Appropriation in a World Heritage Site: Mozambique Island's visible and invisible complexities

Abstract: Mozambique Island, situated in the North of Mozambique, was declared a World Heritage Site by UNESCO in 1991, for its unique architecture, a testimony to the amalgamation of different cultural and scientific practices from the 16th century onwards. Generally this research is about a paradox: Mozambique Island has been recognised as a special place, and yet it is in a state of disrepair and buildings are being rebuilt and reconstructed in ways that are not consistent with the designation. Situated within the field of Human Geography, my research question Why is the World Heritage of Mozambique Island in its present state is answered through an exploration of the concept of appropriation and the importance of recognising the invisible and visible complexities of a locality. I argue that the complexities of Mozambique Island (viewed from differing scales) determine the perception of the islanders have of how World Heritage could potentially benefit them, which in turn, directly informs conservation, maintenance and management strategies (at community, city council, regional and national levels). The appropriation of identity and space only happens when the individual or group concerned think that they would benefit at some level from it. In the case of Mozambique Island, most subjects interviewed do not know what World Heritage is, how they could benefit from potential tourism associated with it and therefore why they should be putting extra effort into conserving it. Through a conversation between issues of World Heritage, identity, space, ownership, tourism and scale, I propose that in Mozambique Island there is a case of nonappropriation of its World Heritage due to its complexities, the main being: severe poverty, lack of education, bad governance, a culture of dependency and poor support from the national and regional government and UNESCO

Prevenção e controle da mastite bovina baseados no número de células somáticas.

bitstream/item/160568/1/cot-200.pd

Boas práticas agropecuárias e na produção artesanal de queijos para acessibilidade ao selo arte por agricultores familiares.

bitstream/item/221652/1/CIRC-90-20-Embrapa-Tabuleiros-Costeiros.pd

Content validation of the nursing intervention called Environmental Control: worker safety

Objective: Validating the content of the nursing intervention called Environmental Control: worker safety in Spain. Methods: An exploratory and descriptive study using the Fehring method associated with the Delphi method and a sample of 11 experts in occupational nursing. For the clarity of definition and the activities was used a scale ranging from confusing (1) and clear (7) or vaguely (1) and exactly (7). A Likert scale (1 - totally disagree /5 - totally agree) was used for the nursing action and the need of the activity for its intervention. Results: The nursing action was considered as critical with the mean score of 0.86% (SD=0.23) and 73 % of the experts considered that the title of the intervention exactly identifies the contents of the definition. Conclusion: The intervention was considered valid for occupational health with the need for practical applicability using a system of classification specific for occupational health nursing, with the Nursing Process implementation.Enfermerí

Development of a LAMP assay for detection of Leishmania infantum infection in dogs using conjunctival swab samples

Background: Leishmania infantum infections in dogs play a crucial role in the transmission of pathogens causing visceral leishmaniasis to humans in the Gansu province, northwest China. To be able to control zoonotic transmission of the parasite to humans, a non-invasive loop-mediated isothermal amplification (LAMP) assay to specifically detect L. infantum infections in dogs was developed. Methods: The primers used in the LAMP assay were designed to target kinetoplast DNA minicircle sequences of the L. infantum isolate MCAN/CN/90/SC and tested using DNA isolated from promastigotes of different Leishmania species. The LAMP assay was evaluated with conjunctional swab samples obtained from 111 and 33 dogs living in an endemic and a non-endemic region of zoonotic visceral leishmaniasis in the Gansu province, respectively. The LAMP assay was also compared with conventional PCR, ELISA and microscopy using conjunctional swab, serum and bone marrow samples from the dogs, respectively. Results: The LAMP assay detected 1 fg of L. infantum DNA purified from cultured promastigotes which was 10-fold more sensitive than a conventional PCR test using Leishmania genus-specific primers. No cross reaction was observed with DNA isolated from promastigotes of L. donovani, L. major, L. tropica, and L. braziliensis, and the L. infantum reference strain MHOM/TN/80/IPT1. The L. infantum-positive rates obtained for field-collected samples were 61.3%, 58.6%, 40.5% and 10.8% by LAMP, PCR, ELISA and microscopy, respectively. As only one out of the 33 samples from control dogs from the non-endemic region of zoonotic visceral leishmaniasis was positive by the LAMP assay and the PCR test, the observed true negative rate (specificity) was 97% for both methods. Conclusion: This study has shown that the non-invasive, conjunctional swab-based LAMP assay developed was more sensitive in the detection of leishmaniasis in dogs than PCR, ELISA and microscopy. The findings indicate that the LAMP assay is a sensitive and specific method for the field surveillance of domestic dogs, particularly of asymptomatic canines, in ZVL-endemic areas in western China

Diagnosis of Hepatozoon canis in young dogs by cytology and PCR

<p>Abstract</p> <p>Background</p> <p><it>Hepatozoon canis </it>is a widespread tick-borne protozoan affecting dogs. The diagnosis of <it>H. canis </it>infection is usually performed by cytology of blood or buffy coat smears, but this method may not be sensitive. Our study aimed to evaluate the best method to achieve a parasitological diagnosis of <it>H. canis </it>infection in a population of receptive young dogs, previously negative by cytology and exposed to tick infestation for one summer season.</p> <p>Results</p> <p>A total of 73 mongrel dogs and ten beagles younger than 18 months of age, living in an animal shelter in southern Italy where dogs are highly infested by <it>Rhipicephalus sanguineus</it>, were included in this study. In March-April 2009 and in October 2009, blood and bone marrow were sampled from each dog. Blood, buffy coat and bone marrow were examined by cytology only (at the first sampling) and also by PCR for <it>H. canis </it>(second sampling). In March-April 2009, only one dog was positive for <it>H. canis </it>by cytological examination, whereas in October 2009 (after the summer season), the overall incidence of <it>H. canis </it>infection by cytological examinations was 43.9%. Molecular tests carried out on samples taken in October 2009 showed a considerably higher number of dogs positive by PCR (from 27.7% up to 51.2% on skin and buffy coat tissues, respectively), with an overall positivity of 57.8%. All animals, but one, which were positive by cytology were also PCR-positive. PCR on blood or buffy coat detected the highest number of <it>H. canis</it>-positive dogs displaying a sensitivity of 85.7% for both tissues that increased up to 98% when used in parallel. Twenty-six (74.8%) out of the 28 <it>H. canis</it>-positive dogs presented hematological abnormalities, eosinophilia being the commonest alteration observed.</p> <p>Conclusions</p> <p>The results suggest that PCR on buffy coat and blood is the best diagnostic assay for detecting <it>H. canis </it>infection in dogs, although when PCR is not available, cytology on buffy coat should be preferred to blood smear evaluation. This study has also demonstrated that <it>H. canis </it>infection can spread among young dogs infested by <it>R. sanguineus </it>and be present in the majority of the exposed population within 6 months.</p