High cell density cultivation of Escherichia coli K4 in a microfiltration bioreactor: a step towards improvement of chondroitin precursor production

<p>Abstract</p> <p>Background</p> <p>The bacteria <it>Escherichia coli </it>K4 produces a capsular polysaccharide (K4 CPS) whose backbone is similar to the non sulphated chondroitin chain. The chondroitin sulphate is one of the major components of the extra-cellular matrix of the vertebrate connective tissues and a high value molecule, widely employed as active principle in the treatment of osteoarthritis. It is usually obtained by extraction from animal tissues, but the risk of virus contaminations, as well as the scarceness of raw material, makes this productive process unsafe and unable to satisfy the growing market demand. In previous studies a new biotechnological process to produce chondroitin from <it>Escherichia coli </it>K4 capsular polysaccharide was investigated and a 1.4 g·L^-1K4 CPS concentration was reached using fed-batch fermentation techniques. In this work, on the trail of these results, we exploited new fermentation strategies to further improve the capsular polysaccharide production.</p> <p>Results</p> <p>The inhibitory effect of acetate on the bacterial cells growth and K4 CPS production was studied in shake flask conditions, while a new approach, that combined the optimization of the feeding profiles, the improvement of aeration conditions and the use of a microfiltration bioreactor, was investigated in three different types of fermentation processes. High polysaccharide concentrations (4.73 ± 0.2 g·L^-1), with corresponding average yields (0.13 ± 0.006 g_{K4 CPS}·g_cdw^-1), were obtained; the increase of K4 CPS titre, compared to batch and fed-batch results, was of 16-fold and 3.3-fold respectively, while average yield was almost 3.5 and 1.4 fold higher.</p> <p>Conclusion</p> <p>The increase of capsular polysaccharide titre confirmed the validity of the proposed fermentation strategy and opened the way to the use of the microfiltration bioreactor for the biotechnological production of chondroitin.</p

e-Participation: Promoting Dialogue and Deliberation between Institutions and Civil Society

Information and Communication Technologies (ICT) are increasingly becoming more pervasive of peoples lives, both for individual and collective usage. Hence, it becomes tempting to develop electronic tools that can constitute alternatives to enhance citizenship, in particular tools that can be used in the context of societal debates of public policies. This report explores the conditions to deploying electronic based public participation methodologies and online ICT based participatory processes within public policy processes. It reflects on the challenges, promises and motivations of using ICT to promote dialogue and deliberation among institutions and the civil society. It consists in part of a revision of the techniques and tools commonly used in electronic public participation processes, referring to case studies where these techniques and tools were employed. Special attention was given to cases where the outcome from the public participation process supported a decision process. The conditions of deployment of e-participation processes in public policy formulation were framed within the concept of quality, specifically in the concept of ¿fitness for purpose¿. An electronic tool was designed and implemented, not only featuring dialogue components, but also collaborative ones, as response to some of the identified challenges. Based on the review of e-participation tools and the preliminary usage of the tool developed, a protocol for quality assurance of e-participation tools is offered.JRC.G.9-Econometrics and statistical support to antifrau

JRC Thinkers ‘N’ Tinkers Makerspace - Concept Note

In this concept note, we make the case for creating an in-house makerspace oriented towards the engagement of citizens in technoscientific innovations: the JRC Thinkers ‘N’ Tinkers Makerspace. The idea behind the makerspace is to have a space located in the premises of the JRC that promotes critical thinking and tinkering about technoscientific issues relevant for policy files focusing on their societal implications. We view it as a safe space where we can promote dialogues with civil society through engagements that move beyond discursive methods.JRC.I.1-Modelling, Indicators and Impact Evaluatio

Host defense peptides identified in human apolipoprotein B as natural food bio-preservatives: Evaluation of their biosafety and digestibility

The employment of chemical agents in the food industry is raising several concerns by consumers and is leading to an increasing interest in natural food preservatives. Among alternatives, host defense peptides (HDPs) have attracted great interest for their ability to preserve food samples from contamination without altering their quality, taste, and organoleptic properties. Recently, we evaluated the applicability of ApoB-derived peptides as novel food bio-preservatives and demonstrated their ability to prevent chicken meat sample contamination when immobilized on chitosan films. To perform a further step towards the applicability of these peptides in the food field, here we evaluated peptides biosafety and digestibility. To do this, we used a multidisciplinary approach including the evaluation of the peptides' toxicity and antimicrobial activity, the analysis of resistance phenotype development, an in silico prediction of the peptides' susceptibility to proteases and the evaluation of the peptides' stability in simulated gastric and intestinal fluids. ApoB-derived peptides were found to be nontoxic when tested on human gastric carcinoma cells SNU-1 and on human colon-rectal adenocarcinoma cells HT-29, and not to induce resistance phenotype in Salmonella strains. Bioinformatic analyses showed that the peptides are susceptible to several proteases, as also confirmed by experiments in simulated gastric and intestinal fluids. Altogether these findings open interesting perspectives to the future applicability of ApoB-derived peptides as novel food biopreservatives

Futures of Work: Perspectives from the Maker Movement

The work presented in this report attempts to explore other realms about the future(s) of work beyond the strongly driven narrative of digital transformation. We have addressed one particular grassroots community, the Maker Movement, which is de facto enabling new models of education, collaborative work, and manufacture. Movements like the Maker Movement can be inspirational of policy making in areas of great complexity and uncertainties as work, employment, jobs are. We suggest that debates about futures of work need to mobilise the imagination, insights and expectations of wide ranges of society. Policy making should be nurturing necessary studies, experiments and conversations until some resilient ideas are found.JRC.I.2-Foresight, Behavioural Insights and Design for Polic

Heterogeneous Organizational Arrangements in Agrifood Chains: A Governance Value Analysis Perspective on the Sheep and Goat Meat Sector of Italy

In the Italian agrifood sector, one observes heterogeneity in the types of quality certification processes. This heterogeneity cannot be explained by standard governance theories like transaction costs economics (TCE). We use the governance value analysis (GVA) perspective that synthesizes TCE and a resources-based view (RBV), to suggest that the observed heterogeneity in organizational forms is a result of heterogeneous differentiating strategies that farms have pursued in the face of competitive pricing pressures. To empirically test GVA, data are obtained using a survey methodology on lamb meat produced by local farms in the Abruzzo region of Italy, challenged by price-costs squeeze. Our empirical test evidences the relevance of the adopted approach, enlightening different organizational arrangements, strictly linked to both the strategic positioning and to the farms’ resources and core competencies

Cardiac biomarkers in clinical practice of dog and cat - a review

A biomarker is an indicator of a normal or pathologic process, or a pharmacologic response to a therapeutic intervention. Nowadays, in veteriary cardiology, the most used biomarkers for assessing the pathological status of the cardio-vascular system, are B-type natriuretic peptide and cardiac troponins. These biomarkers have been very well studied in cardiac pathology in order to assess their diagnostic potential. The aim of the present paper was to discuss the structure, metabolism, function, applicability, reference values and variations in different diseases and to review some practical aspects of the two cardiac biomarkers, used nowadays in small animal cardiology

Quantum phase transitions in Bose–Einstein condensates from a Bethe ansatz perspective

We investigate two solvable models for Bose–Einstein condensates and extract physical information by studying the structure of the solutions of their Bethe ansatz equations. A careful observation of these solutions for the ground state of both models, as we vary some parameters of the Hamiltonian, suggests a connection between the behavior of the roots of the Bethe ansatz equations and the physical behavior of the models. Then, by the use of standard techniques for approaching quantum phase transition – gap, entanglement and fidelity – we find that the change in the scenery in the roots of the Bethe ansatz equations is directly related to a quantum phase transition, thus providing an alternative method for its detection

Training on Citizen Engagement in Policy-relevant Science,Technology and Innovation

Creating the conditions for genuine engagement of citizens and other societal actors in matters of their concern where science and technology are relevant is an issue of increasing political attention; not least because the diffusion of “low cost” and “low tech” media through which citizens can, like never before, express opinions and concerns calls for institutional reflexivity. Public engagement is one of the pillars of the RRI lemma, together with Ethics. This training course responds to a concrete lack of genuine and legitimate places whereby institutions can explore insights, expectations and imaginaries of citizens in matters of concern to all. This training is concerned with the science for policy realm where engagement of citizens is needed and relevant to ensure quality of policy formulation processes in situations described in the framework of “post-normal science” where “facts uncertain, values in dispute, stakes high and decisions urgent". This training will look into participatory approaches to discuss science and technology developments as well as discuss what makes trustful relationships between the scientific community and the public trustworthy.JRC.I.2-Foresight, Behavioural Insights and Design for Polic