Effect of diethyl maleate on toxicity of linalool against two stored product insects in laboratory condition

Essential oil extracted from plants has been widely investigated for pest control properties, with some proving to be toxic in insect pests. In this study, effect of the synergist diethyl maleate (DEM) on toxicity of one monoterpenoids, linalool was studied against two most common stored-product insects such as Callosobruchus maculatus and Rhyzopertha dominica. Diethyl maleate was combined in mass ratios (1:8 and 1:4) with acetone used and applied on C. maculatus and R. dominica adult. Five concentrations of linalool were tested with four replications at 24 and 48 h with 30 adult insect in each replication. After 24 h of exposure, the LC50 values were estimated to be 23.61 and 31.01 μl/l air, and after 48 h, they were 15.07 and 21.84 for each insect, respectively. A combination of inalool with the synergist after 24 h of exposure, the LC50 values was estimated to be 11.93 and 13.07 μl/l air and after 48 h, they were 7.38 and 7.93, respectively for each insect. The synergist is able to block the specific system of enzymes involved in selection of tolerance in susceptible generations. Diethyl maleate is an inhibitor of glutathione S-transferase (GST) activity. These results show that diethyl maleate decrease doses of linalool.Key words: Diethyl maleate, linalool, LC50, synergism, Rhyzopertha dominica, Callosobruchus maculatus

Report of Erythraeus (Erythraeus) adanaensis (Acari: Prostigmata: Erythraeidae) from Iran

Based on a study on the Parasitengona mite fauna of East Azarbaijan province (Iran) accomplished in 2007 and 2008, several specimens were collected. Among them, Erythraeus (Erythraeus) adanaensis Saboori & Cobanoglu, 2010 is getting reported for the first time from Iran

Identification and Pathway Analysis of microRNAs with No Previous Involvement in Breast Cancer

microRNA expression signatures can differentiate normal and breast cancer tissues and can define specific clinico-pathological phenotypes in breast tumors. In order to further evaluate the microRNA expression profile in breast cancer, we analyzed the expression of 667 microRNAs in 29 tumors and 21 adjacent normal tissues using TaqMan Low-density arrays. 130 miRNAs showed significant differential expression (adjusted P value = 0.05, Fold Change = 2) in breast tumors compared to the normal adjacent tissue. Importantly, the role of 43 of these microRNAs has not been previously reported in breast cancer, including several evolutionary conserved microRNA*, showing similar expression rates to that of their corresponding leading strand. The expression of 14 microRNAs was replicated in an independent set of 55 tumors. Bioinformatic analysis of mRNA targets of the altered miRNAs, identified oncogenes like ERBB2, YY1, several MAP kinases, and known tumor-suppressors like FOXA1 and SMAD4. Pathway analysis identified that some biological process which are important in breast carcinogenesis are affected by the altered microRNA expression, including signaling through MAP kinases and TP53 pathways, as well as biological processes like cell death and communication, focal adhesion and ERBB2-ERBB3 signaling. Our data identified the altered expression of several microRNAs whose aberrant expression might have an important impact on cancer-related cellular pathways and whose role in breast cancer has not been previously described

NAViGaTing the Micronome – Using Multiple MicroRNA Prediction Databases to Identify Signalling Pathway-Associated MicroRNAs

MicroRNAs are a class of small RNAs known to regulate gene expression at the transcript level, the protein level, or both. Since microRNA binding is sequence-based but possibly structure-specific, work in this area has resulted in multiple databases storing predicted microRNA:target relationships computed using diverse algorithms. We integrate prediction databases, compare predictions to in vitro data, and use cross-database predictions to model the microRNA:transcript interactome--referred to as the micronome--to study microRNA involvement in well-known signalling pathways as well as associations with disease. We make this data freely available with a flexible user interface as our microRNA Data Integration Portal--mirDIP (http://ophid.utoronto.ca/mirDIP).mirDIP integrates prediction databases to elucidate accurate microRNA:target relationships. Using NAViGaTOR to produce interaction networks implicating microRNAs in literature-based, KEGG-based and Reactome-based pathways, we find these signalling pathway networks have significantly more microRNA involvement compared to chance (p<0.05), suggesting microRNAs co-target many genes in a given pathway. Further examination of the micronome shows two distinct classes of microRNAs; universe microRNAs, which are involved in many signalling pathways; and intra-pathway microRNAs, which target multiple genes within one signalling pathway. We find universe microRNAs to have more targets (p<0.0001), to be more studied (p<0.0002), and to have higher degree in the KEGG cancer pathway (p<0.0001), compared to intra-pathway microRNAs.Our pathway-based analysis of mirDIP data suggests microRNAs are involved in intra-pathway signalling. We identify two distinct classes of microRNAs, suggesting a hierarchical organization of microRNAs co-targeting genes both within and between pathways, and implying differential involvement of universe and intra-pathway microRNAs at the disease level

Comparative Study of Feeding on Some Pollens on Biology of Typhlodromus bagdasarjani Arutunjian & Wainstein (Acari: Phytoseiidae)

In this research, effect of feeding of five pollen sources, apple, pear, almond, apricot and walnut, as 5 treatments on the period of egg to adult, longevity and fecundity of the predatory mite, Typhlodromus bagdasarjani, was studied in a complete randomized design (CRD). Mites kept individually on black mulberry leaf discs at 24±2 °C temperature, 60±5 % relative humidity and a photoperiod of 16:8 (L:D) hours, with a sufficient quantity of the pollens. Results showed that T. bagdasarjani could develop and reproduce when the predatory mite feed on the all of diets. Thus, the pollens can be alternative foods for the mite. The mean of developmental time from egg to adult emergence varied between the treatments from 11.69 to 21.49 days for females and 11.70 to 20.07 days for males. The minimum mean of developmental time of females and males was on walnut, almond and apple pollens and the maximum mean of it was on pear pollen. The mean of longevity varied among the treatments from 17.45 to 31.26 days for the females and 18.23 to 31.44 days for the male insects. The maximum mean longevity was on apricot and almond pollens for females and it had maximum amount on apricot and walnut for males. The minimum mean of longevity of females and males was on pear pollen. Also the maximum means of daily and total fecundity (0.73 and 9.60 eggs/female) were on apple and walnut pollens. The minimum amount of these means was on pear pollen. According to these results, almond and walnut pollens were the most suitable diet for T. bagdasarjani as compared to the other pollens. Among diets, pear pollen had least desirability and nutritional value for the mite

REDESCRIPTION OF TYPHLOSEIULUS CARMONAE (CHANT AND YOSHIDA- SHAUL) (MESOSTIGMATA: PHYTOSEIIDAE) NEW SPECIES FOR IRAN

Typhloseiulus carmonae (Chant et Yoshida-Shaul, 1983) recorded in Iran for the first time is redescribed. The external morphology of the specimens from Iran is compared with those given in the original description from Portugal (Chant and Yoshida-Shaul 1983) and redescription from Spain (Ferragut 1991). Females of this species from Iran differ from those from other two localities by the fixed digit of chelicerae with 3 teeth (vs 2). In males from Iran, setae of dorsal shield are significantly longer than those of allotype from Portugal. A key to the species of Typhloseiulus spp. recorded from Iran is given

Evaluation and comparison of different models for asphaltene particle deposition in flow streams

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Asphaltene deposition in the wellbore is a frequent problem in certain oil fields that may yield serious operational challenges, safety hazards, and an overall decrease in production efficiency. These particulates are entrained in the flowing fluid from the reservoir or generated in the wellbore flow. The asphaltene precipitation is a function of pressure, temperature, and fluid composition. Considering the existence of asphaltene in the oil, the accurate prediction of those solid particle deposition is necessary to design production facilities and to optimize well performance. A great deal of research has been conducted to study the dynamic aspects of solid particles fouling inside a pipe. However, those studies have mostly been applied in engineering fields other than petroleum. In this paper, firstly we discuss the mechanisms for solid particle deposition, including particle momentum and mass transfer as well as particle re-entrainment. Afterwards we describe important particulate deposition models and compare them using different sets of experiments published in the literature. In one set, deposition of iron particles in the gas flow is investigated and in the other, deposition of asphaltene in the oil flow is considered. Our results show that the deposition models that were studied in this work satisfactorily agree with experimental data. They are also applicable for modeling asphaltene deposition in crude oils as well as other particle deposition in gas flow streams. (C) 2012 Elsevier B.V. All rights reserved.84-855771Abu Dhabi National Oil Company (ADNOC)Center for Petroleum and Geosystems Engineering of The University of Texas at AustinCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES

Psychological symptoms and body image in individuals with gender dysphoria: A comparison between Iranian and Dutch clinics

Background: Few studies have compared the psychological functioning of individuals with gender dysphoria in Western and non-Western cultures. To our knowledge, this is the first study comparing the mental health of transgender individuals from an Islamic and non-Islamic country (Iran and the Netherlands). Methods: In this study, the psychological functioning and body image of 163 individuals with gender dysphoria (100 transgender women (75 in the Netherlands, 25 in Iran) and 63 transgender men (45 in the Netherlands, 18 in Iran) in two clinics located in Iran (N = 43) and the Netherlands (N = 120) was evaluated using the SCL-90 and the Body Image Scale (BIS). Also, none of these individuals had yet received hormonal therapy and/or surgery in their clinics. Results: Dutch participants (M = 31.56, SD = 12.26) were older than Iranian participants (M = 25.21, SD = 3.04). Dutch transwomen were less often androphilic (sexually attracted to men) than Iranian transwomen, and Iranian trans people were more often bisexual than the Dutch trans people. Significantly more Dutch transgender people were married (we had no information about the gender of the spouse), and indicated to have more contact with their families than the participants in Iran. The participants from Iran had significantly more psychological complaints than the Dutch participants. Compared to participants in Iran, participants in the Netherlands were more dissatisfied with their secondary sexual characteristics and neutral body characteristics, but there was no significant difference between the countries in terms of satisfaction with primary sex characteristics. Conclusions: Although transgender people in many countries face social and mental health problems, this study suggests that socio-cultural factors may increase the likelihood of psychopathology