene copy number variations (CNVs).  Notably, a novel TBL1XR1 (Exon1) – PIK3CA (Exon 2) gene fusion was identified in the tumor along with gene copy number gains in TERT, IGF-1R, MYC, FGFR1 and EGFR genes.Conclusion: ETA based molecular analysis with synchronous in vitro chemo-sensitivity profiling strategy helped to define de novo combinatorial therapy regimen of targeted and cytotoxic drugs which countered disease progression at each instance and led to the durable regression of primary as well as metastatic lesions