Vanadate and bone metabolism: effect on proliferation and mineralization of fish bone-derived cells

Vanadate is known for mimicking insulin action through activation of insulin and/or insulin like growth factor 1 (IGF 1) receptors. Vanadate insulin- like effect on bone-related metabolism has been previously investigated using mammalian in vitro cell systems but other vertebrate systems have rarely been used. We have recently demonstrated the suitability of a fish bone derived cell line (VSa13) to study anti-mineralogenic effects of vanadate. Here, we propose that vanadate stimulation of cell proliferation involves MAPK signalling pathway and IGF 1 receptor activation, while impairment of extracellular matrix (ECM) mineralization is likely to involve both MAPK and PI 3K pathways and insulin receptor activation

Vanadate effects on bone metabolism: fish cell lines as an alternative to mammalian in vitro systems

Vanadate, one of the most relevant forms of vanadium in solution, has been associated with the regulation of various enzyme activities (e.g. phosphatases, ribonucleases, ATPases, etc.) and shown to exhibit important biological effects. Several in vivo and in vitro studies have clearly demonstrated that any deficiency or excess of vanadium can seriously affect bone formation and its metabolism. Bone-related effects result largely from vanadium insulino-mimetic capabilities mediated by specific inhibition of protein tyrosine phosphatases (PTPases) and consequent activation of tyrosine kinase receptors (e.g. insulin receptor). Although mammals have been repetitively shown to be appropriate models to study vanadate mechanisms of action, fish have recently emerged as alternative models. Fish has been recognized as suitable model to study vertebrate bone formation and the natural presence of high quantities of vanadium in water makes it even more suitable to investigate vanadium effect on bone formation. Recent data obtained using fish bone-derived cells revealed that micromolar concentrations (5 mM) of monomeric and decameric vanadate slightly stimulate growth performances while strongly inhibiting extracellular matrix mineralization through mechanisms involving both alkaline phosphatase and MAPK pathways. Recent data obtained in fish cells will be discussed here and further compared to results obtained in mammalian systems

Impairment of mineralization by metavanadate and decavanadate solutions in a fish bone-derived cell line

Vanadium, a trace metal known to accumulate in bone and to mimic insulin, has been shown to regulate mammalian bone formation using in vitro and in vivo systems. In the present work, short- and long-term effects of metavanadate (containing monomeric, dimeric, tetrameric and pentameric vanadate species) and decavanadate (containing decameric vanadate species) solutions on the mineralization of a fish bone-derived cell line (VSa13) were studied and compared to that of insulin. After 2 h of incubation with vanadate (10 μM in monomeric vanadate), metavanadate exhibited higher accumulation rates than decavanadate (6.85±0.40 versus 3.95±0.10 μg V/g of protein, respectively) in fish VSa13 cells and was also shown to be less toxic when applied for short periods. In longer treatments with both metavanadate and decavanadate solutions, similar effects were promoted: stimulation of cell proliferation and strong impairment (75%) of extracellular matrix (ECM) mineralization. The effect of both vanadate solutions (5 μM in monomeric vanadate), on ECM mineralization was increased in the presence of insulin (10 nM). It is concluded that chronic treatment with both vanadate solutions stimulated fish VSa13 cells proliferation and prevented ECM mineralization. Newly developed VSa13 fish cells appeared to be appropriate in the characterization of vanadate effects on vertebrate bone formation, representing a good alternative to mammalian systems

Technologic Appliance and Performance Concerns in Wheelchair Racing – Helping Paralympic Athletes to Excel

Numerical simulations have provided useful evidence in helping several sportsmen to excel in their field. This methodology aims to have a deeper understanding on the influence of equipment and sports techniques on sports performance. In wheelchair racing, technology was used without considering specific sport (some of the Paralympic sports used the same technology of their Olympic counterparts). It has induced unique changes in prosthetic and wheelchair devices. Eventually, technology has become an essential part of Paralympic sports, wheelchair-racing being one of the most popular events. Numerical simulations can help us gather evidence on the effects of drag force acting upon the athlete-chair system

Complementing Warm-up with Stretching Routines: Effects in Sprint Performance

The present study aimed to examine the effects of using static or dynamic stretching added to the common warm-up routine for short sprint distances and to repeated sprint performance. In 3 different sessions, 16 college-age men (n = 10) and women (n = 6) performed one of 3 warm-ups followed by a 2 × 60 m dash sprint time trial (5 min of rest) in a counterbalanced design. The control warm-up consisted of 10 min of light-intensity running, and the 2 experimental warm-ups included a static or dynamic stretching routine (5 exercises) in the control warm-up. Performance (time) and physiological variables (tympanic temperature, heart rate) were monitored. In the first 60 m time trial, there were no differences between the 3 warmups tested (F = 0.21, p = 0.73; ηp 2 = 0.01), as opposed to that observed in the second (F = 7.04, p < 0.01; ηp 2 = 0.32). The participants were 1.7 % faster after the static stretching warm-up compared with the control warm-up. The sum of the time performed in the 2 sprints emphasizes these results, with better performances after the static stretching warm-up than the control (1 %) or dynamic stretching warm-up (0.7 %). These results suggest that including a set of static or dynamic stretching exercises may enhance sprinting performance. The better performance in the second trial after the warm-up including static stretching suggests that this type of stretching may positively influence repeated sprint performance ( < 10 s sprint).info:eu-repo/semantics/publishedVersio

Warm-up and performance in competitive swimming

Warm-up before physical activity is commonly accepted to be fundamental, and any priming practices are usually thought to optimize performance. However, specifically in swimming, studies on the effects of warm-up are scarce, which may be due to the swimming pool environment, which has a high temperature and humidity, and to the complexity of warm-up procedures. The purpose of this study is to review and summarize the different studies on how warming up affects swimming performance, and to develop recommendations for improving the efficiency of warm-up before competition. Most of the main proposed effects of warm-up, such as elevated core and muscular temperatures, increased blood flow and oxygen delivery to muscle cells and higher efficiency of muscle contractions, support the hypothesis that warm-up enhances performance. However, while many researchers have reported improvements in performance after warm-up, others have found no benefits to warm-up. This lack of consensus emphasizes the need to evaluate the real effects of warm-up and optimize its design. Little is known about the effectiveness of warm-up in competitive swimming, and the variety of warm-up methods and swimming events studied makes it difficult to compare the published conclusions about the role of warm-up in swimming. Recent findings have shown that warm-up has a positive effect on the swimmer's performance, especially for distances greater than 200 m. We recommend that swimmers warm-up for a relatively moderate distance (between 1,000 and 1,500 m) with a proper intensity (a brief approach to race pace velocity) and recovery time sufficient to prevent the early onset of fatigue and to allow the restoration of energy reserves (8-20 min).UBI/FCSH/Santander/2010info:eu-repo/semantics/publishedVersio

Physiological assessment of head-out aquatic exercises in healthy subjects: a qualitative review

In the last decades head-out aquatic exercises became one of the most important physical activities within the health system. Massive research has been produced throughout these decades in order to better understand the role of head-out aquatic exercises in populations' health. Such studies aimed to obtain comprehensive knowledge about the acute and chronic response of subjects performing head-out aquatic exercises. For that, it is assumed that chronic adaptations represent the accumulation of acute responses during each aquatic session. The purpose of this study was to describe the "state of the art "about physiological assessment of head-out aquatic exercises based on acute and chronic adaptations in healthy subjects based on a qualitative review. The main findings about acute response of head-out aquatic exercise according to water temperature, water depth, type of exercise, additional equipment used, body segments exercising and music cadence will be described. In what concerns chronic adaptations, the main results related to cardiovascular and metabolic adaptations, muscular strength, flexibility and body composition improvements will be reported. Key pointsSeveral papers reported consistent and significant improvement in physical fitness (e.g., aerobic capacity, muscular strength, flexibility and body composition) after a program of head-out aquatic exercise with at least eight weeks.Chronic adaptations to head-out aquatic exercise programs are the cumulative result of appropriate acute responses during the exercise session.Appropriate acute adaptations can be obtained taking into account the water temperature, water depth, type of exercise and its variants, the equipment used and the segmental cadence according to the subjects' profile.info:eu-repo/semantics/publishedVersio

Biomechanics of Competitive Swimming Strokes

The aim of this chapter has two folds: (i): to perform a biomechanical characterization of the four competitive swimming strokes, based on the kinematics, kinetics and neuromuscular analysis; (ii) to report the relationships established between all the domains and how it might influence the swimming performance