Results of the implementation of turnaround strategies for the Maquoketa United Methodist Church based on natural church development

https://place.asburyseminary.edu/ecommonsatsdissertations/1306/thumbnail.jp

MAGNETIC FIELD DESIGN TO REDUCE SYSTEMATIC EFFECTS IN NEUTRON ELECTRIC DIPOLE MOMENT MEASUREMENTS

Charge-Conjugation (C) and Charge-Conjugation-Parity (CP) Violation is one of the three Sakharov conditions to explain via baryogenesis the observed baryon asymmetry of the universe (BAU). The Standard Model of particle physics (SM) contains sources of CP violation, but cannot explain the BAU. This motivates searches for new physics beyond the standard model (BSM) which address the Sakharov criteria, including high-precision searches for new sources of CPV in systems for which the SM contribution is small, but larger effects may be present in BSM theories. A promising example is the search for the electric dipole moment of the neutron (nEDM), which is a novel system to observe CPV due to the initial and final state being identical. A non-zero measurement necessarily requires violation of P and T discrete symmetries; invoking CPT invariance requires that CP is violated. There are BSM theories which predict a magnitude for the nEDM larger than SM predictions, so that such studies are beneficial at setting constraints on new physics. The current experimental limit of dn \u3c 3.0 x 10-26 e cm at 90% CL as set by the Institut Laue-Langevin (ILL) [1] was largely limited by systematic effects related to the magnetic field. The research presented here supported technical progress toward a new measurement of the nEDM, with the goal of improving the result by an order of magnitude. A novel approach to the problem of limiting systematics is proposed, studied in Monte Carlo simulations, and an optimized prototype was constructed for use in a magnetic resonance experiment

Purification and Crystallization Trials of the Dihydroorotase from Methanococcus jannaschii

Dihydroorotase is the enzyme that catalyzes the third step of the de novo biosynthesis of pyrimidines. M. jannaschii is a hyperthermophillic archaeon that can serve as a model organism for research purposes. This experiment is a first step toward elucidating the structure of the dihydroorotase in M. jannaschii. The enzyme was purified by salting out and heating the solution and then putting the supernatant through cation exchange chromatography and hydrophobic interaction chromatography. Twenty-four conditions were tested to determine if a crystal of dihydroorotase could be formed. Two of these conditions led to preliminary crystal formation. These findings can be utilized to determine which other conditions to test to form a crystal. Once a crystal is formed, it can undergo X-ray crystallography to determine its structure. The structure of dihydroorotase in M. jannaschii can elucidate what structural characteristics allow this archaeon to survive in extreme heat and provides further understanding of the diversity within the dihydroorotase family of proteins. It can also give further insight into this pathway in humans.https://engagedscholarship.csuohio.edu/u_poster_2017/1030/thumbnail.jp