172 research outputs found

    EFFECT OF POPULATION TYPE ON THE DEFINITION OF BREEDING GOALS

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    Moose, trees, and ground-living invertebrates: indirect interactions in Swedish pine forests

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    The role of moose in structuring the boreal forest ecosystem has been studied extensively in recent pears. This research has focused mainly on the effects of moose on vegetation and soil dynamics. However, the extent to which these effects influence animal communities has received little attention. We studied how invertebrate assemblages on the forest floor of two Swedish pine forests were affected by the presence of moose. Our approach was to trap invertebrates using pitfall traps in unbrowsed exclosures and browsed reference plots, estimate the percentage cover of herb-layer plants, measure the height and density of tree species, and measure the accumulation of leaf litter. The effect of moose on tree strata differed between the two areas. In Sunnas, where the stand was a mixture of deciduous trees and pines, the main effect of moose was to eliminate most of the deciduous trees from the canopy. Here the amount of deciduous leaf litter was ten times higher in unbrowsed exclosures compared with browsed plots. The stands on the dry sites in the other area, Furudal, were almost pure pine. Here, moose browsing reduced the density of taller pines, but increased the number of pine saplings. At Sunnas, the herb-layer plant cover did not differ between browsed and unbrowsed plots. However, there was a difference between exclosures and browsed plots in terms of the ground-dwelling invertebrate assemblage. Number of individuals of most invertebrate groups that differed between exclosures and browsed plots were higher in unbrowsed plots and the total number of invertebrates was also higher in unbrowsed plots, but diversity of invertebrate fauna was higher in browsed plots. At Furudal both the vegetation and invertebrate assemblages differed between browsed and unbrowsed plots. Browsed plots had a higher cover of ground lichens; while unbrowsed plots had more mosses. Among the invertebrate taxa that differed between treatments, the number favored by browsing and the number disfavored by browsing were roughly equal, and diversity was not affected by moose. We suggest that by affecting the density and composition of tree species, moose indirectly influenced the structure of invertebrate communities. At Sunnas, the reduction in the amount of deciduous leaf litter was probably the key effect. Vegetation was not affected much, but the differences in litter layer composition had affected the microclimate on the forest floor while also providing an important additional trophic resource and habitat for ground-dwelling invertebrates. Al Furudal the main effect of moose was to reduce the density of the pine canopy: thus increasing the amount of light penetrating to the forest floor. As a consequence, the floral composition and microclimate was altered which, in turn, affected the invertebrate fauna

    Underestimated role of legume roots for soil N fertility

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    Research ArticleNitrogen (N) is a major fertilizing element for plants. The distribution of N in legumes is influencing the efficiency of the next crop. Nitrogen storage in legumes is actually estimated by N fixation in shoots, whereas there is little knowledge on the contribution of roots and nodules to legume N and soil N. Here, we studied the contribution of roots and nodules of grain and pasture legumes to plant N and soil N in Mediterranean fields. Experiments were run under rainfed conditions for a 2-year period in three regions of Portugal. Entire plants including top plant and visible roots and nodules were sampled at the end of the growing seasons for grain legumes, sweet and yellow lupine, and over two harvests in case of pastures. N2 fixation was measured for grain legumes and pasture legumes using 15N tracing. Our results show that aboveground N concentration did not vary among legumes, but differed in the belowground tissues. Field studies show that 7–11%of total legume N was associated with roots and nodules. Data also show an allocation of 11– 14 kg N fixed t−1 belowground dry matter in indeterminate legumes, which represents half the amount of total aboveground plant. This finding demonstrates that investigation relying only on shoot Nunderestimates the role of legumes for soil N fertilityinfo:eu-repo/semantics/publishedVersio

    Towards high-throughput metabolomics using ultrahigh-field Fourier transform ion cyclotron resonance mass spectrometry

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    With unmatched mass resolution, mass accuracy, and exceptional detection sensitivity, Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FTICR-MS) has the potential to be a powerful new technique for high-throughput metabolomic analysis. In this study, we examine the properties of an ultrahigh-field 12-Tesla (12T) FTICR-MS for the identification and absolute quantitation of human plasma metabolites, and for the untargeted metabolic fingerprinting of inbred-strain mouse serum by direct infusion (DI). Using internal mass calibration (mass error ≤1 ppm), we determined the rational elemental compositions (incorporating unlimited C, H, N and O, and a maximum of two S, three P, two Na, and one K per formula) of approximately 250 out of 570 metabolite features detected in a 3-min infusion analysis of aqueous extract of human plasma, and were able to identify more than 100 metabolites. Using isotopically-labeled internal standards, we were able to obtain excellent calibration curves for the absolute quantitation of choline with sub-pmol sensitivity, using 500 times less sample than previous LC/MS analyses. Under optimized serum dilution conditions, chemical compounds spiked into mouse serum as metabolite mimics showed a linear response over a 600-fold concentration range. DI/FTICR-MS analysis of serum from 26 mice from 2 inbred strains, with and without acute trichloroethylene (TCE) treatment, gave a relative standard deviation (RSD) of 4.5%. Finally, we extended this method to the metabolomic fingerprinting of serum samples from 49 mice from 5 inbred strains involved in an acute alcohol toxicity study, using both positive and negative electrospray ionization (ESI). Using these samples, we demonstrated the utility of this method for high-throughput metabolomics, with more than 400 metabolites profiled in only 24 h. Our experiments demonstrate that DI/FTICR-MS is well-suited for high-throughput metabolomic analysis

    Can forest management based on natural disturbances maintain ecological resilience?

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    Given the increasingly global stresses on forests, many ecologists argue that managers must maintain ecological resilience: the capacity of ecosystems to absorb disturbances without undergoing fundamental change. In this review we ask: Can the emerging paradigm of natural-disturbance-based management (NDBM) maintain ecological resilience in managed forests? Applying resilience theory requires careful articulation of the ecosystem state under consideration, the disturbances and stresses that affect the persistence of possible alternative states, and the spatial and temporal scales of management relevance. Implementing NDBM while maintaining resilience means recognizing that (i) biodiversity is important for long-term ecosystem persistence, (ii) natural disturbances play a critical role as a generator of structural and compositional heterogeneity at multiple scales, and (iii) traditional management tends to produce forests more homogeneous than those disturbed naturally and increases the likelihood of unexpected catastrophic change by constraining variation of key environmental processes. NDBM may maintain resilience if silvicultural strategies retain the structures and processes that perpetuate desired states while reducing those that enhance resilience of undesirable states. Such strategies require an understanding of harvesting impacts on slow ecosystem processes, such as seed-bank or nutrient dynamics, which in the long term can lead to ecological surprises by altering the forest's capacity to reorganize after disturbance

    Boom and bust of a moose population – a call for integrated forest management

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    This is the postprint version of the article. The published article can be located at www.springerlink.comThere is increasing pressure to manage forests for multiple objectives, including ecosystem services and biodiversity, alongside timber production. However, few forests are currently co-managed for timber and wildlife, despite potential economic and conservation benefits. We present empirical data from a commercial Norway spruce ( Picea abies ) and Scots pine ( Pinus sylvestris ) production system in southern Norway in which moose ( Alces alces ) are an important secondary product. Combining long-term hunting and forestry records, we identified temporal vari- ation in clear-felling over the past five decades, peaking in the 1970s. Herbicide treatment of regenerating stands and a fivefold increase in moose harvest has lead to a reduction in availability of successional forest per moose of [ 90 % since the 1960s. Field estimates showed that spraying with the herbicide glyphosate reduced forage availability by 60 and 96 % in summer and winter, respectively, 4 years after treatment. It also reduced moose use and habitat selection of young spruce stands compared with unsprayed stands. Together these lines of evidence suggest that forest man- agement led to an increase in moose carrying capacity during the 1970s and a subsequent decline thereafter. This is likely to have contributed to observed reductions in moose population productivity in southern Norway and is counter to sustainable resource management. We therefore call for better integration and long-term planning between forestry and wildlife management to minimise forest damage and the development of large fluctuations in ungulate populations
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