3 research outputs found
Sa1388 Symptom Recurrence After Stopping Proton Pump Inhibitors (PPIs) in Patients Undergoing Esophageal pH Monitoring: “the Reverse Therapeutic PPI Trial”
Revisiting Coley’s Toxins: Immunogenic Cardiolipins from <i>Streptococcus pyogenes</i>
Coley’s toxins, an early and enigmatic form of
cancer (immuno)therapy,
were based on preparations of Streptococcus pyogenes. As part of a program to explore bacterial metabolites with immunomodulatory
potential, S. pyogenes metabolites
were assayed in a cell-based immune assay, and a single membrane lipid,
18:1/18:0/18:1/18:0 cardiolipin, was identified. Its activity was
profiled in additional cellular assays, which showed it to be an agonist
of a TLR2–TLR1 signaling pathway with a 6 μM EC50 and robust TNF-α induction. A synthetic analog with switched
acyl chains had no measurable activity in immune assays. The identification
of a single immunogenic cardiolipin with a restricted structure–activity
profile has implications for immune regulation, cancer immunotherapy,
and poststreptococcal autoimmune diseases