X-ray Variability and Hardness of ESO 243-49 HLX-1: Clear Evidence for Spectral State Transitions

The ultra-luminous X-ray (ULX) source ESO 243-49 HLX-1 currently provides the strongest evidence for the existence of intermediate mass black holes. We conduct an ongoing monitoring campaign with the Swift X-ray Telescope and found that HLX-1 showed two fast rise and exponential decay with increases in the count rate of a factor ~40 separated by 375+/-13 days. We obtained new XMM-Newton and Chandra dedicated pointings that were triggered at the lowest and highest luminosities, respectively. The unabsorbed luminosities ranged from 1.9x10^40 to 1.25x10^42 erg/s. We confirm here the detection of spectral state transitions from HLX-1 reminiscent of Galactic black hole binaries: at high luminosities, the X-ray spectrum showed a thermal state dominated by a disk component with temperatures of 0.26 keV at most, and at low luminosities the spectrum is dominated by a hard power law with a photon index in the range 1.4-2.1, consistent with a hard state. The source was also observed in a steep power law state. In the thermal state, the luminosity of the disk component appears to scale with the fourth power of the inner disk temperature which supports the presence of an optically thick, geometrically thin accretion disk. The low fractional variability (rms of 9+/-9%) in this state also suggests the presence of a dominant disk. The spectral changes and long-term variability of the source cannot be explained by variations of the beaming angle and are not consistent with the source being in a super-Eddington accretion state. HLX-1 is thus an unusual ULX as it is similar to Galactic black hole binaries, which have non-beamed and sub-Eddington emission, but with luminosities 3 orders of magnitude higher. In this picture, a lower limit on the mass of the black hole of >9000 M_sun can be derived, and the disk temperature in the thermal state also suggests the presence of a black hole of a few 10^3 M_sun.Comment: accepted for publication in ApJ (October 10, 2011, v740-1); 11 figures, 13 pages with emulateapj styl

Transcriptional and Functional Analysis of the Effects of Magnolol: Inhibition of Autolysis and Biofilms in Staphylococcus aureus

BACKGROUND: The targeting of Staphylococcus aureus biofilm structures are now gaining interest as an alternative strategy for developing new types of antimicrobial agents. Magnolol (MOL) shows inhibitory activity against S. aureus biofilms and Triton X-100-induced autolysis in vitro, although there are no data regarding the molecular mechanisms of MOL action in bacteria. METHODOLOGY/PRINCIPAL FINDINGS: The molecular basis of the markedly reduced autolytic phenotype and biofilm inhibition triggered by MOL were explored using transcriptomic analysis, and the transcription of important genes were verified by real-time RT-PCR. The inhibition of autolysis by MOL was evaluated using quantitative bacteriolytic assays and zymographic analysis, and antibiofilm activity assays and confocal laser scanning microscopy were used to elucidate the inhibition of biofilm formation caused by MOL in 20 clinical isolates or standard strains. The reduction in cidA, atl, sle1, and lytN transcript levels following MOL treatment was consistent with the induced expression of their autolytic repressors lrgA, lrgB, arlR, and sarA. MOL generally inhibited or reversed the expression of most of the genes involved in biofilm production. The growth of S. aureus strain ATCC 25923 in the presence of MOL dose-dependently led to decreases in Triton X-100-induced autolysis, extracellular murein hydrolase activity, and the amount of extracellular DNA (eDNA). MOL may impede biofilm formation by reducing the expression of cidA, a murein hydrolase regulator, to inhibit autolysis and eDNA release, or MOL may directly repress biofilm formation. CONCLUSIONS/SIGNIFICANCE: MOL shows in vitro antimicrobial activity against clinical and standard S. aureus strains grown in planktonic and biofilm cultures, suggesting that the structure of MOL may potentially be used as a basis for the development of drugs targeting biofilms

Efficacy of modified thoraco-laparoscopic Ivor-Lewis versus traditional thoraco-laparoscopic Ivor-Lewis for esophageal cancer: Propensity score-matched analysis

ObjectiveTo compare the clinical results of the modified Ivor-Lewis procedure, which preserves the azygous vein, thoracic duct and surrounding tissues, with the traditional Ivor-Lewis procedure, which removes these tissues, for treating esophageal squamous cell carcinoma, and evaluating whether the azygous vein, thoracic duct and surrounding tissues are required to be removed for the surgery of esophageal cancer.MethodsTo retrospectively analyze the clinical data of patients suffering from esophageal cancer treated by thoracic-laparoscopic Ivor-Lewis procedure admitted to the Department of Thoracic Surgery of Gansu Provincial People’s Hospital from September 2017 to September 2019. According to the surgical method, they were divided into the modified thoracolaparoscopic Ivor-Lewis (modified group) and the traditional thoracolaparoscopic Ivor-Lewis (traditional group). Propensity score matching analysis (PSM) was applied to reduce the selection bias of confounding factors.ResultsA total of 245 patients who suffered from esophageal cancer and underwent thoracic-laparoscopic Ivor-Lewis were enrolled in the study. There were 124 cases in the modified group and 121 cases in the traditional group. The discrepancies in the age and T-stage among patients in the traditional and modified groups were statistically significant. After PSM, the above-mentioned factors became statistically insignificant. There were 86 patients in each group after PSM. Compared with the traditional group, the modified group has shorter operative time (p=0.007), less intraoperative bleeding (p=0.003) and less postoperative 3 days chest drainage(p=0.001), with a statistically significant difference. No significant difference in local recurrence (p=0.721) and distant metastasis (p=0.742) after surgery were found in the two groups, and the difference was not statistically significant. There was also no statistically significant difference in the 3-year postoperative survival rate (44.2% vs. 41.9%, p=0.605) between the modified and traditional groups.ConclusionThe modified Ivor-Lewis procedure, which preserves the azygous vein, thoracic duct, and surrounding tissue, reduces surgical trauma in esophageal cancer, has not increased postoperative recurrent metastases, while achieved the same long-term outcomes as expanded surgery

An Inhibitory Effect of Dryocrassin ABBA on Staphylococcus aureus vWbp That Protects Mice From Pneumonia

Von Willebrand factor-binding protein (vWbp), secreted by Staphylococcus aureus (S. aureus), can activate host prothrombin, convert fibrinogen to fibrin clots, induce blood clotting, and contribute to pathophysiology of S. aureus-related diseases, including infective endocarditis, staphylococcal sepsis and pneumonia. Therefore, vWbp is an promising drug target in the treatment of S. aureus-related infections. Here, we report that dryocrassin ABBA (ABBA), a natural compound derived from Dryopteris crassirhizoma, can significantly inhibit the coagulase activity of vWbp in vitro by directly interacting with vWbp without killing the bacteria or inhibiting the expression of the vWbp. Using molecular dynamics simulations, we demonstrate that ABBA binds to the “central cavity” in the elbow of vWbp by interacting with Arg-70, His-71, Ala-72, Gly-73, Tyr-74, Glu-75, Tyr-83, and Gln-87 in vWbp, thus interfering with the binding of vWbp to prothrombin. Furthermore, in vivo studies demonstrated that ABBA can attenuate injury and inflammation of mouse lung tissues caused by S. aureus and increase survival of mice. Together these findings indicate that ABBA is a promising lead drug for the treatment of S. aureus-related infections. This is the first report of potential inhibitor which inhibit the coagulase activity of vWbp by directly interacting with vWbp

Cortical Gyrification and Sulcal Spans in Early Stage Alzheimer's Disease

Alzheimer's disease (AD) is characterized by an insidious onset of progressive cerebral atrophy and cognitive decline. Previous research suggests that cortical folding and sulcal width are associated with cognitive function in elderly individuals, and the aim of the present study was to investigate these morphological measures in patients with AD. The sample contained 161 participants, comprising 80 normal controls, 57 patients with very mild AD, and 24 patients with mild AD. From 3D T1-weighted brain scans, automated methods were used to calculate an index of global cortex gyrification and the width of five individual sulci: superior frontal, intra-parietal, superior temporal, central, and Sylvian fissure. We found that global cortex gyrification decreased with increasing severity of AD, and that the width of all individual sulci investigated other than the intra-parietal sulcus was greater in patients with mild AD than in controls. We also found that cognitive functioning, as assessed by Mini-Mental State Examination (MMSE) scores, decreased as global cortex gyrification decreased. MMSE scores also decreased in association with a widening of all individual sulci investigated other than the intra-parietal sulcus. The results suggest that abnormalities of global cortex gyrification and regional sulcal span are characteristic of patients with even very mild AD, and could thus facilitate the early diagnosis of this condition

Carry trade and its relationship with the stock market: Evidence from New Zealand

This study investigates the New Zealand dollar carry trade and its effect on the New Zealand stock market. Using a Vector Autoregression (VAR) model, the Granger causality relationship is from carry trade to stock market. The US dollar, Euro and Swiss Franc dominate carry trade as funding currencies and the New Zealand Dollar as investment currency. There is no evidence of Japanese Yen and New Zealand Dollar carry trade during the sample period of 2007 to 2017. Carry trade returns’ effect on New Zealand stock market sector returns are generally attributed with various degree and preference. The basic materials sector is the only exception, where there is no Granger causality relationship in either direction. It also indicates carry trade returns positively affect the New Zealand stock market in both periods of crisis and post crisis. However, the Granger causality relationship is stronger in crisis period than it is in post crisis period

Performance evaluation of intermittent air supply system: a case study in Xi’an

Intermittent air jet strategy (IAJS) has been proven to provide a satisfactory indoor thermal climate with high energy efficiency in spaces with high occupancy density. Compared with traditional ventilation strategy, IAJS system creates heterogeneous conditions around the occupants, which complicates the relationship between thermal sensation and thermal comfort. The effect of different intermittent cycles on the thermal comfort of occupants needs to be further discussed. In addition, it is necessary to investigate whether IAJS system can play a better role in hot and humid summer conditions in China. Experiments were conducted in a chamber with two rows of seated occupants. Twelve cases were designed, including different combinations of room air temperatures (24°C, 26°C and 28°C) and intermittent period (start 90 s stop 90 s, start 3 min stop 1.5 min, start 1.5 min stop 3 min and start 3 min stop 3 min). Twelve subjects were recruited for subjective investigations. The subjects performed sedentary work and wore summer clothing. Subjective responses to the thermal environments were collected. Wind speed, temperature and CO2 concentration in the occupied area were measured. The results show that IAJS has a potential to be used as a primary ventilation system primary ventilation system such as classrooms in China. When the indoor air was set between 24°C and 26°C, IAJS could create a thermal climate that made the occupants thermally neutral. Additionally, IAJS created an indoor thermal environment acceptable to most occupants, even at a setting temperature of 28 °C