H2S in geothermal power plants: from waste to additional resource for energy and environment

Geothermal energy is a sustainable and clean energy source. Unfortunately, utilization of high-enthalpy geothermal systems is generally associated with emissions of gases like carbon dioxide (CO2), hydrogen sulfide (H2S), hydrogen (H2), nitrogen (N2), methane (CH4), and argon (Ar). The emission of some of these gases, particularly CO2, H2S and H2, is one of the main environmental concerns associated with the use of geothermal energy. The sequestration of these gases and their geological storage is the most diffuse viable option for reducing emissions. However, there is interesting technology, called AG2STM, that allows to convert H2S and CO2 into syngas. In this work, the match of this technology with geothermal power plant is analyzed as a new potential industrial route. The study is based on two different geothermal power plants located in Hellisheiði and Nesjavellirn (Iceland) that globally emit 61,800 t of CO2 and 28,200 t/y of H2S. The simulations provide some interesting results: (I) the total conversion of H2S that avoids its underground re-injection with the related environmental problem, (II) the reduction of CO2 emissions (about 8 %) and (III) the increasing of the global thermal energy produced with the same amount initial geothermal energy. The latter is due to the possibility to burn extra hydrogen coming from the AG2STM process. Finally, other advantages of this match are the production of an extra medium pressure steam and the possible reuse of the amount of H2 related to the geothermal plants emissions. Copyright © 2018, AIDIC Servizi S.r.l

Heavy residue excitation functions for the collisions 6, 7Li + 64Zn near the Coulomb barrier

Excitation functions for the production of heavy residues have been measured for the collisions 6, 7Li+64Zn at energies around and below the Coulomb barrier. The cross sections for heavy residue production have been measured using an activation technique, detecting off-line the characteristic atomic x-rays emitted in the electron capture decay of the reaction products. The experimental relative yields of the residues have been compared with statistical model calculations performed by using the code cascade. Such a comparison suggests that heavy residue production is dominated by complete fusion at above-barrier energies, whereas different processes like incomplete fusion and/or transfer become dominant in the sub-barrier energy region. The heavy residue excitation function ratio between the 6Li- and 7Li-induced collisions shows an increasing trend as the energy decreases below the barrier

BPAG1a and b Associate with EB1 and EB3 and Modulate Vesicular Transport, Golgi Apparatus Structure, and Cell Migration in C2.7 Myoblasts

BPAG1a and BPAG1b (BPAG1a/b) constitute two major isoforms encoded by the dystonin (Dst) gene and show homology with MACF1a and MACF1b. These proteins are members of the plakin family, giant multi-modular proteins able to connect the intermediate filament, microtubule and microfilament cytoskeletal networks with each other and to distinct cell membrane sites. They also serve as scaffolds for signaling proteins that modulate cytoskeletal dynamics. To gain better insights into the functions of BPAG1a/b, we further characterized their C-terminal region important for their interaction with microtubules and assessed the role of these isoforms in the cytoskeletal organization of C2.7 myoblast cells. Our results show that alternative splicing does not only occur at the 5′ end of Dst and Macf1 pre-mRNAs, as previously reported, but also at their 3′ end, resulting in expression of additional four mRNA variants of BPAG1 and MACF1. These isoform-specific C-tails were able to bundle microtubules and bound to both EB1 and EB3, two microtubule plus end proteins. In the C2.7 cell line, knockdown of BPAG1a/b had no major effect on the organization of the microtubule and microfilament networks, but negatively affected endocytosis and maintenance of the Golgi apparatus structure, which became dispersed. Finally, knockdown of BPAG1a/b caused a specific decrease in the directness of cell migration, but did not impair initial cell adhesion. These data provide novel insights into the complexity of alternative splicing of Dst pre-mRNAs and into the role of BPAG1a/b in vesicular transport, Golgi apparatus structure as well as in migration in C2.7 myoblasts

Microtubule Actin Crosslinking Factor 1 Regulates the Balbiani Body and Animal-Vegetal Polarity of the Zebrafish Oocyte

Although of fundamental importance in developmental biology, the genetic basis for the symmetry breaking events that polarize the vertebrate oocyte and egg are largely unknown. In vertebrates, the first morphological asymmetry in the oocyte is the Balbiani body, a highly conserved, transient structure found in vertebrates and invertebrates including Drosophila, Xenopus, human, and mouse. We report the identification of the zebrafish magellan (mgn) mutant, which exhibits a novel enlarged Balbiani body phenotype and a disruption of oocyte polarity. To determine the molecular identity of the mgn gene, we positionally cloned the gene, employing a novel DNA capture method to target region-specific genomic DNA of 600 kb for massively parallel sequencing. Using this technique, we were able to enrich for the genomic region linked to our mutation within one week and then identify the mutation in mgn using massively parallel sequencing. This is one of the first successful uses of genomic DNA enrichment combined with massively parallel sequencing to determine the molecular identity of a gene associated with a mutant phenotype. We anticipate that the combination of these technologies will have wide applicability for the efficient identification of mutant genes in all organisms. We identified the mutation in mgn as a deletion in the coding sequence of the zebrafish microtubule actin crosslinking factor 1 (macf1) gene. macf1 is a member of the highly conserved spectraplakin family of cytoskeletal linker proteins, which play diverse roles in polarized cells such as neurons, muscle cells, and epithelial cells. In mgn mutants, the oocyte nucleus is mislocalized; and the Balbiani body, localized mRNAs, and organelles are absent from the periphery of the oocyte, consistent with a function for macf1 in nuclear anchoring and cortical localization. These data provide the first evidence for a role for spectraplakins in polarization of the vertebrate oocyte and egg

Hippocampal pyramidal cells: the reemergence of cortical lamination

The increasing resolution of tract-tracing studies has led to the definition of segments along the transverse axis of the hippocampal pyramidal cell layer, which may represent functionally defined elements. This review will summarize evidence for a morphological and functional differentiation of pyramidal cells along the radial (deep to superficial) axis of the cell layer. In many species, deep and superficial sublayers can be identified histologically throughout large parts of the septotemporal extent of the hippocampus. Neurons in these sublayers are generated during different periods of development. During development, deep and superficial cells express genes (Sox5, SatB2) that also specify the phenotypes of superficial and deep cells in the neocortex. Deep and superficial cells differ neurochemically (e.g. calbindin and zinc) and in their adult gene expression patterns. These markers also distinguish sublayers in the septal hippocampus, where they are not readily apparent histologically in rat or mouse. Deep and superficial pyramidal cells differ in septal, striatal, and neocortical efferent connections. Distributions of deep and superficial pyramidal cell dendrites and studies in reeler or sparsely GFP-expressing mice indicate that this also applies to afferent pathways. Histological, neurochemical, and connective differences between deep and superficial neurons may correlate with (patho-) physiological phenomena specific to pyramidal cells at different radial locations. We feel that an appreciation of radial subdivisions in the pyramidal cell layer reminiscent of lamination in other cortical areas may be critical in the interpretation of studies of hippocampal anatomy and function