Effects of reduced glutathione and catalase on the kinematics and membrane functionality of sperm during liquid storage of ram semen

AbstractThe objective of this study was to evaluate the effects of reduced glutathione (GSH) and catalase (CAT) supplementation on the kinematics and membrane functionality of sperm during the liquid storage of ram semen, cooled at 5°C, for up to 24h. Semen samples from four rams were pooled, diluted with Tris-egg yolk extender without antioxidants (control) or supplemented with either CAT (100, 200, and 400U/mL) or GSH (100, 200, and 400mM) at a final concentration of 50×106sperm/mL. Sperm kinematics, which was analyzed by computer-assisted sperm analysis (CASA), and membrane functionality, which was analyzed using the hypo-osmotic swelling test (HOST), were determined after the addition of the semen samples at different processing times (fresh/diluted, 1.5, 6, 12, and 24h, at 5°C). No significant differences were recorded in the kinematics or membrane functionality between treatments at different times. The supplementation of diluents with 100 and 200U/mL of CAT prevented the harmful effects of cooling on total sperm motility. No significant differences were observed in progressive sperm motility throughout processing, regardless of the treatment and time of evaluation. Supplementation with 400mM GSH resulted in an earlier reduction (P<0.05) of total sperm motility, a decrease in rapid sperm rate and a reduction in curvilinear velocity during incubation, at 5°C. The cooling induced a reduction (P<0.05) in the percentage of sperm with a functional plasma membrane (HOST), especially after 1.5h of incubation. Based on the results of the present study, the addition of CAT (100 and 200U/mL) reduced the deleterious effects of cooling on total motility in ram sperm maintained at 5°C for 24h, although it did not affect the functionality of the sperm membranes. However, the addition of 400mM GSH caused negative effects on the velocity parameters of the sperm

Neurocysticercosis: treatment with albendazole and dextrochloropheniramine (Preliminary Report)

Submitted by Guilherme Lemeszenski ([email protected]) on 2013-08-22T19:03:59Z No. of bitstreams: 1 S0036-46651988000500010.pdf: 401601 bytes, checksum: 9cf04531b0af31ba123b3d3b180ab9cc (MD5)Made available in DSpace on 2013-08-22T19:03:59Z (GMT). No. of bitstreams: 1 S0036-46651988000500010.pdf: 401601 bytes, checksum: 9cf04531b0af31ba123b3d3b180ab9cc (MD5) Previous issue date: 1988-10-01Made available in DSpace on 2013-09-30T20:06:31Z (GMT). No. of bitstreams: 2 S0036-46651988000500010.pdf: 401601 bytes, checksum: 9cf04531b0af31ba123b3d3b180ab9cc (MD5) S0036-46651988000500010.pdf.txt: 11866 bytes, checksum: 3120b4bb760218d1fe810a91055ac5d3 (MD5) Previous issue date: 1988-10-01Submitted by Vitor Silverio Rodrigues ([email protected]) on 2014-05-20T13:36:03Z No. of bitstreams: 2 S0036-46651988000500010.pdf: 401601 bytes, checksum: 9cf04531b0af31ba123b3d3b180ab9cc (MD5) S0036-46651988000500010.pdf.txt: 11866 bytes, checksum: 3120b4bb760218d1fe810a91055ac5d3 (MD5)Made available in DSpace on 2014-05-20T13:36:03Z (GMT). No. of bitstreams: 2 S0036-46651988000500010.pdf: 401601 bytes, checksum: 9cf04531b0af31ba123b3d3b180ab9cc (MD5) S0036-46651988000500010.pdf.txt: 11866 bytes, checksum: 3120b4bb760218d1fe810a91055ac5d3 (MD5) Previous issue date: 1988-10-01UNESP Faculty of Medicine of Botucatu Department of Neurology and PsychiatryUNESP Faculty of Medicine of Botucatu Department of Infectious and Parasitic DiseasesUNESP) Faculty of Medicine of Botucatu Department of Ophthalmology and OtorhinolaryngologyUNESP Faculty of Medicine of BotucatuUNESP Faculty of Medicine of Botucatu Department of Neurology and PsychiatryUNESP Faculty of Medicine of Botucatu Department of Infectious and Parasitic DiseasesUNESP) Faculty of Medicine of Botucatu Department of Ophthalmology and OtorhinolaryngologyUNESP Faculty of Medicine of Botucat

Alterations in muscular enzymes of horses competing long-distance endurance rides under tropical climate Alterações em enzimas musculares de cavalos em competições de enduro de longa distância em clima tropical

This study tried to monitor the alterations of muscle enzymes activity - creatinokinase (CK), lactate dehydrogenase (LDH), and aspartate aminotransferase (AST) - in a group of horses that participated of 70 and 100km distance rides in five competitions of an annual endurance championship under tropical climate. Pre ride levels (U/l) were 245.13±9.84 for CK, 496.61±14.76 for LDH, and 328.95±8.65 for AST. When compared to these levels, the results revealed a significant decrease in all enzymes activities in the first moment of the rides. Peak levels, significantly different, were reached, immediately after rides by CK (413.591±50.75); 24 hours post rides by LDH (628.61±33.30); and 48 hours after rides by AST (389.89±16.96). Monitoring of recovery period revealed different behavior among enzymes activities with CK values returning to pre ride values (279.61±23.05) 24 hours post rides, while LDH and AST values returned to pre ride values (505.25±33.78 and 359.35±24.90, respectively) 72 hours post rides. These data revealed different alterations in concentration of muscular enzymes in endurance horses directly related to the duration of the effort.<br>Estudaram-se as alterações de atividade das enzimas musculares creatino quinase (CK), lactato desidrogenase (LDH) e aspartato aminotransferase (AST) em um grupo de cavalos que utilizados em provas de enduro de 70 e 100km de distância, em cinco competições. Os valores (U/l) basais (antes da largada) foram 245,13±9,84 para CK, 496,61±14,76 para LDH e 328,95±8,65 para AST. Todas as atividades das enzimas decresceram no primeiro momento das provas (~30km). Valores de pico, significativamente diferentes, foram alcançados para CK (413,59±50,75) imediatamente após 70km de distância; 24 horas após para LDH (628,61±33,30); e 48 horas após as provas para AST (389,89±16,96). A monitoração do período de recuperação revelou diferente comportamento entre as concentrações enzimáticas com CK retornando aos valores basais 24 horas pós-provas (279,61 ± 23,05). LDH e AST retornaram aos valores basais, 72 horas pós-provas (505,25±33,78 e 359,35±24,90, respectivamente). Os dados obtidos revelaram diferentes alterações na concentração de enzimas musculares de cavalos de enduro, diretamente relacionadas com a duração do esforço