Some Observations on Transitory Stall in Conical Diffusers

Results from an experimental investigation on the flow through conical diffusers are presented. The mean and fluctuating velocity fields are compared for three diffusers with total diffusion angles of 16, 20 and 24 degrees, in the throat Mach number (M sub t) range of 0.05 to 0.95. Each of the diffusers were 14 cm long and had a 5.08 cm inlet diameter, and the flow exited into the ambient. The boundary layer at the throat was thin with the throat diameter (D sub t) to momentum thickness (O) ratio being as high as 800 at M(sub t) = 0.4. While the 16 deg diffuser flow exited with a top-hat mean velocity profile, increasing losses due to increasing separation resulted in fuller profiles for the 20 and 24 degree cases. A detailed flow field study was conducted for the 16 deg. diffuser. The u'-spectrum, measured at the exit plane, exhibited a peak apparently due to the ensuing jet column instability throughout the M(sub t) range covered. In addition, a much lower frequency spectral peak also occurred in the M(sub t) range of 0.3 to 0.7. Both of the spectral peaks were due to axisymmetric flow fluctuations. A self-sustaining flow oscillation occurred in the M(sub t) range of 0.6 to 0.85, emitting a loud tone, when the jet column instability frequency matched the resonance frequency of the diffuser. Limited data showed that artificial acoustic excitation was effective in reducing the flow fluctuations, with a resultant increase in the pressure recovery, at low M(sub t)

Priorities for sustainable turfgrass management: a research and industry perspective

This paper provides a brief review and assessment of the key environmental, regulatory and technical issues facing the turfgrass sector with specific reference to the European context. It considers the range of externalities or ‘drivers for change' facing the industry, and the challenges and opportunities available for promoting and achieving more sustainable turfgrass management within the sports, landscape and amenity sectors. The analysis confirms that there are a number of key areas where a concerted research and industrial effort is required. These include responding to the pressures from government demands for greater environmental regulation, the increasing pressure on natural resources (notably water, energy and land), the emerging role of turf management in supporting ecosystem services and enhancing biodiversity, the continued need to promote integrated pest management, and the looming challenges posed by a changing climate, and urgent need to adapt. Whilst many of these externalities appear to be risks to the sports turf industry, there will also be significant opportunities, for those where the labour, energy and agronomic costs are minimized and where the drive to adopt a multifunctional approach to sportsturf management is embraced

Hydrogen atom in phase space. The Kirkwood-Rihaczek representation

We present a phase-space representation of the hydrogen atom using the Kirkwood-Rikaczek distribution function. This distribution allows us to obtain analytical results, which is quite unique because an exact analytical form of the Wigner functions corresponding to the atom states is not known. We show how the Kirkwood-Rihaczek distribution reflects properties of the hydrogen atom wave functions in position and momentum representations.Comment: 5 pages (and 5 figures

Network Inference via the Time-Varying Graphical Lasso

Many important problems can be modeled as a system of interconnected entities, where each entity is recording time-dependent observations or measurements. In order to spot trends, detect anomalies, and interpret the temporal dynamics of such data, it is essential to understand the relationships between the different entities and how these relationships evolve over time. In this paper, we introduce the time-varying graphical lasso (TVGL), a method of inferring time-varying networks from raw time series data. We cast the problem in terms of estimating a sparse time-varying inverse covariance matrix, which reveals a dynamic network of interdependencies between the entities. Since dynamic network inference is a computationally expensive task, we derive a scalable message-passing algorithm based on the Alternating Direction Method of Multipliers (ADMM) to solve this problem in an efficient way. We also discuss several extensions, including a streaming algorithm to update the model and incorporate new observations in real time. Finally, we evaluate our TVGL algorithm on both real and synthetic datasets, obtaining interpretable results and outperforming state-of-the-art baselines in terms of both accuracy and scalability

Data Quality of Surgery for Carotid Artery Stenosis. Are the National Vascular Registries Reliable?

AbstractObjectivesTo study completeness of reporting carotid endarterectomies, including peri-operative stroke and mortality rate, in a national vascular registry, NorKar, and a national administrative registry, The Norwegian Patient Register (NPR).DesignComparative registry-based national study.MaterialsMember hospitals of NorKar, including 89% of carotid endarterectomies in Norway, were compared with relevant data in NPR for the years 2000–2002.MethodsWe compared procedure-codes, diagnosis-codes, in-hospital death and the occurrence of peri-operative stroke after treatment for carotid artery stenosis in the two registries to evaluate completeness.ResultsCompared with the NPR numbers, 16% of carotid endarterectomies were missing in the reports from member hospitals of NorKar. Further, during this three-year period, there was an under-reporting of seven strokes and two deaths. The discrepancy was most pronounced in 2001.ConclusionsThere is an under-reporting of patients operated on for carotid artery stenosis in NorKar according to NPR numbers as well as an under-reporting of early deaths and strokes. There is a need for better quality data in the NorKar Registry. Registry quality would be likely to improve if patient identifiable data were available in both registries

Bacillus subtilis GlcK activity requires cysteines within a motif that discriminates microbial glucokinases into two lineages

BACKGROUND: Bacillus subtilis glucokinase (GlcK) (GenBank NP_390365) is an ATP-dependent kinase that phosphorylates glucose to glucose 6-phosphate. The GlcK protein has very low sequence identity (13.7%) to the Escherichia coli glucokinase (Glk) (GenBank P46880) and some other glucokinases (EC 2.7.1.2), yet glucose is merely its substrate. Our lab has previously isolated and characterized the glcK gene. RESULTS: Microbial glucokinases can be grouped into two different lineages. One of the lineages contains three conserved cysteine (C) residues in a CXCGX(2)GCXE motif. This motif is also present in the B. subtilis GlcK. The GlcK protein occurs in both monomer and homodimer. Each GlcK monomer has six cysteines. All cysteine residues have been mutated, one-by-one, into alanine (A). The in vivo GlcK enzymatic activity was assayed by functional complementation in E. coli UE26 (ptsG ptsM glk). Mutation of the three motif-specific residues led to an inactive enzyme. The other mutated forms retained, or in one case (GlcK(C321A)) even gained, activity. The fluorescence spectra of the GlcK(C321A )showed a red shift and enhanced fluorescence intensity compare to the wild type's. CONCLUSIONS: Our results emphasize the necessity of cysteines within the CXCGX(2)GCXE motif for GlcK activity. On the other hand, the C321A mutation led to higher GlcK(C321A )enzymatic activity with respect to the wild type's, suggesting more adequate glucose phosphorylation

Expression of a novel gene, gluP, is essential for normal Bacillus subtilis cell division and contributes to glucose export

BACKGROUND: The Bacillus subtilis glucokinase operon was predicted to be comprised of the genes, yqgP (now named gluP), yqgQ, and glcK. We have previously established a role for glcK in glucose metabolism. In the absence of enzymes that phosphorylate glucose, such as GlcK and/or enzyme II(Glc), accumulated cytoplasmic glucose can be transported out of the cell. Genes within the glucokinase operon were not previously known to play a role in glucose transport. Here we describe the expression of gluP and its function in glucose transport. RESULTS: We found that transcription of the glucokinase operon was regulated, putatively, by two promoters: σ(A )and σ(H). Putative σ(A )and σ(H)-recognition sites were located upstream of and within gluP, respectively. Transcriptional glucokinase operon – lacZ fusions and Northern blotting were used to analyze the expression of gluP. GluP was predicted to be an integral membrane protein. Moreover, the prediction of GluP structure revealed interesting signatures: a rhomboid domain and two tetracopeptide repeat (TPR) motifs. Microscopic analysis showed that GluP minus cells were unable to divide completely, resulting in a filamentous phenotype. The cells were grown in either rich or minimal medium. We found GluP may be involved in glucose transport. [(14)C]-glucose uptake by the GluP minus strain was slightly less than in the wild type. On the other hand, trehalose-derived glucose in the growth medium of the GluP minus strain was detected in very low amounts. Experimental controls comprised of single or multiple genes mutations within the glucose transporting phosphotransferase system. CONCLUSIONS: gluP seems to be regulated only by a putative σ(A)-dependent promoter. The glucose uptake and export assays suggest that GluP is important for glucose export and may act as an exporter. This also supports the role of the glucokinase operon in glucose utilization

Segregation, precipitation, and \alpha-\alpha' phase separation in Fe-Cr alloys: a multi-scale modelling approach

Segregation, precipitation, and phase separation in Fe-Cr systems is investigated. Monte Carlo simulations using semiempirical interatomic potential, first-principles total energy calculations, and experimental spectroscopy are used. In order to obtain a general picture of the relation of the atomic interactions and properties of Fe-Cr alloys in bulk, surface, and interface regions several complementary methods has to be used. Using Exact Muffin-Tin Orbitals method the effective chemical potential as a function of Cr content (0-15 at.% Cr) is calculated for a surface, second atomic layer and bulk. At ~10 at.% Cr in the alloy the reversal of the driving force of a Cr atom to occupy either bulk or surface sites is obtained. The Cr containing surfaces are expected when the Cr content exceeds ~10 at.%. The second atomic layer forms about 0.3 eV barrier for the migration of Cr atoms between bulk and surface atomic layer. To get information on Fe-Cr in larger scales we use semiempirical methods. Using combined Monte Carlo molecular dynamics simulations, based on semiempirical potential, the precipitation of Cr into isolated pockets in bulk Fe-Cr and the upper limit of the solubility of Cr into Fe layers in Fe/Cr layer system is studied. The theoretical predictions are tested using spectroscopic measurements. Hard X-ray photoelectron spectroscopy and Auger electron spectroscopy investigations were carried out to explore Cr segregation and precipitation in Fe/Cr double layer and Fe_0.95Cr_0.05 and Fe_0.85Cr_0.15 alloys. Initial oxidation of Fe-Cr was investigated experimentally at 10^-8 Torr pressure of the spectrometers showing intense Cr_2O_3 signal. Cr segregation and the formation of Cr rich precipitates were traced by analysing the experimental spectral intensities with respect to annealing time, Cr content, and kinetic energy of the exited electron.Comment: 16 pages, 14 figures, 52 reference

Suspension platform interferometer for the AEI 10\,m prototype: concept, design and optical layout

At present a 10\,m prototype interferometer facility is being set up at the AEI Hannover. One unique feature of the prototype will be the suspension platform interferometer (SPI). The purpose of the SPI is to monitor and stabilise the relative motion between three seismically isolated optical tables. The in-vacuum tables are suspended in an L-shaped configuration with an arm length of 11.65\,m. The design goal of the SPI is to stabilise longitudinal differential displacements to a level of 100\,pm/$\sqrt{\mathrm{Hz}}$ between 10\,mHz and 100\,Hz and relative angular noise of 10\,nrad/$\sqrt{\mathrm{Hz}}$ in the same frequency band. This paper covers the design aspects of the SPI, e.g. cross-coupling between the different degrees of freedom and fibre pointing noise are investigated. A simulation is presented which shows that with the chosen optical design of the SPI all degrees of table motion can be sensed in a fully decoupled way. Furthermore, a proof of principle test of the SPI sensing scheme is shown.Comment: 12 pages, 6 figure

Electronic spectroscopy of FUV-irradiated diamondoids: A combined experimental and theoretical study

Irradiation with high energy photons (10.2 - 11.8 eV) was applied to small diamondoids isolated in solid rare gas matrices at low temperature. The photoproducts were traced via UV absorption spectroscopy. We found that upon ionization the smallest of these species lose a peripheral H atom to form a stable closed-shell cation. This process is also likely to occur under astrophysical conditions for gas phase diamondoids and it opens the possibility to detect diamond-like molecules using their rotational spectrum since the dehydrogenated cations possess strong permanent dipole moments. The lowest-energy electronic features of these species in the UV were found to be rather broad, shifting to longer wavelengths with increasing molecular size. Calculations using time-dependent density functional theory support our experimental findings and extend the absorption curves further into the vacuum ultraviolet. The complete sigma - sigma* spectrum displays surprisingly strong similarities to meteoritic nanodiamonds containing 50 times more C atoms.Comment: 11 pages, 9 figures; published in The Astrophysical Journal; corrected minor mistakes compared to the published pape