USE OF COMBINATION OF FLUORESCENT PROBES TO IDENTIFY SPERM SUBPOPULATIONS FOR QUALITY ASSESSMENT OF FRESH AND CRYOPRESERVED CANINE SEMEN. PRELIMINARY RESULTS

The use of fluorescent markers in the evaluation of sperm morphophysiology allows a better accuracy, compared to the subjective nature of some routine tests in semen qualification. In this study was used the combination of fluorescence probes: propionate iodide, Hoechst 33342 and FITC-PSA in fresh and thawed dog semen, to the identification of the following morphological subpopulations: II (intact plasma and acrosomal membranes), IL (intact plasma membrane and lesioned acrosomal membrane), LI (lesioned plasma membrane and intact acrosomal membrane) and LL (both membranes lesioned). When comparing the results obtained with the results of the tests used conventionally in semen evaluation (sperm motility and vigor, hypoosmotic test and morphological alterations), little correlation was observed. The II population declined from fresh semen to thawed, while LL population increased (p <0.05). The IL population was composed of extremely small numbers of cells but increased (p <0.05) from fresh semen to thawed semen. In the thawed semen the major defects had a positive correlation with the LL population (p <0.01). For the thawed semen, the results of the hypoosmotic test (number of cells that reacted to the medium) correlated positively with population II (p <0.025), that is, different from that observed in fresh semen. Although all tests were able to detect decrease in sperm quality post-thawing (p <0.05). The use of this fluorescent probe association allowed qualification and more accurately quantification of plasma membrane and acrosomal insults mediated by cryopreservation. El uso de marcadores fluorescentes en la evaluación de la morfofisiología espermática permite una mayor precisión, comparada con la naturaleza subjetiva de algunas pruebas de rutina en la valoración del semen. En este estudio se usó la combinación de pruebas fluorescentes: yoduro de propidio; Hoechst 33342 y FITC-PSA en semen fresco y descongelado de perro, para la identificación de las siguientes subpoblaciones morfológicas: II (membranas plasmática y acrosomal intactas), IL (membrana plasmática intacta y membrana acrosomal dañada), LI (membrana plasmática dañada y membrana acrosomal intacta) y LL (ambas membranas dañadas). Cuando se comparan los resultados obtenidos con los resultados de las pruebas usadas convencionalmente en la evaluación seminal (motilidad y vigor espermáticos, prueba hipoosmótica y alteraciones morfológicas), se observó poca correlación. La población II disminuyó desde el semen fresco al descongelado, mientras que la población LL se incrementó (p<0.05). la población IL estuvo compuesta por un número extremadamente pequeño de células, pero incremento (p<0.05) desde el semen fresco al descongelado. En el semen descongelado los defectos mayores tuvieron una correlación positiva con la población LL (p<0.01). En el semen descongelado, los resultados de la prueba hipoosmótica (número de células que reaccionan al medio) se correlacionaron positivamente con la población II (p<0.05). El uso de esta asociación de pruebas fluorescentes permitió la valoración y la cuantificación más precisa de los daños a la membrana plasmática y acrosomal mediados por la criopreservación.

Cryopreservation of immature bovine oocytes with 1,2 propanediol

O objetivo deste trabalho foi avaliar o efeito de dois gradientes de concentrações de 1,2 propanediol (PROH) na criopreservação de ovócitos imaturos, envoltos em células do cumulus, de bovinos abatidos em matadouro. No tratamento 1, com uso de 1,6 M de PROH, os ovócitos foram desidratados em três etapas crescentes (0,53; 1,06 e 1,6 M), à temperatura ambiente e congelados em nitrogênio líquido. A descongelação foi realizada em água a 37ºC por 30 segundos e a reidratação em três etapas decrescentes (1,6; 1,06 e 0,53 M), acrescidos de 0,25 M de sacarose cada. No tratamento 2 foi utilizado 2,0 M de PROH, de maneira semelhante ao tratamento 1, com desidratação contendo 0,7; 1,4 e 2,0 M, e reidratação 2,0; 1,4 e 0,7 M. Após a reidratação, os ovócitos foram lavados em meio TCM 199 (Meio de Cultivo para Tecidos) e levados para maturação in vitro. O grupo controle foi constituído de ovócitos recém-colhidos. Os ovócitos foram colocados para maturar por 24 horas, em meio TCM 199 com 10% de SVC (Soro de Vaca em Cio) e FSH (Hormônio Estimulante de Folículo), em co-cultura com células da granulosa, com 5% CO2 no ar a 39ºC. Os resultados de maturação nuclear foram diferentes (P<0,05) entre os ovócitos criopreservados e controle, sendo 2,04%, 0% e 84% para o tratamento 1, 2 e controle, respectivamente.A study was carried out to investigate the ability of immature bovine cumulus-oocytes complexes to survive cryopreservation and undergo subsequent in vitro maturation in two different levels of propanediol (PROH). Treatment 1 consisted of subjecting oocytes to a gradual 3-step, concentration-driven dehydration with PROH (0.53; 1.06 and 1.6 M) at room temperature and subsequent freezing. Oocytes were thawed in water at 37ºC for 30 seconds and subjected to a gradual 3-step, concentration-driven rehydration with PROH (1.6; 1.06 and 0.53 M) in the presence of 0.25 M sucrose. Treatment 2 was conducted the same way, but PROH concentrations for dehydration were 0.7; 1.4 and 2.0 M and for rehydration were 2.0; 1.4 and 0.7 M. After rehydration, oocytes of both treatments were washed in TCM 199 medium followed by the in vitro maturation. The control consisted of nonfrozen oocytes. Oocytes of all groups were cultured in TCM 199 medium with 10% ECS and FSH, and 5% CO2 air in co-culture at 39ºC, for 24 hours. The in vitro maturation rate of cryopreserved oocytes were different for treatments and control. Metafase II was reached by 2.04%, 0% and 84% for treatment 1, 2 and control, respectively

Estrógeno exógeno no início do ciclo estral de vacas leiteiras na indução do estro e na dinâmica ovariana

O objetivo do presente trabalho foi verifi car o efeito do estrógeno exógeno, em fase precoce do ciclo estral, de vacas leiteiras, na indução do comportamento (sinais) de estro e na dinâmica ovariana. Foram utilizadas 16 vacas mestiças holandês-zebu ciclando regularmente, sem apresentar qualquer alteração clínica ou reprodutiva. As vacas foram incluídas ao acaso nos respectivos tratamentos. Tratamento 1: oito vacas receberam 2,5mL de cipionato de estradiol no 1º dia do ciclo estral, (considerando dia 0 o dia do estro). Tratamento 2 (controle): oito vacas sem tratamento. A manifestação de estro foi monitorada visualmente. Os exames ultrassonográfi cos foram realizados diariamente pela manhã, iniciando no dia do estro. As coletas de sangue para as dosagens de progesterona tiveram início no dia do estro (dia 0) e foram realizadas a cada três dias até o próximo estro. Os animais que receberam estrógeno no 1o dia após o estro natural manifestaram os sinais característicos de estro (psíquicos, útero túrgido e muco abundante) um dia após o tratamento. Esta redução do intervalo de estro para apenas dois dias pode favorecer a eliminação de bactérias do útero (quando presentes), aumentando, consequentemente, a eficiência reprodutiva. Excetuando o dia da emergência da 1a onda folicular, todas as outras variáveis estudadas (número e comprimento de ondas, características dos folículos dominantes e subordinados, assim como os parâmetros relacionados ao corpo lúteo e produção de progesterona) não foram afetadas pela aplicação de estrógeno um dia após o estro. A aplicação de cipionato de estradiol em vacas mestiças, um dia após o estro natural promove o aparecimento de sinais deestro (psíquicos, útero túrgido e muco abundante) dois dias após o estro natural, sem afetar as características do ciclo estral subsequente

Thrombus aspiration in patients with ST-elevation myocardial infarction: results of a national registry of interventional cardiology

BACKGROUND: We aimed to evaluate the impact of thrombus aspiration (TA) during primary percutaneous coronary intervention (P-PCI) in 'real-world' settings. METHODS: We performed a retrospective study, using data from the National Registry of Interventional Cardiology (RNCI 2006-2012, Portugal) with ST-elevation myocardial infarction (STEMI) patients treated with P-PCI. The primary outcome, in-hospital mortality, was analysed through adjusted odds ratio (aOR) and 95% confidence intervals (95%CI). RESULTS: We assessed data for 9458 STEMI patients that undergone P-PCI (35% treated with TA). The risk of in-hospital mortality with TA (aOR 0.93, 95%CI:0.54-1.60) was not significantly decreased. After matching patients through the propensity score, TA reduced significantly the risk of in-hospital mortality (OR 0.58, 95%CI:0.35-0.98; 3500 patients). CONCLUSIONS: The whole cohort data does not support the routine use of TA in P-PCI, but the results of the propensity-score matched cohort suggests that the use of selective TA may improve the short-term risks of STEMI..info:eu-repo/semantics/publishedVersio

Insatisfação com a imagem corporal, uso de drogas e fatores associados entre adolescentes em três cidades brasileiras

Objetivo:&nbsp;analisar a associação entre uso de drogas e a insatisfação com a imagem corporal de adolescentes em três cidades brasileiras.&nbsp;Método:&nbsp;estudo transversal, aninhado em um ensaio controlado randomizado para avaliar o programa de prevenção ao uso de drogas em escolas brasileiras&nbsp;#TamoJunto2.0 do Ministério da Saúde. A amostra foi de 5.213 alunos de 73 escolas de três cidades brasileiras. O desfecho satisfação corporal foi analisado por meio da escala de Silhuetas de Stunkard e as variáveis explicativas foram o uso de drogas e dados sociodemográficos.&nbsp;Resultados:&nbsp;os adolescentes tinham entre 12 e 14 anos e cerca de 69,9% relataram insatisfação corporal, e no último ano 35,67% utilizaram álcool. A insatisfação por sobrepeso foi maior nas meninas (41,5%) e por baixo peso nos meninos (33,1%). Os adolescentes que usavam maconha apresentaram 39% (OR=1,39) mais chance de insatisfação por baixo peso e ser menina aumentou em 24% (OR=1,24) as chances de insatisfação por sobrepeso.&nbsp;Conclusão:&nbsp;os níveis de insatisfação corporal merecem atenção no cuidado de enfermagem hebiátrica e reforçam a necessidade de estratégias educativas abordando a imagem corporal e uso de drogas, a relacionando aos vários atributos subjetivos que podem afetar a saúde dos adolescentes, seja na comunidade ou escola.Objective:&nbsp;analyze the association between drug use and body dissatisfaction among adolescents in three Brazilian cities.&nbsp;Method:&nbsp;cross-sectional study, using a nested randomized controlled trial to evaluate the drug use prevention program&nbsp;#TamoJunto2.0 of the Ministry of Health in Brazilian schools. The sample consisted of 5,213 students from 73 schools in three Brazilian cities. The outcome body satisfaction was analyzed using the Stunkard scale and the explanatory variables were drug use and sociodemographic data.&nbsp;Results:&nbsp;the adolescents were between 12 and 14 years old; about 69.9% of them reported body dissatisfaction, and 35.67% used alcohol in the previous year. Dissatisfaction due to overweight was higher among girls (41.5%) and dissatisfaction due to underweight was higher among boys (33.1%). Adolescents who used marijuana were 39% (OR=1.39) more likely to feel dissatisfied due to underweight and being a girl increased the chances of feeling dissatisfied due to overweight by 24% (OR=1.24).&nbsp;Conclusion:&nbsp;the levels of body dissatisfaction deserve attention in hebiatric nursing care and reinforce the importance of educational strategies addressing body image and drug use, relating them to the various subjective attributes that can affect the health of adolescents, whether in the community or at school.Objetivo:&nbsp;analizar la asociación entre uso de drogas y la insatisfacción con la imagen corporal de adolescentes en tres ciudades brasileñas.&nbsp;Método:&nbsp;estudio transversal, anidado en un ensayo controlado aleatorio para evaluar el programa de prevención del uso de drogas en escuelas brasileñas&nbsp;#TamoJunto2.0 del Ministerio de la Salud. La muestra fue de 5.213 alumnos de 73 escuelas de tres ciudades brasileñas. El desenlace satisfacción corporal se analizó mediante la escala de Siluetas de Stunkard y las variables explicativas fueron el consumo de drogas y los datos sociodemográficos.&nbsp;Resultados:&nbsp;los adolescentes tenían entre 12 y 14 años y alrededor del 69,9% relató insatisfacción corporal, y en el último año el 35,67% consumió alcohol. La insatisfacción por sobrepeso fue mayor en las niñas (41,5%) y por bajo peso en los niños (33,1%). Los adolescentes que consumían marihuana tenían un 39% (OR=1,39) más de probabilidades de estar insatisfechos con el bajo peso y ser niña aumentó en un 24% (OR=1,24) las chances de insatisfacción por sobrepeso.&nbsp;Conclusión:&nbsp;los niveles de insatisfacción corporal merecen atención en el cuidado de enfermería hebiátrica y refuerzan la necesidad de estrategias educativas abordando la imagen corporal y el uso de drogas, relacionándola a los varios atributos subjetivos que pueden afectar la salud de los adolescentes, ya sea en la comunidad o en la escuela

NITROGEN DOSES FOR COMMON BEANS CROP IN THE CERRADO REGION IN THE STATE OF BAHIA

Common beans crop is very demanding in nutrients, where nitrogen is the most required by the crop. The nitrogen fertilization recommendations for the Cerrado in Western Bahia are based on recommendations elaborated for the Cerrado conditions in the Brazilian Central-West region, and such recommendations may not be adequate, as the Cerrado in the state of Bahia is predominantly characterized by sandy soils and little rainfall than the others areas of this biome, showing peculiar characteristics regarding the behavior of nitrogen in the soil. Thus, the objective of this work was to evaluate the effect of nitrogen doses on the morphological characteristics, yield and quality of beans in the common beans crop in the Cerrado in the State of Bahia. The experiment was carried out in the municipality of Barreiras, State of Bahia (BA), in a randomized block design, consisting of five nitrogen doses (0, 60, 120, 180 and 240 kg ha-1) and four replications. The morphological attributes, grain yield and dry biomass and the physical and chemical quality of the grains were evaluated. The data were subjected to analysis of variance and the means were compared by the test of Tukey at 5% and regression analysis was performed to assess the effects of N doses on the variables. Regarding morphology, the highest nitrogen doses had a positive influence on stem diameter and number of leaves. The maximum efficiency dose was 63 kg ha-1 with productivity of 2,889.6 kg ha-1 of grains, while the maximum dose of 240 kg ha-1 increased the protein content in grains by 46% in relation to the absence of nitrogen fertilization.Common beans crop is very demanding in nutrients, where nitrogen is the most required by the crop. The nitrogen fertilization recommendations for the Cerrado in Western Bahia are based on recommendations elaborated for the Cerrado conditions in the Brazilian Central-West region, and such recommendations may not be adequate, as the Cerrado in the state of Bahia is predominantly characterized by sandy soils and little rainfall than the other areas of this biome, showing peculiar characteristics regarding the behavior of nitrogen in the soil. Thus, the objective of this study was to evaluate the effect of nitrogen doses on the morphological characteristics, yield and quality of beans in the common beans crop in the Cerrado in the State of Bahia. The experiment was conducted in the municipality of Barreiras, State of Bahia (BA), in a randomized block design, consisting of five nitrogen doses (0, 60, 120, 180 and 240 kg ha-1) and four replications. The morphological attributes, grain yield and dry biomass and the physical and chemical quality of the grains were evaluated. The data were subjected to analysis of variance and the means were compared by the test of Tukey at 5% and regression analysis was performed to assess the effects of N doses on the variables. Regarding morphology, the highest nitrogen doses had a positive influence on stem diameter and number of leaves. The maximum efficiency dose was 63 kg ha-1 with productivity of 2,889.6 kg ha-1 of grains, while the maximum dose of 240 kg ha-1 increased the protein content in grains by 46% in relation to the absence of nitrogen fertilization