The role of radiotherapy in the complex treatment of mycosis fungoides; a case report

Introduction. Mycosis fungoides (MF) is the most common form of primary cutaneous T-cell lymphoma. Radiotherapy induces excellent response rates in the treatment of localized or extensive lesions of MF, both as monotherapy and as part of a multidisciplinary treatment. Total skin irradiation with electrons or protons is available in a limited number of medical centers and is mainly used in patients with extensive MF. Case presentation. It is presented the case of a 47-year-old patient who developed erythematous papules and plaques on the chest, upper limbs, face and scalp, initially diagnosed as chronic eczema and treated with systemic corticotherapy, antihistamines and dupilumab. Under treatment, the lesions progressed with the appearance of tumorous formations on the scalp and face, some of them ulcerated. Thus, additional (histopathological and immunohistochemical) tests were performed and established the diagnosis of MF. As a result, treatment with methotrexate and narrow-band UVB was initiated, subsequently associated with radiotherapy on the scalp and face lesions (right frontal, right parietal, left oral commissure), 30 Gy to each area. During radiotherapy, the patient developed second grade radiodermatitis associated with radiomucositis, but with a significant reduction in the surface area of the lesions until their disappearance. Conclusions. Radiotherapy can be used in all stages of MF, being useful both for alleviating symptoms and eradicating the disease, meaning that the potential of this form of treatment needs to be further exploited and investigated

Economic advantages of using bacterial biopreparations in agricultural crops

The ecological, genetic, biological approach proposed by agricultural specialists in order to protect plants and crops has a role in reducing the impact of pests through the process of selection and improvement of genetic resources in the processes of planting, development and introduction of biological means to combat pests in agricultural ecosystems. The strategies proposed by the specialists in the agricultural field aim not at the total extermination of the pests from the agricultural crops but at keeping the pest populations at the optimal damage threshold. The most important advantages of these biological processes are those of the evolutionary stability of the crop systems, the ecological stabilization of the pest and crop populations as well as the assurance of a superior quality of the resulting agricultural products.The present paper aims to present the main advantages of using bacterial biopreparations in agricultural ecosystems (research conducted in agricultural research stations in Romania), reducing soil pollution, environmental crops, use of alternative fertilization and cultivation technologies as well as obtaining additional, ecological productions.The aim of this paper is to present the economic advantages of using bacterial biopreparations in agricultural research and development stations, reducing costs in agriculture and the processes that these bacterial biopreparations have on the agricultural ecosystem, the environment and humans and animals

Large-scale mitochondrial DNA analysis reveals new light on the phylogeography of Central and Eastern-European Brown hare (Lepus europaeus Pallas, 1778)

European brown hare, Lepus europaeus, from Central and Eastern European countries (Hungary, Poland, Serbia, Lithuania, Romania, Georgia and Italy) were sampled, and phylogenetic analyses were carried out on two datasets: 1.) 137 sequences (358 bp) of control region mtDNA; and 2.) 105 sequences of a concatenated fragment (916 bp), including the cytochrome b, tRNA-Thr, tRNA-Pro and control region mitochondrial DNA. Our sequences were aligned with additional brown hare sequences from GenBank. A total of 52 and 51 haplotypes were detected within the two datasets, respectively, and assigned to two previously described major lineages: Anatolian/Middle Eastern (AME) and European (EUR). Furthermore, the European lineage was divided into two subclades including South Eastern European (SEE) and Central European (CE). Sympatric distribution of the lineages of the brown hare in South-Eastern and Eastern Europe revealed contact zones there. BAPS analysis assigned sequences from L. europaeus to five genetic clusters, whereas CE individuals were assigned to only one cluster, and AME and SEE sequences were each assigned to two clusters. Our findings uncover numerous novel haplotypes of Anatolian/Middle Eastern brown hare outside their main range, as evidence for the combined influence of Late Pleistocene climatic fluctuations and anthropogenic activities in shaping the phylogeographic structure of the species. Our results support the hypothesis of a postglacial brown hare expansion from Anatolia and the Balkan Peninsula to Central and Eastern Europe, and suggest some slight introgression of individual haplotypes from L. timidus to L. europaeus

Peri-operative red blood cell transfusion in neonates and infants: NEonate and Children audiT of Anaesthesia pRactice IN Europe: A prospective European multicentre observational study

BACKGROUND: Little is known about current clinical practice concerning peri-operative red blood cell transfusion in neonates and small infants. Guidelines suggest transfusions based on haemoglobin thresholds ranging from 8.5 to 12 g dl-1, distinguishing between children from birth to day 7 (week 1), from day 8 to day 14 (week 2) or from day 15 (≥week 3) onwards. OBJECTIVE: To observe peri-operative red blood cell transfusion practice according to guidelines in relation to patient outcome. DESIGN: A multicentre observational study. SETTING: The NEonate-Children sTudy of Anaesthesia pRactice IN Europe (NECTARINE) trial recruited patients up to 60 weeks' postmenstrual age undergoing anaesthesia for surgical or diagnostic procedures from 165 centres in 31 European countries between March 2016 and January 2017. PATIENTS: The data included 5609 patients undergoing 6542 procedures. Inclusion criteria was a peri-operative red blood cell transfusion. MAIN OUTCOME MEASURES: The primary endpoint was the haemoglobin level triggering a transfusion for neonates in week 1, week 2 and week 3. Secondary endpoints were transfusion volumes, 'delta haemoglobin' (preprocedure - transfusion-triggering) and 30-day and 90-day morbidity and mortality. RESULTS: Peri-operative red blood cell transfusions were recorded during 447 procedures (6.9%). The median haemoglobin levels triggering a transfusion were 9.6 [IQR 8.7 to 10.9] g dl-1 for neonates in week 1, 9.6 [7.7 to 10.4] g dl-1 in week 2 and 8.0 [7.3 to 9.0] g dl-1 in week 3. The median transfusion volume was 17.1 [11.1 to 26.4] ml kg-1 with a median delta haemoglobin of 1.8 [0.0 to 3.6] g dl-1. Thirty-day morbidity was 47.8% with an overall mortality of 11.3%. CONCLUSIONS: Results indicate lower transfusion-triggering haemoglobin thresholds in clinical practice than suggested by current guidelines. The high morbidity and mortality of this NECTARINE sub-cohort calls for investigative action and evidence-based guidelines addressing peri-operative red blood cell transfusions strategies. TRIAL REGISTRATION: ClinicalTrials.gov, identifier: NCT02350348

Preliminary Report on CSN3 and LGB genes Polymorphism Among Two Romanian Cattle Breeds

The aim of the current study was to evaluate the genetic polymorphism of A and B variants at the CSN3 (κ-casein) and LGB (β-lactoglobulin) loci in Romanian Spotted and Romanian Brown breeds using PCR-RFLP and PCR-HRM genotyping methods. Altogether, 280 cattle from Arad county were genotyped. The most frequent allele at the CSN3 locus was A in Romanian Spotted breed (0.723) while the B allele was the most common in the Romanian Brown (0.694). For LGB locus the A and B alleles recorded similar frequencies for Romanian Spotted (0.5141 and 0.4859) compared to Romanian Brown breed where the B allele was the most prevalent (0.5970). Knowledges regarding genetic information of breeds related to milk protein are valuable for economic reassertion of cattle breeds, particularly autochthonous ones. Further, the results would be useful in future breeding programs for the studied population, aimed to improve cattle genetic structure for milk quality traits

Strategies for Diagnosis of Diseases in Pigs Using Molecular Markers Review

In certain breeds of pigs were identified the porcine stress syndrome (PSS) that determine the appearance of some carcasses inadequate for processing. Because PSS can be triggered by halothane, the gene responsible for the syndrome is often referred to as the “halothane gene” (Hal). The metabolic and physiological changes that occur in halothane positive pigs (homozygous recessive "nn") are produced by gene located in the Hal locus responsible for synthesis of the Ca2 + channel receptor in the sarcoplasmatic reticulum of skeletal muscle fiber, called Ryanodin receptor (RYR1). The Ryanodin receptor locus in pig populations is important not only for economic losses caused by homozygous recessive pigs, but also for the fact that this locus is linked to other quantitatively additive genes which determine muscle hypertrophy. The unconscious promotion of carrier and positive animals in the herd, due to the intention to produce a new generation characterized by muscle hypertrophy, led to the automatic increase of frequency of the mutant allele "n" in the pig population. PCR assay of porcine genetic background can help determine with great precision the frequency of specific alleles in the RYR1 locus, offering the specialists a possibility of reducing the recessive allele frequency through selection. Therefore, the present review underlines the necessity of implementing such testing programs in Romania in order to prevent the risk of dissemination of PSS in pigs

Factors Affecting Calves Suckling Behavior

The aim of paper was to assess effects of environmental and physiological related factors on suckling behavior patterns. The study was carried out on 120 Romanian Spotted calves. Two behavioral traits were considered: total daily required interval and required interval for each meal. Traits were studied according to: calves viability, calving season, type of calving, calving ease and meal sequence. Significant differences were recorded for calves viability (viable vs morbid, p≤0.001), type of calving (single vs twinning, p≤0.001), and calving ease (eutocya vs dystocia, p≤0.001), being a proof of welfare, conferred by adaptability to new environment. No significant effect was recorded for season of calving (summer vs winter, p≥0.05). Significant differences concerning required suckling interval, were recorded between meals, both seasons. for viable (p≤0.001), morbid (p≤0.05), singles (p≤0.05) and calves born from eutocya births (p≤0.001). No significant differences (p≥0.05) recorded for twins and calves born from dystocia. Multifactorial regression model used, reveals that main effects on studied traits had viability (p≤0.001), type of calving (p≤0.01) and calving ease (p≤0.05). It is reasonable to assume that the knowledge of suckling behavior contributes to optimal management of calves. Knowledge of these factors helps to develop adequately systems to improve calves suckling response

Comparative study on production, reproduction and functional traits between Fleckvieh and Braunvieh cattle

Objective Aim of the current comparative study was to evaluate production outputs, reproduction efficiency and functional traits in dual-purpose Fleckvieh and Braunvieh cows, reared under temperate European conditions. Methods A data-set from 414 Fleckvieh and 42 Braunvieh cows and 799 lactations was analysed. ID tag number, milk yield per milking session, number of steps/interval and milk conductivity were recorded and collected daily using AfiMilk 3.076 A-DU software (Afimilk Ltd., Kibbutz, Israel). Production and milk quality data were taken from the results of the official performance recordings and the reproductive outputs of cows were recorded by the research stations veterinarians. Comparisons between the two genotypes were carried out using the one way analysis of variance protocol, with categorical factor being considered the breed of cows. All the statistical inferences were carried out using Statistica software (StatSoft Inc., Tulsa, OK, USA). Results Fleckvieh cows significantly outperformed (p≤0.05) the Braunvieh herd, with average milk yields of 5,252.1±35.79 kg and 4,897.6±128.94 kg, respectively. Age at first calving was significantly (p≤0.01) influenced by the breed, with Fleckvieh heifers being more precocious (32.8±0.29 mo) compared to those of Braunvieh breed (35.7±0.84 mo). Reproduction efficiency as defined by the number of inseminations per gestation, calving interval, dystocia, days dry and days open, was not influenced by genotype (p>0.05). Incidences of sub-clinical mastitis, clinical mastitis, lameness and abortions were not influenced by the breed factor (p>0.05). Stayability of cows was significantly (p≤0.001) influenced by genotype, with Braunvieh cows having an average age at culling of 117.88±11.78 months compared to 90.88±2.89 months in Fleckvieh. Conclusion Overall, results have shown that genotype significantly influenced milk yield, age at first calving and longevity