TT2013 meeting report: the transgenic technology meeting visits Asia for the first time

The 11th Transgenic Technology meeting was held in Guangzhou, China on 25th–27th February 2013. Over 300 scientists and students from round the world gathered to hear the latest developments in the technologies underpinning the creation of transgenic and knockout animals and their application to biological sciences in areas such as the modeling human diseases and biotechnology. As well as informative presentations from leading researchers in the field, an excellent selection of short talks selected from abstracts and posters, attendees were also treated to an inspiring talk from Allan Bradley who was awarded the 9th International Society of Transgenic Technologies Prize for outstanding contributions to the field of transgenic technologies

Shaping Future Generations

Due to the extent and variety of research available on the topic of child-targeted advertising, it was determined that an appraisal of the current state of the situation was necessary in order to determine the best course of action. After summarizing the history of children’s advertising and considering the arguments on both sides of the debate – from the children’s advocates and the advertising industry – the previously recommended solutions were evaluated in the paper. Each of these possible solutions were considered, and led to the proposal of a best solution. Supported by research, the best solution advocated a joint effort between the children’s advocates, parents and the advertising industry that emphasized education over government restriction

Mouse tafazzin is required for male germ cell meiosis and spermatogenesis

Barth syndrome is an X-linked mitochondrial disease, symptoms of which include neutropenia and cardiac myopathy. These symptoms are the most significant clinical consequences of a disease, which is increasingly recognised to have a variable presentation. Mutation in the Taz gene in Xq28 is thought to be responsible for the condition, by altering mitochondrial lipid content and mitochondrial function. Male chimeras carrying a targeted mutation of Taz on their X-chromosome were infertile. Testes from the Taz knockout chimeras were smaller than their control counterparts and this was associated with a disruption of the progression of spermatocytes through meiosis to spermiogenesis. Taz knockout ES cells also showed a defect when differentiated to germ cells in vitro. Mutant spermatocytes failed to progress past the pachytene stage of meiosis and had higher levels of DNA double strand damage and increased levels of endogenous retrotransposon activity. Altogether these data revealed a novel role for Taz in helping to maintain genome integrity in meiosis and facilitating germ cell differentiation. We have unravelled a novel function for the Taz protein, which should contribute to an understanding of how a disruption of the Taz gene results in the complex symptoms underlying Barth Syndrome

Dissociative Autoionization in (1+2)-photon Above Threshold Excitation of H2 Molecules

We have theoretically studied the effect of dissociative autoionization on the photoelectron energy spectrum in (1+2)-photon above threshold ionization(ATI) of H2 molecules. We have considered excitation from the ground state X-singlet-Sigma-g+(v=0,j) to the doubly excited autoionizing states of singlet-Sigma-u+ and singlet-Pi-u+ symmetry, via the intermediate resonant B-singlet-Sigma-u+(v=5,j) states. We have shown that the photoelectron energy spectrum is oscillatory in nature and shows three distinct peaks above the photoelectron energy 0.7 eV. This feature has been observed in a recent experiment by Rottke et al, J. Phys. B, Vol. 30, p-4049 (1997).Comment: 11 pages and 4 figure

The initiator methionine tRNA drives cell migration and invasion leading to increased metastatic potential in melanoma

The cell's repertoire of transfer RNAs (tRNAs) has been linked to cancer. Recently, levels of the initiator methionine tRNA (tRNAiMet) in stromal fibroblasts have been shown to influence extracellular matrix (ECM) secretion to drive tumour growth and angiogenesis. Here we show that increased tRNAiMet within cancer cells does not influence tumour growth, but drives cell migration and invasion via a mechanism that is independent from ECM synthesis and dependent on α5β1 integrin and levels of the translation initiation ternary complex. In vivo and ex vivo migration (but not proliferation) of melanoblasts is significantly enhanced in transgenic mice which express additional copies of the tRNAiMet gene. We show that increased tRNAiMet in melanoma drives migratory, invasive behaviour and metastatic potential without affecting cell proliferation and primary tumour growth, and that expression of RNA polymerase III-associated genes (which drive tRNA expression) are elevated in metastases by comparison with primary tumours. Thus specific alterations to the cancer cell tRNA repertoire drive a migration/invasion programme that may lead to metastasis

Gauge Dependence of Mass and Condensate in Chirally Asymmetric Phase of Quenched QED3

We study three dimensional quenched Quantum Electrodynamics in the bare vertex approximation. We investigate the gauge dependence of the dynamically generated Euclidean mass of the fermion and the chiral condensate for a wide range of values of the covariant gauge parameter $\xi$. We find that (i) away from $\xi=0$, gauge dependence of the said quantities is considerably reduced without resorting to sophisticated vertex {\em ansatze}, (ii) wavefunction renormalization plays an important role in restoring gauge invariance and (iii) the Ward-Green-Takahashi identity seems to increase the gauge dependence when used in conjunction with some simplifying assumptions. In the Landau gauge, we also verify that our results are in agreement with those based upon dimensional regularization scheme within the numerical accuracy available.Comment: 14 pages, 11 figures, uses revte

Genome-scale analysis identifies paralog lethality as a vulnerability of chromosome 1p loss in cancer.

Functional redundancy shared by paralog genes may afford protection against genetic perturbations, but it can also result in genetic vulnerabilities due to mutual interdependency1-5. Here, we surveyed genome-scale short hairpin RNA and CRISPR screening data on hundreds of cancer cell lines and identified MAGOH and MAGOHB, core members of the splicing-dependent exon junction complex, as top-ranked paralog dependencies6-8. MAGOHB is the top gene dependency in cells with hemizygous MAGOH deletion, a pervasive genetic event that frequently occurs due to chromosome 1p loss. Inhibition of MAGOHB in a MAGOH-deleted context compromises viability by globally perturbing alternative splicing and RNA surveillance. Dependency on IPO13, an importin-β receptor that mediates nuclear import of the MAGOH/B-Y14 heterodimer9, is highly correlated with dependency on both MAGOH and MAGOHB. Both MAGOHB and IPO13 represent dependencies in murine xenografts with hemizygous MAGOH deletion. Our results identify MAGOH and MAGOHB as reciprocal paralog dependencies across cancer types and suggest a rationale for targeting the MAGOHB-IPO13 axis in cancers with chromosome 1p deletion

Supersymmetric branes with (almost) arbitrary tensions

We present a supersymmetric version of the two-brane Randall-Sundrum scenario, with arbitrary brane tensions T_1 and T_2, subject to the bound |T_{1,2}| \leq \sqrt{-6\Lambda_5}, where \Lambda_5 < 0 is the bulk cosmological constant. Dimensional reduction gives N=1, D=4 supergravity, with cosmological constant \Lambda_4 in the range \half\Lambda_5 \leq \Lambda_4 \leq 0. The case with \Lambda_4 = 0 requires T_1 = -T_2 = \sqrt{-6\Lambda_5}. This work unifies and generalizes previous approaches to the supersymmetric Randall-Sundrum scenario. It also shows that the Randall-Sundrum fine-tuning is not a consequence of supersymmetry.Comment: 19pp; Published versio

Evaluating the impact of Mexico’s drug policy reforms on people who inject drugs in Tijuana, B.C., Mexico, and San Diego, CA, United States: a binational mixed methods research agenda

Background: Policymakers and researchers seek answers to how liberalized drug policies affect people who inject drugs (PWID). In response to concerns about the failing “war on drugs,” Mexico recently implemented drug policy reforms that partially decriminalized possession of small amounts of drugs for personal use while promoting drug treatment. Recognizing important epidemiologic, policy, and socioeconomic differences between the United States—where possession of any psychoactive drugs without a prescription remains illegal—and Mexico—where possession of small quantities for personal use was partially decriminalized, we sought to assess changes over time in knowledge, attitudes, behaviors, and infectious disease profiles among PWID in the adjacent border cities of San Diego, CA, USA, and Tijuana, Baja California, Mexico. Methods: Based on extensive binational experience and collaboration, from 2012–2014 we initiated two parallel, prospective, mixed methods studies: Proyecto El Cuete IV in Tijuana (n = 785) and the STAHR II Study in San Diego (n = 575). Methods for sampling, recruitment, and data collection were designed to be compatible in both studies. All participants completed quantitative behavioral and geographic assessments and serological testing (HIV in both studies; hepatitis C virus and tuberculosis in STAHR II) at baseline and four semi-annual follow-up visits. Between follow-up assessment visits, subsets of participants completed qualitative interviews to explore contextual factors relating to study aims and other emergent phenomena. Planned analyses include descriptive and inferential statistics for quantitative data, content analysis and other mixed-methods approaches for qualitative data, and phylogenetic analysis of HIV-positive samples to understand cross-border transmission dynamics. Results: Investigators and research staff shared preliminary findings across studies to provide feedback on instruments and insights regarding local phenomena. As a result, recruitment and data collection procedures have been implemented successfully, demonstrating the importance of binational collaboration in evaluating the impact of structural-level drug policy reforms on the behaviors, health, and wellbeing of PWID across an international border. Conclusions: Our prospective, mixed methods approach allows each study to be responsive to emerging phenomena within local contexts while regular collaboration promotes sharing insights across studies. The strengths and limitations of this approach may serve as a guide for other evaluations of harm reduction policies internationally

Higher Loop Spin Field Correlators in Various Dimensions

We compute higher-point superstring correlators involving spin fields in various even space-time dimensions D at tree-level and to arbitrary loop order. This generalizes previous work in D=4 space-time dimensions. The main focus are D=6,8 and D=10 superstring compactifications for which correlation functions with four and more spin fields are computed. More precisely, we present every non-vanishing six-point function. A number of results can even be derived for arbitrary D. A closed formula for the correlators with any number of fermions psi and two spin fields S in D space-time dimensions is given for arbitrary genus. Moreover, in D=6 and for arbitrary genus, we find a general formula for the correlators . The latter serve as basic building blocks to construct higher-point fermionic correlation functions. In D=8 we can profit from the SO(8)-triality to derive further tree-level correlators with a large numbers of spin fields.Comment: 47 pages, 1 figure; v2: typos corrected; submitted to JHE