8 research outputs found
Adopce a jejà význam v prevenci psychické deprivace
Sputum basophil gating strategy and change in marker expression. Representative sputum dot plots showing basophil gating strategy used for flow cytometry analysis including: (A) Side Scatter (SSC) vs. Forward Side Scatter (FSC), (B) SSC vs. CD45 Alexa Fluor 700, (C) SSC vs. HLA-DR APCH7 and (D) SSC vs. IL-3RĂŽÄ… PECy7. Panels E-G are representative dot plots of the change in the percentage of basophils expressing IL-17RB, ST2, and intracellular IL-25 at pre-allergen challenge (blue), 7Ă‚Â h post-allergen (green) and 24Ă‚Â h post-allergen challenge (orange), compared to the isotype control (red). (TIFF 10198 kb
Additional file 5: of The association between airway eosinophilic inflammation and IL-33 in stable non-atopic COPD
Figure E2. Circulating (A) and sputum (B) hemopoietic progenitor cells (HPC) in patients with and without sputum eosinophilia. **p < 0.01. (PDF 56 kb
Additional file 6: Figure S6. of IL-25 and IL-33 induce Type 2 inflammation in basophils from subjects with allergic asthma
Basophil activation marker dose response to IL-25. Isolated peripheral blood basophils from 8 allergic subjects were suspended in RPMI-C and cultured for 18 h with PBS (negative control, open bars) or increasing concentrations of IL-25 (0.1, 1, 10, 100 ng/mL) (black bars), were assessed for expression of (A) CD203c, (B) IL-3Rα, (C) CCR3, (D) intracellular IL-13, (E) intracellular IL-4, (F) intracellular IL-25, (G) IL-17RB, (H) ST2, and (I) TSLPR. Data expressed as mean ± SEM. *Significantly different from PBS negative control; P < 0.05 (N = 8). (TIFF 2740 kb
Additional file 1: Figure S1. of IL-25 and IL-33 induce Type 2 inflammation in basophils from subjects with allergic asthma
Bone marrow basophil gating strategy and change in marker expression. Representative bone marrow dot plots showing basophil gating strategy used for flow cytometry analysis including: (A) Side Scatter (SSC) vs. Forward Side Scatter (FSC), (B) SSC vs. CD45 Alexa Fluor 700, (C) SSC vs. HLA-DR APCH7 and (D) SSC vs. IL-3RĂŽÄ… PECy7. Panels E-G are representative dot plots of the change in the percentage of basophils expressing IL-17RB, ST2, and intracellular IL-25 at pre-allergen challenge (blue) and 24Ă‚Â h post-allergen challenge (green), compared to the isotype control (red). (TIFF 10587 kb
Additional file 4: of The association between airway eosinophilic inflammation and IL-33 in stable non-atopic COPD
Table E1. Correlations between serum and sputum IL-33 and sST2, ST2 mRNA and clinical parameters in COPD. FEV1 – forced expiratory volume in first second; FVC – forced vital capacity; CAT – COPD assessment test; 6MWT – six-minute walk test; mMRC – modified Medical Research Council dyspnea scale. (PDF 39 kb
Additional file 1: of The association between airway eosinophilic inflammation and IL-33 in stable non-atopic COPD
Detailed Method description. (PDF 118Â kb
Additional file 5: Figure S5. of IL-25 and IL-33 induce Type 2 inflammation in basophils from subjects with allergic asthma
Basophil activation marker dose response to IL-33. Isolated peripheral blood basophils from 8 allergic subjects were suspended in RPMI-C and cultured for 18 h with PBS (negative control, open bars) or increasing concentrations of IL-33 (0.1, 1, 10, 100 ng/mL) (black bars), were assessed for expression of (A) CD203c, (B) IL-3Rα, (C) CCR3, (D) intracellular IL-13, (E) intracellular IL-4, (F) intracellular IL-25, (G) IL-17RB, (H) ST2, and (I) TSLPR. Data expressed as mean ± SEM. *Significantly different from PBS negative control; P < 0.05 (N = 8). (TIFF 2756 kb
Additional file 2: of The association between airway eosinophilic inflammation and IL-33 in stable non-atopic COPD
Figure E4. Hemopoietic progenitor cells gating strategy. (PDF 88Â kb