386 research outputs found
The Role of Maternal Thyroid Hormones in Avian Embryonic Development
During avian embryonic development, thyroid hormones (THs) coordinate the expression of a multitude of genes thereby ensuring that the correct sequence of cell proliferation, differentiation and maturation is followed in each tissue and organ. Although THs are needed from the start of development, the embryonic thyroid gland only matures around mid-incubation in precocial birds and around hatching in altricial species. Therefore, maternal THs deposited in the egg yolk play an essential role in embryonic development. They are taken up by the embryo throughout its development, from the first day till hatching, and expression of TH regulators such as distributor proteins, transporters, and deiodinases in the yolk sac membrane provide the tools for selective metabolism and transport starting from this level. TH receptors and regulators of local TH availability are expressed in avian embryos in a dynamic and tissue/cell-specific pattern from the first stages studied, as shown in detail in chicken. Maternal hyperthyroidism via TH supplementation as well as injection of THs into the egg yolk increase TH content in embryonic tissues while induction of maternal hypothyroidism by goitrogen treatment results in a decrease. Both increase and decrease of maternal TH availability were shown to alter gene expression in early chicken embryos. Knockdown of the specific TH transporter monocarboxylate transporter 8 at early stages in chicken cerebellum, optic tectum, or retina allowed to reduce local TH availability, interfering with gene expression and confirming that development of the central nervous system (CNS) is highly dependent on maternal THs. While some of the effects on cell proliferation, migration and differentiation seem to be transient, others result in persistent defects in CNS structure. In addition, a number of studies in both precocial and altricial birds showed that injection of THs into the yolk at the start of incubation influences a number of parameters in posthatch performance and fitness. In conclusion, the data presently available clearly indicate that maternal THs play an important role in avian embryonic development, but how exactly their influence on cellular and molecular processes in the embryo is linked to posthatch fitness needs to be further explored
Thyroid Hormone Receptors in Two Model Species for Vertebrate Embryonic Development: Chicken and Zebrafish
Chicken and zebrafish are two model species regularly used to study the role of thyroid hormones in vertebrate development. Similar to mammals, chickens have one thyroid hormone receptor α (TRα) and one TRβ gene, giving rise to three TR isoforms: TRα, TRβ2, and TRβ0, the latter with a very short amino-terminal domain. Zebrafish also have one TRβ gene, providing two TRβ1 variants. The zebrafish TRα gene has been duplicated, and at least three TRα isoforms are expressed: TRαA1-2 and TRαB are very similar, while TRαA1 has a longer carboxy-terminal ligand-binding domain. All these TR isoforms appear to be functional, ligand-binding receptors. As in other vertebrates, the different chicken and zebrafish TR isoforms have a divergent spatiotemporal expression pattern, suggesting that they also have distinct functions. Several isoforms are expressed from the very first stages of embryonic development and early chicken and zebrafish embryos respond to thyroid hormone treatment with changes in gene expression. Future studies in knockdown and mutant animals should allow us to link the different TR isoforms to specific processes in embryonic development
Product lifecycle and choice of transportation modes: Japan’s evidence of import and export
Here we test the hypothesis that commodities at their peak valuation are transported by air and those at their birth and maturity are shipped by sea, and that shippers would choose air for transporting high-valued commodities. We empirically investigated how the product lifecycle of commodities is reflected by shippers' choices of air transportation rather than seaborne transportation. We also assumed that the commodities that achieved substantial innovation in their lifecycles would be moved by air transportation so that these commodities could reach the targeted markets as quickly as possible to avoid the opportunity costs that might be generated by missed business chances. We constructed two unbalanced panel data of 18 commodities (the case of import) and 14 commodities (the case of export) for 24 years from Japan’s custom, demographic, and international statistics. By estimating structural equation systems that consisted of commodity-specific import/export and import/export air ratio functions, we found that the product lifecycle of cargo outgoing from Japan exactly matched the upward and downward move of the air ratio, whereas since incoming commodities are raw materials that have little to do with product lifecycle or matured phase in their lifecycle stage, the peak of commodities’ valuations and the use of air transportation were not necessarily synchronized
Expression of chicken hepatic type I and type III iodothyronine deiodinases during embryonic development
In embryonic chicken liver (ECL) two types of iodothyronine deiodinases
are expressed: D1 and D3. D1 catalyzes the activation as well as the
inactivation of thyroid hormone by outer and inner ring deiodination,
respectively. D3 only catalyzes inner ring deiodination. D1 and D3 have
been cloned from mammals and amphibians and shown to contain a
selenocysteine (Sec) residue. We characterized chicken D1 and D3
complementary DNAs (cDNAs) and studied the expression of hepatic D1 and D3
messenger RNAs (mRNAs) during embryonic development. Oligonucleotides
based on two amino acid sequences strongly conserved in the different
deiodinases (NFGSCTSecP and YIEEAH) were used for reverse
transcription-PCR of poly(A+) RNA isolated from embryonic day 17 (E17)
chicken liver, resulting in the amplification of two 117-bp DNA fragments.
Screening of an E17 chicken liver cDNA library with these probes led to
the isolation of two cDNA clones, ECL1711 and ECL1715. The ECL1711 clone
was 1360 bp long and lacked a translation start site. Sequence alignment
showed that it shared highest sequence identity with D1s from other
vertebrates and that the coding sequence probably lacked the first five
nucleotides. An ATG start codon was engineered by site-directed
mutagenesis, generating a mutant (ECL1711M) with four additional codons
(coding for MGTR). The open reading frame of ECL1711M coded for a
249-amino acid protein showing 58-62% identity with mammalian D1s. An
in-frame TGA codon was located at position 127, which is translated as Sec
in the presence ofa Sec insertion sequence (SECIS) identified in the
3'-untranslated region. Enzyme activity expressed in COS-1 cells by
transfection with ECL1711M showed the same catalytic, substrate, and
inhibitor specificities as native chicken D1. The ECL1715 clone was 1366
bp long and also lacked a translation start site. Sequence alignment
showed that it was most homologous with D3 from other species and that the
coding sequence lacked approximately the first 46 nucleotides. The deduced
amino acid sequence showed 62-72% identity with the D3 sequences from
other species, including a putative Sec residue at a corresponding
position. The 3'-untranslated region of ECL1715 also contained a SECIS
element. These results indicate that ECL1711 and ECL1715 are
near-full-length cDNA clones for chicken D1 and D3 selenoproteins,
respectively. The ontogeny of D1 and D3 expression in chicken liver was
studied between E14 and 1 day after hatching (C1). D1 activity showed a
gradual increase from E14 until C1, whereas D1 mRNA level remained
relatively constant. D3 activity and mRNA level were highly significantly
correlated, showing an increase from E14 to E17 and a strong decrease
thereafter. These results suggest that the regulation of chicken hepatic
D3 expression during embryonic development occurs predominantly at the
pretranslational level
Characterization of a propylthiouracil-insensitive type I iodothyronine deiodinase
Mammalian type I iodothyronine deiodinase (D1) activates and inactivates
thyroid hormone by outer ring deiodination (ORD) and inner ring
deiodination (IRD), respectively, and is potently inhibited by
propylthiouracil (PTU). Here we describe the cloning and characterization
of a complementary DNA encoding a PTU-insensitive D1 from teleost fish
(Oreochromis niloticus, tilapia). This complementary DNA codes for a
protein of 248 amino acids, including a putative selenocysteine (Sec)
residue, encoded by a TGA triplet, at position 126. The 3' untranslated
region contains two putative Sec insertion sequence (SECIS) elements.
Recombinant enzyme expressed in COS-1 cells catalyzes both ORD of T4 and
rT3 and IRD of T3 and T3 sulfate with the same substrate specificity as
native tilapia D1 (tD1), i.e. rT3 >> T4 > T3 sulfate > T3. Native and
recombinant tD1 show equally low sensitivities to inhibition by PTU,
iodoacetate, and gold thioglucose compared with the potent inhibitions
observed with mammalian D1s. Because the residue 2 positions downstream
from Sec is Pro in tD1 and in all (PTU-insensitive) type II and type III
iodothyronine deiodinases but Ser in all PTU-sensitive D1s, we prepared
the Pro128Ser mutant of tD1. The mutant enzyme showed strongly decreased
ORD and somewhat increased IRD activity, but was still insensitive to PTU.
These results provide new information about the structure-activity
relationship of D1 concerning two characteristic properties, i.e.
catalysis of both ORD and IRD, and inhibition by PTU
Effects of experimentally sustained elevated testosterone on incubation behaviour and reproductive success in female great tits (Pants major)
n many seasonally breeding birds, female and male testosterone (T) levels peak at the start of the breeding season, coinciding with pair bonding and nesting activities. Shortly after the onset of egg laying, T levels slowly decline to baseline levels in both sexes, but more rapidly so in females. During this period, T in males may still function to facilitate territorial behaviour, mate guarding and extra pair copulations, either via short lasting peaks or elevated basal levels of the hormone. In some species, however, males become insensitive to increased T after the onset of egg laying. It has been postulated that in these species bi-parental care is essential for offspring survival, as T is known to inhibit paternal care. However, only very few studies have analysed this for females. As females are heavily involved in parental care, they too might become insensitive to T after egg laying. Alternatively, because territorial defence, mate guarding and extra pair copulations are expected to be less important for females than for males, they may not have had the need to evolve a mechanism to become insensitive to T during the period of maternal care, because their natural T levels are never elevated during this part of the breeding season anyway. We tested these alternative hypotheses in female great tits (Parus major). Male great tits have previously been shown to be insensitive to T after egg laying with regard to nestling feeding behaviour (but not song rate). When females had started nest building, we experimentally elevated their T levels up to the nestling feeding phase, and measured incubation behaviour (only females incubate) and reproductive success. T did not significantly affect nest building or egg laying behaviour, although egg laying tended to be delayed in T females. Females with experimentally enhanced T maintained lower temperature during incubation but did not spend less time incubating. This might explain the reduced hatching success of their eggs, smaller brood size and lower number of fledglings we found in this study. As in this species T-dependent behaviour by females during the phase of parental care is not needed, the results support the hypothesis that in this species the need for selection in favour of T-insensitivity did not occur
Changes in the central component of the hypothalamus-pituitary-thyroid axis in a rabbit model of prolonged critical illness
Introduction: Prolonged critically ill patients reveal low circulating thyroid hormone levels without a rise in thyroid stimulating hormone (TSH). This condition is labeled "low 3,5,3'-tri-iodothyronine (T3) syndrome" or "nonthyroidal illness syndrome (NTI)" or "euthyroid sick syndrome". Despite the low circulating and peripheral tissue thyroid hormone levels, thyrotropin releasing hormone (TRH) expression in the hypothalamus is reduced and it remains unclear which mechanism is responsible. We set out to study whether increased hypothalamic T3availability could reflect local thyrotoxicosis and explain feedback inhibition-induced suppression of the TRH gene in the context of the low T3syndrome in prolonged critical illness.Methods: Healthy rabbits were compared with prolonged critically ill, parenterally fed animals. We visualized TRH mRNA in the hypothalamus by in situ-hybridization and measured mRNA levels for the type II iodothyronine diodinase (D2), the thyroid hormone transporters monocarboxylate transporter (MCT) 8, MCT10 and organic anion co-transporting polypeptide 1C1 (OATP1C1) and the thyroid hormone receptors α (TRα) and β (TRβ) in the hypothalamus. We also measured the activity of the D2 and type III iodothyronine deiodinase (D3) enzymes.Results: In the hypothalamus of prolonged critically ill rabbits with low circulating T3 and TSH, we observed decreased TRH mRNA, increased D2 mRNA and increased MCT10 and OATP1C1 mRNA while MCT8 gene expression was unaltered as compared with healthy controls. This coincided with low hypothalamic thyroxine (T4) and low-normal T3concentrations, without a change at the thyroid hormone receptor level.Conclusions: Although expression of D2 and of the thyroid hormone transporters MCT10 and OATP1C1 were increased in the hypothalamus of prolonged critical ill animals, hypothalamic T4and T3content or thyroid hormone receptor expression were not elevated. Hence, decreased TRH gene expression, and hereby low TSH and T3 during prolonged critical illness, is not exclusively brought about by hypothalamic thyrotoxicosis, and infer other TRH suppressing factors to play a role
A role for the melatonin-related receptor GPR50 in leptin signaling, adaptive thermogenesis, and torpor
Effects of dietary macronutrient content on energy metabolism and uncoupling protein mRNA expression in broiler chickens
Thyroid Hormone Transporters MCT8 and OATP1C1 Control Skeletal Muscle Regeneration
Thyroid hormone (TH) transporters are required for the transmembrane passage of TH in target cells. In humans, inactivating mutations in the TH transporter MCT8 cause the Allan-Herndon-Dudley syndrome, characterized by severe neuromuscular symptoms and an abnormal TH serum profile, which is fully replicated in Mct8 knockout mice and Mct8/Oatp1c1 double-knockout (M/O DKO) mice. Analysis of tissue TH content and expression of TH-regulated genes indicate a thyrotoxic state in Mct8-deficient skeletal muscles. Both TH transporters are upregulated in activated satellite cells (SCs). In M/O DKO mice, we observed a strongly reduced number of differentiated SCs, suggesting an impaired stem cell function. Moreover, M/O DKO mice and mice lacking both transporters exclusively in SCs showed impaired skeletal muscle regeneration. Our data provide solid evidence for a unique gate-keeper function of MCT8 and OATP1C1 in SC activation, underscoring the importance of a finely tuned TH signaling during myogenesis. In this article, Mayerl and colleagues demonstrate that the thyroid hormone transporters MCT8 and OATP1C1 are unique gate-keepers in activated muscle stem cells. The expression of both transporters increases upon activation of muscle stem cells, while loss of MCT8 and OATP1C1 expression results in impaired muscle stem cell differentiation
- …