Comparative study of spinning field development in two species of araneophagic spiders (Araneae, Mimetidae, Australomimetus)

External studies of spider spinning fields allow us to make inferences about internal silk gland biology, including what happens to silk glands when the spider molts. Such studies often focus on adults, but juveniles can provide additional insight on spinning apparatus development and character polarity. Here we document and describe spinning fields at all stadia in two species of pirate spider (Mimetidae: Australomimetus spinosus, A. djuka). Pirate spiders nest within the ecribellate orb-building spiders (Araneoidea), but are vagrant, araneophagic members that do not build prey-capture webs. Correspondingly, they lack aggregate and flagelliform silk glands (AG, FL), specialized for forming prey-capture lines in araneoid orb webs. However, occasional possible vestiges of an AG or FL spigot, as observed in one juvenile A. spinosus specimen, are consistent with secondary loss of AG and FL. By comparing spigots from one stadium to tartipores from the next stadium, silk glands can be divided into those that are tartipore-accommodated (T-A), and thus functional during proecdysis, and those that are not (non-T-A). Though evidence was more extensive in A. spinosus, it was likely true for both species that the number of non-T-A piriform silk glands (PI) was constant (two pairs) through all stadia, while numbers of T-A PI rose incrementally. The two species differed in that A. spinosus had T-A minor ampullate and aciniform silk glands (MiA, AC) that were absent in A. djuka. First instars of A. djuka, however, appeared to retain vestiges of T-A MiA spigots, consistent with a plesiomorphic state in which T-A MiA (called secondary MiA) are present. T-A AC have not previously been observed in Australomimetus and the arrangement of their spigots on posterior lateral spinnerets was unlike that seen thus far in other mimetid genera. Though new AC and T-A PI apparently form throughout much of a spider’s ontogeny, recurring spigot/tartipore arrangements indicated that AC and PI, after functioning during one stadium, were used again in each subsequent stadium (if non-T-A) or in alternate subsequent stadia (if T-A). In A. spinosus, sexual and geographic dimorphisms involving AC were noted. Cylindrical silk gland (CY) spigots were observed in mid-to-late juvenile, as well as adult, females of both species. Their use in juveniles, however, should not be assumed and only adult CY spigots had wide openings typical of mimetids. Neither species exhibited two pairs of modified PI spigots present in some adult male mimetids

Análisis de la capacidad de soporte sísmica de fundaciones superficiales

Indexación: Web of Science; Scielo.In the context of engineering practice, the problem of the seismic bearing capacity of shallow foundations has been solved indirectly, either due an increase of the static allowable soil pressures related to the probability of occurrence of the design earthquake or by adopting an equivalent pseudo-static approach. However, during last decades, a series of analytical methods that directly address the problem from the seismic point of view has been developed. This paper presents a parametric comparative analysis of different methods for estimating seismic bearing capacity of shallow strip foundations. Analytical methods, developed in the framework of both limit equilibrium and limit analysis theories, and also simplified design procedures typically used in practice were considered. The results obtained show an important decrease of the bearing foundation capacity with increasing of the maximum earthquake acceleration, which highlights the need to obtain a measure of the reliability associated with both calculation methods and safety factors commonly used for seismic design.En el contexto de la práctica ingenieril, el problema de la capacidad de soporte sísmica de fundaciones superficiales generalmente ha sido resuelto, de modo indirecto, ya sea considerando un incremento en las tensiones admisibles estáticas del suelo, asociado a la probabilidad de ocurrencia de un cierto evento sísmico de diseño, o bien adoptando un enfoque de tipo pseudoestático equivalente. Sin embargo, durante las últimas décadas se han desarrollado una serie de métodos analíticos que abordan directamente el problema desde el punto de vista sísmico. Este artículo presenta un análisis comparativo de tipo paramétrico entre diferentes métodos para estimar la capacidad de soporte sísmica de fundaciones superficiales corridas. Se consideraron métodos analíticos, desarrollados en el contexto de las teorías de equilibrio límite y análisis límite, y también procedimientos de diseño simplificados típicamente utilizados en la práctica. Los resultados obtenidos muestran un importante deterioro de la capacidad de soporte de la fundación en la medida que la aceleración máxima del sismo aumenta, lo cual pone de manifiesto la necesidad de establecer una medida de la confiabilidad asociada a los métodos de cálculo y factores de seguridad comúnmente usados para diseño sísmico.http://ref.scielo.org/kp4j8

HPV16 E5 expression induces switching from FGFR2b to FGFR2c and epithelial-mesenchymal transition

The E5 oncoprotein of the human papillomavirus type 16 (HPV16 E5) deregulates epithelial homeostasis through the modulation of receptor tyrosine kinases and their signaling. Accordingly, the fibroblast growth factor receptor 2b (FGFR2b/KGFR), epithelial splicing transcript variant of the FGFR2, is down-modulated by the viral protein expression, leading to impairment of keratinocyte differentiation. Here, we report that, in cell models of transfected human keratinocytes as well as in cervical epithelial cells containing episomal HPV16, the down-regulation of FGFR2b induced by 16E5 is associated with the aberrant expression of the mesenchymal FGFR2c isoform as a consequence of splicing switch: in fact, quantitative RT-PCR analysis showed that this molecular event is transcriptionally regulated by the epithelial splicing regulatory proteins 1 and 2 (ESRP1 and ESRP2) and is able to produce effects synergistic with those caused by TGFβ treatment. Immunofluorescence analysis revealed that this altered FGFR2 splicing leads to changes in the specificity for the ligands FGFs and in the cellular response, triggering epithelial-mesenchymal transition (EMT). Through 16E5 or FGFR2 silencing as well as inhibition of FGFR2 activity we demonstrated the direct role of the viral protein in the receptor isoform switching and EMT, suggesting that these early molecular events during HPV infection might represent additional mechanisms driving cervical transformation and tumor progression

The influence of the preparation method of NiOx photocathodes on the efficiency of p-type dye-sensitised solar cells

Improving the efficiency of p-type dye-sensitized solar cells (DSCs) is an important part of the development of high performance tandem DSCs. The optimization of the conversion efficiency of p-DSCs could make a considerable contribution in the improvement of solar cells at a molecular level. Nickel oxide is the most widely used material in p-DSCs, due to its ease of preparation, chemical and structural stability, and electrical properties. However, improvement of the quality and conductivity of NiO based photocathodes needs to be achieved to bring further improvements to the solar cell efficiency. The subject of this review is to consider the effect of the preparation of NiO surfaces on their efficiency as photocathodes. (C) 2015 Elsevier B.V. All rights reserved

Maars to calderas. End-members on a spectrum of explosive volcanic depressions

We discuss maar-diatremes and calderas as end-members on a spectrum of negative volcanic landforms (depressions) produced by explosive eruptions (note—we focus on calderas formed during explosive eruptions, recognizing that some caldera types are not related to such activity). The former are dominated by ejection of material during numerous discrete phreatomagmatic explosions, brecciation, and subsidence of diatreme fill, while the latter are dominated by subsidence over a partly evacuated magma chamber during sustained, magmatic volatile-driven discharge. Many examples share characteristics of both, including landforms that are identified as maars but preserve deposits from non-phreatomagmatic explosive activity, and ambiguous structures that appear to be coalesced maars but that also produced sustained explosive eruptions with likely magma reservoir subsidence. A convergence of research directions on issues related to magma-water interaction and shallow reservoir mechanics is an important avenue toward developing a unified picture of the maar-diatreme-caldera spectrum

How far do shocks move across borders?: Examining volatility transmission in major agricultural futures markets

This paper examines the level of interdependence and volatility transmission across major exchanges of maize, wheat, and soybeans in the United States, Europe, and Asia. We follow a multivariate GARCH approach to explore in detail and under different specifications the dynamics and cross-dynamics of volatility in agricultural futures markets. We account for the potential bias that may arise when considering exchanges with different closing times. The period of analysis is 2004–2009 for maize and soybeans and 2005–2009 for wheat. The results indicate that there is a strong correlation among international markets. In particular, we find both own- and cross-volatility spillovers and dependence between most of the exchanges. There is also higher interaction between the United States (Chicago) and both Europe and Asia than within the latter. The results further show the major role Chicago plays in terms of spillover effects over the other markets, particularly for maize and wheat. For soybeans, both China and Japan also exhibit important cross-volatility spillovers. Finally, the level of interdependence between exchanges has not necessarily increased in recent years for all commodities. From a policy perspective, these findings suggest that any potential regulatory scheme to address (excessive) price volatility in agricultural exchanges should be coordinated across markets; localized regulation will have limited effects given the high level of interrelation between markets.volatility transmission, agricultural commodities, futures markets, multivariate GARCH,

Assessing the impact of the SASA/CASREN technology interventions in the sweet potato-pig production systems in Zitong County (Sichuan, China)

The present paper describes the processes applied by the Sichuan Animal Science Academy (SASA)/CASREN Team in Zitong County in order to improve the sweet potato-based pig production systems that are predominantly present in that area, which is representative of the rainfed uplands of Sichuan Province (China). The methodology applied by the CASREN project to scale up promising technology interventions in croplivestock systems is described. Some of the differences between the work in the benchmark site in Sichuan Province and other CASREN sites is also briefly described. The last part of the report contains a detailed description of the basket of technology op tions proposed by the SASA/CASREN Team, as well as some of the findings in the preliminary assessment of their impact. The technologies included in the basket of options are: improved sweet potato varieties (high yielding, starch-rich); conservation of sweet potato roots and vines as silages as a means to increase the efficiency of utilisation of sweet potatoes as animal feed; use of a premix or a protein-rich concentrate to supplement sweet potato-based diets; upgrading the local pig genotypes using improved breeds; implementation of a simple preventive disease control program; and improved housing. The report contains some of the preliminary results obtained applying a set of criteria for assessing the impacts at the plot and household level, after applying the technology interventions as proposed by the SASA/CASREN Team. Among those criteria are: increased sweet potato yield; larger area planted to the improved varieties; reduction of losses due spoilage when sweet potato roots and vines are ensiled; savings in fuel when silages are prepared instead of offering fresh roots; shorten the growing/fattening period; increased numbers of pigs marketed; increased income from pig; reduced costs of feeding; faster capital turnover in the pig enterprise; and final ly improved livelihood for household members. At the end of the report there is a brief section on institutional impacts