P53 tumour-suppressor gene mutations are mainly localised on exon 7 in human primary and metastatic prostate cancer.

Mutations in the p53 tumour-suppressor gene are among the most common genetic alterations in human cancers. In the present study we analysed the mutations in the p53 tumor-suppressor gene in 25 primary and 20 metastatic human prostate cancer specimens. DNA extracted from the paraffin-embedded sections was amplified by hot-start polymerase chain reaction, and p53 gene mutations in the conserved mid-region (exons 4-9) were examined using single-strand conformation polymorphism (SSCP) analysis and immunohistochemistry. In the present study, we used a novel hot-start PCR-SSCP technique using DNA Taq polymerase antibody, which eliminates primer-dimers and non-specific products. Because of this new technique, the results of PCR-SSCP showed very high resolution. Polymerase chain reaction products were sequenced directly for point mutations for the p53 gene. Mutations were found in 2 out of 25 primary prostate cancers (8%) and 4 out of 20 metastatic cancers (20%). Mutations were observed exclusively in exon 7 and not in exons 4, 5, 6, 8 or 9. Nuclear accumulation of p53 protein, determined by immunohistochemistry, correlated with the degree of metastasis in prostatic cancer

Extracting the Omega- electric quadrupole moment from lattice QCD data

The Omega- has an extremely long lifetime, and is the most stable of the baryons with spin 3/2. Therefore the Omega- magnetic moment is very accurately known. Nevertheless, its electric quadrupole moment was never measured, although estimates exist in different formalisms. In principle, lattice QCD simulations provide at present the most appropriate way to estimate the Omega- form factors, as function of the square of the transferred four-momentum, Q2, since it describes baryon systems at the physical mass for the strange quark. However, lattice QCD form factors, and in particular GE2, are determined at finite Q2 only, and the extraction of the electric quadrupole moment, Q_Omega= GE2(0) e/(2 M_Omega), involves an extrapolation of the numerical lattice results. In this work we reproduce the lattice QCD data with a covariant spectator quark model for Omega- which includes a mixture of S and two D states for the relative quark-diquark motion. Once the model is calibrated, it is used to determine Q_Omega. Our prediction is Q_Omega= (0.96 +/- 0.02)*10^(-2) efm2 [GE2(0)=0.680 +/- 0.012].Comment: To appear in Phys. Rev. D. Version with small modifications. 8 pages, 1 figur

Firm Opacity Lies in the Eye of the Beholder

We classify and test empirical measures of firm opacity and document theoretical and empirical inconsistencies across these proxies by testing the relative opacity of banks versus non-banks. We evaluate the effectiveness of these proxies by observing the effect of two cleanly identified shocks to firm-specific information: credit rating initiation and inclusion in the S&P 500 index. Using a difference-in-difference approach, we compare firms that are newly rated and firms that are included in the S&P 500 index with a propensity matched sample of “unchanged” firms. We find that only the number of analysts and Amihud's illiquidity ratio provide consistent patterns across different estimation specifications and different econometric settings. These two proxies show that banks are more opaque than non-banks. Based on our tests, we recommend that these proxies be used as the primary measures of firm opacity

Semi-leptonic Octet Baryon Weak Axial-Vector Form Factors in the Chiral Constitutent Quark Model

The weak vector and axial-vector form factors have been investigated for the semi-leptonic octet baryon decays in the chiral constituent quark model with configuration mixing (\chiCQM_{config}). The effects of SU(3) symmetry breaking and configuration mixing have also been investigated and the results are not only in good agreement with existing experimental data but also show improvement over other phenomenological models.Comment: 5 pages, 2 tables. Presented at the 18th International Spin Physics Symposium, University of Virginia, USA, October 6-11, 200

Chiral QCD, General QCD Parameterization and Constituent Quark Models

Several recent papers -using effective QCD chiral Lagrangians- reproduced results obtained with the general QCD parameterization (GP). These include the baryon 8+10 mass formula, the octet magnetic moments and the coincidental nature of the "perfect" -3/2 ratio between the magnetic moments of p and n. Although we anticipated that the GP covers the case of chiral treatments, the above results explicitly exemplify this fact. Also we show by the GP that -in any model or theory (chiral or non chiral) reproducing the results of exact QCD- the Franklin (Coleman Glashow) sum rule for the octet magnetic moments must be violated.Comment: 10 pages, Latex; abridged version (same results), removed some reference

Soft eSkin:distributed touch sensing with harmonized energy and computing

Inspired by biology, significant advances have been made in the field of electronic skin (eSkin) or tactile skin. Many of these advances have come through mimicking the morphology of human skin and by distributing few touch sensors in an area. However, the complexity of human skin goes beyond mimicking few morphological features or using few sensors. For example, embedded computing (e.g. processing of tactile data at the point of contact) is centric to the human skin as some neuroscience studies show. Likewise, distributed cell or molecular energy is a key feature of human skin. The eSkin with such features, along with distributed and embedded sensors/electronics on soft substrates, is an interesting topic to explore. These features also make eSkin significantly different from conventional computing. For example, unlike conventional centralized computing enabled by miniaturized chips, the eSkin could be seen as a flexible and wearable large area computer with distributed sensors and harmonized energy. This paper discusses these advanced features in eSkin, particularly the distributed sensing harmoniously integrated with energy harvesters, storage devices and distributed computing to read and locally process the tactile sensory data. Rapid advances in neuromorphic hardware, flexible energy generation, energy-conscious electronics, flexible and printed electronics are also discussed. This article is part of the theme issue ‘Harmonizing energy-autonomous computing and intelligence’

MicroRNA-383 located in frequently deleted chromosomal locus 8p22 regulates CD44 in prostate cancer.

A major genomic alteration in prostate cancer (PCa) is frequent loss of chromosome (chr) 8p with a common region of loss of heterozygosity (LOH) at chr8p22 locus. Genomic studies implicate this locus in the initiation of clinically significant PCa and with progression to metastatic disease. However, the genes within this region have not been fully characterized to date. Here we demonstrate for the first time that a microRNA component of this region-miR-383-is frequently downregulated in prostate cancer, has a critical role in determining tumor-initiating potential and is involved in prostate cancer metastasis via direct regulation of CD44, a ubiquitous marker of PCa tumor-initiating cells (TICs)/stem cells. Expression analyses of miR-383 in PCa clinical tissues established that low miR-383 expression is associated with poor prognosis. Functional data suggest that miR-383 regulates PCa tumor-initiating/stem-like cells via CD44 regulation. Ectopic expression of miR-383 inhibited tumor-initiating capacity of CD44+ PCa cells. Also, 'anti-metastatic' effects of ectopic miR-383 expression were observed in a PCa experimental metastasis model. In view of our results, we propose that frequent loss of miR-383 at chr8p22 region leads to tumor initiation and prostate cancer metastasis. Thus, we have identified a novel finding that associates a long observed genomic alteration to PCa stemness and metastasis. Our data suggest that restoration of miR-383 expression may be an effective therapeutic modality against PCa. Importantly, we identified miR-383 as a novel PCa tissue diagnostic biomarker with a potential that outperforms that of serum PSA

Strangeness and Chiral Symmetry Breaking

The implications of chiral symmetry breaking and SU(3) symmetry breaking have been studied in the chiral constituent quark model ($\chi$CQM). The role of hidden strangeness component has been investigated for the scalar matrix elements of the nucleon with an emphasis on the meson-nucleon sigma terms. The $\chi$CQM is able to give a qualitative and quantitative description of the "quark sea" generation through chiral symmetry breaking. The significant contribution of the strangeness is consistent with the recent available experimental observations.Comment: 10 pages, 1 table. To appear in Mod. Phys. Lett.