1,284 research outputs found
Proteins to Order Use of Synthetic DNA to Generate Site-Specific Mutations
The ability to cause specific changes in the amino acid
sequences of proteins would greatly advance studies on the
influence of protein structure on biochemical function. If
the desired changes can once be made in the nucleic acid
which encodes the protein, one can use cloning in an appropriate microorganism to produce essentially limitless
quantities of the mutant protein. We describe here the
application of oligonucleotide-directed site-specific mutagenesis to accomplish this objective for the enzyme
B-lactamase, the gene for which is contained in the plasmid
pBR322. The method uses a procedure to screen for mutant clones which depends on the DNA in the various colonies and not on the properties of the mutant protein; the method can, therefore, be widely applied and does not require, in each separate case, the development of a screening procedure which depends on some phenotypic difference between mutant and wild-type protein
Quantification of Soyasaponins I and βg in Italian Lentil Seeds by Solid Phase Extraction (SPE) and High Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS)
Epidemiological and molecular assessment of a measles outbreak in a highly vaccinated population of northeast Italy.
SUMMARYTwo distinct measles outbreaks, unrelated from the epidemiological point of view but caused by genetically related strains, occurred in the Friuli Venezia Giulia region of northeastern Italy. Forty-two cases were reported during the period April–May 2008. In the first outbreak the index case was a teacher who introduced the virus into the Pordenone area, involving eight adolescents and young adults. The other concomitant outbreak occurred in the city of Trieste with 33 cases. The containment of the epidemics can be explained by the high MMR vaccine coverage in an area where the first dose was delivered to 93·4% and the second dose to 88·3% of the target children. Phylogenetic analysis of 14 measles virus strains showed that they belonged to a unique D4 genotype indistinguishable from the MVs/Enfield.GBR/14.07 strain, probably introduced from areas (i.e. Piedmont and Germany) where this genotype was present or had recently caused a large epidemic
Acessos de espécies silvestres no banco ativo de germoplasma de batata da embrapa clima temperado.
The effect of local thermal fluctuations on the folding kinetics: a study from the perspective of the nonextensive statistical mechanics
Protein folding is a universal process, very fast and accurate, which works
consistently (as it should be) in a wide range of physiological conditions. The
present work is based on three premises, namely: () folding reaction is a
process with two consecutive and independent stages, namely the search
mechanism and the overall productive stabilization; () the folding kinetics
results from a mechanism as fast as can be; and () at nanoscale
dimensions, local thermal fluctuations may have important role on the folding
kinetics. Here the first stage of folding process (search mechanism) is focused
exclusively. The effects and consequences of local thermal fluctuations on the
configurational kinetics, treated here in the context of non extensive
statistical mechanics, is analyzed in detail through the dependence of the
characteristic time of folding () on the temperature and on the
nonextensive parameter .The model used consists of effective residues
forming a chain of 27 beads, which occupy different sites of a D infinite
lattice, representing a single protein chain in solution. The configurational
evolution, treated by Monte Carlo simulation, is driven mainly by the change in
free energy of transfer between consecutive configurations. ...Comment: 19 pages, 3 figures, 1 tabl
Glass-ceramic sealants and steel interconnects: Accelerated interfacial stability and reactivity tests at high temperature
MeV range bremsstrahlung measurements of the runaway electron distribution function in disruption mitigation experiments at the ASDEX Upgrade and JET tokamaks
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