Transcription of brain natriuretic peptide and atrial natriuretic peptide genes in human tissues

We have compared the expression of atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) genes in various human tissues using a quantitative polymerase chain reaction technique. Tissues of three human subjects, obtained at autopsy, were analyzed. BNP transcripts could be detected in the central nervous system, lung, thyroid, adrenal, kidney, spleen, small intestine, ovary, uterus, and striated muscle. ANP transcripts could also be demonstrated in various human extracardiac tissues including several endocrine organs. In all peripheral tissues, the level of both natriuretic peptide transcripts was approximately 1-2 orders of magnitude lower than in cardiac ventricular tissues. This distribution is in marked contrast to the much lower level of ANP and BNP transcripts present in extracardiac rat tissues (generally less than 1/1000 of ventricles). These data suggest differential expression of the two natriuretic peptide genes in cardiac and extracardiac tissues in man. Furthermore, the presence of local synthesis of ANP and BNP in various peripheral organs suggests paracrine and/or autocrine function of these natriuretic peptides

The competitive productivity (CP) of tourism destinations: an integrative conceptual framework and a reflection on big data and analytics

Purpose: The purpose of this study is twofold. First, this study elaborates an integrative conceptual framework of tourism destination competitive productivity (TDCP) by blending established destination competitiveness frameworks, the competitive productivity (CP) framework and studies pertaining to big data and big data analytics (BDA) within destination management information systems and smart tourism destinations. Second, this study examines the drivers of TDCP in the context of the ongoing 4th industrial revolution by conceptualizing the destination business intelligence unit (DBIU) as a platform able to create sustained destination business intelligence under the guise of BDA, useful to support destination managers to achieve the tourism destination’s economic objectives. Design/methodology/approach: In this work, the authors leverage both extant literature (under the guise of research on CP, tourism destination competitiveness [TDC] and destination management information systems) and empirical work (in the form of interviews and field work involving destination managers and chief executive officers of destination management organizations and convention bureaus, as well as secondary data) to elaborate, develop and present an integrative conceptual framework of TDCP. Findings: The integrative conceptual framework of TDCP elaborated has been found helpful by a number of destination managers trying to understand how to effectively and efficiently manage and market a tourism destination in today’s fast-paced, digital and hypercompetitive environment. While DBIUs are at different stages of implementation, often as part of broader smart destination initiatives, it appears that they are increasingly fulfilling the purpose of creating sustained destination business intelligence by means of BDA to help tourism destinations achieve their economic goals. Research limitations/implications: This work bears several practical implications for tourism policymakers, destination managers and marketers, technology developers, as well as tourism and hospitality firms and practitioners. Tourism policymakers could embed TDCP into tourism and economic policies, and destination managers and marketers might build and make use of platforms such as the proposed DBIU. Technology developers need to understand that designing destination management information systems in general and more specifically DBIUs requires an in-depth analysis of the stakeholders that are going to contribute, share, control and use BDA. Originality/value: To the best of the authors’ knowledge, this study constitutes the first attempt to integrate the CP, TDC and destination management information systems research streams to elaborate an integrative conceptual framework of TDCP. Second, the authors contribute to the Industry 4.0 research stream by examining the drivers of tourism destination CP in the context of the ongoing 4th industrial revolution. Third, the authors contribute to the destination management information systems research stream by introducing and conceptualizing the DBIU and the related sustained destination business intelligence

Integrin-linked kinase is required for TGF-_1 induction of dermal myofibroblast differentiation

Cutaneous repair after injury requires activation of resident dermal fibroblasts and their transition to myofibroblasts. The key stimuli for myofibroblast formation are activation of transforming growth factor-b (TGF-b) receptors and mechanotransduction mediated by integrins and associated proteins. We investigated the role of integrin-linked kinase (ILK) in TGF-b1 induction of dermal fibroblast transition to myofibroblasts. ILK-deficient fibroblasts treated with TGF-b1 exhibited attenuation of Smad 2 and 3 phosphorylation, accompanied by impaired transcriptional activation of Smad targets, such as a-smooth muscle actin. These alterations were not limited to Smad-associated TGF-b1 responses, as stimulation of noncanonical mitogenactivated protein kinase pathways by this growth factor was also diminished in the absence of ILK. ILK-deficient fibroblasts exhibited abnormalities in the actin cytoskeleton, and did not form supermature focal adhesions or contractile F-actin stress fibers, indicating a severe impairment in their capacity to differentiate into myofibroblasts. These defects extended to the inability of cells to contract extracellular matrices when embedded in collagen lattices. We conclude that ILK is necessary to transduce signals implicated in the transition of dermal fibroblasts to myofibroblasts originating from matrix substrates and TGF-b1

Symmetry breaking in small rotating cloud of trapped ultracold Bose atoms

We study the signatures of rotational and phase symmetry breaking in small rotating clouds of trapped ultracold Bose atoms by looking at rigorously defined condensate wave function. Rotational symmetry breaking occurs in narrow frequency windows, where the ground state of the system has degenerated with respect to the total angular momentum, and it leads to a complex wave function that exhibits vortices clearly seen as holes in the density, as well as characteristic vorticity. Phase symmetry (or gauge symmetry) breaking, on the other hand, is clearly manifested in the interference of two independent rotating clouds.Comment: 4 pages, 2 figure

Management of an invasive plant in a Mediterranean Protected Area: the experience of Senecio deltoideus in Italy

Biological invasions are one of the most serious threats to global biodiversity and ecosystem integrity. The problem is growing year by year and a large number of protected areas worldwide are today invaded by at least one exotic species. In this study, we tested the eco-friendly and cost-effective weeding control of Senecio deltoideus in a Regional Protected Area in the North Mediterranean region. During a two years experiment, four techniques compatible with the local laws on protected areas (natural-herbicide, flame-weeding, mulching and mowing) were applied five times a season on sixty plots, compared with fifteen untreated controls. All techniques were effective in reducing Senecio covering: after the first year the maximum covering was limited to 37.93% (flame weeding) with a mean value of 10%; after second year the covering was further reduced (17.02% max; 2% mean). Interestingly, during the second year all plots submitted to a one-year treatment showed an enduring control of S. deltoideus covering (40.73% max; 20% mean). Taking into account feasibility and their impact on the environment, the weeding control recommendable for S. deltoideus is mowing. In a long-time management strategy, the selected treatment could be applied every two years with a drastic reduction in costs for the manager of the protected area

Ordered structures in rotating ultracold Bose gases

The characterization of small samples of cold bosonic atoms in rotating microtraps has recently attracted increasing interest due to the possibility to deal with a few number of particles per site in optical lattices. We analyze the evolution of ground state structures as the rotational frequency $\Omega$ increases. Various kinds of ordered structures are observed. For $N<10$ atoms, the standard scenario, valid for large sytems, is absent, and only gradually recovered as $N$ increases. The vortex contribution to the total angular momentum $L$ as a function of $\Omega$ ceases to be an increasing function of $\Omega$, as observed in experiments of Chevy {\it et al.} (Phys. Rev. Lett. 85, 2223 (2000)). Instead, for small $N$, it exhibits a sequence of peaks showing wide minima at the values of $\Omega$, where no vortices appear.Comment: 35 pages, 17 figure