DNA flow cytometry in uveal melanoma. The effect of pre-enucleation irradiation

This find is registered at Portable Antiquities of the Netherlands with number PAN-0006966

High prevalence of long-term cardiovascular, neurological and psychosocial morbidity after treatment for craniopharyngioma.

Item does not contain fulltextINTRODUCTION: The treatment of craniopharyngiomas is associated with long-term morbidity. AIM OF THE STUDY: To assess the long-term functional outcome and mortality rates after treatment for craniopharyngiomas, we audited our data with special focus on cardiovascular, neurological and psychosocial morbidity. PATIENTS AND METHODS: Between 1965 and 2002, 54 consecutive patients underwent surgery for craniopharyngiomas at the Leiden University Medical Centre (LUMC). Fifteen patients (25%) received additional postoperative radiation therapy. The median follow-up period was 10 years (range 1-37 years). RESULTS: Long-term cure rate was 82% and long-term recurrence rate 18%. Visual fields/visual acuity stabilized or improved in 74% of cases. The long-term prevalence rate of hypopituitarism was 89%. In addition, long-term cardiovascular, neurological and psychosocial morbidity rates were high: 22% (risk factors 57%), 49% and 47%, respectively. Female sex was an independent predictor of increased cardiovascular, neurological and psychosocial morbidity (odds ratio 3.78, P = 0.031). Ten patients (18%) died during an 828 person-year follow-up. The actuarial patient survival rates 5, 10 and 20 years after the initial operation were 95, 85 and 85%, respectively. The standardized mortality ratio (SMR) was 2.88 [95% confidence interval (CI) 1.35-4.99]. CONCLUSION: Craniopharyngioma is associated with excessive long-term multisystem morbidity and mortality, especially in female patients, despite a high cure rate. These observations indicate that dedicated long-term follow-up of these patients is required. The purpose of the follow-up should be: first, to look for recurrences and to ensure appropriate endocrine replacement, especially oestrogen replacement in premenopausal females; and second, to achieve intensive control of glucose, lipids, blood pressure and weight, as in any other patient with increased risk for cardiovascular disease

Variability of soybean germplasm in relation to phosphorus uptake and use efficiency Variabilidade em germoplasma de soja para eficiência na absorção e utilização de fósforo

Plant efficiency for phosphorus uptake and utilization may contribute to improve crop yield potential in situations of low P availability. Twenty nine soybean [Glycine max (L.) Merr.] cultivars were evaluated and classified in relation to the response to phosphorus (P) levels in nutrient solution. P uptake and use efficiency were estimated by the variables: shoot and root dry matter (DM) yield, P-concentrations and contents in plant parts and P-efficiency index (EI). The experiment was conducted in a greenhouse, during 1999, at Campinas, State of São Paulo, Brazil. The experimental design consisted of randomized complete blocks, arranged in split-plots, with three replications. The main plots were the P levels in the nutrient solution (64.5; 129; 258 and 516 mmol L-1), and the subplots were the twenty-nine soybean cultivars, grouped according days to maturity. The plants were harvested at flowering, separated in shoots and roots for dry matter determination and chemical analysis. The analyses of variance indicated differences in plant responses to P-levels and cultivars. Multivariate analysis showed high correlation among the variables shoot-DM, total-DM and shoot P-concentration and P-efficiency index (EI). Cultivars were classified in efficient-responsive (ER)-- 'IAC-1', 'IAC-2', 'IAC-4', 'IAC-5', 'IAC-6', 'IAC-9', 'Sta. Rosa' and 'UFV-1'; efficient-non-responsive (ENR) -- 'IAC-7', 'IAC-11', 'IAC-15', 'S. Carlos' and 'Cristalina'; inefficient-responsive (IR) -- 'IAC-8', 'IAC-10', 'IAC-14', 'Bossier' and 'Foscarin'; and inefficient-non-responsive (INR) -- 'IAC-12', 'IAC-13', 'IAC-16', 'IAC-17', 'IAC-18', 'IAC-19', 'IAC-20', 'IAC-22', 'Paraná', 'IAS-5' and 'BR-4'. The efficient-responsive soybean cultivars showed the highest values for shoot and total DM and EI, and the lowest shoot P-concentrations.<br>A eficiência da planta na absorção e utilização de fósforo em situação de baixa disponibilidade do nutriente pode contribuir para aumentar o potencial produtivo da cultura. Vinte e nove cultivares de soja [Glycine max (L.) Merr.] foram avaliados e classificados quanto à resposta a concentrações de fósforo (P) em solução nutritiva. Determinaram-se as variáveis relacionadas com a eficiência na absorção e utilização de P: produção de matéria seca (MS) da parte aérea e das raízes, teores de P nas partes das plantas e os índices de eficiência de utilização de P (IE). O experimento foi instalado em casa de vegetação em Campinas, em 1999. Foi utilizado o delineamento em blocos ao acaso, em parcelas subdivididas com três repetições. As parcelas principais constituíram-se das concentrações de P na solução nutritiva (64,5; 129; 258 e 516 mmol L-1) e as subparcelas, dos vinte e nove cultivares de soja, agrupados de acordo com o ciclo. As plantas foram colhidas no florescimento, separadas em parte aérea e raízes para determinação da matéria seca e análise química de nutrientes. A análise de variância indicou diferenças nas respostas das plantas para concentrações de P e cultivares. A análise multivariada mostrou alta correlação entre a MS de parte aérea e MS-total com os teores de P na parte aérea e índices de eficiência de utilização de P (IE). Os cultivares foram classificados em eficientes e responsivos (ER) -- 'IAC-1', 'IAC-2', 'IAC-4', 'IAC-5', 'IAC-6', 'IAC-9', 'Sta. Rosa' e 'UFV-1'; eficientes não responsivos (ENR) -- 'IAC-7', 'IAC-11', 'IAC-15', 'S. Carlos' e 'Cristalina'; ineficientes responsivos (IR) -- 'IAC-8', 'IAC-10', 'IAC-14', 'Bossier' e 'Foscarin'; e ineficientes não responsivos (INR) -- 'IAC-12', 'IAC-13', 'IAC-16', 'IAC-17', 'IAC-18', 'IAC-19', 'IAC-20', 'IAC-22', 'Paraná', 'IAS-5' e 'BR-4'. Os cultivares eficientes e responsivos mostraram os maiores valores para MS de parte aérea, MS total e IE, e os menores teores de P na parte aérea

The genetic and ecophysiological diversity of Microcystis

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/171576/1/emi15615.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/171576/2/emi15615-sup-0002-FigureS1.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/171576/3/emi15615_am.pd