Cloning Protocol of Aloe vera as a Study-Case for “Tailor-Made” Biotechnology to Small Farmers

Aloe vera has been used worldwide both for pharmaceutical, food, and cosmetic industries due to the plethora of biological activities of some of its metabolites. A study case is reported focusing on the development of a cloning protocol of A. vera to provide propagation material with superior quality to the private sector in southern Brazil, i.e., A. vera juice industry. Such biotechnological approach afforded ca. 4,300 plantlets from 20 explants, over a 6-month period, overcoming the drawback of the lack of propagation material. Typically, the results have led to the increase of the cultured area and juice production of that species coming true the goal of the partnership between the public and private sectors herein involved. The transference of the resulting technology was successfully performed to the company and a patent covering the biotechnological process has been recently requested to official organisms on behalf of the partners.El Aloe vera ha sido empleado mundialmente en las industrias farmacéutica, alimentaria y cosmética, debido a la variedad de actividades biológicas de sus metabolitos. Un estudio de caso es reportado, enfocado en el desarrollo de un protocolo de clonamiento de A. vera para proveer propagación de material de calidad superior al sector privado en el sur de Brasil, por ejemplo, la industria de jugo de A. Vera. Ya que las plantas obtenidas mediante métodos biotecnológicos ascienden a 4300 plántulas desde 20 explantes, en un período de 6 meses, se hace un problema la inminente falta de material. Típicamente los resultados han conducido al incremento de del área cultivada y de la producción de jugo de las especies con verdaderos objetivos de asociatividad entre los sectores privados y públicos. La transferencia de la tecnología resultante fue exitosamente desempeñada hacia la compañía y una patente que protege el proceso biotecnológico ha sido recientemente solicitada a los organismos de patentamiento.Aloe vera has been used worldwide both for pharmaceutical, food, and cosmetic industries due to the plethora of biological activities of some of its metabolites. A study case is reported focusing on the development of a cloning protocol of A. vera to provide propagation material with superior quality to the private sector in southern Brazil, i.e., A. vera juice industry. Such biotechnological approach afforded ca. 4,300 plantlets from 20 explants, over a 6-month period, overcoming the drawback of the lack of propagation material. Typically, the results have led to the increase of the cultured area and juice production of that species coming true the goal of the partnership between the public and private sectors herein involved. The transference of the resulting technology was successfully performed to the company and a patent covering the biotechnological process has been recently requested to official organisms on behalf of the partners

APP metabolism regulates tau proteostasis in human cerebral cortex neurons.

Accumulation of Aβ peptide fragments of the APP protein and neurofibrillary tangles of the microtubule-associated protein tau are the cellular hallmarks of Alzheimer's disease (AD). To investigate the relationship between APP metabolism and tau protein levels and phosphorylation, we studied human-stem-cell-derived forebrain neurons with genetic forms of AD, all of which increase the release of pathogenic Aβ peptides. We identified marked increases in intracellular tau in genetic forms of AD that either mutated APP or increased its dosage, suggesting that APP metabolism is coupled to changes in tau proteostasis. Manipulating APP metabolism by β-secretase and γ-secretase inhibition, as well as γ-secretase modulation, results in specific increases and decreases in tau protein levels. These data demonstrate that APP metabolism regulates tau proteostasis and suggest that the relationship between APP processing and tau is not mediated solely through extracellular Aβ signaling to neurons.This research was supported by grants from Alzheimer’s Research UK and the Wellcome Trust (to F.J.L.) and core funding to the Gurdon Trust from the Wellcome Trust and Cancer Research UK. N.S. was supported by a Woolf-Fisher Trust (NZ) PhD studentship. H.Z. was supported by the Wolfson Centre at UCL, and the UCLH Dementia BRU provided financial support for the collection of patient materials. F.J.L. is a Wellcome Trust Senior Investigator, K.B. is a Torsten So¨ derberg Academy Professor, and H.Z. is a Wallenberg Academy Fellow.This is the final version. It was first published by Elsevier at http://www.sciencedirect.com/science/article/pii/S2211124715003599

Advances on Network Protocols and Algorithms for Vehicular Ad Hoc Networks

Vehicular Ad Hoc Network (VANET) is an emerging area of wireless ad hoc networks that facilitates ubiquitous connectivity between smart vehicles through Vehicle-to-Vehicle (V2V) or Vehicle-to-Roadside (V2R) and Roadside-to- Vehicle (R2V) communications. This emerging field of technology aims to improve safety of passengers and traffic flow, reduces pollution to the environment and enables in-vehicle entertainment applications. The safety-related applications could reduce accidents by providing drivers with traffic information such as collision avoidances, traffic flow alarms and road surface conditions. Moreover, the passengers could exploit an available infrastructure in order to connect to the internet for infomobility and entertainment applications.Lloret, J.; Ghafoor, KZ.; Rawat, DB.; Xia, F. (2013). Advances on Network Protocols and Algorithms for Vehicular Ad Hoc Networks. Mobile Networks and Applications. 18(6):749-754. doi:10.1007/s11036-013-0490-7S749754186Lloret J, Canovas A, Catalá A, Garcia M (2013) Group-based protocol and mobility model for VANETs to offer internet access. J Netw Comput Appl 36(3):1027–1038. doi: 10.1016/j.jnca.2012.02.009Khokhar RH, Zia T, Ghafoor KZ, Lloret J, Shiraz M (2013) Realistic and efficient radio propagation model for V2X communications. KSII Trans Internet Inform Syst 7(8):1933–1953. doi: 10.3837/tiis.2013.08.011Ghafoor KZ (2013) Routing protocols in vehicular ad hoc networks: survey and research challenges, Netw Protocol Algorithm 5(4). doi: 10.5296/npa.v5i4.4134Ghafoor KZ, Bakar KA, Lloret J, Ke C-H, Lee KC (2013) Intelligent beaconless geographical routing for urban vehicular environments. Wirel Netw 19(3):345–362. doi: 10.1007/s11276-012-0470-zGhafoor KZ, Bakar KA, Lee K, AL-Hashimi H (2010) A novel delay- and reliability- aware inter-vehicle routing protocol. Netw Protocol Algorithms 2(2):66–88. doi: 10.5296/npa.v2i2.427Dias JAFF, Rodrigues JJPC, Isento JN, Pereira PRBA, Lloret J (2011) Performance assessment of fragmentation mechanisms for vehicular delay-tolerant networks. EURASIP J Wirel Commun Netw 2011(195):1–14. doi: 10.1186/1687-1499-2011-195Zhang D, Yang Z, Raychoudhury V, Chen Z, Lloret J (2013) An energy-efficient routing protocol using movement trend in vehicular Ad-hoc networks. Comput J 58(8):938–946. doi: 10.1093/comjnl/bxt028Ghafoor KZ, Lloret J, Bakar KA, Sadiq AS, Mussa SAB (2013) Beaconing approaches in vehicular Ad Hoc networks: a survey. Wirel Pers Commun. doi: 10.1007/s11277-013-1222-9Sadiq AS, Bakar KA, Ghafoor KZ, Lloret J (2013) An intelligent vertical handover scheme for audio and video streaming in heterogeneous vehicular networks. Mobile Netw Appl. doi: 10.1007/s11036-013-0465-8Khamayseh YM (2013) Network size estimation in VANETs. Netw Protocol Algorithm 5(3):136–152. doi: 10.5296/npa.v5i6.3838Rawat DB, Popescu DC, Yan G, Olariu S (2011) Enhancing VANET performance by joint adaptation of transmission power and contention window size. IEEE Trans Parallel Distrib Syst 22(9):1528–1535Yan G, Rawat DB, Bista BB. Provisioning vehicular ad hoc networks with quality of services. Int J Space-Based Situated Comput 2(2):104–111Rawat DB, Bista BB, Yan G, Weigle MC (2011) Securing vehicular ad-hoc networks against malicious drivers: a probabilistic approach, International Conference on Complex, Intelligent, and Software Intensive Systems Pp. 146–151. June 30, 2011Sun W, Xia F, Ma J, Fu T, Sun Y. An optimal ODAM-based broadcast algorithm for vehicular Ad-Hoc Networks. KSII Trans Internet Inform Syst 6(12): 3257–3274Vinel AV, Dudin AN, Andreev SD, Xia F (2010) Performance modeling methodology of emergency dissemination algorithms for vehicular ad-hoc networks, 6th Communication Systems, Networks & Digital Signal Processing (CSNDSP 2010), Pp. 397–400AL-Hashimi HN, Bakar KA, Ghafoor KZ (2010) Inter-domain proxy mobile IPv6 based vehicular network. Netw Protocol Algorithm 2(4):1–15. doi: 10.5296/npa.v2i4.488Ghafoor KZ, Bakar KA, Mohammed MA, Lloret J (2013) Vehicular cloud computing: trends and challenges, in the book “mobile computing over cloud: technologies, services, and applications”. IGI GlobalYan G, Rawat DB, Bista BB (2012) Towards secure vehicular clouds, Sixth International Conference on Complex, Intelligent and Software Intensive Systems (CISIS 2012), Pp. 370–375Fernández H, Rubio L, Reig J, Rodrigo-Peñarrocha VM, Valero A (2013) Path loss modeling for vehicular system performance and communication protocols evaluation. Mobile Netw Appl. doi: 10.1007/s11036-013-0463-xAllouche Y, Segal M (2013) A cluster-based beaconing approach in VANETs: near optimal topology via proximity information. Mobile Netw Appl. doi: 10.1007/s11036-013-0468-5Merah AF, Samarah S, Boukerche A, Mammeri A (2013) A sequential patterns data mining approach towards vehicular route prediction in VANETs. Mobile Netw Appl. doi: 10.1007/s11036-013-0459-6Zhang D, Huang H, Zhou J, Xia F, Chen Z (2013) Detecting hot road mobility of vehicular Ad Hoc Networks. Mobile Netw Appl. doi: 10.1007/s11036-013-0467-6El Ajaltouni H, Boukerche A, Mammeri A (2013) A multichannel QoS MAC with dynamic transmit opportunity for. Mobile Netw Appl. doi: 10.1007/s11036-013-0475-6Reñé S, Esparza O, Alins J, Mata-Díaz J, Muñoz JL (2013) VSPLIT: a cross-layer architecture for V2I TCP services over. Mobile Netw Appl. doi: 10.1007/s11036-013-0473-8Blanco B, Liberal F (2013) Amaia Aguirregoitia, application of cognitive techniques to adaptive routing for VANETs in city environments. Mobile Netw Appl. doi: 10.1007/s11036-013-0466-7Kim J, Krunz M (2013) Spectrum-aware beaconless geographical routing protocol for cognitive radio enabled vehicular networks. Mobile Netw Appl. doi: 10.1007/s11036-013-0476-5Dias JAFF, Rodrigues JJPC, Isento JNG, Niu J (2013) The impact of cooperative nodes on the performance of vehicular delay-tolerant networks. Mobile Netw Appl. doi: 10.1007/s11036-013-0464-9Sadiq AS, Bakar KA, Ghafoor KZ, Lloret J, Khokhar R (2013) An intelligent vertical handover scheme for audio and video streaming in heterogeneous vehicular networks. Mobile Netw Appl. doi: 10.1007/s11036-013-0465-8Machado S, Ozón J, González AJ, Ghafoor KZ (2013) Structured peer-to-peer real time video transmission over vehicular Ad Hoc networks. Mobile Netw Appl. doi: 10.1007/s11036-013-0461-zLin C, Wu G, Xia F, Yao L (2013) Enhance the attacking efficiency of the node compromise attack in vehicular Ad-hoc network using connected dominating set. Mobile Netw Appl. doi: 10.1007/s11036-013-0469-

Double-chambered right ventricle in an adult patient diagnosed by transthoracic echocardiography

BACKGROUND: Double-chambered right ventricle is a rare congenital disease frequently misdiagnosed in the adult patient. An anomalous muscle band divides the right ventricle in two cavities causing variable degree of obstruction. Although echocardiography is considered a useful method for the diagnosis of this pathology in children, it has been recognized the transthoracic scanning limitation in adults. CASE PRESENTATION: A 29 year-old patient with double-chambered right ventricle presenting mild exercise intolerance referred for follow up of a known ventricular septal defect in whom a complete diagnosis was obtained based only on transthoracic two dimensional echocardiography without the needing of cardiac catheterization. CONCLUSION: Based on non invasive echocardiographic diagnosis, patient was referred to surgical correction, which was completely successful

A new approach to bad news effects on volatilit y: the multiple-sign-volume sensitive regime EGARCH model (MSV-EGARCH)

In this paper, using daily data for six major international stock market indexes and a modified EGARCH specification, the links between stock market returns, volatility and trading volume are investigated in a new nonlinear conditional variance framework with multiple regimes and volume eff ects. Volatility forecast comparisons, using the Harvey-Newbold test for multiple forecasts encompassing, seem to demonstrate that the MSV- EGARCH complex threshold structure is able to correctly fit GARCH- type dynamics of the series under study and dominates competing standard asymmetric models in several of the considered stock indexes.info:eu-repo/semantics/publishedVersio

Expression-Based In Silico Screening of Candidate Therapeutic Compounds for Lung Adenocarcinoma

BACKGROUND:Lung adenocarcinom (AC) is the most common form of lung cancer. Currently, the number of medical options to deal with lung cancer is very limited. In this study, we aimed to investigate potential therapeutic compounds for lung adenocarcinoma based on integrative analysis. METHODOLOGY/PRINCIPAL FINDINGS:The candidate therapeutic compounds were identified in a two-step process. First, a meta-analysis of two published microarray data was conducted to obtain a list of 343 differentially expressed genes specific to lung AC. In the next step, expression profiles of these genes were used to query the Connectivity-Map (C-MAP) database to identify a list of compounds whose treatment reverse expression direction in various cancer cells. Several compounds in the categories of HSP90 inhibitor, HDAC inhibitor, PPAR agonist, PI3K inhibitor, passed our screening to be the leading candidates. On top of the list, three HSP90 inhibitors, i.e. 17-AAG (also known as tanespimycin), monorden, and alvespimycin, showed significant negative enrichment scores. Cytotoxicity as well as effects on cell cycle regulation and apoptosis were evaluated experimentally in lung adenocarcinoma cell line (A549 or GLC-82) with or without treatment with 17-AAG. In vitro study demonstrated that 17-AAG alone or in combination with cisplatin (DDP) can significantly inhibit lung adenocarcinoma cell growth by inducing cell cycle arrest and apoptosis. CONCLUSIONS/SIGNIFICANCE:We have used an in silico screening to identify compounds for treating lung cancer. One such compound 17-AAG demonstrated its anti-lung AC activity by inhibiting cell growth and promoting apoptosis and cell cycle arrest

Simulated effect of pneumococcal vaccination in the Netherlands on existing rules constructed in a non-vaccinated cohort predicting sequelae after bacterial meningitis

BACKGROUND: Previously two prediction rules identifying children at risk of hearing loss and academic or behavioral limitations after bacterial meningitis were developed. Streptococcus pneumoniae as causative pathogen was an important risk factor in both. Since 2006 Dutch children receive seven-valent conjugate vaccination against S. pneumoniae. The presumed effect of vaccination was simulated by excluding all children infected by S. pneumoniae with the serotypes included in the vaccine, from both previous collected cohorts (between 1990-1995). METHODS: Children infected by one of the vaccine serotypes were excluded from both original cohorts (hearing loss: 70 of 628 children; academic or behavioral limitations: 26 of 182 children). All identified risk factors were included in multivariate logistic regression models. The discriminative ability of both new models was calculated. RESULTS: The same risk factors as in the original models were significant. The discriminative ability of the original hearing loss model was 0.84 and of the new model 0.87. In the academic or behavioral limitations model it was 0.83 and 0.84 respectively. CONCLUSION: It can be assumed that the prediction rules will also be applicable on a vaccinated population. However, vaccination does not provide 100% coverage and evidence is available that serotype replacement will occur. The impact of vaccination on serotype replacement needs to be investigated, and the prediction rules must be validated externally