Implementasi Good Governance Untuk Meningkatkan Partisipasi Masyarakat Pada SMP Negeri 1 Lembang

Penyelenggaraan tata p emerintahan y ang haik merupakan salah satu isu nasional yang sedang dilangsungkan pemerintah saat ini. Namun demikian, pada level implementasi didana persekolahan, diduga hal ini masih jauh dari harapan, bahkan ada sinyalemen praktek-praktek yang salah berlangsung dalam penyelenggaraan sekolah. Penelitian ini berupaya melihat implementasi tata kelola yang baik dalam penyelenggaraan sekolah melalui pendekatan kualitatif. Temuan penelitian menunjukan, implementasi sembilan karakteristik good governance di sekolah semata-mata tidak didasarkan pada panggilan profesionalisme personil sekolah, tetapi lebih pada tuntutan internal dan eksternal s ekolah Dalam konteks itu, kepala sekofah berperan menjadi manajer dan leader, guru menjadi mitra kerja dan pelaksana, komite sekolah berperan dalam mendukung sumber d aya, mengawasi, memberikan pertimbangan, dan perantara. Faktor-faktor y a n g mendukung implementasi good governance d i sekolah adalah struktur sekolah, delapan nilai kerja yang dikembangkan, sikap warga sekolah dalam menjaga lingkungan fisik, dan hubungan dengan stakeholder. Mengantisipasi berbagai perkembangan lingkungan dan kompleksitas urusan di sekolah, melalui penelitian ini direkomendasikan agar sekolah mengembangkan learning mganisation, dan pimpinan sekolah memfasilitasi pemahaman yang lebih komprehensif mengenai good governance dan bagaimana mengimplementasikannya berdasarkan peran warga sekolah masing-masing

OPTIMIZATION OF WELD CRACK EXPANSION DEFECT OF WHEEL RIMS BY USING TAGUCHI APPROACH: A CASE STUDY

Abstract: For off-line quality control, Taguchi realized that the best opportunity to eliminate variation is during the design of a product and its manufacturing process. Consequently, he developed a strategy for quality engineering that can be used in both contexts. Analysis of varianc

Vitamin D attenuates sphingosine-1-phosphate (S1P)-mediated inhibition of extravillous trophoblast migration.

Failure of trophoblast invasion and remodelling of maternal blood vessels leads to the pregnancy complication pre-eclampsia (PE). In other systems, the sphingolipid, sphingosine-1-phosphate (S1P), controls cell migration therefore this study determined its effect on extravillous trophoblast (EVT) function.A transwell migration system was used to assess the behaviour of three trophoblast cell lines, Swan-71, SGHPL-4, and JEG3, and primary human trophoblasts in the presence or absence of S1P, S1P pathway inhibitors and 1,25(OH)2D3. QPCR and immunolocalisation were used to demonstrate EVT S1P receptor expression.EVTs express S1P receptors 1, 2 and 3. S1P inhibited EVT migration. This effect was abolished in the presence of the specific S1PR2 inhibitor, JTE-013 (p < 0.05 versus S1P alone) whereas treatment with the S1R1/3 inhibitor, FTY720, had no effect. In other cell types S1PR2 is regulated by vitamin D; here we found that treatment with 1,25(OH)2D3 for 48 or 72 h reduces S1PR2 (4-fold; <0.05), but not R1 and R3, expression. Moreover, S1P did not inhibit the migration of cells exposed to 1,25(OH)2D3 (p < 0.05).This study demonstrates that although EVT express three S1P receptor isoforms, S1P predominantly signals through S1PR2/Gα12/13 to activate Rho and thereby acts as potent inhibitor of EVT migration. Importantly, expression of S1PR2, and therefore S1P function, can be down-regulated by vitamin D. Our data suggest that vitamin D deficiency, which is known to be associated with PE, may contribute to the impaired trophoblast migration that underlies this condition

iMyoblasts for ex vivo and in vivo investigations of human myogenesis and disease modeling

Skeletal muscle myoblasts (iMyoblasts) were generated from human induced pluripotent stem cells (iPSCs) using an efficient and reliable transgene-free induction and stem cell selection protocol. Immunofluorescence, flow cytometry, qPCR, digital RNA expression profiling, and scRNA-Seq studies identify iMyoblasts as a PAX3+/MYOD1+ skeletal myogenic lineage with a fetal-like transcriptome signature, distinct from adult muscle biopsy myoblasts (bMyoblasts) and iPSC-induced muscle progenitors. iMyoblasts can be stably propagated for \u3e 12 passages or 30 population doublings while retaining their dual commitment for myotube differentiation and regeneration of reserve cells. iMyoblasts also efficiently xenoengrafted into irradiated and injured mouse muscle where they undergo differentiation and fetal-adult MYH isoform switching, demonstrating their regulatory plasticity for adult muscle maturation in response to signals in the host muscle. Xenograft muscle retains PAX3+ muscle progenitors and can regenerate human muscle in response to secondary injury. As models of disease, iMyoblasts from individuals with Facioscapulohumeral Muscular Dystrophy revealed a previously unknown epigenetic regulatory mechanism controlling developmental expression of the pathological DUX4 gene. iMyoblasts from Limb-Girdle Muscular Dystrophy R7 and R9 and Walker Warburg Syndrome patients modeled their molecular disease pathologies and were responsive to small molecule and gene editing therapeutics. These findings establish the utility of iMyoblasts for ex vivo and in vivo investigations of human myogenesis and disease pathogenesis and for the development of muscle stem cell therapeutics

Uptake of plasmodium falciparum gametocytes during mosquito bloodmeal by direct and membrane feeding

Plasmodium falciparum remains one of the leading causes of child mortality, and nearly half of the world’s population is at risk of contracting malaria. While pathogenesis results from replication of asexual forms in human red blood cells, it is the sexually differentiated forms, gametocytes, which are responsible for the spread of the disease. For transmission to succeed, both mature male and female gametocytes must be taken up by a female Anopheles mosquito during its blood meal for subsequent differentiation into gametes and mating inside the mosquito gut. Observed circulating numbers of gametocytes in the human host are often surprisingly low. A pre-fertilization behavior, such as skin sequestration, has been hypothesized to explain the efficiency of human-to-mosquito transmission but has not been sufficiently tested due to a lack of appropriate tools. In this study, we describe the optimization of a qPCR tool that enables the relative quantification of gametocytes within very small input samples. Such a tool allows for the quantification of gametocytes in different compartments of the host and the vector that could potentially unravel mechanisms that enable highly efficient malaria transmission. We demonstrate the use of our gametocyte quantification method in mosquito blood meals from both direct skin feeding on Plasmodium gametocyte carriers and standard membrane feeding assay. Relative gametocyte abundance was not different between mosquitoes fed through a membrane or directly on the skin suggesting that there is no systematic enrichment of gametocytes picked up in the skin

Anisotropic pH-Responsive Hydrogels Containing Soft or Hard Rod-Like Particles Assembled Using Low Shear

A simple and versatile low-shear approach for assembling hydrogels containing aligned rod-like particles (RLPs) that are birefringent and exhibit pH-triggered anisotropic swelling is developed. Anisotropic composite hydrogels are prepared by applying low shear (0.1 s–1) to mixtures of pH-responsive nanogels (NGs) and RLPs. The NGs, which contained high methacrylic acid contents, acted as both shear transfer vehicles and macro-cross-linkers for anisotropic gel formation. Three model RLP systems are investigated: (i) soft triblock copolymer worms, (ii) stiff self-assembled β-sheet peptide fibers, and (iii) ultrahigh modulus nanocrystalline cellulose fibers. RLP alignment was confirmed using polarized light imaging, atomic force microscopy, and small-angle X-ray scattering as well as modulus and anisotropic swelling experiments. Unexpectedly, the composite gel containing the soft copolymer worms showed the most pronounced anisotropy swelling. The copolymer worms enabled higher RLP loadings than was possible for the stiffer RLPs. For fixed RLP loading, the extent of anisotropic swelling increased with intra-RLP bonding strength. The facile and versatile approach to anisotropic gel construction demonstrated herein is expected to enable new applications for strain sensing or biomaterials for soft tissue repair

Optimasi micro frontend website untuk meningkatkan load times: teknik, tantangan, dan best practice

In recent years, there has been an increasing popularity of the micro frontend architecture due to its implementation by large companies such as IKEA, Starbucks, and Amazon. Due to its &nbsp;characteristics that similar to microservices, this architecture started to be implemented by various companies to improve their developer experience. However, this architecture has some issues, one of which is the performance of page load time. The objective of this research is to find and determine the best practices for optimizing the page load time of micro frontend applications and to identify the challenge involved. The research is conducted by implementing optimization techniques such as code splitting, lazy loading, tree shaking, minification, and utility modules to micro frontend website. After that, the website is tested with a sample size of 200 which determined by using Lemeshow formula. The research is conducted in both local and server environments using the Google Chrome browser and used "fully loaded" metric. The research use a simple Enterprise Resource Planning (ERP) application consisting of five micro frontends built with React, Vue, and Angular frameworks. The experimental results show that implementing all of the optimization techniques on all micro frontends can improve the application's page load time performance by 31.79% in the local and 47.5% in the server environment.Dalam beberapa tahun terakhir, terjadi peningkatan popularitas dari arsitektur micro frontend dikarenakan mulai diimplementasikan oleh perusahaan besar seperti IKEA, Starbucks, dan Amazon. Karakteristiknya yang menyerupai microservice membuat arsitektur ini mulai banyak diterapkan untuk meningkatkan developer experience. Namun, arsitektur ini memiliki beberapa masalah, salah satunya adalah performa page load time yang rendah. Tujuan dari dilakukannya penelitian ini adalah untuk menentukan bagaimana best practice dalam mengoptimasi performa page load time dari aplikasi micro frontend. Penelitian dilakukan dengan mengimplementasikan teknik optimasi seperti code splitting, lazy loading, tree shaking, minification, dan utility module kepada setiap micro frontend yang dimiliki oleh suatu website, kemudian dilakukan pengujian sebanyak 200 kali yang didapatkan menggunakan formula Lemeshow di local dan server environment menggunakan browser Google Chrome dengan metrik fully loaded, yaitu ukuran waktu yang dibutuhkan suatu website untuk memuat seluruh resources yang digunakan oleh website tersebut. Penelitian dilakukan pada aplikasi Enterprise Resources Planning (ERP) yang terdiri dari lima micro frontend dengan framework React, Vue, dan Angular. Hasil eksperimen yang dilakukan menunjukkan bahwa mengimplementasikan setiap teknik optimasi pada seluruh micro frontend dapat meningkatkan performa page load time aplikasi sebesar 31,79% pada local dan 47,5% pada server environment

Tumor Suppressor Protein p53 Recruits Human Sin3B/HDAC1 Complex for Down-Regulation of Its Target Promoters in Response to Genotoxic Stress

Master regulator protein p53, popularly known as the “guardian of genome” is the hub for regulation of diverse cellular pathways. Depending on the cell type and severity of DNA damage, p53 protein mediates cell cycle arrest or apoptosis, besides activating DNA repair, which is apparently achieved by regulation of its target genes, as well as direct interaction with other proteins. p53 is known to repress target genes via multiple mechanisms one of which is via recruitment of chromatin remodelling Sin3/HDAC1/2 complex. Sin3 proteins (Sin3A and Sin3B) regulate gene expression at the chromatin-level by serving as an anchor onto which the core Sin3/HDAC complex is assembled. The Sin3/HDAC co-repressor complex can be recruited by a large number of DNA-binding transcription factors. Sin3A has been closely linked to p53 while Sin3B is considered to be a close associate of E2Fs. The theme of this study was to establish the role of Sin3B in p53-mediated gene repression. We demonstrate a direct protein-protein interaction between human p53 and Sin3B (hSin3B). Amino acids 1–399 of hSin3B protein are involved in its interaction with N-terminal region (amino acids 1–108) of p53. Genotoxic stress induced by Adriamycin treatment increases the levels of hSin3B that is recruited to the promoters of p53-target genes (HSPA8, MAD1 and CRYZ). More importantly recruitment of hSin3B and repression of the three p53-target promoters upon Adriamycin treatment were observed only in p53+/+ cell lines. Additionally an increased tri-methylation of the H3K9 residue at the promoters of HSPA8 and CRYZ was also observed following Adriamycin treatment. The present study highlights for the first time the essential role of Sin3B as an important associate of p53 in mediating the cellular responses to stress and in the transcriptional repression of genes encoding for heat shock proteins or proteins involved in regulation of cell cycle and apoptosis