Custo de produção de trigo e de aveia: estimativa para a safra 2004.

bitstream/CNPT-2010/40482/1/p-co117.pd

Constrained Allocation Flux Balance Analysis

New experimental results on bacterial growth inspire a novel top-down approach to study cell metabolism, combining mass balance and proteomic constraints to extend and complement Flux Balance Analysis. We introduce here Constrained Allocation Flux Balance Analysis, CAFBA, in which the biosynthetic costs associated to growth are accounted for in an effective way through a single additional genome-wide constraint. Its roots lie in the experimentally observed pattern of proteome allocation for metabolic functions, allowing to bridge regulation and metabolism in a transparent way under the principle of growth-rate maximization. We provide a simple method to solve CAFBA efficiently and propose an "ensemble averaging" procedure to account for unknown protein costs. Applying this approach to modeling E. coli metabolism, we find that, as the growth rate increases, CAFBA solutions cross over from respiratory, growth-yield maximizing states (preferred at slow growth) to fermentative states with carbon overflow (preferred at fast growth). In addition, CAFBA allows for quantitatively accurate predictions on the rate of acetate excretion and growth yield based on only 3 parameters determined by empirical growth laws.Comment: 21 pages, 6 figures (main) + 33 pages, various figures and tables (supporting); for the supplementary MatLab code, see http://tinyurl.com/h763es

Custo de produção de trigo e de aveia: estimativa safra 2003.

bitstream/CNPT-2010/40476/1/p-co111.pd

Ferrugem de soja: estimativas de custo de controle, em Passo Fundo, RS.

bitstream/CNPT-2010/40650/1/p-do32.pd

Qualidade do trigo brasileiro - safra 2002.

bitstream/item/84202/1/CNPT-DOC.-40-03.pd

Qualidade do trigo brasileiro - safra 2003.

Qualidade do trigo brasileiro - safra 2003; Introdução; Organização e método; Estados de abrangência - safra 2003: Bahia, Goiás, Mato Grosso do Sul, Minas Gerais, São Paulo, Paraná, Santa Catarina, Rio Grande do Sul; Glossário; Aspectos relacionados à legislação; Informações sobre cultivares de trigo brasileiro.bitstream/item/84205/1/CNPT-DOC.-45-04.pd

Qualidade comercial do trigo brasileiro: safra 2006.

bitstream/CNPT-2010/40771/1/p-do112.pd

Influence of conformational fluctuations on enzymatic activity: modelling the functional motion of beta-secretase

Considerable insight into the functional activity of proteins and enzymes can be obtained by studying the low-energy conformational distortions that the biopolymer can sustain. We carry out the characterization of these large scale structural changes for a protein of considerable pharmaceutical interest, the human $\beta$-secretase. Starting from the crystallographic structure of the protein, we use the recently introduced beta-Gaussian model to identify, with negligible computational expenditure, the most significant distortion occurring in thermal equilibrium and the associated time scales. The application of this strategy allows to gain considerable insight into the putative functional movements and, furthermore, helps to identify a handful of key regions in the protein which have an important mechanical influence on the enzymatic activity despite being spatially distant from the active site. The results obtained within the Gaussian model are validated through an extensive comparison against an all-atom Molecular Dynamics simulation.Comment: To be published in a special issue of J. Phys.: Cond. Mat. (Bedlewo Workshop