Comparative Analysis of V-Akt Murine Thymoma Viral Oncogene Homolog 3 (AKT3) Gene between Cow and Buffalo Reveals Substantial Differences for Mastitis

AKT3 gene is a constituent of the serine/threonine protein kinase family and plays a crucial role in synthesis of milk fats and cholesterol by regulating activity of the sterol regulatory element binding protein (SREBP). AKT3 is highly conserved in mammals and its expression levels during the lactation periods of cattle are markedly increased. AKT3 is highly expressed in the intestine followed by mammary gland and it is also expressed in immune cells. It is involved in the TLR pathways as effectively as proinflammatory cytokines. The aims of this study were to investigate the sequences differences between buffalo and cow. Our results showed that there were substantial differences between buffalo and cow in some exons and noteworthy differences of the gene size in different regions. We also identified the important consensus sequence motifs, variation in 2000 upstream of ATG, substantial difference in the “3′UTR” region, and miRNA association in the buffalo sequences compared with the cow. In addition, genetic analyses, such as gene structure, phylogenetic tree, position of different motifs, and functional domains, were performed to establish their correlation with other species. This may indicate that a buffalo breed has potential resistance to disease, environment changes, and airborne microorganisms and some good production and reproductive traits

DEVELOPMENT OF WHEAT GERMPLASM FOR STEM RUST RESISTANCE IN EASTERN AFRICA

Wheat ( Triticum aestivum ) rust outbreak is the primary production constraint in Eastern Africa. Ethiopia, Kenya and Uganda are hot spots for the epidemic of rusts, due to higher rates of evolution of new pathogen races, especially of the virulent stem rust (Puccinia graminis) race, Ug99. The objective of this study was to identify sources of resistance to the major pathotypes of stem rust prevalent in some countries of Eastern Africa. Three hundred and six elite breeding lines, selected and advanced at the Wheat Regional Centre of Excellence (WRCoE) in Ethiopia, were planted in stem rust hot spot areas of the country (Arsi-Robe) and Kenya (Njoro) under natural infections. Stem rust scores of 18, 25.8 and 56.2% of lines at Arsi-Robe; and 35, 49 and 16.7% of lines at Njoro were resistant to moderately resistant, intermediate and moderately susceptible to susceptible category, respectively. Overall coefficient of infection (ACI) at Arsi-Robe (24) was greater than that of Njoro (13), indicating higher disease pressure in Ethiopia than Kenya. Among the lines, 67% at Njoro and 49% at Arsi Robe showed good levels of resistance, with a severity less than 20%. However, only 32% of the lines showed a good level of resistance (<20% severity) in both locations. These lines could have combined resistance to the multiple stem rust races prevailing at both locations. Generally, most of the lines, which showed better resistance at Njoro were susceptible at Arsi-Robe.La rouille de la tige chez le bl\ue9 ( Triticum aestivum ) est l\u2019un des plus importants probl\ue8mes rencontres dans la production du bl\ue9 en Afrique de l\u2019Est. L\u2019Ethiopie, le Kenya et l\u2019Ouganda sont des pays \ue0 fort taux d\u2019\ue9pid\ue9mie de rouille de la tige. Ceci \ue0 cause du taux \ue9lev\ue9 de d\u2019agents pathog\ue8nes, ainsi que l\u2019apparition de nouveaux pathog\ue8nes, dont le plus redoutable (Puccinia graminis) race Ug99. L\u2019objectif de cette \ue9tude \ue9tait d\u2019identifier des sources de r\ue9sistance aux pathotypes majeurs de la rouille de tige qui sevit dans certains pays de l\u2019Afrique de l\u2019Est. Trois cent six lign\ue9es \ue9lites, s\ue9lectionn\ue9es au centre r\ue9gionale d\u2019excellence du bl\ue9 (WRCoE) en Ethiopie, ont \ue9t\ue9 plant\ue9es dans des zones a fort taux de pr\ue9valence de la rouille de tige en Ethiopie (Arsi-Robe) au Kenya (Njoro). Respectivement, 18, 25.8 et 56.2% des lign\ue9es a Arsi-Robe; et 35, 49 et 16.7% des lign\ue9es a Njoro se sont r\ue9v\ue9l\ue9es r\ue9sistantes ou mod\ue9r\ue9ment r\ue9sistantes, interm\ue9diaires, mod\ue9r\ue9ment susceptibles a susceptibles. En somme, le coefficient d\u2019infection (ACI) \ue0 Arsi-Robe (24) \ue9tait plus \ue9lev\ue9 qu\u2019\ue0 Njoro (13), ceci indique que la pression de la rouille de tige est plus \ue9lev\ue9e en Ethiopie qu\u2019au Kenya. Parmi les lign\ue9es \ue9valu\ue9es, 67% \ue0 Njoro et 49% a Arsi Robe sont exhibe des bons niveaux de r\ue9sistance, avec 20% de s\ue9v\ue9rit\ue9. N\ue9anmoins, seul 32% des lign\ue9es ont exhibe une bonne r\ue9sistance (<20% s\ue9v\ue9rit\ue9) dans les deux localit\ue9s. Ces lign\ue9es porteraient surement des g\ue8nes de r\ue9sistance multiple contre les diff\ue9rentes races de champignon pr\ue9sents dans les deux localit\ue9s. G\ue9n\ue9ralement, la plupart des lign\ue9es ayant exhibe une bonne r\ue9sistance a Njoro \ue9taient susceptible a Arsi-Robe

Effect of a self-care educational intervention to improve self-care adherence among patients with chronic heart failure: a clustered randomized controlled trial in Northwest Ethiopia

Background: As the burden of cardiovascular disease increases in sub-Saharan Africa, there is a growing need for low-cost interventions to mitigate its impact. Providing self-care health education to patients with chronic heart failure (CHF) is recommended as an intervention to prevent complications, improve quality of life, and reduce financial burdens on fragile health systems. However, little is known about health education’s effectiveness at improving CHF self-management adherence in sub-Saharan Africa. Therefore the present study aimed to assess the effectiveness of an educational intervention to improve self-care adherence among patients with CHF at Debre Markos and Felege Hiwot Referral Hospitals in Northwest Ethiopia. Methods: To address this gap, we adapted a health education intervention based on social cognitive theory comprising of intensive four-day training and, one-day follow-up sessions offered every four months. Patients also received illustrated educational leaflets. We then conducted a clustered randomized control trial of the intervention with 186 randomly-selected patients at Debre Markos and Felege Hiwot referral hospitals. We collected self-reported data on self-care behavior before each educational session. We analyzed these data using a generalized estimating equations model to identify health education's effect on a validated 8-item self-care adherence scale. Results: Self-care adherence scores were balanced at baseline. After the intervention, patients in the intervention group (n = 88) had higher adherence scores than those in the control group (n = 98). This difference was statistically significant (β = 4.15, p < 0.05) and increased with each round of education. Other factors significantly associated with adherence scores were being single (β = − 0.25, p < 0.05), taking aspirin (β = 0.76, p < 0.05), and having a history of hospitalization (β = 0.91, p < 0.05). Conclusions: We find that self-care education significantly improved self-care adherence scores among CHF patients. This suggests that policymakers should consider incorporating self-care education into CHF management

Risk factors of visceral leishmaniasis in East Africa: a case-control study in Pokot territory of Kenya and Uganda

BACKGROUND: In East Africa, visceral leishmaniasis (VL) is endemic in parts of Sudan, Ethiopia, Somalia, Kenya and Uganda. It is caused by Leishmania donovani and transmitted by the sandfly vector Phlebotomus martini. In the Pokot focus, reaching from western Kenya into eastern Uganda, formulation of a prevention strategy has been hindered by the lack of knowledge on VL risk factors as well as by lack of support from health sector donors. The present study was conducted to establish the necessary evidence-base and to stimulate interest in supporting the control of this neglected tropical disease in Uganda and Kenya. METHODS: A case-control study was carried out from June to December 2006. Cases were recruited at Amudat hospital, Nakapiripirit district, Uganda, after clinical and parasitological confirmation of symptomatic VL infection. Controls were individuals that tested negative using a rK39 antigen-based dipstick, which were recruited at random from the same communities as the cases. Data were analysed using conditional logistic regression. RESULTS: Ninety-three cases and 226 controls were recruited into the study. Multivariate analysis identified low socio-economic status and treating livestock with insecticide as risk factors for VL. Sleeping near animals, owning a mosquito net and knowing about VL symptoms were associated with a reduced risk of VL. CONCLUSIONS: VL affects the poorest of the poor of the Pokot tribe. Distribution of insecticide-treated mosquito nets combined with dissemination of culturally appropriate behaviour-change education is likely to be an effective prevention strategy

Adsorption of chromium from electroplating wastewater using activated carbon developed from water hyacinth

Abstract Industrial wastewater polluted with high concentrations of Cr is commonly discharged into water resources without proper treatment. This gives rise to the deterioration of water quality and imposes adverse effects on public health. Therefore, this study is aimed at removing Cr from electroplating wastewater using activated carbon produced from water hyacinth under a full factorial experimental design with three factors and three levels (pH,2,5 and 8, adsorbent dose 0.5,1and1.5 in 100 mL and contact time 30, 60 and120 min). A phosphoric acid solution of 37% was used to activate the carbon, which was then subjected to thermal decomposition for 15 min at 500 °C. The activated carbon was characterized by the presence of a high surface area (203.83 m2/g) of BET, cracking of adsorbent beads of SEM morphology, amorphous nature of XRD, and many functional groups of FTIR such as hydroxyl (3283 cm−1), alkane (2920 cm−1), nitrile (2114 cm−1) and aromatics (1613 cm−1). The minimum Cr adsorption performance of 15.6% was obtained whereas maximum removal of 90.4% was recorded at the experimental condition of pH 2, adsorbent dose of 1.5 g/100 mL, and contact time of 120 min at a fixed value of initial Cr concentration of 100 mg/L. Similarly, the maximum Cr removal from real electroplating wastewater was 81.2% at this optimum point. Langmuir's model best described the experimental value at R2 0.96 which implies the adsorption is chemically bonded, homogeneous, and monolayer. Pseudo-second-order model best fits with the experimental data with R2 value of 0.99. The adsorbent was regenerated for seven cycles and the removal efficiency decreased from 93.25% to 21.35%. Finally, this technology is promising to be scaled up to an industrial level

Respectful maternity care in Ethiopian public health facilities

Background: Disrespect and abuse of women during institutional childbirth services is one of the deterrents to utilization of maternity care services in Ethiopia and other low- and middle-income countries. This paper describes the prevalence of respectful maternity care (RMC) and mistreatment of women in hospitals and health centers, and identifies factors associated with occurrence of RMC and mistreatment of women during institutional labor and childbirth services. Methods: This study had a cross sectional study design. Trained external observers assessed care provided to 240 women in 28 health centers and hospitals during labor and childbirth using structured observation checklists. The outcome variable, providers' RMC performance, was measured by nine behavioral descriptors. The outcome, any mistreatment, was measured by four items related to mistreatment of women: physical abuse, verbal abuse, absence of privacy during examination and abandonment. We present percentages of the nine RMC indicators, mean score of providers' RMC performance and the adjusted multilevel model regression coefficients to determine the association with a quality improvement program and other facility and provider characteristics. Results: Women on average received 5.9 (66%) of the nine recommended RMC practices. Health centers demonstrated higher RMC performance than hospitals. At least one form of mistreatment of women was committed in 36% of the observations (38% in health centers and 32% in hospitals). Higher likelihood of performing high level of RMC was found among male vs. female providers ((beta) over cap = 0: 65, p = 0.012), midwives vs. other cadres ((beta) over cap = 0: 88, p = 0.002), facilities implementing a quality improvement approach, Standards-based Management and Recognition (SBM-R (c)) ((beta) over cap = 1: 31, p = 0.003), and among laboring women accompanied by a companion (beta) over cap = 0: 99, p = 0.003). No factor was associated with observed mistreatment of women. Conclusion: Quality improvement using SBM-R (c) and having a companion during labor and delivery were associated with RMC. Policy makers need to consider the role of quality improvement approaches and accommodating companions in promoting RMC. More research is needed to identify the reason for superior RMC performance of male providers over female providers and midwives compared to other professional cadre, as are longitudinal studies of quality improvement on RMC and mistreatment of women during labor and childbirth services in public health facilities

African linguistics on the prairie: Selected papers from the 45th Annual Conference on African Linguistics

African Linguistics on the Prairie&nbsp;features select revised peer-reviewed papers from the 45th Annual Conference on African Linguistics, held at the University of Kansas. The articles in this volume reflect the enormous diversity of African languages, as they focus on languages from all of the major African language phyla.&nbsp; The articles here also reflect the many different research perspectives that frame the work of linguists in the Association for Contemporary African Linguistics. The diversity of views presented in this volume are thus indicative of the vitality of current African linguistics research. The work presented in this volume represents both descriptive and theoretical methodologies and covers fields ranging from phonetics, phonology, morphology, typology, syntax, and semantics to sociolinguistics, discourse analysis, language acquisition, computational linguistics and beyond.&nbsp; This broad scope and the quality of the articles contained within holds out the promise of continued advancement in linguistic research on African languages

African linguistics on the prairie: Selected papers from the 45th Annual Conference on African Linguistics

African Linguistics on the Prairie&nbsp;features select revised peer-reviewed papers from the 45th Annual Conference on African Linguistics, held at the University of Kansas. The articles in this volume reflect the enormous diversity of African languages, as they focus on languages from all of the major African language phyla.&nbsp; The articles here also reflect the many different research perspectives that frame the work of linguists in the Association for Contemporary African Linguistics. The diversity of views presented in this volume are thus indicative of the vitality of current African linguistics research. The work presented in this volume represents both descriptive and theoretical methodologies and covers fields ranging from phonetics, phonology, morphology, typology, syntax, and semantics to sociolinguistics, discourse analysis, language acquisition, computational linguistics and beyond.&nbsp; This broad scope and the quality of the articles contained within holds out the promise of continued advancement in linguistic research on African languages