306 research outputs found
Can altering the distribution of organic matter in wetlands soils change methane emissions?
Forested mineral soil wetlands, found throughout the southeastern United States, store large amounts of carbon as woody biomass. Their anaerobic soil environments also make them hotspots for methanogenesis, leading to emissions of methane during soil respiration. Recent studies have suggested that at fine spatial scales (7.069 cm2), clumping of a labile carbon source, in dry soils, can result in lower microbial activity when compared to a uniformly distributed source. However, they also found in wetter soils (65% maximum water holding capacity), initial patterning of the carbon source didn’t affect microbial activity, presumably because the substrate was able to diffuse throughout the soil matrix. We investigated whether these results would hold for an inundated mineral wetland soil, Mhoon silt loam, at a larger spatial scale (1500 cm2) and with a natural wetland carbon source, bald cypress (Taxodium distichum) needles. We created three treatments: uniformly distributed needles (n=3), clumped needles (n=3), and no needles (n=3). Microbial activity is currently being measured, weekly, as the net CH4 flux. Microbial community composition (16S ribosomal RNA) was also measured after 2 months of incubation. At the end of our study needle mass loss will also be quantified for net decomposition.
Initial results (first three months) showed that clumped and uniform needles did not have significantly different mean CH4 emissions. However, preliminary analysis of our microbial data suggests that the composition of microbes varied between patches of needles and no needles within mesocosms for both uniform and clumped treatments. This indicates that resources may not be diffusing through the entire mesocosm uniformly in inundated conditions. In the coming weeks, we will be detailing these patch dynamics by identifying the relative abundances of specific microbial groups, methanogens and methanotrophs. We will also continue to measure weekly net CH4 gas fluxes, as we hypothesize that the effect of resource patterning on microbial activity may vary as a function of resource lability. This work has implications for restoration practices (e.g., plantings, soil conditions) in forested mineral soil wetlands, a class of wetlands that have been historically impacted by drain and fill activities
The Role of SurA PPIase Domains in Preventing Aggregation of the Outer Membrane Proteins tOmpA and OmpT
SurA is a conserved ATP-independent periplasmic chaperone involved in the biogenesis of outer-membrane proteins (OMPs). Escherichia coli SurA has a core domain and two peptidylprolyl isomerase (PPIase) domains, the role(s) of which remain unresolved. Here we show that while SurA homologues in early proteobacteria typically contain one or no PPIase domains, the presence of two PPIase domains is common in SurA in later proteobacteria, implying an evolutionary advantage for this domain architecture. Bioinformatics analysis of > 350,000 OMP sequences showed that their length, hydrophobicity and aggregation propensity are similar across the proteobacterial classes, ruling out a simple correlation between SurA domain architecture and these properties of OMP sequences. To investigate the role of the PPIase domains in SurA activity, we deleted one or both PPIase domains from E. coli SurA and investigated the ability of the resulting proteins to bind and prevent the aggregation of tOmpA (19 kDa) and OmpT (33 kDa). The results show that wild-type SurA inhibits the aggregation of both OMPs, as do the cytoplasmic OMP chaperones trigger factor and SecB. However, while the ability of SurA to bind and prevent tOmpA aggregation does not depend on its PPIase domains, deletion of even a single PPIase domain ablates the ability of SurA to prevent OmpT aggregation. The results demonstrate that the core domain of SurA endows its generic chaperone ability, while the presence of PPIase domains enhances its chaperone activity for specific OMPs, suggesting one reason for the conservation of multiple PPIase domains in SurA in proteobacteria
Biodegradation of the Alkaline Cellulose Degradation Products Generated during Radioactive Waste Disposal.
The anoxic, alkaline hydrolysis of cellulosic materials generates a range of cellulose degradation products (CDP) including α and β forms of isosaccharinic acid (ISA) and is expected to occur in radioactive waste disposal sites receiving intermediate level radioactive wastes. The generation of ISA's is of particular relevance to the disposal of these wastes since they are able to form complexes with radioelements such as Pu enhancing their migration. This study demonstrates that microbial communities present in near-surface anoxic sediments are able to degrade CDP including both forms of ISA via iron reduction, sulphate reduction and methanogenesis, without any prior exposure to these substrates. No significant difference (n = 6, p = 0.118) in α and β ISA degradation rates were seen under either iron reducing, sulphate reducing or methanogenic conditions, giving an overall mean degradation rate of 4.7×10−2 hr−1 (SE±2.9×10−3). These results suggest that a radioactive waste disposal site is likely to be colonised by organisms able to degrade CDP and associated ISA's during the construction and operational phase of the facility
Genomic Diversity among Actinomyces naeslundii strains and closely related species.
The aim of this study was to investigate and clarify the ambiguous taxonomy of Actinomyces naeslundii and its closely related species using state-of-the-art high-throughput sequencing techniques, and, furthermore, to determine whether sub-clusters identified within Actinomyces oris and Actinomyces naeslundii in a previous study by multi locus sequence typing (MLST) using concatenation of seven housekeeping genes should either be classified as subspecies or distinct species. The strains in this study were broadly classified under Actinomyces naeslundii group as A. naeslundii genospecies I and genospecies II. Based on MLST data analysis, these were further classified as A. oris and A. naeslundii. The whole genome sequencing of selected strains of A. oris (n = 17) and A. naeslundii (n = 19) was carried out using Illumina Genome Analyzer IIxe and Roche 454 allowing paired-end and single-reads sequencing, respectively. The sequences obtained were aligned using CLC Genomic workbench version 5.1 and annotated using RAST (Rapid Annotation using Subsystem Technology) release version 59 accessible online. Additionally, genomes of seven publicly available strains of Actinomyces (k20, MG1, c505, OT175, OT171, OT170, and A. johnsonii) were also included. Comparative genomic analysis (CGA) using Mauve, Progressive Mauve, gene-by-gene, Core, and Pan Genome, and finally Digital DNA-DNA homology (DDH) analysis was carried out. DDH values were obtained using in silico genome–genome comparison. Evolutionary analysis using ClonalFrame was also undertaken. The mutation and recombination events were compared using chi-square test among A. oris and A. naeslundii isolates (analysis methods are not included in the study). CGA results were consistent with previous traditional classification using MLST. It was found that strains of Actinomyces k20, MG1, c505, and OT175 clustered in A. oris group of isolates, while OT171, OT170, and A. johnsonii appeared as separate branches. Similar clustering to MLST was observed for other isolates. The mutation and recombination events were significantly higher in A. oris than A. naeslundii, highlighting the diversity of A. oris strains in the oral cavity. These findings suggest that A. oris forms six distinct groups, whereas A. naeslundii forms three. The correct designation of isolates will help in the identification of clinical Actinomyces isolates found in dental plaque. Easily accessible online genomic sequence data will also accelerate the investigation of the biochemical characterisation and pathogenesis of this important group of micro-organisms
Carboxyhaemoglobin levels and their determinants in older British men
Background: Although there has been concern about the levels of carbon monoxide exposure, particularly among older people, little is known about COHb levels and their determinants in the general population. We examined these issues in a study of older British men.Methods: Cross-sectional study of 4252 men aged 60-79 years selected from one socially representative general practice in each of 24 British towns and who attended for examination between 1998 and 2000. Blood samples were measured for COHb and information on social, household and individual factors assessed by questionnaire. Analyses were based on 3603 men measured in or close to (< 10 miles) their place of residence.Results: The COHb distribution was positively skewed. Geometric mean COHb level was 0.46% and the median 0.50%; 9.2% of men had a COHb level of 2.5% or more and 0.1% of subjects had a level of 7.5% or more. Factors which were independently related to mean COHb level included season (highest in autumn and winter), region (highest in Northern England), gas cooking (slight increase) and central heating (slight decrease) and active smoking, the strongest determinant. Mean COHb levels were more than ten times greater in men smoking more than 20 cigarettes a day (3.29%) compared with non-smokers (0.32%); almost all subjects with COHb levels of 2.5% and above were smokers (93%). Pipe and cigar smoking was associated with more modest increases in COHb level. Passive cigarette smoking exposure had no independent association with COHb after adjustment for other factors. Active smoking accounted for 41% of variance in COHb level and all factors together for 47%.Conclusion: An appreciable proportion of men have COHb levels of 2.5% or more at which symptomatic effects may occur, though very high levels are uncommon. The results confirm that smoking (particularly cigarette smoking) is the dominant influence on COHb levels
Analysis of the Performance of a Standardized Method for the Polishing of Methacrylic Resins
Adhesion of micro-organisms to resin surface may be caused by inadequate polishing. Most of the studies published in literature are relative to manually prepared samples and do not take into account that test repeatability is not guaranteed a priori since skills may change from one operator to another and the quality of the work done by the same expert operator may depend on “human” factors such as the level of attention, wrist trembling, etc
Altruism in a volatile world
This is the author accepted manuscript. The final version is available from Springer Nature via the DOI in this record.The evolution of altruism – costly self-sacrifice in the service of others – has puzzled biologists since
The Origin of Species. For half a century, attempts to understand altruism have been built on the insight
that altruists may help relatives to have extra offspring in order to spread shared genes . This theory –
known as inclusive fitness – is founded on a simple inequality termed ‘Hamilton’s rule’. However, explanations of altruism have typically ignored the stochasticity of natural environments, which will not
necessarily favour genotypes that produce the greatest average reproductive success. Moreover,
empirical data across many taxa reveal associations between altruism and environmental stochasticity, a pattern not predicted by standard interpretations of Hamilton’s rule. Here, we derive Hamilton’s rule
with explicit stochasticity, leading to novel predictions about the evolution of altruism. We show that of offspring produced by relatives. Consequently, costly altruism can evolve even if it has a net negative
effect on the average reproductive success of related recipients. The selective pressure on volatility
suppressing altruism is proportional to the coefficient of variation in population fitness, and is therefore diminished by its own success. Our results formalise the hitherto elusive link between bet-hedging and
altruism, and reveal missing fitness effects in the evolution of animal societies.PK was supported by the National Geographic Society (GEF-NE 145-15) and a
University of Bristol Research Studentship; ADH was supported by the Natural Environment Research
Council (NE/L011921/1); ANR was supported by a European Research Council Consolidator Grant
(award no. 682253); and SS was supported by the Natural Environment Research Council
(NE/M012913/2)
Changes in Lysozyme Flexibility upon Mutation Are Frequent, Large and Long-Ranged
We investigate changes in human c-type lysozyme flexibility upon mutation via a Distance Constraint Model, which gives a statistical mechanical treatment of network rigidity. Specifically, two dynamical metrics are tracked. Changes in flexibility index quantify differences within backbone flexibility, whereas changes in the cooperativity correlation quantify differences within pairwise mechanical couplings. Regardless of metric, the same general conclusions are drawn. That is, small structural perturbations introduced by single point mutations have a frequent and pronounced affect on lysozyme flexibility that can extend over long distances. Specifically, an appreciable change occurs in backbone flexibility for 48% of the residues, and a change in cooperativity occurs in 42% of residue pairs. The average distance from mutation to a site with a change in flexibility is 17–20 Å. Interestingly, the frequency and scale of the changes within single point mutant structures are generally larger than those observed in the hen egg white lysozyme (HEWL) ortholog, which shares 61% sequence identity with human lysozyme. For example, point mutations often lead to substantial flexibility increases within the β-subdomain, which is consistent with experimental results indicating that it is the nucleation site for amyloid formation. However, β-subdomain flexibility within the human and HEWL orthologs is more similar despite the lowered sequence identity. These results suggest compensating mutations in HEWL reestablish desired properties
Quantifying Relative Diver Effects in Underwater Visual Censuses
Diver-based Underwater Visual Censuses (UVCs), particularly transect-based
surveys, are key tools in the study of coral reef fish ecology. These
techniques, however, have inherent problems that make it difficult to collect
accurate numerical data. One of these problems is the diver effect (defined as
the reaction of fish to a diver). Although widely recognised, its effects have
yet to be quantified and the extent of taxonomic variation remains to be
determined. We therefore examined relative diver effects on a reef fish
assemblage on the Great Barrier Reef. Using common UVC methods, the recorded
abundance of seven reef fish groups were significantly affected by the ongoing
presence of SCUBA divers. Overall, the diver effect resulted in a 52%
decrease in the mean number of individuals recorded, with declines of up to
70% in individual families. Although the diver effect appears to be a
significant problem, UVCs remain a useful approach for quantifying spatial and
temporal variation in relative fish abundances, especially if using methods that
minimise the exposure of fishes to divers. Fixed distance transects using tapes
or lines deployed by a second diver (or GPS-calibrated timed swims) would appear
to maximise fish counts and minimise diver effects
Spinal trigeminal neurons demonstrate an increase in responses to dural electrical stimulation in the orofacial formalin test
Primary headaches are often associated with pain in the maxillofacial region commonly classified under the term “orofacial pain” (OFP). In turn, long-lasting OFP can trigger and perpetuate headache as an independent entity, which is able to persist after the resolution of the main disorder. A close association between OFP and headache complicates their cause and effect definition and leads to misdiagnosis. The precise mechanisms underlying this phenomenon are poorly understood, partly because of the deficiency of research-related findings. We combined the animal models of OFP and headache—the orofacial formalin test and the model of trigeminovascular nociception—to investigate the neurophysiological mechanisms underlying their comorbidity. In anesthetized rats, the ongoing activity of single convergent neurons in the spinal trigeminal nucleus was recorded in parallel to their responses to the electrical stimulation of the dura mater before and after the injection of formalin into their cutaneous receptive fields. Subcutaneous formalin resulted not only in the biphasic increase in the ongoing activity, but also in an enhancement of neuronal responses to dural electrical stimulation, which had similar time profile. These results demonstrated that under tonic pain in the orofacial region a nociceptive signaling from the dura mater to convergent trigeminal neurons is significantly enhanced apparently because of the development of central sensitization; this may contribute to the comorbidity of OFP and headache
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