Utilization Of Goose Muscle In The Preparation Of Meat Rolls

South Dakota produces more domestic geese than any other state in the United States. Marketing of geese is decreasing due to importation of Canadian geese and a decreasing consumer demand. Research is needed to increase goose meat consumption. A large percentage of turkey meat is sold as retail convenience products such as rolls, yet there are virtually no comparable products on the market from goose meat. This study was undertaken to develop and evaluate an acceptable goose roll

Utilization Of Spent Fowl In the Manufacture Of Chicken Restructured Steaks

Each year the poultry industry is faced with a large number of spent laying hens (spent fowl), which are often difficult to market at a reasonable price. If less desirable poultry carcasses such as spent hens could be utilized economically to create desirable new products, there would be considerable incentive to do so. Because of the maturity level of spent hens, their muscles are quite tough and, therefore, products made from them would have to be comminuted. A new method of comminution, called flaking, cleanly cuts frozen muscle into wafer—thin slices which aids in the binding properties upon further processing. The manufacture of restructured steaks involves, first, the flaking of meat and, secondly, the pressing of meat into a particular shape. The resulting product should be tender yet simulate the actual eating quality of a real steak. The objective of this study was to determine the optimum levels of white and dark meat required in the formulation of restructured steaks and to examine the feasibility of adding skin and fat to these products

Fabrication Of Steaks From Spent Hens

Research at SDSU has demonstrated that meat from spent laying hens (spent fowl) can be utilized in the production of fabricated steaks and/or roasts. Previous studies utilized raw meat from carcasses that were manually deboned. Under commercial deboning, meat that has been precooked is more easily separated from the bone. Precooking reduces the ability for meat to hold together or bind in a processed product. Precooked and raw spent fowl muscles were compared as raw material for the production of fabricated steaks. The objective of this research was to produce from precooked spent fowl muscle a palatable restructured product that will withstand handling, cooking and serving

Polyphenols act synergistically with doxorubicin and etoposide in leukaemia cell lines

The study aimed to assess the effects of polyphenols when used in combination with doxorubicin and etoposide, and to determine whether polyphenols sensitised leukaemia cells, causing inhibition of cell proliferation, cell cycle arrest and induction of apoptosis. This study is based on findings in solid cancer tumours, which have shown that polyphenols can sensitize cells to chemotherapy, and induce apoptosis and/or cell-cycle arrest. This could enable a reduction of chemotherapy dose and off-target effects, whilst maintaining treatment efficacy. Quercetin, apigenin, emodin, rhein and cis-stilbene were investigated alone and in combination with etoposide and doxorubicin in two lymphoid and two myeloid leukaemia cells lines. Measurements were made of ATP levels (using CellTiter-Glo assay) as an indication of total cell number, cell cycle progression (using propidium iodide staining and flow cytometry) and apoptosis (NucView caspase 3 assay and Hoechst 33342/propidium iodide staining). Effects of combination treatments on caspases 3, 8 and 9 activity were determined using Glo luminescent assays, glutathione levels were measured using the GSH-Glo Glutathione Assay and DNA damage determined by anti-γH2AX staining. Doxorubicin and etoposide in combination with polyphenols synergistically reduced ATP levels, induced apoptosis and increased S and/or G2/M phase cell cycle arrest in lymphoid leukaemia cell lines. However, in the myeloid cell lines the effects of the combination treatments varied; doxorubicin had a synergistic or additive effect when combined with quercetin, apigenin, emodin, and cis-stilbene, but had an antagonistic effect when combined with rhein. Combination treatment caused a synergistic downregulation of glutathione levels and increased DNA damage, driving apoptosis via caspase 8 and 9 activation. However, in myeloid cells where antagonistic effects were observed, this was associated with increased glutathione levels and a reduction in DNA damage and apoptosis. This study has demonstrated that doxorubicin and etoposide activity were enhanced by polyphenols in lymphoid leukaemia cells, however, differential responses were seen in myeloid cells with antagonistic responses seen in some combination therapies

The Effect of Varying Levels of Raw and Precooked Spent Fowl Muscle in the Manufacture of Restructured Steaks

Spent fowl muscle (raw or precooked) was flaked and formulated to include: 1) 100% raw meat; 2) 80% raw meat and 20% precooked meat; 3) 50% raw meat and 50% precooked meat; and 4) 20% raw meat and 80% precooked meat. The products were mixed, formed, pressed, and cut into restructured steaks. Steaks were evaluated for physical appearance, fat, and moisture content, cooking and textural properties, and sensory attributes. As the percentage of precooked spent fowl meat was increased in restructured steaks, the steaks appeared lighter in color, contained less moisture and more fat, and suffered greater cooking losses than restructured steaks made with a higher percentage of raw meat. In addition, as the percentage of precooked meat was increased, the restructured steaks became less tender, less cohesive, and received lower sensory scores

Binding of T and T analogs to CG base pairs in antiparallel triplexes.

The goal of this study was to address antiparallel triplex formation at duplex targets that do not conform to a strict oligopurine.oligopyrimidine motif. We focused on the ability of natural bases and base analogs incorporated into oligonucleotide third strands to bind to so-called CG inversions. These are sites where a cytosine base is present in an otherwise purine-rich strand of a duplex target. Using a 26-base-triplet test system, we found that of the standard bases, only thymine (T) shows substantial binding to CG inversions. This is quantitatively similar to the report of Beal and Dervan [Science (1991), 251, 1360-1363]. Binding to CG inversions was only slightly weaker than binding to AT base pairs. Binding of T to CG inversions was also evaluated in two other sequences, with qualitatively similar results. Six different analogs of thymine were also tested for binding to CG inversions and AT base pairs. Significant changes in affinity were observed. In particular, 5-fluoro-2'-deoxyuridine was found to increase affinity for CG inversions as well as for AT base pairs. Studies with oligonucleotides containing pyridin-2-one or pyridin-4-one suggest that thymine O4 plays a critical role in the T.CG interaction. Possible models to account for these observations are discussed