Sheets of vertically aligned BaTiO₃ nanotubes reduce cell proliferation but not viability of NIH-3T3 cells

All biomaterials initiate a tissue response when implanted in living tissues. Ultimately this reaction causes fibrous encapsulation and hence isolation of the material, leading to failure of the intended therapeutic effect of the implant. There has been extensive bioengineering research aimed at overcoming or delaying the onset of encapsulation. Nanotechnology has the potential to address this problem by virtue of the ability of some nanomaterials to modulate interactions with cells, thereby inducing specific biological responses to implanted foreign materials. To this effect in the present study, we have characterised the growth of fibroblasts on nano-structured sheets constituted by BaTiO3, a material extensively used in biomedical applications. We found that sheets of vertically aligned BaTiO3 nanotubes inhibit cell cycle progression - without impairing cell viability - of NIH-3T3 fibroblast cells. We postulate that the 3D organization of the material surface acts by increasing the availability of adhesion sites, promoting cell attachment and inhibition of cell proliferation. This finding could be of relevance for biomedical applications designed to prevent or minimize fibrous encasement by uncontrolled proliferation of fibroblastic cells with loss of material-tissue interface underpinning long-term function of implants

The Golden Beauty: Brain Response to Classical and Renaissance Sculptures

Is there an objective, biological basis for the experience of beauty in art? Or is aesthetic experience entirely subjective? Using fMRI technique, we addressed this question by presenting viewers, naïve to art criticism, with images of masterpieces of Classical and Renaissance sculpture. Employing proportion as the independent variable, we produced two sets of stimuli: one composed of images of original sculptures; the other of a modified version of the same images. The stimuli were presented in three conditions: observation, aesthetic judgment, and proportion judgment. In the observation condition, the viewers were required to observe the images with the same mind-set as if they were in a museum. In the other two conditions they were required to give an aesthetic or proportion judgment on the same images. Two types of analyses were carried out: one which contrasted brain response to the canonical and the modified sculptures, and one which contrasted beautiful vs. ugly sculptures as judged by each volunteer. The most striking result was that the observation of original sculptures, relative to the modified ones, produced activation of the right insula as well as of some lateral and medial cortical areas (lateral occipital gyrus, precuneus and prefrontal areas). The activation of the insula was particularly strong during the observation condition. Most interestingly, when volunteers were required to give an overt aesthetic judgment, the images judged as beautiful selectively activated the right amygdala, relative to those judged as ugly. We conclude that, in observers naïve to art criticism, the sense of beauty is mediated by two non-mutually exclusive processes: one based on a joint activation of sets of cortical neurons, triggered by parameters intrinsic to the stimuli, and the insula (objective beauty); the other based on the activation of the amygdala, driven by one's own emotional experiences (subjective beauty)

Massively Parallel Sequencing and Analysis of the Necator americanus Transcriptome

The blood-feeding hookworm Necator americanus infects hundreds of millions of people. To elucidate fundamental molecular biological aspects of this hookworm, the transcriptome of adult Necator americanus was studied using next-generation sequencing and in silico analyses. Contigs (n = 19,997) were assembled from the sequence data; 6,771 of them had known orthologues in the free-living nematode Caenorhabditis elegans, and most encoded proteins with WD40 repeats (10.6%), proteinase inhibitors (7.8%) or calcium-binding EF-hand proteins (6.7%). Bioinformatic analyses inferred that C. elegans homologues are involved mainly in biological pathways linked to ribosome biogenesis (70%), oxidative phosphorylation (63%) and/or proteases (60%). Comparative analyses of the transcriptomes of N. americanus and the canine hookworm, Ancylostoma caninum, revealed qualitative and quantitative differences. Essential molecules were predicted using a combination of orthology mapping and functional data available for C. elegans. Further analyses allowed the prioritization of 18 predicted drug targets which did not have human homologues. These candidate targets were inferred to be linked to mitochondrial metabolism or amino acid synthesis. This investigation provides detailed insights into the transcriptome of the adult stage of N. americanus

Ethnobotanical knowledge of the Istro-Romanians of Zejane in Croatiǎ

Abstract An ethno-pharmacognostic survey was carried out in one of the smallest ethnic and linguistic groups in Europe: the Istro-Romanians of the village of Zejane (in Croatia), whicȟ has a population of approximately 140 persons, mainly elderly. Using an intensive field participant observation methodology, we recorded about 60 remedies of the local folk pharmacopoeia, and mainly derived from plants. Among them, the uncommon traditions to use homemade vinegar from wild apple (Malus sylvestris) and Cornelian cherries (Cornus mas) for diverse medical purposes, and houseleek (Sempervivum tectorum) against ear pains have been briefly discussed.

Motor control in action: using dance to explore the intricate choreography between action perception and production in the human brain

When experienced dancers watch other dancers perform, they perceive the movement in a quantifiably different manner than nondancers. Is this simply a matter of dancers paying more attention and having greater interest in watching dance, or do quantifiable differences exist within the brains of skilled dancers compared to nondancers related to years of physical practice? Previous neurophysiological research offers insight into this question through the discovery of specialized cells in the monkey brain that are active in a similar manner when monkeys perform or observe the same movement. This discovery of so-called mirror neurons established the idea of a close correspondence between action perception and production. Since this discovery, myriad studies have focused on the relationship between action production and perception in the human brain by studying the execution and observation of simple finger or hand movements. Work with dancers, however, extends such investigations to the full-body domain and helps to uncover how individual experience shapes the links between watching and performing actions. Much of this research uses neuroscientific methods to advance understanding of not only the cerebral phenomena associated with complex action learning and observation but also the neural underpinnings of aesthetic appreciation when watching dance. The results of this work are starting to inform and mutually benefit both the scientific and artistic communities