Effect of different levels of n-3 HUFA on larvae culture performances of Beluga (Huso huso) fish

The effects of n-3 highly unsaturated fatty acids (n-3 HUFA) were studied on Beluga (Huso huso) fish larvae by feeding fish larvae with live food enriched with 4 different oils (ICES 30/4, tuna eye oil, flaxseed oil and linseed oil) containing different levels of n-3 HUFA including 27.19, 20.33, 12.71 and 0.39 mg g^-1 dry weight of oil, respectively. Fish larvae weighing 30.00±2.00 mg were randomly divided into 12 groups of 150 fish each and triplicates fed the 4 experimental diets for 3 weeks. There were no differences in survival rates, but significant differences were found in growth rates and fatty acid composition of the fish larvae after 30 days. Fish fed low levels of dietary n-3 HUFA (0.39 mg g^-1 DW) showed poor n-3 HUFA contents but these fatty acids improved with the elevation of the n-3 HUFA levels in the diet up to 20 mg g^-1 DW. Fish fed the flaxseed oil showed the highest level of crude lipid 15.00±3.01). Results showed that tuna eye oil and ICES30/4-enriched live food (4.83 ±1.29 and 4.61 ±0.99 mg g^-1 DW, respectively) showed the highest n-3 HUFA content for sturgeon fish larvae

Study of barriers and strategies for development of ornamental fish industry in Iran: “case study of Markazi Province”

Caspian Sea, Persian Gulf and the natural aquatic environment, made Iran’s geo-strategic and socioeconomic situation of the fisheries and aquaculture sector unique. This sector in the three areas of “ecotourism”, “food production” and “social behavior” play a crucial role in achieving Iran's Vision Policy of 1404 goals. However, Iran's valuable position in ornamental fish farming industry has been less attention and several issues challenged its development trend. Markazi is one of the leading provinces in this industry. So the main purposes of research were to identify current situation and providing development strategies. This study was descriptive and data were collected by survey method. The Statistical population was ornamental fish’s Producers and experts in Markazi province which due to limit number, census sampling was applied. The main tool for gathering information was questionnaire which its face validity confirmed through educational specialist and expert’s viewpoint. Also, the reliability of research tool was evaluated by Coronbach Alfa Test. The results showed that "Employment and income" and "Interest in breeding ornamental fish" were two main reasons to attract practitioners to this industry. The respondents considered the current situation ornamental fish industry, undesirable (Score 1.17 out of 5). Factor analysis showed that five factors named; "Corporate management and food resources", "management of extension and informing about authentic breeding principles", "Care Management and Insurance", "imported fish quality management" and "technical and financial support" have explained about 80% of the variance of the current situation and barriers to the development of ornamental fish industry in Markazi Province. The most important strategies to reduce facing problem in mentioned industry were development priority belonged ornamental fish industry trade system and development of specialized human resources training

Effect of synthetic and algal astaxanthin levels on egg astaxanthin content of rainbow trout (Oncorhynchus mykiss)

This research was done in a trout farm in Kohkiloyeh and Boyer- Ahmad province. The main object of this study was comparing of two sources of astaxanthin (synthetic or algae) in feed on astaxanthin content of egg in rainbow trout. It was considered seven groups consisting six treatments (T1-T6) in two different astaxanthin sources and a control (C) (without astaxanthin). So, algal astaxanthin (haematococcus pluvialis) in the three levels of 2.67, 3.55 and 8gr/kg food (T1,T2, T3); and synthetic astaxanthin in three levels of 40, 80 and 120mg/kg food in diet (T4, T5, T6) examined on 140 trout broods (3-4 years) for 4 months, before the spawning season. Astaxanthin content of obtained eggs from all treatments in spawning season was measured by HPLC apparatus. The highest and the lowest amount of egg astaxanthin were observed in T3 and C respectively. In each astaxanthin group, a significant difference was obtained between averages in treatments (P <0.05), as T3 was the highest between them. No significant difference was observed between synthetic astaxanthin treatments (T4, T5 and T6) and T1 (the lowest level of algal astaxanthin). Treatments T2 and T6 also had the same function in term of saving astaxanthin in eggs. It also concluded that natural astaxanthin (Haematococcus pluvialis) for the reason that contains supplementary nutritious, is extraordinary preferable than synthetic astaxanthin to improve astaxanthin content of egg in rainbow trout

The feasibility commercialized study to produce vitamin and mineral premixes and binder from seaweed Sargassum ilicifolium for White leg shrimp

Seaweed belonged to baseborn marine plants with cell wall containing of valued polysaccharides such as Agar, Alginate, Limen sulfate and Carmine, used as food additives and in different industries. Base on previous studies, more than 1000 MT. of Sargassum seaweed withdraw from Oman Sea in Sistan and Baluchistan coastal line which can be collect, dried and powdered for using as supplement in shrimp feed. In this project, Sargassum ilicifolium collected from 6 coastal areas, rinsed, dried, powdered and measured the nutritional values in laboratory for surveying statistically. According to the high nutritional value of Tis coastal seaweed, this variety seaweed powder, replaced with protein resources (fish meal and Soy and Wheat) of white-leg shrimp feed which was formulated by Havorash feed factory of Boshehr in four treatments (A: as control without any replacement) B: with 5%, C: 10 % and D: 15% seaweed replacement, each with three replicates in order to obtain isonitrogenus 33% CP., and iso-caloric (13% fat and 15% carbohydrate) feed using winfeed software. The weighed milled ingredients were carefully mixed using a laboratory food mixer. The mixtures were primed with 30% hot water to yield a suitable pulp. Wet diets were made into 2 mm pellet size and dried at 40 °C in a drying cabinet and maintained in standard condition which was used for water stability and absorption capacity test of the pellets in sea water, statistically one way- ANOVA. The Tis coastal seaweed with 9.8% CP, 2% lipid and 23% carbohydrate had higher nutritional value compared to the other gathered seaweed. Also amino acid and fatty acid profiles, vitamins and minerals were measured in all seaweed samples each, with three replications. As result, for using Sargassum ilicifolium as vitamins and mineral premixes in white- leg shrimp feed, Zinc, Cobalt and Phenylalanine with dose of 1.1, 06.0 and 4.0 ml. 100% dried seaweed must be added respectively. The water stability of D feed treatment in seawater (98%) and C (97%) had statistical differences with A and B (95% stability) (P<0.05). Water absorption capacity of feeds after one hour immersion in seawater showed significance difference between D (110%) and three others, C(100%), B(85% and control(80%) (P<0.05). As final aim of this project, enriched product of this seaweed as minerals and vitamins supplements were handsel joinery in the Second Medicine Plant Festival, 2016 and registered in recording organization to give the final certificate

Active analysis of condition of feed and feeding in Iranian sturgeon fish larvae

In the first chapter, all documents in aquaculture feed, feed formulation, nutritional requirement and basic concepts of aquatic feeding had been collected. The second chapter focused on broodstock feeding in order to improve reproduction efficiency. The third chapter performed to importance of live food specially in sturgeon fishes larvae and the forth chapter attended to biology and ecology of this fishes and finally in the fifth chapter, special nutrition and feeding in sturgeon fish larvae had been brought with all the works out in Iran on. There are 63 documents including papers, thesis and reports from different research library and universities among them 34 base on sturgeon larvae nutrition especially on Huso huso and Persian sturgeon species and almost there are not any documents on the other three species. 14 documents comes from the role of protein, energy and lipid replacements basically in the first growth year, 3 of back to the special nutrition requirement of Persian and Huso huso species and 12 were on digestive enzymes physiology, the role of probiotic and prebiotic on growth and survival rate. Almost there is not any organization in Iran to work on this species specifically and it seems every student and researcher paid attend to a narrow way of high way sturgeon fish researches. There is not any data bank on these important fishes and some repeated worked out by researchers in different parts of the country. Beside of this documents there had been gathered some research documents from the other countries for comparing and showing the correct way in this regards. First, we must focus on broodstock sturgeon feeding, then on egg and yolk analysis, larval stages, morphology, measurement of larval mouth and finding the best live food for different stages of sturgeon aviculture and finally on digestive physiology and enzymatic activities. In the stage of shifting from endogenous to exogenous feeding, nutritional requirement, protein digestibility, essential and free amino acids, essential fatty acids specially PUFA and HUFA, vitamins, minerals, pigments, growth and survival stimulants and resistance to physiological environmental stress are very important to search

Providing database of Iranian fish larvae under Iranian fisheries research organization net work

This project prepared and implemented during 2013-16 with emphasis on to provide the results of the fish larva projects (identification, abundance and distribution) in the Iranian waters of the Persian Gulf and Gulf of Oman which as well as had already been done by Iranian Fisheries Science Research Institute and affiliated research institutes and centers . Since each marine fish stocks management requires increased knowledge of the life cycle and be complete biological information, obviously implementation and increased field research is essential, on the other hand the results and the availability of data and information to operation and coordination with the scientific community at national and international levels is of particular importance. This is on condition that currently, due to the increasing trend researchers and students Aquatic Sciences and Fisheries, the availability of data resulting from studies and research projects and initiatives will be necessary. The site is currently only in view of identification and distribution of marine fish larva has been prepared in Farsi with photo gallery, and English language development and preparation of the site for the international operation there. It is worth mentioning in the same areas and the same site adjacent seas of fish larvae has not been prepared and is not available. So this site can also be considered as reference area. This site is available for researchers, scientist and students as address: http://ifl.ifro.ir

Quantitative and qualitative development of shrimp culture in Iran

Primary researches on aquaculture of endemic shrimp species carried out since 1984-5 by Iranian Fisheries Research Organization (IFRO) .After a decade, the condition for extension and development of shrimp culture in the country prepared. Despite huge investment of government for preparation and construction of shrimp sites, only small part of present potential in southern coast of the country is under production. About 18 different shrimp species inhabit in Persian Gulf and Sea of Oman, in which 4 species have larger size, and more abundant, and harvest commercially . Penaeus semisulcatus, Fenneropenaeus merguiensis and Metapenaeus affinis as commercial species distributed throughout of Persian Gulf from mouth of Arvand River in north- west to strait of Hormoz, and also some parts of Oman Sea. While Fenneropenaeus indicus distributed mainly in coastal area of Oman Sea. Shrimp culture begin in Iran by P.semisulcatus, continued by imported Penaeus monodon, and developed by using of F.indicus. After confront to slow growth and viral diseases in 2001 (Khouzestan province) and 2005 (Bushehr Province), at the same time I.F.R.O acquired the bio-technique for production of Litopenaeus vannamei, and hence the condition for introduction the new species to the shrimp culture industry provided. In accordance for development of shrimp culture, I.F.R.O carried out various research activities, and established specific research center for this purpose. Research projects regarding brood-stock management, breeding and larvae production, grow out, water management and disease identification and treatment have been carried, and induced considerable impacts on quantitative and qualitative development of shrimp culture in the country

Production of artificial diets and determination of their effects as alone and combination with natural wet diet on female western white shrimp broodstock maturation (Litopenaeus vannamei)

Due to the usefulness of shrimp broodstock pelleted diets, from aspects of, easier maintenance, transportation, broodstock feeding, and cheaper as compared to natural wet diets, the use of natural wet foods, include sand worm (Perinereis nuntica), cattle fish )Sepia pharaonis) and veal livier decreased and the quantity of pelleted diet increased. Survey was conducted, in tankes with a volume of 300 liters. Tanks were filled with 150 liters of water. 10 broodstock in each tank was left, with an average weight of 37±2 grams. Daily feeding rate, was twenty-five percent of their biomass. The survey was include, 9 treatments with 3 replicates in each tank as described below. Control treatment: broodstock feeding only with, sand worm (33%), cattle fish (34%) and bull livier (33%). Exprimental treatment 1: broodstock feeding with pelleted diet contain 50 percent crude protein and 8 percent crude fat (50%)+sand worm (16 %)+cattle fish (18%)+veal livier (16%). Treatment 2: broodstock feeding with pelleted diet contain 50 percent crude protein and 10 percent crude fat (50 %)+sand worm (16 %)+cattle fish (18%) and veal livier (16%). Treatment 3: broodstock feeding with pelleted diet contain 40 percent crude protein and 10 percent crude fat (50%)+sand worm (16 %)+cattle fish (18 %) and veal livier (16 %). Treatment 4: broodstock feeding with pelleted diet contain 40 percent crude protein and 8 percent crude fat (50 %)+sand worm (16 %)+cattle fish (18 %) and veal livier (16 %). Treatment 5: broodstock feeding with pelleted diet contain 50 percent crude protein and 10 percent crude fat (100 %). Treatment 6: broodstock feeding with pelleted diet contain 50 percent crude protein and 8 percent crude fat (100 %). Treatment 7: broodstock feeding with pelleted diet contain 40 percent crude protein and 10 percent crude fat (100 %). Treatment 8: broodstock feeding with pelleted diet contain 40 percent crude protein and 8 percent crude fat (100%). The results showed that, Gonadosomatic index (GSI) in treatments 3: control and 6, was significantly more than others treatments (p0.05). From the aspect of mean weight of broodstock, wasn’t significant difference in treatments (p>0.05). From the aspect of mean length of carapac, wasn’t significant difference in treatments (p>0.05). From the aspect of mean body length, wasn’t significant difference in often treatments (p>0.05), and in treatments 5 and 6 was significantly less than others (p0.05). In the determination of, correlation between weight (g) and total length(cm), (r=0.71), weight and carapace length (cm) (r=0.70), the correlation was strong. Between GSI, HIS, carapace length and total length the correlation was intermediate (r=0.54). The correlation between absolutely fecundity and total length (r=0.20), absolutely fecundity and carapace length (r=0.28), absolutely fecundity and weight (r=0.35) was weak. The results showed that, the use of combination of pelleted diet and natural wet diets can increase female maturation indexes. Totally we can noted that, GSI, HIS and absolute fecundity of broodstock, that fed with pelleted diet contain 40 percent crude protein and 10 percent crude fat (50 %)+sand worm (16 %)+cattle fish (18 %) and veal livier (16 %) (treatment 3) was better than the other treatments. Positive effects of this treatnent on sexual indexes, was due to provide part of nutritional requirement of shrimp broodstock from pelleted diet

Aquaculture potentials investigation of Yamchi Dam Down stream areas in in Ardabil Province

The research conducted for finding of aquaculture potential and appropriate areas of Yamchi dam downstream river and estimate of its production in 2010-2012. Yamchi dam is located at 25 kilometers of southwest of the Ardabil city. This research done by using of available information and data obtained from the monthly and seasonally sampling. After evaluating of Yamchi river potential and its marginal lands, suitable locations for aquaculture determinated and theirs production was calculated at each site. Totally, 3 areas selected for trout aquaculture and varius method suggested including race way, octagonal pond and semi-recircultaion. In the present condition of Yamchi river the amount of trout production is predicted at least 65 ton by race way to maximum 1750 ton by semirecircultaion method. Whiles, if 2 cubic meters of water from Yamchi dam outlet is provided to aquaculture, it will be predicted at least 580 ton by race way to maximum 4000 ton by semi-recircultaion metho

The survey of investigation about Mesopotamichthys sharpeyi projects in development program in Iranian Fisheries Research

Attention to the aquaculture capacity in inland water is needed to reach production of 262072 tons which was implicited in sixth economical and social development program of culture and production warmwater fishes industry. On of the most important aim of progressive management in fisheries program is native aquaculture activity with emphasis on Barbus fishes in Iran.Benni fish is one of the species of this genus despit of low relative growth rate, but is as a desire fishes in market. This fish not only has high fecondity and feed phytoplankton during the larval stages but also is very delicious for consumers. During the fifth economical and social development program there were so many projects have been done on aquaculture of this species but needs to analysis of all data which is important for successive future its aquaculture commercially. The precent analytical investigation base on three decades data, was done for completeing that gap including of analytical activities of researches were done, developmental access to fish aqu culture in six proficiency subjects , propagation and cultue, nutrition, genetic, health and disease biology, physiologt and determination of weak points. Then base on guideline studies on warmwater aquaculture and developmental road map, several suggestion for doing apply research were adopted