O lúdico na educação infantil: do brincar ao aprender

Increasingly, research shows that the play needs to be passed in early childhood education, there are many possibilities that it can enjoy recreational activities that promote mediation of interactive learning content. Think and reect on this issue is very important to facilitate teachers' actions that stimulate children's learning. The present work has the objective to demonstrate the importance of playfulness in child development, as favorable to their development tool. The methodological instrument is anchored in qualitative research with bibliographic in selecting books and articles about the inuence of playfulness in child development. An investigation of eld observations in the classroom as well as questionnaire and their analysis. The new forms of interactive production are being increasingly used in motor, cognitive, affective and social that promotes and stimulates learnin . Playing distinguishes didactic learning to be developed for age range of early childhood education, which emphasizes and focuses on the space for entertaining. Teaching practice plays a key purpose in teacher education, it is that there is the possibility of making theoretically aware and reective practices . It is an eminently practical lesson that will help make sense of all the theoretical body that studied the formation.Cada vez mais pesquisas mostram que o lúdico precisa ser aproveitado na educação infantil, são muitas as possibilidades em que a mesma pode usufruir de atividades lúdicas que promovam a mediação interativa dos conteúdos de ensino. Pensar e reetir sobre esse tema é muito importante para facilitar as ações docentes que estimulam a aprendizagem da criança. O presente trabalho tem por objetivo geral demonstrar a importância do lúdico no desenvolvimento da criança, como instrumento favorável ao seu desenvolvimento. O instrumento metodológico está ancorado na pesquisa qualitativa com levantamento bibliográfico, em livros e artigos selecionando informações sobre a inuência do lúdico no desenvolvimento da criança. Uma investigação de campo com observações em sala de aula como também questionário e suas respectivas analises. As novas formas de produção interativa estão sendo usadas cada vez mais no desenvolvimento motor, cognitivo, afetivo e social que propicia e estimula a aprendizagem. O brincar distingue a didática de aprendizagem a ser desenvolvido para faixa de idade da educação infantil, o que enfatiza e enfoca o espaço para o lúdico. A prática pedagógica assume um propósito fundamental na formação docente, é nela que há a possibilidade de tornar as práticas teoricamente conscientes e reexivas. É uma aula eminentemente prática, que vai ajudar a dar sentido a todo o corpo teórico que se estudou na formação

Hepatitis C Virus Antigenic Convergence

Vaccine development against hepatitis C virus (HCV) is hindered by poor understanding of factors defining cross-immunoreactivity among heterogeneous epitopes. Using synthetic peptides and mouse immunization as a model, we conducted a quantitative analysis of cross-immunoreactivity among variants of the HCV hypervariable region 1 (HVR1). Analysis of 26,883 immunological reactions among pairs of peptides showed that the distribution of cross-immunoreactivity among HVR1 variants was skewed, with antibodies against a few variants reacting with all tested peptides. The HVR1 cross-immunoreactivity was accurately modeled based on amino acid sequence alone. The tested peptides were mapped in the HVR1 sequence space, which was visualized as a network of 11,319 sequences. The HVR1 variants with a greater network centrality showed a broader cross-immunoreactivity. The entire sequence space is explored by each HCV genotype and subtype. These findings indicate that HVR1 antigenic diversity is extensively convergent and effectively limited, suggesting significant implications for vaccine development

American cutaneous leishmaniasis in dogs from an endemic urban area in Cianorte municipality, Paraná State, Brazil

American cutaneous leishmaniasis (ACL) was investigated in dogs from an urban endemic area in Cianorte, Paraná state, Brazil. Of 169 studied dogs, none presented suspected ACL lesions. Eleven animals (6.6%) had anti-Leishmania braziliensis antibodies (titers > 40) detected by the immunofluorescent antibody test (IFAT) while four (2.4%) showed L. braziliensis-complex DNA by the polymerase chain reaction (PCR). Although no associations were found between IFAT or PCR results and age, sex, origin, free-roaming animals or length of residence at the address, the majority of IFAT- or PCR-positive dogs were from the urban area of the city and were allowed to roam freely beyond their neighborhood. The presence of anti-Leishmania braziliensis antibodies and L. braziliensis-complex DNA in dogs from this urban area near a native-forest park indicates the importance of following up on these dogs to confirm the ACL diagnosis

First characterization of congenital myasthenic syndrome type 5 in North Africa

International audienceBackground: Congenital myasthenic syndromes (CMS) are associated with defects in the structure and the function of neuromuscular junctions. These rare disorders can result from mutations in the collagenic tail of endplate acetylcholinesterase (COLQ) essentially associated with autosomal recessive inheritance. With the lowered cost of genetic testing and increased access to next-generation sequencing, many mutations have been reported to date.Methods and results: In this study we identified the first COLQ homozygous mutation c.1193T>A in the North African population. This study outlines the genetic and phenotypic features of a CMS patient in a Moroccan family. It also describes a novel COLQ missense mutation associated with CMS-5.Conclusion: COLQ mutations are probably underdiagnosed in these North African populations, this is an issue as CMS-5 may be treated with ephedrine, and albuterol. Indeed, patients can seriously benefit and even recover after the treatment that should be planned according to genetic tests and clinical findings

Identification of novel mutations by targeted NGS in Moroccan families clinically diagnosed with a neuromuscular disorder

International audienceBackground and aims: The identification of underlying genes of genetic conditions has expanded greatly in the past decades, which has broadened the field of genes responsible for inherited neuromuscular diseases. We aimed to investigate mutations associated with neuromuscular disorders phenotypes in 2 Moroccan families.Material and methods: Next-generation sequencing combined with Sanger sequencing could assist with under-standing the hereditary variety and underlying disease mechanisms in these disorders. Results: Two novel homozygous mutations were described in this study. The SIL1 mutation is the first identified in the Moroccan population, the mutation was identified as the main cause of Marinesco-Sjogren syndrome in one patient. While the second mutation identified in the fatty acid 2-hydroxylase gene (FA2H) was associated with the Spastic paraplegia 35 in another patient, both transmitted in an autosomal recessive pattern.Discussion and conclusions: These conditions are extremely rare in the North African population and may be underdiagnosed due to overlapping clinical characteristics and heterogeneity of these diseases. We have reported in this study mutations associated with the diseases found in the patients. In addition, we have narrowed the phenotypic spectrum, as well as the diagnostic orientation of patients with neuromuscular disorders, who might have very similar symptoms to other disease groups