Animal influence on water, sanitation and hygiene measures for zoonosis control at the household level: A systematic literature review

Neglected zoonotic diseases (NZDs) have a significant impact on the livelihoods of the world’s poorest populations, which often lack access to basic services. Water, sanitation and hygiene (WASH) programmes are included among the key strategies for achieving the World Health Organization’s 2020 Roadmap for Implementation for control of Neglected Tropical Diseases (NTDs). There exists a lack of knowledge regarding the effect of animals on the effectiveness of WASH measures. This review looked to identify how animal presence in the household influences the effectiveness of water, hygiene and sanitation measures for zoonotic disease control in low and middle income countries; to identify gaps of knowledge regarding this topic based on the amount and type of studies looking at this particular interaction

Oxidative protein labeling in mass-spectrometry-based proteomics

Oxidation of proteins and peptides is a common phenomenon, and can be employed as a labeling technique for mass-spectrometry-based proteomics. Nonspecific oxidative labeling methods can modify almost any amino acid residue in a protein or only surface-exposed regions. Specific agents may label reactive functional groups in amino acids, primarily cysteine, methionine, tyrosine, and tryptophan. Nonspecific radical intermediates (reactive oxygen, nitrogen, or halogen species) can be produced by chemical, photochemical, electrochemical, or enzymatic methods. More targeted oxidation can be achieved by chemical reagents but also by direct electrochemical oxidation, which opens the way to instrumental labeling methods. Oxidative labeling of amino acids in the context of liquid chromatography(LC)–mass spectrometry (MS) based proteomics allows for differential LC separation, improved MS ionization, and label-specific fragmentation and detection. Oxidation of proteins can create new reactive groups which are useful for secondary, more conventional derivatization reactions with, e.g., fluorescent labels. This review summarizes reactions of oxidizing agents with peptides and proteins, the corresponding methodologies and instrumentation, and the major, innovative applications of oxidative protein labeling described in selected literature from the last decade

Early Reactional Hyperplasia and Neuronal Differentiation of the Ciliary Epithelium (CE) in Experimental Retinal Detachment (RD) with Proliferative VitreoRetinopathy (PVR) in the Porcine Eye

Background: Rare quiescent retinal progenitor cells have been isolated in the adult amphibian ciliary marginal zone (Hollyfield, 1968), then in the mammalian (Tropepe et al, 2000) (Ahmad et al, 2000) (Fischer and Rey, 2000) and human CE (Coles et al, 2004) (Ballios et al, 2011). This contingent might be inhibited in the adult eye by the signaling pathway ephrin (Chen, personal communication, ARVO 2012, submitted, Stem Cells). Retinal injury might be a triggering factor to quit the dormant state in the adult mammalian CE (Ooto, 2004) (Nickerson, 2007) (Nishiguchi 2008) (Kiyama et Wang, 2010), as it has been demonstrated also in the human brain (Moe et al, 2005) (Logan et al, 2006) . We have reported a CE proliferation with retinal neuronal and photoreceptor differentiation in three human eyes eviscerated for longstanding RD and PVR (Ducournau et al 2012). The CE strongly expressed EGFR. The concept of niche (Fischer and Rey, 2003) is now well established in stem cells recruitment processes. We have hypothezised that the disease RD and PVR might stimulate a dormant population of Retinal Progenitor Cells (RPCs) in the CE in the human eye in vivo in presence of a niche constituted by EGF. Proliferation in the ciliary body, together with nestin expression in ciliary MCM2+ (macrophage/microglia marker F4/80) cells were found in mice eyes with experimental RD, suggesting that RD might activate putative RPCs (Suburo et al, 2010). The aim of the present work was to study the CE in the porcine eye with experimental DR and PVR

Measurement of the Mass of the Z-Boson and the Energy Calibration of Lep

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Electroweak parameters of the z0 resonance and the standard model

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