142 research outputs found
Gametogenesis in the Pacific Oyster Crassostrea gigas: A Microarrays-Based Analysis Identifies Sex and Stage Specific Genes
Background: The Pacific oyster Crassostrea gigas (Mollusca, Lophotrochozoa) is an alternative and irregular protandrous hermaphrodite: most individuals mature first as males and then change sex several times. Little is known about genetic and phenotypic basis of sex differentiation in oysters, and little more about the molecular pathways regulating reproduction. We have recently developed and validated a microarray containing 31,918 oligomers (Dheilly et al., 2011) representing the oyster transcriptome. The application of this microarray to the study of mollusk gametogenesis should provide a better understanding of the key factors involved in sex differentiation and the regulation of oyster reproduction. Methodology/Principal Findings: Gene expression was studied in gonads of oysters cultured over a yearly reproductive cycle. Principal component analysis and hierarchical clustering showed a significant divergence in gene expression patterns of males and females coinciding with the start of gonial mitosis. ANOVA analysis of the data revealed 2,482 genes differentially expressed during the course of males and/or females gametogenesis. The expression of 434 genes could be localized in either germ cells or somatic cells of the gonad by comparing the transcriptome of female gonads to the transcriptome of stripped oocytes and somatic tissues. Analysis of the annotated genes revealed conserved molecular mechanisms between mollusks and mammals: genes involved in chromatin condensation, DNA replication and repair, mitosis and meiosis regulation, transcription, translation and apoptosis were expressed in both male and female gonads. Most interestingly, early expressed male-specific genes included bindin and a dpy-30 homolog and female-specific genes included foxL2, nanos homolog 3, a pancreatic lipase related protein, cd63 and vitellogenin. Further functional analyses are now required in order to investigate their role in sex differentiation in oysters. Conclusions/Significance: This study allowed us to identify potential markers of early sex differentiation in the oyster C. gigas, an alternative hermaphrodite mollusk. We also provided new highly valuable information on genes specifically expressed by mature spermatozoids and mature oocytes
QUBIC VI: cryogenic half wave plate rotator, design and performances
Inflation Gravity Waves B-Modes polarization detection is the ultimate goal
of modern large angular scale cosmic microwave background (CMB) experiments
around the world. A big effort is undergoing with the deployment of many
ground-based, balloon-borne and satellite experiments using different methods
to separate this faint polarized component from the incoming radiation. One of
the largely used technique is the Stokes Polarimetry that uses a rotating
half-wave plate (HWP) and a linear polarizer to separate and modulate the
polarization components with low residual cross-polarization. This paper
describes the QUBIC Stokes Polarimeter highlighting its design features and its
performances. A common systematic with these devices is the generation of large
spurious signals synchronous with the rotation and proportional to the
emissivity of the optical elements. A key feature of the QUBIC Stokes
Polarimeter is to operate at cryogenic temperature in order to minimize this
unwanted component. Moving efficiently this large optical element at low
temperature constitutes a big engineering challenge in order to reduce friction
power dissipation. Big attention has been given during the designing phase to
minimize the differential thermal contractions between parts. The rotation is
driven by a stepper motor placed outside the cryostat to avoid thermal load
dissipation at cryogenic temperature. The tests and the results presented in
this work show that the QUBIC polarimeter can easily achieve a precision below
0.1{\deg} in positioning simply using the stepper motor precision and the
optical absolute encoder. The rotation induces only few mK of extra power load
on the second cryogenic stage (~ 8 K).Comment: Part of a series of 8 papers on QUBIC to be submitted to a special
issue of JCA
QUBIC instrument for CMB polarization measurements
Measurements of cosmic microwave background (CMB) polarization may reveal the presence of a background of gravitational waves produced during cosmic inflation, providing thus a test of inflationary models. The Q&U Bolometric Interferometer for Cosmology (QUBIC) is an experiment designed to measure the CMB polarization. It is based on the novel concept of bolometric interferometry, which combines the sensitivity of bolometric detectors with the properties of beam synthesis and control of calibration offered by interferometers. To modulate and extract the input polarized signal of the CMB, QUBIC exploits Stokes polarimetry based on a rotating half-wave plate (HWP). In this work, we illustrate the design of the QUBIC instrument, focusing on the polarization modulation system, and we present preliminary results of beam calibrations and the performance of the HWP rotator at 300 K
Status of QUBIC, the Q&U Bolometer for Cosmology
The Q&U Bolometric Interferometer for Cosmology (QUBIC) is a novel kind of
polarimeter optimized for the measurement of the B-mode polarization of the
Cosmic Microwave Back-ground (CMB), which is one of the major challenges of
observational cosmology. The signal is expected to be of the order of a few
tens of nK, prone to instrumental systematic effects and polluted by various
astrophysical foregrounds which can only be controlled through multichroic
observations. QUBIC is designed to address these observational issues with a
novel approach that combines the advantages of interferometry in terms of
control of instrumental systematics with those of bolometric detectors in terms
of wide-band, background-limited sensitivity.Comment: Contribution to the 2022 Cosmology session of the 33rd Rencontres de
Blois. arXiv admin note: substantial text overlap with arXiv:2203.0894
- âŠ