Síndrome poliglandular autoimune tipo 1: descrição de caso e revisão da literatura

Autoimmune polyendocrine syndrome type 1 (APECED) is a rare autosomal recessive disorder characterized by autoimmune multiorgan attack. The disease is caused by mutations in the autoimmune regulator gene (AIRE), resulting in defective AIRE protein, which is essential for selftolerance. Clinical manifestations are widely variable. Although the classic triad is composed by mucocutaneous candidiasis, hypoparathyroidism and adrenal failure, many other components may develop. Treatment is based on supplementation of the various deficiencies, and patients require regular follow-up throughout their lifespan. This article describes the case of a patient with the disease, and reviews literature data on the epidemiology, clinical course, immunogenetic aspects, diagnosis and treatment of the syndrome.Síndrome poliglandular autoimune tipo 1 é uma rara desordem autossômica recessiva caracterizada por ataque autoimune a diversos órgãos. A doença é causada por mutações no gene AIRE (autoimmune regulator), resultando em uma proteína AIRE defeituosa, proteína esta essencial para a manutenção da autotolerância. As manifestações clínicas são extremamente variáveis. A tríade clássica é composta por candidíase mucocutânea crônica, hipoparatiroidismo e insuficiência adrenal, porém diversos outros componentes podem estar presentes. A base do tratamento é a reposição das diversas deficiências, e os pacientes devem ser acompanhados por toda a vida. Este artigo descreve o caso de uma paciente com a síndrome e apresenta uma revisão sobre a epidemiologia, quadro clínico, aspectos imunogenéticos, diagnóstico e tratamento da desordem, de acordo com a literatura publicada.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Division of EndocrinologyUNIFESP-EPM Biochemistry DepartmentUNIFESP, EPM, Division of EndocrinologyUNIFESP, EPM Biochemistry DepartmentSciEL

Using static, kinetic and metal mobility procedures to evaluate possibilities of coal waste land disposal at Moatize Mine, Mozambique

Coal mine waste rocks produced during mining and beneficiation can affect the environment due to land degradation and acid mine drainage generation. However, proper characterization and treatment of mineral processing wastes creates options for safe disposal, enhancing the environmental performance. The aim of this study was to use static and kinetic tests to assess properties of coal waste produced at the Moatize Mine in Mozambique, one of the largest "world-class" coal deposits. The study included sampling and characterization of coarse and fine wastes generated during coal beneficiation. Both materials were analyzed in terms of granular properties, static procedures - immediate composition, sulfur forms, mineralogical composition, elemental composition, acid-base balance – and kinetic behavior in humidity cell tests. The conjugation of these techniques indicates that these wastes have a low water acidification potential and low geochemical mobility of toxic elements. Therefore, it was concluded that fine and coarse wastes could be used in land shaping procedures

Progênies híbridas de grama forquilha : avaliações agronômicas

Paspalum notatum Flüggeis one native forage grasses of southern Brazil. Through intraspecific hybridization, is possible generating progenies with variability for agronomic traits.The objective of this work was to evaluate 24 hybrids and select them for the superior agronomic traits, as well analyze correlations between forage traits that can assist in the selection of genotypes.The hybrids were obtained from crosses among sexual and apomictic clones, and were vegetative propagated in a greenhouse until the transplant to the field. The agronomic traits evaluated were: total green mass (TGM), leaf dry mass (LDM), stem dry mass (SDM), inflorescence dry mass (IDM), dead mass (DeM), total dry mass (TDM),growth habit (GH). Correlations between plant diameter (PD), number of tillers (NT), total dry matter (TDM), plant height (PH), total green matter (TGM), leaf dry matter (LDM), stem dry mass (SDM), number of inflorescences (NINF) and inflorescence dry matter (IDM) were performed using the Pearson correlation coefficient. The hybrids had great variability in all agronomic characteristics measured. Based on the three years of evaluation, hybrids KD9, KF1, KF4, and KD5 produced higher total dry mass than othergenotypes. The hybrid KF4 too had greater LDM; KF1 and KF4 were the colder tolerant. These genotypes were selected for evaluations, such as seed production, fertilizer use efficiency, animal performance, and for new crosses. The high correlation of the total dry mass with the other forage components will be useful for indirect select criteria in bahiagrass improving strategies.Paspalum notatumFlügge é uma gramínea forrageira nativa do sul do Brasil. Hibridizações intraespecíficas geram progênies com variabilidade para características agronômicas. O objetivo deste trabalho foi avaliar 24 híbridos e selecioná-los pelas características agronômicas superiores, bem como analisar correlações entre características forrageiras que possam auxiliar na seleção de genótipos. Os híbridos foram obtidos através dos cruzamentos entre clones sexuais e apomíticos, e foram propagados vegetativamente em casa de vegetação até o transplante para o campo. As características agronômicas avaliadas foram: massa verde total (MVT), massa seca das folhas (MSF), massa seca do caule (MSC), massa seca da inflorescência (MSI), material morta (MM), massa seca total (MST), hábito de crescimento (HC). As correlações entre diâmetro da planta (DP), número de perfilhos (NP), massa seca total (MST), altura da planta (Alt), massa verde total (MVT), massa seca das folhas (MSF), massa seca do caule (MSC), número de inflorescências (NINFL) e massa seca das inflorescências (MSINFL) foram realizados utilizando o coeficiente de correlação de Pearson. Os híbridos tiveram grande variabilidade em todas as características medidas. Em três anos de avaliações, os híbridos KD9, KF1, KF4 e KD5 produziram maior MST; KF4 apresentou maior MSF; KF1 e KF4 foram os mais tolerantes ao frio. Esses genótipos foram selecionados para avaliações de produção de sementes, eficiência no uso de fertilizantes, desempenho animal e para novos ciclos de cruzamentos. A alta correlação da massa seca total com outros componentes da forragem será útil para critérios de seleção indireta em estratégias de melhoramento da grama forquilha

Hybrid progenies of Bahiagrass: agronomic evaluation

Paspalum notatum Flügge (bahiagrass) is one of the most common native grasses found in southern Brazil and have become one of the most promising forage species considering their value for beef cattle production in the tropics and subtropics. The breeding program has been possible through intraspecific hybridization among sexual and apomictic clones, generating progenies with variability for agronomic traits. Hybrid materials with superior agronomic performance can be selected, registered and protected to be made available as a commercial cultivar. The objective of this work was to evaluate 24 intraspecific hybrids and select them for the superior agronomic traits, as well analyze correlations between forage traits that can assist in the selection of genotypes. Based on the three years of evaluation, hybrids KD9, KF1, KF4, and KD5 produced higher total dry mass than other genotypes. The hybrid KF4 too had greater leaf dry matter; KF1 and KF4 were the more cold tolerant. These genotypes were selected for new cycles of hybridization and field evaluations, such as seed production, fertilizer use efficiency and animal performance. The high correlation of the total dry mass with the other forage components might be useful for indirect select criteria in bahiagrass improving strategies.Paspalum notatum Flügge is one native forage grasses of southern Brazil. Through intraspecific hybridization, is possible generating progenies with variability for agronomic traits. The objective of this work was to evaluate 24 hybrids and select them for the superior agronomic traits, as well analyze correlations between forage traits that can assist in the selection of genotypes.The hybrids were obtained from crosses among sexual and apomictic clones, and were vegetative propagated in a greenhouse until the transplant to the field. The agronomic traits evaluated were: total green mass (TGM), leaf dry mass (LDM), stem dry mass (SDM), inflorescence dry mass (IDM), dead mass (DeM), total dry mass (TDM), growth habit (GH). Correlations between plant diameter (PD), number of tillers (NT), total dry matter (TDM), plant height (PH), total green matter (TGM), leaf dry matter (LDM), stem dry mass (SDM), number of inflorescences (NINF) and inflorescence dry matter (IDM) were performed using the Pearson correlation coefficient. The hybrids had great variability in all agronomic characteristics measured. Based on the three years of evaluation, hybrids KD9, KF1, KF4, and KD5 produced higher total dry mass than other genotypes. The hybrid KF4 too had greater LDM; KF1 and KF4 were the colder tolerant. These genotypes were selected for evaluations, such as seed production, fertilizer use efficiency, animal performance, and for new crosses. The high correlation of the total dry mass with the other forage components will be useful for indirect select criteria in bahiagrass improving strategies

Tannins possessing bacteriostatic effect impair pseudomonas aeruginosa adhesion and biofilm formation

Plants produce many compounds that are biologically active, either as part of their normal program of growth and development or in response to pathogen attack or stress. Traditionally, Anadenanthera colubrina, Commiphora leptophloeos and Myracrodruon urundeuva have been used by communities in the Brazilian Caatinga to treat several infectious diseases. The ability to impair bacterial adhesion represents an ideal strategy to combat bacterial pathogenesis, because of its importance in the early stages of the infectious process; thus, the search for anti-adherent compounds in plants is a very promising alternative. This study investigated the ability of stem-bark extracts from these three species to control the growth and prevent biofilm formation of Pseudomonas aeruginosa, an important opportunistic pathogen that adheres to surfaces and forms protective biofilms. A kinetic study (0–72 h) demonstrated that the growth of extract-treated bacteria was inhibited up to 9 h after incubation, suggesting a bacteriostatic activity. Transmission electron microscopy and fluorescence microscopy showed both viable and nonviable cells, indicating bacterial membrane damage; crystal violet assay and scanning electron microscopy demonstrated that treatment strongly inhibited biofilm formation during 6 and 24 h and that matrix production remained impaired even after growth was restored, at 24 and 48 h of incubation. Herein, we propose that the identified (condensed and hydrolyzable) tannins are able to inhibit biofilm formation via bacteriostatic properties, damaging the bacterial membrane and hindering matrix production. Our findings demonstrate the importance of this abundant class of Natural Products in higher plants against one of the most challenging issues in the hospital setting: biofilm resilience

Strategies to mitigate the emission of methane in pastures: Enteric methane: A review

The global population reached 7.9 billion in 2021, which represents a 160% increase in the number of people to be fed since 1960. Agricultural systems must sustainably meet food demand for this growing population while minimizing or mitigating potential environmental impacts, which are of growing concern to both consumers and the scientific community. High protein animal products (meat and milk) play a crucial part in human nutrition and pastures represent ~20% of the planet’s surface. Pastoral areas have a great influence on both ecological balance and human subsistence. Ruminant livestock production systems are hotly debated because of the emission of methane, which is produced during enteric fermentation of ingested food within the rumen. Methanogenesis is a naturally occurring process in the digestive system of ruminant animals and ingesting a high-quality diet has been shown to reduce methane production. An additional function of pastoral grasslands is the capacity of the soils to operate as carbon sinks. Well managed pastures absorb carbon from the atmosphere where it can add to soil organic matter directly, through residue decomposition or excrement returns. However, in Brazil and globally, the efficiency of animal productivity tends to be lower in extensively grazed farming systems. Changes to pasture and grazing management in combination with the adoption of technology is necessary to improve the quality of pastures, increase animal productivity, and consequently reduce methane emissions from ruminant livestock. This review will discuss how to improve the conversion efficiency using pasture management to reduce or mitigate enteric methane production