5,465 research outputs found
Clinical and pathologic characteristics of T-cell lymphoma with a leukemic phase in a raccoon dog (Nyctereutes Procyonoides)
A 7.5-year-old raccoon dog (Nyctereutes procyonoides) from the Henry Doorly Zoo (Omaha, Nebraska) presented to the veterinary hospital for lethargy and weight loss. On physical examination, splenomegaly and hepatomegaly were noted on palpation and were confirmed by radiographic evaluation. Radiography also demonstrated a mass in the cranial mediastinum. A complete blood cell count revealed marked leukocytosis (115,200 cells/microl), with a predominance of lymphoid cells. The animal was euthanized due to a poor prognosis. Necropsy revealed splenomegaly, hepatomegaly, and a large multiloculated mass in the cranial mediastinum. The histologic and immunohistochemical diagnosis was multicentric T-cell lymphoma with a leukemic phase.published_or_final_versio
A stitch in time: Efficient computation of genomic DNA melting bubbles
Background: It is of biological interest to make genome-wide predictions of
the locations of DNA melting bubbles using statistical mechanics models.
Computationally, this poses the challenge that a generic search through all
combinations of bubble starts and ends is quadratic.
Results: An efficient algorithm is described, which shows that the time
complexity of the task is O(NlogN) rather than quadratic. The algorithm
exploits that bubble lengths may be limited, but without a prior assumption of
a maximal bubble length. No approximations, such as windowing, have been
introduced to reduce the time complexity. More than just finding the bubbles,
the algorithm produces a stitch profile, which is a probabilistic graphical
model of bubbles and helical regions. The algorithm applies a probability peak
finding method based on a hierarchical analysis of the energy barriers in the
Poland-Scheraga model.
Conclusions: Exact and fast computation of genomic stitch profiles is thus
feasible. Sequences of several megabases have been computed, only limited by
computer memory. Possible applications are the genome-wide comparisons of
bubbles with promotors, TSS, viral integration sites, and other melting-related
regions.Comment: 16 pages, 10 figure
SNP analysis of the inter-alpha-trypsin inhibitor family heavy chain-related protein (IHRP) gene by a fluorescence-adapted SSCP method
BACKGROUND: Single-nucleotide polymorphisms (SNPs) are considered to be useful polymorphic markers for genetic studies of polygenic traits. Single-stranded conformational polymorphism (SSCP) analysis has been widely applied to detect SNPs, including point mutations in cancer and congenital diseases. In this study, we describe an application of the fluorescent labeling of PCR fragments using a fluorescent-adapted primer for SSCP analysis as a novel method. METHODS: Single-nucleotide polymorphisms (SNPs) of the inter-alpha-trypsin inhibitor family heavy chain-related protein (IHRP) gene were analyzed using a fluorescence-adapted SSCP method. The method was constructed from two procedures: 1) a fluorescent labeling reaction of PCR fragments using fluorescence-adapted primers in a single tube, and 2) electrophoresis on a non-denaturing polyacrylamide gel. RESULTS: This method was more economical and convenient than the single-stranded conformational polymorphism (SSCP) methods previously reported in the detection of the labeled fragments obtained. In this study, eight SNPs of the IHRP gene were detected by the fluorescence-adapted SSCP. One of the SNPs was a new SNP resulting in an amino acid substitution, while the other SNPs have already been reported in the public databases. Six SNPs of the IHRP were associated with two haplotypes. CONCLUSIONS: The fluorescence-adapted SSCP was useful for detecting and genotyping SNPs
Entanglement of spin waves among four quantum memories
Quantum networks are composed of quantum nodes that interact coherently by
way of quantum channels and open a broad frontier of scientific opportunities.
For example, a quantum network can serve as a `web' for connecting quantum
processors for computation and communication, as well as a `simulator' for
enabling investigations of quantum critical phenomena arising from interactions
among the nodes mediated by the channels. The physical realization of quantum
networks generically requires dynamical systems capable of generating and
storing entangled states among multiple quantum memories, and of efficiently
transferring stored entanglement into quantum channels for distribution across
the network. While such capabilities have been demonstrated for diverse
bipartite systems (i.e., N=2 quantum systems), entangled states with N > 2 have
heretofore not been achieved for quantum interconnects that coherently `clock'
multipartite entanglement stored in quantum memories to quantum channels. Here,
we demonstrate high-fidelity measurement-induced entanglement stored in four
atomic memories; user-controlled, coherent transfer of atomic entanglement to
four photonic quantum channels; and the characterization of the full
quadripartite entanglement by way of quantum uncertainty relations. Our work
thereby provides an important tool for the distribution of multipartite
entanglement across quantum networks.Comment: 4 figure
Chordin Is a Modifier of Tbx1 for the Craniofacial Malformations of 22q11 Deletion Syndrome Phenotypes in Mouse
Point mutations in TBX1 can recapitulate many of the structural defects of 22q11 deletion syndromes (22q11DS), usually associated with a chromosomal deletion at 22q1.2. 22q11DS often includes specific cardiac and pharyngeal organ anomalies, but the presence of characteristic craniofacial defects is highly variable. Even among family members with a single TBX1 point mutation but no cytological deletion, cleft palate and low-set ears may or may not be present. In theory, such differences could depend on an unidentified, second-site lesion that modifies the craniofacial consequences of TBX1 deficiency. We present evidence for such a locus in a mouse model. Null mutations of chordin have been reported to cause severe defects recapitulating 22q11DS, which we show are highly dependent on genetic background. In an inbred strain in which chordin−/− is fully penetrant, we found a closely linked, strong modifier—a mutation in a Tbx1 intron causing severe splicing defects. Without it, lack of chordin results in a low penetrance of mandibular hypoplasia but no cardiac or thoracic organ malformations. This hypomorphic Tbx1 allele per se results in defects resembling 22q11DS but with a low penetrance of hallmark craniofacial malformations, unless chordin is mutant. Thus, chordin is a modifier for the craniofacial anomalies of Tbx1 mutations, demonstrating the existence of a second-site modifier for a specific subset of the phenotypes associated with 22q11DS
M5 spikes and operators in the HVZ membrane theory
In this note we study some aspects of the so-called dual ABJM theory
introduced by Hanany, Vegh & Zaffaroni. We analyze the spectrum of chiral
operators, and compare it with the spectrum of functions on the mesonic moduli
space M=C^2\times C^2/Z_k, finding expected agreement for the coherent branch.
A somewhat mysterious extra branch of dimension N^2 opens up at the orbifold
fixed point. We also study BPS solutions which represent M2/M5 intersections.
The mesonic moduli space suggests that there should be two versions of this
spike: one where the M5 lives in the orbifolded C^2 and another where it lives
in the unorbifolded one. While expectedly the first class turns out to be like
the ABJM spike, the latter class looks like a collection of stacks of M5 branes
with fuzzy S^3 profiles. This shows hints of the appearance of the global SO(4)
at the non-abelian level which is otherwise not present in the bosonic
potential. We also study the matching of SUGRA modes with operators in the
coherent branch of the moduli space. As a byproduct, we present some formulae
for the laplacian in conical CY_4 of the form C^n\times CY_{4-n}.Comment: 22 pages, 1 figure. Published version with corrected typos
Heralded quantum entanglement between two crystals
Quantum networks require the crucial ability to entangle quantum nodes. A
prominent example is the quantum repeater which allows overcoming the distance
barrier of direct transmission of single photons, provided remote quantum
memories can be entangled in a heralded fashion. Here we report the observation
of heralded entanglement between two ensembles of rare-earth-ions doped into
separate crystals. A heralded single photon is sent through a 50/50
beamsplitter, creating a single-photon entangled state delocalized between two
spatial modes. The quantum state of each mode is subsequently mapped onto a
crystal, leading to an entangled state consisting of a single collective
excitation delocalized between two crystals. This entanglement is revealed by
mapping it back to optical modes and by estimating the concurrence of the
retrieved light state. Our results highlight the potential of rare-earth-ions
doped crystals for entangled quantum nodes and bring quantum networks based on
solid-state resources one step closer.Comment: 10 pages, 5 figure
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