Biological activity of Citrus spp. metabolites on Ceratitis capitata (Wiedemann).

The Mediterranean fruit fly (medfly), Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), is one of the most injurious pest at global level. During the last years, several electrophysiological and behavioural studies have been carried out in order to investigate plant volatile compound-insect interactions with the aim to use this knowledge in sustainable control techniques. It has been observed that lemons are not attacked by medfly, probably because of the peel oil, that is toxic to other fruit flies. In the present paper electrophysiological recordings were conducted to evaluate the insect sensitivity to peel extract and peel oil of two Sicilian cultivars (Interdonato and Lunario) of Citrus x limon (L.) Burm.f. on C. capitata females. Behavioural bioassays were also performed to show their possible biological activity (repellent, antioviposition, insecticidal). C. limon peel extracts in different solvents (petroleum ether, dichloromethane and methanol) were investigated at various concentrations using a single cell recording technique (stimulation of tarsal taste chemosensilla). Different tarsal taste cell responses to the two cultivars were recorded. The higher sensitivity was evoked by C. limon Interdonato, particularly to the methanol extract, which elicited significant increases in the spike frequency at increasing concentrations. The peel oil of the same cultivars as well as that ones of other two C. limon varieties (Monachello and Femminello) have been tested by EAG techniques. The EAG data showed a high sensitivity (about -8.0/8.5mV) of the medfly antennae to the oils of Citrus spp. and a clear dose-response relationship. Responses of adult females (virgin and mated) to Citrus spp. peel extract were quantified in a double-choice test using yellow spheres (diameter 7.0cm) housed in field cages. Preliminary tests conducted on three extracts of C. limon Interdonato and Lunario have provided interesting results. It was recorded a general decrease of the oviposition on treated spheres compared to control and in the case of the cultivar Lunario, a mortality of insects

Electrophysiological and Behavioral Responses of Theocolax elegans (Westwood) (Hymenoptera: Pteromalidae) to Cereal Grain Volatiles

Volatiles emitted by the host's food would be the first signals used by parasitoids in the host location process and are thought to play an important role in host habitat location. In this study, the olfactory responses of Theocolax elegans (Westwood), a Pteromalid wasp that parasitizes immature stages of stored-product insect pests developing inside cereal or leguminous grains, to volatiles emitted by healthy wheat grains, their hexane extracts, and different doses of three individual compounds previously identified in cereal grain odors were investigated in Y-tube olfactometer and Petri dish arena behavioral bioassays and electroantennogram recordings. In Y-tube olfactometer bioassays, odors from healthy wheat grains and their hexane extracts were attractive to both sexes of T. elegans. Moreover, hexane extracts elicited arresting effects in Petri dish arena. The three synthetic compounds valeraldehyde, maltol, and vanillin elicited dose-dependent responses in both male and female adult wasps confirming the capability of the peripheral olfactory systems to perceive cereal volatiles. In behavioral bioassays, different doses of vanillin were significantly attractive to both sexes

First report of Leptopilina japonica in Europe

5openInternationalItalian coauthor/editorDrosophila suzukii (Matsumura; Diptera: Drosophilidae) is a key pest of sweet cherry and small fruits worldwide. Biological control remains unutilized in the framework of D. suzukii management. Nonetheless, natural enemies may play an important role in regulating this pest. We report for the first time the presence of Leptopilina japonica Novković and Kimura (Hymenoptera: Figitidae) in Europe. Two specimens emerged from ripened fruits and one was collected after direct observation on a cherry tree in June 2019. They showed the distinctive morphological traits already described and shared more than 99% sequence similarity with specimens of L. japonica collected in Asia. This first finding was confirmed by a wider survey carried out in 2020; L. japonica emerged from cherry fruit samples collected in five other sites across the Trentino region, suggesting that L. japonica has already colonized a wide area. Detection of this Asian species is relevant to the future direction in managing D. suzukii, both in Europe and North America. In fact, L. japonica showed similarity with Ganaspis brasiliensis (Ihering) (Hymenoptera: Figitidae), the most promising candidate for the classical biological control, in terms of developmental time, egg maturation, host age preference and lifetime fecundityopenPuppato, S.; Grassi, A.; Pedrazzoli, F.; De Cristofaro, A.; Ioriatti, C.Puppato, S.; Grassi, A.; Pedrazzoli, F.; De Cristofaro, A.; Ioriatti, C

Mating behaviour and dual mode communication of Pear Psylla CACOPSYLLA PYRI

Cacopsylla pyri (L.) (Hemiptera, Psyllidae) is one of the most important pests of European pear, and its management generally depends on the use of chemical insecticides, but C. pyri outbreaks are sometime observed. Ecological control strategies should be desirable and the knowledge of mating behavior is crucial to develop new ones. A multi-approaches research aimed to acquire knowledges about C. pyri mate finding. Electroantennographic (EAG) analyses and olfactometric bioassays were used to evaluate the activity of intraspecific semiochemicals on C. pyri. The EAG amplitudes revealed that volatile compounds, present in female cuticular extracts, elicited dose-dependent responses in males, indicating that these compounds were able to stimulate the male olfactory system. In behavioral bioassays, living females and female cuticular extracts, attracted summerform males in a highly significant manner. Gas chromatography-mass spectrometry revealed that 13-methylheptacosane, 11,13-dimethylheptacosane, 2-methylheptacosane and 3-methylheptacosane were found in larger amounts in female extracts than in male ones, which suggests their role in male attraction. In addition, a laser vibrometer device was used to detect a male-female substrate-born vibrations pattern during pre-copulatory period. The female vibrational signal was recorded as mp3 and conveyed, in loop using a minishaker, on pear shoots with C. pyri virgin pairs to interfere with the mating by masking the natural communications

Selection of lactic acid bacteria species and strains for efficient trapping of Drosophila suzukii

Monitoring of Drosophila suzukii is based on the use of effective traps and baits. The current baits are insufficient to provide efficient monitoring. The use of bacteria as bio-catalyzers to produce bioactive volatiles may improve flies’ attraction. Thus, we conducted this work to improve Droskidrink® bait’s attractiveness using lactic acid bacteria. (2) Different baits that were based on the use of Droskidrink® were assessed for flies’ attraction in a Droso-Trap® in a vineyard. Oenococcus oeni, Pediococcus spp., and Lactobacillus spp. were used. The performance of the most attractive species, O. oeni, inoculated into Droskidrink® was assessed in laboratory tests. The responses of female flies to volatiles produced by Droskidrink® with O. oeni strains were recorded by electroantennography. (3) Preliminary field assessment of baits recorded O. oeni as the most attractive species. Three strain groups showed adaptation to test conditions. Volatiles extracted by the headspace of baits inoculated with O. oeni, elicited electroantennographic responses from fly antennae. (4) Droskidrink® inoculated with O. oeni is a highly attractive bait for monitoring. These findings will be useful for improving the attractiveness of D. suzukii commercial baits based on the utilization of LAB volatiles in a strain-dependent manner

Dataset of pahs determined in home-made honey samples collected in central Italy by means of dllme-gc-ms and cluster analysis for studying the source apportionment

This paper would like to show all the data related to an intensive field campaign focused on the characterization of the Polyaromatic Hydrocarbons (PAHs) composition profile in almost 60 honey samples collected in Central Italy. The analytical data here reported are the base for a study aimed to identify the pollution sources in a region. 22 PAHs were analyzed by means of ultrasound-vortex-assisted dispersive liquid-liquid micro-extraction (DLLME) procedure followed by a triple quadrupole gas chromatograph/mass spectrometer (GC-MS). A chemometrics approach has been carried out for evaluating all the data: in particular, principal component analysis and cluster analysis has been used both for the identification of the main natural/anthropogenic pollutants affecting a site and for evaluating the air quality

Preoperative Multiparametric Ultrasound and Fine Needle Aspiration Cytology evaluation of parotid gland tumors : which is the best technique?

Publisher Copyright: © 2021 Societatea Romana de Ultrasonografie in Medicina si Biologie. All rights reserved.AIMS: To evaluate the pre-surgical diagnostic value of Multiparametric Ultrasound (MPUS) and Fine Needle Aspiration Cytology (FNAC) in differentiating parotid gland tumors, comparing the results with histology. MATERIALS AND METHODS: The study enrolled 84 patients with parotid gland lesions surgically treated in a single tertiary center and evaluated by MPUS. Each patient underwent FNAC. Histological examination was considered the gold standard. RESULTS: Histology identified 62 benign tumors and 22 malignancies. In the differential diagnosis between malignant and benign lesions, B-mode Ultrasound (US), Color-Doppler US, Contrast-Enhanced US (CEUS), Elastography (USE) and FNAC showed the following values of sensitivity: 82%, 81%, 86%, 77%, 73% respectively; specificity: 97%, 61%, 95%, 71%, 97% respectively; PPV: 90%, 43%, 86%, 50%, 89% respectively; NPV: 93%, 90%, 95%, 88%, 91% respectively; and accuracy: 89%, 71%, 90%, 78%, 84% re-spectively. CONCLUSIONS: CEUS proved to be a valid and accurate method for identifying malignant tumors of parotid gland; the combination of B-mode US with CEUS showed similar diagnostic accuracy, but better sensitivity than CEUS taken alone. USE did not improve the diagnostic performance of the B-mode US, alone or in association with CEUS; however, it revealed the highest diagnostic accuracy in the differentiation between benign lesions. FNAC demonstrated lower values in comparison with CEUS and with USE. Therefore, according to our study, MPUS could be proposed as a valid alternative to FNAC.publishersversionPeer reviewe

ORal anticoagulants In fraGile patients with percutAneous endoscopic gastrostoMy and atrIal fibrillation: the (ORIGAMI) study

BACKGROUND: Randomized trials support the safety and efficacy of direct oral anticoagulants (DOACs) versus vitamin K antagonists (VKAs) in patients with nonvalvular atrial fibrillation, leading to increased use of these compounds. Crushed forms of DOACs have been shown to be reliable, but evidence supporting percutaneous endoscopic gastrostomy (PEG) delivery is lacking. PEG is a long-term option for enteral food and drug delivery in patients unable to maintain oral intake, bypassing the risks and disadvantages of parenteral nutrition.AIMS: The ORal anticoagulants In fraGile patients with percutAneous endoscopic gastrostoMy and atrIal fibrillation (ORIGAMI) study investigates the safety and efficacy of Edoxaban administered via PEG in patients with atrial fibrillation and a clinical indication for a long-term anticoagulation.DESIGN: In this prospective, single-centre observational study, 12 PEG-treated patients with indication to anticoagulation will receive edoxaban via PEG and will be followed up to 6 months. Plasma antifactor Xa activity and edoxaban concentrations will be assessed. Thromboembolic (ischaemic stroke, systemic embolism, venous thromboembolism) and bleeding events (Bleeding Academic Research Consortium and Thrombolysis in Myocardial Infarction) will be recorded at 1 and 6 months.PRELIMINARY CASES: A retrospective analysis of five atrial fibrillation cases undergoing PEG implantation at our Institution who received edoxaban via PEG showed plasma anti-FXa levels at a steady state of 146 ± 15 ng/ml, without major adverse event at a mean follow-up of 6 months.CONCLUSION: ORIGAMI prospectively investigates PEG-administration of edoxaban in PEG-treated patients requiring long-term anticoagulation. Our preliminary retrospective data support this route of DOAC administration. CLINICALTRIALS.GOV IDENTIFIER: NCT04271293

Have There Been any Changes in the Epidemiology and Etiology of Maxillofacial Trauma During the COVID-19 Pandemic? An Italian Multicenter Study

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has had a significant impact on people's behavior. The aim of this study has been to evaluate how the SARS-CoV-2 pandemic has impacted the incidence and the features of maxillofacial fractures presented at 6 Italian tertiary centers. Clinical records of all the patients diagnosed for facial fractures between February 23 and May 23, 2019 and 2020 were retrospectively reviewed. Any differences in patient number and characteristics and fracture etiology and site between the 2 groups were then statistically analyzed.There has been a 69.1% decrease in the number of incoming patients during the pandemic. The number of foreign patients has decreased significantly (23.3% versus 9.6%, P\u200a=\u200a0.011) while the average age has increased (38.6 versus 45.6 years old, P\u200a=\u200a0.01). Specific statistical significant differences for accidental falls (31.8% versus 50.1%, P\u200a=\u200a0.005) and sports injuries (16.9% versus 1.4%, P\u200a<\u200a0.001) were found. Concerning fracture sites, significant differences have been found in relation to nasal (22.5% versus 11.4%, P\u200a=\u200a0.009) and frontal sinus (0.9% versus 4.4%, P\u200a=\u200a0.037) fractures. In conclusion, SARS-CoV-2 pandemic has significantly changed the epidemiology and the etiology of facial traumas

Beyond Paradigms in Cultural Astronomy. Proceedings of the 27th SEAC conference held together with the EAA

Proceedings of the 27th SEAC conference held together with the EAA.-- Editors: A. César González-García, Roslyn M. Frank, Lionel D. Sims, Michael A. Rappenglück, Georg Zotti, Juan A. Belmonte, Ivan Šprajc.Cultural Astronomy is the endeavour to understand the role of the sky in past and present societies, and how these societies incorporated the sky into their culture. This broad ranging discipline is closely related to archaeology when investigating material remains of the past. Cultural Astronomy also explores the role of the heavens from the perspectives of the anthropological sciences. In recent decades the discipline has been concerned with methodological and theoretical issues. This volume offers chapters based on presentations at the 27th SEAC meeting held in Bern (2019). These chapters provide a vivid image of front-line research in diverse areas, from Roman light and shadow effects to highlight power, to Maya city organization, Etruscan temple orientation or the ontology of the sky.Peer reviewe