Ovário-histerectomia vídeo-assistida com único portal em cadelas: estudo retrospectivo de 20 casos

O objetivo do presente estudo foi avaliar retrospectivamente as 20 primeiras ovário-histerectomias vídeo-assistidas com único portal (SPVA-OSH), realizadas por um cirurgião não proficiente nessa técnica. Vinte cadelas foram submetidas à laparoscopia para SPVA-OSH, com o auxílio de um endoscópio de 10mm, com canal de trabalho de 5mm, inserido por um trocarte, posicionado na região pré-púbica, e coagulação bipolar. O tempo cirúrgico médio foi de 22,95±5,17 minutos. Os cinco primeiros procedimentos consumiram mais tempo cirúrgico do que os demais. A abordagem aos pedículos ovarianos direito e esquerdo foram as etapas que apresentaram maior tempo de execução. Houve complicação leve ou moderada em seis (30%) das 20 cadelas operadas. As complicações foram: hemorragia leve ou moderada em um dos pedículos ovarianos em quatro (20%) cadelas e hemorragia grave, devido à punção do baço com agulha de Veress em dois (10%) animais. A SPVA-OSH foi realizada em cadelas por um cirurgião no início da curva de aprendizado, sem complicações maiores.The aim of the present study was to evaluate retrospectively the first 20 cases of single-port video-assisted ovariohysterectomy (SPVA-OSH) performed by an inexperienced surgeon. Twenty bitches submitted to SPVA-OSH were assessed. A 10mm operative rigid endoscope with one 5 mm working channel and bipolar coagulation was used. The trocar was positioned in the prepubic area of the abdomen. Surgical time and complication rates were assessed descriptively. Mean surgical time was of 22.95±5.17 minutes. Mild or moderate complications were present in six (30%) out of 20 bitches that were operated. The first five surgical procedures spent more time to be performed than the others. The longest transoperative momentum time of execution was spent in the approach to the left and right ovarian pedicles. The complications were: mild or moderate hemorrhage from one of the ovarian pedicles in four bitches (20%) and severe hemorrhage due to accidental puncture of the spleen with Veress needle in two animals (10%). SPVA-OSH was performed in bitches by an inexperienced surgeon, without severe complications.Universidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Departamento de Clínica e Cirurgia VeterináriaUniversidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Departamento de Clínica e Cirurgia Veterinári

Increased production of 27-hydroxycholesterol in human colorectal cancer advanced stage: Possible contribution to cancer cell survival and infiltration

So far, the investigation in cancer cell lines of the modulation of cancer growth and progression by oxysterols, in particular 27-hydroxycholesterol (27HC), has yielded controversial results. The primary aim of this study was the quantitative evaluation of possible changes in 27HC levels during the different steps of colorectal cancer (CRC) progression in humans. A consistent increase in this oxysterol in CRC mass compared to the tumor-adjacent tissue was indeed observed, but only in advanced stages of progression (TNM stage III), a phase in which cancer has spread to nearby sites. To investigate possible pro-tumor properties of 27HC, its effects were studied in vitro in differentiated CaCo-2 cells. Relatively high concentrations of this oxysterol markedly increased the release of pro-inflammatory interleukins 6 and 8, monocyte chemoattractant protein-1, vascular endothelial growth factor, as well as matrix metalloproteinases 2 and 9. The up-regulation of all these molecules, which are potentially able to favor cancer progression, appeared to be dependent upon a net stimulation of Akt signaling exerted by supra-physiological amounts of 27HC