Vanadate is known for mimicking insulin action through activation of insulin and/or insulin like growth factor 1 (IGF 1) receptors. Vanadate insulin-
like effect on bone-related metabolism has been previously investigated using mammalian in vitro cell systems but other vertebrate systems have rarely been used. We have recently demonstrated the suitability of a fish bone derived cell line (VSa13) to study anti-mineralogenic effects of vanadate.
Here, we propose that vanadate stimulation of cell proliferation involves MAPK
signalling pathway and IGF 1 receptor activation, while impairment of extracellular
matrix (ECM) mineralization is likely to involve both MAPK and PI 3K pathways and insulin receptor activation
