Structure of the novel membrane-coating material in proton-secreting epithelial cells and identification as an H+ATPase.

Specialized proton-secreting cells known collectively as mitochondria-rich cells are found in a variety of transporting epithelia, including the kidney collecting duct (intercalated cells) and toad and turtle urinary bladders. These cells contain a population of characteristic tubulovesicles that are believed to be involved in the shuttling of proton pumps (H+ATPase) to and from the plasma membrane. These transporting vesicles have a dense, studlike material coating the cytoplasmic face of their limiting membranes and similar studs are also found beneath parts of the plasma membrane. We have recently shown that this membrane coat does not contain clathrin. The present study was performed to determine the structure of this coat in rapidly frozen and freeze-dried tissue, and to determine whether the coat contains a major membrane protein transported by these vesicles, a proton pumping H+ATPase. The structure of the coat was examined in proton-secreting, mitochondria-rich cells from toad urinary bladder epithelium by rapidly freezing portions of apical membrane and associated cytoplasm that were sheared away from the remainder of the cell using polylysine-coated coverslips. Regions of the underside of these apical membranes as large as 0.2 micron2 were decorated by studlike projections that were arranged into regular hexagonal arrays. Individual studs had a diameter of 9.5 nm and appeared to be composed of multiple subunits arranged around a central depression, possibly representing a channel. The studs had a density of approximately 16,800 per micron2 of membrane. Similar arrays of studs were also found on vesicles trapped in the residual band of cytoplasm that remained attached to the underside of the plasma membrane, but none were seen in adjacent granular cells. To determine whether these arrays of studs contained H+ATPase molecules, we examined a preparation of affinity-purified bovine medullary H+ATPase, using the same technique, after incorporation of the protein eluted from a monoclonal antibody affinity column into phospholipid liposomes. The affinity-purified protein was shown to be capable of ATP-dependent acidification. In such preparations, large paracrystalline arrays of studs identical in appearance to those seen in situ were found. The dimensions of the studs as well as the number per square micrometer of membrane were identical to those of toad bladder mitochondria-rich cells: 9.5 nm in diameter, 16,770 per micron2 of membrane.(ABSTRACT TRUNCATED AT 400 WORDS

Research on digital transducer principles. Volume 10 - Thin film titanium dioxide by chemical vapor deposition, 1 July - 31 December 1968

Thin film titanium dioxide by high temperature vapor depositio

Representations of hom-Lie algebras

In this paper, we study representations of hom-Lie algebras. In particular, the adjoint representation and the trivial representation of hom-Lie algebras are studied in detail. Derivations, deformations, central extensions and derivation extensions of hom-Lie algebras are also studied as an application.Comment: 16 pages, multiplicative and regular hom-Lie algebras are used, Algebra and Representation Theory, 15 (6) (2012), 1081-109

Time Delay Interferometry combinations as instrument noise monitors for LISA

The LISA mission will likely be a signal dominated detector, such that one challenge is the separation of the different astrophysical sources, and to distinguish between them and the instrumental noise. One of the goals of LISA is to probe the early Universe by detecting stochastic GW backgrounds. As correlation with other detectors is not possible for LISA, discrimination of such a GW background from the instrumental noise requires a good estimate of the latter. To this purpose we have revisited Time Delay Interferometry (TDI) to look for new TDI signal combinations that fulfill the laser frequency noise suppression requirements. We illustrate that it is possible to do a linear combination of these TDI channels to find special null-combinations that suppress gravitational waves and mainly carry information about instrumental noise. We find that there exist many null-combinations that show different sensitivities to gravitational waves, some of which seem more suitable than the traditional T combination for estimating test-mass acceleration noise. In an idealised LISA configuration, they are all sensitive to a particular linear combination of the six test-masses acceleration, similar to a rigid rotation of the LISA triangle. In the following article, we illustrate what are the noise properties that can be extracted by monitoring these interferometry signals and discuss the implication of these findings for the detection of stochastic GW backgrounds

Toward an ecological aesthetics: music as emergence

In this article we intend to suggest some ecological based principles to support the possibility of develop an ecological aesthetics. We consider that an ecological aesthetics is founded in concepts as “direct perception”, “acquisition of affordances and invariants”, “embodied embedded perception” and so on. Here we will purpose that can be possible explain especially soundscape music perception in terms of direct perception, working with perception of first hand (in a Gibsonian sense). We will present notions as embedded sound, detection of sonic affordances and invariants, and at the end we purpose an experience with perception/action paradigm to make soundscape music as emergence of a self-organized system