Editorial : Vaccination strategies against ruminant infectious diseases

Ruminant infectious diseases cause economic impact through losses in animal production and human health. Most of the commercially available veterinary vaccines are live attenuated or inactivated which induce different degrees of efficacy, i.e., decrease in clinical symptoms, pathogen dissemination, etc. (1, 2). The use of these vaccines has greatly enhanced ruminant and public wellbeing around the world, however, in some cases, they have limitations in their ability to induce protective immunity. Thus, rationally designed vaccines along with specific immunization schemes are required to achieve the desired outcome of vaccination against an infectious disease. Vaccine safety is another important consideration, not only in terms of potential risks to the target animal (to which the vaccine is administered), but also to the environment and to consumers of food derived from vaccinated animals (3).Instituto de BiotecnologíaFil: Del Medico Zajac, Maria Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Del Medico Zajac, Maria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Moyano, Roberto Damian. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Colombatti Olivieri, Maria Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Colombatti Olivieri, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Engaging end-user driven recommender systems: personalization through web augmentation

In the past decades recommender systems have become a powerful tool to improve personalization on the Web. Yet, many popular websites lack such functionality, its implementation usually requires certain technical skills, and, above all, its introduction is beyond the scope and control of end-users. To alleviate these problems, this paper presents a novel tool to empower end-users without programming skills, without any involvement of website providers, to embed personalized recommendations of items into arbitrary websites on client-side. For this we have developed a generic meta-model to capture recommender system configuration parameters in general as well as in a web augmentation context. Thereupon, we have implemented a wizard in the form of an easy-to-use browser plug-in, allowing the generation of so-called user scripts, which are executed in the browser to engage collaborative filtering functionality from a provided external rest service. We discuss functionality and limitations of the approach, and in a study with end-users we assess the usability and show its suitability for combining recommender systems with web augmentation techniques, aiming to empower end-users to implement controllable recommender applications for a more personalized browsing experience.Fil: Wischenbart, Martin. Johannes Kepler University Linz; AustriaFil: Firmenich, Sergio Damian. Universidad Nacional de La Plata. Facultad de Informática. Laboratorio de Investigación y Formación en Informática Avanzada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Rossi, Gustavo Héctor. Universidad Nacional de La Plata. Facultad de Informática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Bosetti, Gabriela Alejandra. Universidad Nacional de La Plata. Facultad de Informática; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Kapsammer, Elisabeth. Johannes Kepler University Linz; Austri

Fatores que influenciam no risco de queda das arvores urbanas em Rafaela, Argentina

Understanding the factors that affect tree hazard condition is important to mitigate it. To quantify these factors for Fraxinus americana L., Morus nigra L., and Populus deltoides Marshall trees we have conducted a survey recording their hazard rating according to the TRAQ methodology. The following covariates were registered for each tree: the number of single tree defects in root collar, trunk and scaff old branches, trunk diameter at breast height (DBH), crown class, crown asymmetry, and the size of the tree pit. A multi-category logistic regression model was fi tted to thedata using the backward step-wise procedure. Among the factors considered, only the species, crown class, number of single defects in root collar and scaffold branches significantly aff ected the tree hazard condition. P. deltoids trees had higher values of hazard compared to F. Americana and M. nigra trees. Trees with co-dominant crowns presented a higher probability of being classified as medium and high hazardous than trees with suppressed and dominant crowns. Finally, every single defect observed on root collar and scaffold branches increased the probability of a tree being of medium or high hazardous, by about 3.00 and 1.44 times, respectively. Other factors like crown asymmetry, DBH, and the tree pit size did not have a signifi cant effect on the score of tree hazard. Our results demonstrate that the species selection is an important tool to manage urban tree hazard under the study conditions. At the species level, efforts should be directed towards minimizing defects in root collar, trunk and scaffold branches.A compreensão dos fatores que afetam o risco de queda de árvores inteiras o suas partes (galhos estruturais e caule) é importante para amenizar tais problemas. Para quantifi car esses fatores realizou-se uma análise do risco de Fraxinus americana L., Morus nigra L. e Populus deltoides Marshall por meio da metodologia TRAQ. Além disso, registraram-se as seguintes variáveis: número de defeitos no coleto, tronco e galhos estruturais, diâmetro do caule à altura do peito (DAP), classe de copa, assimetria da copa, espaço de crescimento da árvore e a espécie. Ajustou-se um modelo de regressão logística multi-categoria usando o procedimento backward stepwise. Os fatores que infl uenciaram signifi cativamente o risco de queda das árvores avaliadas foram: espécie, classe de copa, o número de defeitos no coleto e nos galhos estruturais. As árvores de P. deltoides tiveram valores de risco maiores em comparação às árvores de F. americana e M. nigra. As árvores de copas codominantes tiveram maior probabilidade de ser classifi cada como de risco médio ou alto do que as árvores de copas dominantes e intermediarias. Por último, cada defeito observado no coleto aumentou em três vezes a probabilidade das árvores de ser classifi cadas como de risco médio ou alto. No entanto, essas probabilidades aumentaram em 1,44 vezes por cada defeito observado nos galhos estruturais. Porém, outros fatores como DAP, assimetria da copa e o espaço de crescimento não mostraram relação com o grau de risco. Por tanto, os dados demostraram que a seleção de espécies adequadas é a ferramenta mais importante para diminuir o risco de queda das árvores urbanas e que, para cada espécie, os esforços devem ser orientados à minimização dos defeitos no coleto, tronco e galhos principais.Fil: Castro, Damian Cesar. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Alesso, Carlos Agustín. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Iaconis, Ariana. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; ArgentinaFil: Cerino, María Carolina. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Buyatti, Marcela Alejandra. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentin

Changes in the Reproductive Traits of Low-chill Peach Tree in Response to Reproductive Shoot Pruning after Harvesting

ABSTRACT: At the central-east area of the Santa Fe province (Argentina), early maturing peach varieties characterised by a long remaining growing season after harvest are used. The aim of this works was to study the effects of fruit-bearing shoots renovation, performed just after harvest, on different vegetative and reproductive parameters of peach tree, in comparison with the traditional winter pruning. After harvest, a group of plants remained unpruned as control trees (T0), whereas another group was pruned (T1). Pruning consisted of heading back or thinning out the recently harvested bearing shoots. Green pruning significantly increased the relative radiation measured in the canopy for more than 80 days after pruning, modifying the architecture of the aerial part of the tree. The relative proportion of the different bearing shoots was not affected by pruning treatment but green pruning increased the proportion of mixed shoots that arose from the main tree scaffold (+15%) and those that were inserted in the intermediate strata of plant height. Trees pruned after harvest were able to compensate the leaf area removed by the emission of new branches (+108%) in the remained shoots, but were unable to compensate TCA annual evolution (-37.4%) and plant size (?7 to -26%). Green pruning extended the vegetative growth period during autumn, delaying leaf senescence (+10 days) and dormancy induction. Tree yield and fruit size were not affected by pruning treatments.Fil: Weber, M.. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; ArgentinaFil: Castro, Damian Cesar. Universidad Nacional del Litoral; ArgentinaFil: Micheloud, Norma. Universidad Nacional del Litoral; ArgentinaFil: Bouzo, Carlos Alberto. Universidad Nacional del Litoral; ArgentinaFil: Buyatti, Marcela Alejandra. Universidad Nacional del Litoral; ArgentinaFil: Gariglio, N.. Universidad Nacional del Litoral; Argentin

Protection efficacy of Argentinian isolates of Mycobacterium avium subsp. paratuberculosis with different genotypes and virulence in a murine model

Paratuberculosis is a chronic disease caused by Mycobacterium avium subsp. paratuberculosis (Map). The disease causes economic losses and, therefore, it is imperative to follow proper control strategies, which should include an effective vaccine. Several strategies have assessed the virulence and immune response of Map strains that could be used as a vaccine. This study evaluates the degree of virulence, immune response, and protection of Argentinian strains of Map with different genotype in a murine model. Four local isolates (Cattle type) with different genotypes (analyzed by MIRU-VNTR and SSRs) were selected and evaluated in a virulence assay in BALB/c mice. This assay allowed us to differentiate virulent and low-virulence Map strains. The less virulent strains (1543/481 and A162) failed to induce a significant production of the proinflammatory cytokine IFNg, whereas the virulent strain 6611 established infection along with a proinflammatory immune response. On the other hand, the virulent strain 1347/498 was efficient in establishing a persistent infection, but failed to promote an important Th1 response compared with 6611 at the evaluated time. We selected the low-virulence strain 1543/498 as a live vaccine and the virulent strain 6611 as a live and inactivated vaccine in a protection assay in mice. Strain 1543/481 failed to protect the animals from challenge, whereas strain 6611, in its live and inactivated form, significantly reduced the CFUs count in the infected mice, although they had different immunological response profiles. The inactivated virulent strain 6611 is a potential vaccine candidate against paratuberculosis to be tested in cattle.Fil: Colombatti Olivieri, María Alejandra. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Moyano, Roberto Damian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Travería, Gabriel Eduardo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Clínica. Centro de Diagnóstico e Investigaciones Veterinarias; ArgentinaFil: Alvarado Pinedo, María Fiorella. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Clínica. Centro de Diagnóstico e Investigaciones Veterinarias; ArgentinaFil: Mon, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Gravisaco, Maria Jose Federica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Delgado, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; ArgentinaFil: Santangelo, María de la Paz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Class I TCP transcription factors regulate trichome branching and cuticle development in Arabidopsis

Trichomes and the cuticle are two specialized structures of the aerial epidermis that are important for plant organ development and interaction with the environment. In this study, we report that Arabidopsis thaliana plants affected in the function of the class I TEOSINTE BRANCHED 1, CYCLOIDEA, PCF (TCP) transcription factors TCP14 and TCP15 show overbranched trichomes in leaves and stems and increased cuticle permeability. We found that TCP15 regulates the expression of MYB106, a MIXTA-like transcription factor involved in epidermal cell and cuticle development, and overexpression of MYB106 in a tcp14 tcp15 mutant reduces trichome branch number. TCP14 and TCP15 are also required for the expression of the cuticle biosynthesis genes CYP86A4, GPAT6, and CUS2, and of SHN1 and SHN2, two AP2/EREBP transcription factors required for cutin and wax biosynthesis. SHN1 and CUS2 are also targets of TCP15, indicating that class I TCPs influence cuticle formation acting at different levels, through the regulation of MIXTA-like and SHN transcription factors and of cuticle biosynthesis genes. Our study indicates that class I TCPs are coordinators of the regulatory network involved in trichome and cuticle development.Fil: Camoirano, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Arce, Agustín Lucas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Ariel, Federico Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Alem, Antonela Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Gonzalez, Daniel Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Viola, Ivana Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentin

Trajectories and experiences of migration between Paraguay and Argentina: Paraguayan workers in forestry production (Delta of Paraná River)

En este trabajo analizamos las trayectorias y experiencias migratorias de jóvenes paraguayos que trabajan en la producción forestal en el Delta Inferior del río Paraná, desempeñándose en tareas estacionales que son consideradas duras y sacrificadas. Este caso es un ejemplo de la manera en que las migraciones favorecen los procesos de acumulación de capital en el agro en contextos en los que se agudiza la precarización y flexibilización del trabajo rural. Contrastamos las características socio-demográficas de este grupo con las de la mayoría de los paraguayos que, en Argentina, se dirigen hacia áreas urbanas. Planteamos que la expansión de la forestación en el Delta fue favorecida por su participación en un mercado laboral segmentado destinado a migrantes recientes. Señalamos los estereotipos que legitiman su asignación a las peores posiciones de trabajo. Finalmente, analizamos sus trayectorias y experiencias migratorias y laborales.In this article we analyze the trajectories and experiences of migration amongst young male Paraguayan workers. These workers are employed in forestry production in the Lower Delta of Paraná River, performing seasonal tasks that are considered hard and slaughtered. This is an example of the ways labor migrations favor the processes of capital accumulation in agriculture in contexts where precarization of rural labor is exacerbated. We contrasted the socio-demographic characteristics of this group of Paraguayan workers with the characteristics of the vast majority of Paraguayans who, in Argentina, migrate to urban areas. We argue that the expansion of afforestation in Delta was favored by the participation of these young male Paraguayan workers in a segmented labor market aimed at recent migrants. We highlight the stereotypes that legitimize their assignment to the worst positions of this labor market. Finally, we analyze their trajectories and experiences of migration and laborFil: Pizarro, Cynthia Alejandra. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Extensión y Sociología Rurales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; ArgentinaFil: Ortiz, Damian Gabriel. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Extensión y Sociología Rurales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; ArgentinaFil: Straccia, Patricio Hernán. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Extensión y Sociología Rurales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentin

Polvo de ladrillo como sustrato para techos verdes extensivos

Los techos verdes extensivos (TVE) contienen menos de 15 cm de sustrato, el cual debe ser estable, con baja densidad, alta aireación y suficiente retención de agua y nutrientes para el adecuado desarrollo vegetal. En busca de componentes alternativos para formular este tipo de sustratos, el objetivo de este trabajo fue evaluar sustratos con polvo de ladrillo y su efecto sobre el crecimiento de especies del género Sedum L. El ensayo fue conformado por cuatro tratamiento/sustratos compuestos por piedra pómez (P), microleca (M), perlita (Pe), turba de Sphagnum (T), zeolita enriquecida (Z) y polvo de ladrillo (PL) en volúmenes indicados por los subíndices: 1) P20:M25:Pe40:Z15; 2) PL85:Z15; 3) P20:M25:Pe30:Z15:T10; y 4) PL75:Z15:T10. Se confeccionaron tres mesadas divididas en cuatro unidades experimentales de 1,2 m2. Las especies del género Sedum evaluadas fueron S. acre, S. alba, S. mexicanum y S. ruflexum. Se realizó análisis físicos y químicos a todos los sustratos. A cada planta se midió la superficie (cm2) cubierta por la parte vegetativa y la supervivencia. Todos los sustratos tuvieron un valor de pH superior a 6,3 y un valor inicial de conductividad eléctrica alto, pero menor al final del ensayo. La porosidad y aireación en todos los sustratos fue mayor que la capacidad de retención de agua. La superficie cubierta por S. acre fue mayor en el sustrato PL75:Z15T10, y por S. alba en los sustratos P20:M25:Pe40:Z15 y PL75:Z15T10. Sedum mexicanum cubrió menor superficie en el sustrato PL85:Z15, y con S. reflexum no hubo diferencias significativas (P : 0,6114). El polvo de ladrillo se muestra como una alternativa viable para su uso en TVE. Palabras clave: Techo verde, sustrato, polvo ladrillo, Sedum, sustentable, residuo de construcción, medio de cultivo.Extensive green roofs (EGR) contain less than 15 cm of substrate, which must be stable, with low density, high aeration and sufficient water and nutrient retention for adequate plant development. In search of alternative components to formulate this type of substrates, the objective of this work was to evaluate substrates with brick dust and its effect on the growth of species of the genus Sedum L. The trial consisted of four treatments/substrates composed of pumice stone (P), microleca (M), perlite (Pe), Sphagnum peat (T), enriched zeolite (Z) and brick dust (PL) in volumes indicated by the subscripts: 1) P20:M25:Pe40:Z15; 2) PL85:Z15; 3) P20:M25:Pe30:Z15:T10; and 4) PL75:Z15:T10. Three tables were made and divided into four experimental units of 1.2 m2. The Sedum species evaluated were S. acre, S. alba, S. mexicanum and S. ruflexum. Physical and chemical analyzes were carried out on all substrates. The surface area (cm2) covered by the vegetative part and survival was measured for each plant. All substrates had a pH value greater than 6.3 and a high initial electrical conductivity value, but low at the end of the trial. The aeration porosity in all substrates was greater than the water retention capacity. The surface covered by S. acre was greater in the substrate PL75: Z15T10 and by S. alba in the substrates P20: M25: Pe40: Z15 and PL75: Z15T10. Sedum mexicanum covered less surface in the substrate PL85:Z15, and no differences were observed with S. reflexum (P: 0.6114). Brick dust is a viable alternative for use in EGR.EEA Cerro AzulFil: Barbaro, Lorena Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cerro Azul; ArgentinaFil: Sisaro, Damian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; ArgentinaFil: Stancanelli, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; ArgentinaFil: Soto, Maria Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentin

Upgrading the nutritional value of rice bran by Solid-State fermentation with pleurotus sapidus

Solid-state fermentation (SSF) of rice bran (RB) employing the edible fungus Pleurotus sapidus was investigated as a process strategy to improve the nutritional quality of this low-cost and abundant substrate. During fermentation, samples were withdrawn at different time intervals (4, 6, and 10 days) and further analyzed. Established methods were deployed to monitor the changes in nutritional composition (carbohydrates, proteins, ash, and lipids). Additionally, changes in fatty acid composition was studied as a function of culture progress. Results showed that the SSF of rice bran increased total carbohydrates from 36.6% to 50.2%, total proteins from 7.4% to 12.8%, and ash from 7.6% to 11.5%. However, the total lipid content was reduced from 48.5% to 27.8%. The fatty acid (FA) composition of RB included mainly oleic, linoleic, and palmitic acids. Upon fermentation with P. sapidus, small differences were found: linoleic acid and oleic acid content were increased by 0.4% and 1.1%, respectively, while palmitic acid content was reduced by 0.8%. This study demonstrated an improvement in the nutritional quality of RB after fermentation with P. sapidus, since protein, carbohydrates, minerals, and specific FA components were increased. As a whole, our results indicate that fermented rice bran could be used as a high-quality animal feed supplement.Fil: Omarini, Alejandra Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina. Universitat Bremen. School of Enigineerring and Science Jacobs; AlemaniaFil: Labuckas, Diana Ondina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Zunino, María Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Pizzolitto, Romina Paola. Universidad Nacional de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Fernández Lahore, Marcelo. Universitat Bremen. School of Enigineerring and Science Jacobs; AlemaniaFil: Barrionuevo, Luis Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Zygadlo, Julio Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentin

Assessment of tuberculosis biomarkers in paratuberculosis-infected cattle

Introduction: Mycobacterium bovis and Mycobacterium avium subsp. paratuberculosis, respectively the causative agents of bovine tuberculosis (bTB) and bovine paratuberculosis (PTB), share a high number of antigenic proteins. This characteristics makes the differential diagnosis of the diseases difficult. The interferon gamma (IFN-γ), C-X-C motif chemokine ligand 10 (CXCL10), matrix metallopeptidase 9 (MMP9), interleukin 22 (IL-22) and thrombospondin 1 (THBS1) bovine genes have already been shown to be accurate transcriptional biomarkers of bTB. In order to improve the diagnosis of bTB and PTB, in the present study we evaluated the risk of false positivity of these bTB biomarkers in cattle with PTB. Material and methods: The transcription of these genes was studied in 13 PTB-infected cattle, using Mycobacterium avium subsp. paratuberculosis (MAP)-stimulated peripheral blood mononuclear cells (PBMC). Results: Overall, the levels of IFN-γ, CXCL10, MMP9 and IL-22 transcripts in MAP-stimulated PBMC failed to differentiate animals with PTB from healthy animals. However, as bTB-afflicted cattle do, the MAP-infected group also displayed a lower level of THBS1 transcription than the non-infected animals. Conclusion: The results of this study add new specificity attributes to the levels of transcription of IFN-γ, CXCL10, MMP9 and IL-22 as biomarkers for bTB.Instituto de BiotecnologíaFil: Klepp, Laura Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Klepp, Laura Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Colombatti Olivieri, Maria Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Colombatti Olivieri, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Moyano, Roberto Damian. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Romano, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Malovrh, Tadej. University of Ljubljana. Veterinary Faculty. Institute for Microbiology and Parasitology; EsloveniaFil: Ocepek, Matjaž. University of Ljubljana. Veterinary Faculty. Institute for Microbiology and Parasitology; EsloveniaFil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Blanco, Federico Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Bigi, Fabiana. Consejo Nacional de investigaciones Científicas y Tecnológicas; Argentin